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Recently, the remarkable properties of silk fibroin protein have been studied, with biomedical and tissue engineering applications in mind, across a number of medical and surgical disciplines. The present study examines the use of silk fibroin for its potential suitability as an alternative graft in myringoplasty surgery by investigating the growth and proliferation of human tympanic membrane keratinocytes on a silk fibroin scaffold in vitro. Light microscopy, immunofluorescent staining, and confocal imaging all reveal promising preliminary results. The biocompatibility, transparency, stability, high tensile strength, and biodegradability of fibroin make this biomaterial an attractive option to study for this utility.<\/jats:p>","DOI":"10.1016\/j.otohns.2009.06.746","type":"journal-article","created":{"date-parts":[[2009,9,7]],"date-time":"2009-09-07T04:28:24Z","timestamp":1252297704000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Preliminary results of the application of a silk fibroin scaffold to otology"],"prefix":"10.1002","volume":"142","author":[{"given":"Brett","family":"Levin","sequence":"first","affiliation":[{"name":"Ear Science Institute of Australia and Ear Sciences Centre, School of Surgery, The University of Western Australia"},{"name":"Sir Charles Gairdner Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharon","family":"Leanne Redmond","sequence":"additional","affiliation":[{"name":"Ear Science Institute of Australia and Ear Sciences Centre, School of Surgery, The University of Western Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rangam","family":"Rajkhowa","sequence":"additional","affiliation":[{"name":"Centre for Material and Fibre Innovation Deakin University Geelong Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert Henry","family":"Eikelboom","sequence":"additional","affiliation":[{"name":"Ear Science Institute of Australia and Ear Sciences Centre, School of Surgery, The University of Western Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert Jeffery","family":"Marano","sequence":"additional","affiliation":[{"name":"Ear Science Institute of Australia and Ear Sciences Centre, School of Surgery, The University of Western Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcus David","family":"Atlas","sequence":"additional","affiliation":[{"name":"Ear Science Institute of Australia and Ear Sciences Centre, School of Surgery, The University of Western Australia"},{"name":"Sir Charles Gairdner Hospital"},{"name":"St. John of God Hospital Perth Western Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,3]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022215106000697"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2273.2003.00716.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(02)00353-8"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2007.00251.x"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.progpolymsci.2007.05.013"}],"container-title":["Otolaryngology\u2013Head and Neck Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.otohns.2009.06.746","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aao-hnsfjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.otohns.2009.06.746","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:00:45Z","timestamp":1760954445000},"score":1,"resource":{"primary":{"URL":"https:\/\/aao-hnsfjournals.onlinelibrary.wiley.com\/doi\/10.1016\/j.otohns.2009.06.746"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3]]},"references-count":5,"journal-issue":{"issue":"S3","published-print":{"date-parts":[[2010,3]]}},"alternative-id":["10.1016\/j.otohns.2009.06.746"],"URL":"https:\/\/doi.org\/10.1016\/j.otohns.2009.06.746","archive":["Portico"],"relation":{},"ISSN":["0194-5998","1097-6817"],"issn-type":[{"type":"print","value":"0194-5998"},{"type":"electronic","value":"1097-6817"}],"subject":[],"published":{"date-parts":[[2010,3]]},"assertion":[{"value":"2010-03-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}