{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:04:35Z","timestamp":1760983475381,"version":"build-2065373602"},"reference-count":21,"publisher":"Wiley","issue":"S3","license":[{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:p>Objective: Perineural invasion (PNI) in cutaneous squamous cell carcinoma of the head and neck (CSCCHN) carries poor prognosis. Tumor markers associated with neurotropism in CSCCHN have not been identified. Our objective was to study the expression of \u03b1B\u2010crystallin in CSCCHN with neurotropism.<\/jats:p><jats:p>Study Design: Cross\u2010sectional review of pathologic specimens.<\/jats:p><jats:p>Setting: Tertiary care center.<\/jats:p><jats:p>Subjects and Methods: Tissue from patients with CSCCHN with clinical PNI who underwent surgery between 1998 and 2005 was immunostained for \u03b1B\u2010crystallin. In addition, non\u2010PNI CSCCHN and normal nerve sections were also stained. Staining intensity was calculated by the histologic, or H, score (product of the intensity and proportion of tumor cells stained). The H\u2010score ranged from 0.0 to 3.0, with 0\u00a0indicating negative staining in all cells and 3.0\u00a0indicating strong staining in 100 percent of cells.<\/jats:p><jats:p>Results: Tissue was available in 15 clinical PNI CSCCHN patients. The analysis was also carried out in 14\u00a0non\u2010PNI patients matched by stage and four normal greater auricular nerve (GAN) sections. The mean H\u2010score was 0.56 for CSCCHN with PNI, 1.06 for non\u2010PNI CSCCHN, and 3.0 for normal nerves. The difference in H\u2010score between PNI and non\u2010PNI CSCCHN was statistically significant (P = 0.04).<\/jats:p><jats:p>Conclusion: CSCCHN with clinical PNI has decreased staining for \u03b1B\u2010crystallin. This finding further demonstrates the differences between clinical PNI and non\u2010PNI CSCCHN tumors. Additional studies are required to identify cell surface markers expressed by CSCCHN that confer neurotropism capabilities.<\/jats:p>","DOI":"10.1016\/j.otohns.2009.12.001","type":"journal-article","created":{"date-parts":[[2010,2,20]],"date-time":"2010-02-20T04:39:09Z","timestamp":1266640749000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Reduced \u03b1B\u2010crystallin staining in perineural invasion of head and neck cutaneous squamous cell carcinoma"],"prefix":"10.1002","volume":"142","author":[{"given":"C. Arturo","family":"Solares","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Medical College of Georgia Augusta GA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Glen M.","family":"Boyle","sequence":"additional","affiliation":[{"name":"Queensland Institute of Medical Research Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian","family":"Brown","sequence":"additional","affiliation":[{"name":"Sullivan and Nicolaides Pathology Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter G.","family":"Parsons","sequence":"additional","affiliation":[{"name":"Queensland Institute of Medical Research Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benedict","family":"Panizza","sequence":"additional","affiliation":[{"name":"Princess Alexandra Hospital Skull Base Unit Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,3]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1067\/mhn.2000.105057"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-199905000-00026"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.21037"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI25888"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0360-3016(99)00533-7"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470123157.ch5"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(89)80215-3"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.MLG.0000164715.86240.55"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/00313020802198010"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2006.09.007"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.7.19.6775"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/1747-1028-2-2"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.21.10449"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)53882-5"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.2.221"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.3.659"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1997.0358"},{"key":"e_1_2_1_19_1","first-page":"345","article-title":"Accumulation of alpha B\u2010crystallin in central nervous system glia and neurons in pathologic conditions","volume":"140","author":"Iwaki T","year":"1992","journal-title":"Am J Pathol"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711660110"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.96.12.4343"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1998.0892"}],"container-title":["Otolaryngology\u2013Head and Neck Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.otohns.2009.12.001","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aao-hnsfjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.otohns.2009.12.001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:00:43Z","timestamp":1760954443000},"score":1,"resource":{"primary":{"URL":"https:\/\/aao-hnsfjournals.onlinelibrary.wiley.com\/doi\/10.1016\/j.otohns.2009.12.001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3]]},"references-count":21,"journal-issue":{"issue":"S3","published-print":{"date-parts":[[2010,3]]}},"alternative-id":["10.1016\/j.otohns.2009.12.001"],"URL":"https:\/\/doi.org\/10.1016\/j.otohns.2009.12.001","archive":["Portico"],"relation":{},"ISSN":["0194-5998","1097-6817"],"issn-type":[{"type":"print","value":"0194-5998"},{"type":"electronic","value":"1097-6817"}],"subject":[],"published":{"date-parts":[[2010,3]]},"assertion":[{"value":"2010-03-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}