{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T16:24:52Z","timestamp":1774542292685,"version":"3.50.1"},"reference-count":14,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2010,6,1]],"date-time":"2010-06-01T00:00:00Z","timestamp":1275350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>The objectives of this study were to 1) determine differences in lifetime fluorescence between normal and malignant tissue of the upper aerodigestive tract, and 2) evaluate the potential of time\u2010resolved laser\u2010induced fluorescence spectroscopy (TR\u2010LIFS) as a diagnostic instrument for head and neck squamous cell carcinoma (HNSCC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Cross\u2010sectional study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>University\u2010based medical center.<\/jats:p><\/jats:sec><jats:sec><jats:title>Subjects and Methods<\/jats:title><jats:p>Nine patients with suspected HNSCC were included. In the operating room, a nitrogen pulse laser (337\u00a0nm, 700\u2010picosecond pulse width) was used to induce tissue autofluorescence of normal tissue and suspected malignant lesions. Spectral intensities and time\u2010domain measurements were obtained and compared with the histopathology at each site. A total of 53 sites were measured. The fluorescence parameters that provided the most discrimination were determined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Differences in spectral intensities allowed for discrimination between malignant and normal tissue. The spectral intensity of malignant tissue was lower than that of normal tissue, and a shift of peak intensity to a longer wavelength was observed in the normalized spectrum of malignant tissue in the range of 360 to approximately 660\u00a0nm. Multiple time\u2010resolved fluorescence parameters provided the best diagnostic discrimination between normal tissue and carcinoma, including average lifetimes (i.e., at 390\u00a0nm: 1.7 \u00b1 0.06\u00a0ns [not significant] for normal and 1.3 \u00b1 0.06\u00a0ns for tumor, <jats:italic>P<\/jats:italic> = 0.0025) and the second\u2010order Laguerre expansion coefficient (LEC\u20102) (i.e., at 460\u00a0nm: 0.135 \u00b1 0.001 for normal and 0.155 \u00b1 0.007 for tumor, <jats:italic>P<\/jats:italic> &lt; 0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings highlight some of the differences in lifetime fluorescence between normal and malignant tissue. TR\u2010LIFS has potential as a noninvasive diagnostic technique for HNSCC.<\/jats:p><\/jats:sec>","DOI":"10.1016\/j.otohns.2010.02.005","type":"journal-article","created":{"date-parts":[[2010,5,20]],"date-time":"2010-05-20T08:29:25Z","timestamp":1274344165000},"page":"838-844","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Time\u2010resolved laser\u2010induced fluorescence spectroscopy as a diagnostic instrument in head and neck carcinoma"],"prefix":"10.1002","volume":"142","author":[{"given":"Jeremy D.","family":"Meier","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery University of California Davis Sacramento CA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongtao","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of California Davis CA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of California Davis CA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinghua","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of California Davis CA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nisa","family":"Hatami","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of California Davis CA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian","family":"Poirier","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of California Davis Sacramento CA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Marcu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of California Davis CA"},{"name":"NSF Center for Biophotonics Science &amp; Technology Sacramento CA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. 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