{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T16:47:46Z","timestamp":1771951666400,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2010,9,1]],"date-time":"2010-09-01T00:00:00Z","timestamp":1283299200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:sec><jats:title>OBJECTIVE<\/jats:title><jats:p>Age\u2010related hearing loss (ARHL) is characterized by gradual, progressive sensorineural hearing loss, which impairs communication, lending to clinical depression and social withdrawal. There are currently no effective treatments for ARHL. The purpose of this study is to evaluate the potential of a combination antioxidant therapy in preventing ARHL.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN<\/jats:title><jats:p>Randomized controlled trial.<\/jats:p><\/jats:sec><jats:sec><jats:title>SETTING<\/jats:title><jats:p>Animal study.<\/jats:p><\/jats:sec><jats:sec><jats:title>SUBJECTS AND METHODS<\/jats:title><jats:p>C57BL\/6\u00a0mice, a recognized animal model of ARHL, were assigned to one of three groups: early treatment (n = 12), late treatment (n = 9), or control group (n = 9). Treatment groups of mice were fed with a combination agent comprising six antioxidant agents that target four sites within the oxidative pathway: L\u2010cysteine\u2010glutathione mixed disulfide, ribose\u2010 cysteine, NW\u2010nitro\u2010L\u2010arginine methyl ester, vitamin B12, folate, and ascorbic acid. Auditory brainstem response (ABR) thresholds were recorded at baseline and every three months following initiation of treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Threshold shifts from baseline were decreased in the treatment groups when compared to the control group at all tested frequencies (<jats:italic>P<\/jats:italic> &lt; 0.001). The ABR threshold shift at 12\u00a0months of age for the control group was 34.7\u00a0dB with a 95% confidence interval (CI) of \u00b1 1.6. The mean threshold shifts for the early and late treatment groups were 7.5\u00a0dB (\u00b10.87, 95% CI) and 9.2\u00a0dB (\u00b11.6, 95% CI).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Combination antioxidant therapy effectively decreased threshold shifts on ABR within an animal model of ARHL. Combination antioxidant therapy, with further research and investigation, may provide a safe and cost\u2010effective method of preventing presbycusis in the growing elderly population.<\/jats:p><jats:p>\u00a9 2010 American Academy of Otolaryngology\u2010Head and Neck Surgery Foundation. All rights reserved.<\/jats:p><\/jats:sec>","DOI":"10.1016\/j.otohns.2010.04.266","type":"journal-article","created":{"date-parts":[[2010,8,18]],"date-time":"2010-08-18T06:28:35Z","timestamp":1282112915000},"page":"429-434","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":70,"title":["A combination antioxidant therapy prevents age\u2010related hearing loss in C57BL\/6\u00a0mice"],"prefix":"10.1002","volume":"143","author":[{"given":"Selena E. Heman","family":"Ackah","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery University of Minnesota Minneapolis MN"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven K.","family":"Juhn","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery University of Minnesota Minneapolis MN"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tina C.","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery University of Minnesota Minneapolis MN"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy S.","family":"Wiedmann","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics University of Minnesota Minneapolis MN"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,9]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"US Census BureauState Interim Population Projections by Age and Sex: 2004\u20102030 (April 2005). 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