{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T02:25:30Z","timestamp":1767925530842,"version":"3.49.0"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2010,12,1]],"date-time":"2010-12-01T00:00:00Z","timestamp":1291161600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aao-hnsfjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Otolaryngol.--head neck surg."],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:sec><jats:title>OBJECTIVE<\/jats:title><jats:p>Pepsin detection in throat sputum has been posited as a reliable biological marker of laryngopharyngeal reflux disease (LPRD). This study was designed to further correlate pepsin concentration with symptoms and signs of LPRD.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN<\/jats:title><jats:p>Cross\u2010sectional study.<\/jats:p><\/jats:sec><jats:sec><jats:title>SETTING<\/jats:title><jats:p>Nanfang Hospital of Southern Medical University.<\/jats:p><\/jats:sec><jats:sec><jats:title>SUBJECTS AND METHODS<\/jats:title><jats:p>Fifty\u2010six laryngitis patients were divided into a reflux laryngitis group and a chronic laryngitis group based on the reflux symptom index (RSI), reflux finding score (RFS), and proton pump inhibitor treatment for two weeks. Oral and hypopharyngeal secretions from the study patients and from 15 healthy subjects were collected. Thirty\u2010six obstructive sleep apnea (OSA) patients were divided into a mild\u2010moderate group and a severe group by the apnea\u2010hypopnea index (AHI). Bedtime and first\u2010morning oral secretions were collected. Enzyme\u2010linked immunosorbent assay was used to measure the pepsin concentration.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>In laryngitis patients, the total score of RSI and RFS (<jats:italic>P<\/jats:italic> &lt; 0.05), and the symptoms, including clearing throat often, coughing, and sensing a lump in the throat (<jats:italic>P<\/jats:italic> &lt; 0.006), were more severe in the pepsin\u2010positive group. No significant differences were found between the oral and hypopharyngeal secretions. In OSA, pepsin levels in the first\u2010morning oral secretions were correlated with AHI, mean SaO<jats:sub>2<\/jats:sub>, and mini SaO<jats:sub>2<\/jats:sub> (<jats:italic>P<\/jats:italic> &lt; 0.01). However, RSIs were not significantly correlated with these indicators.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Higher levels of pepsin in sputum were associated with higher RSI and RFS in cases of laryngitis. There was no relationship between pepsin levels and RSI in cases of OSA. There were no differences of pepsin concentration in sputum collection methods or in collection timing.<\/jats:p><\/jats:sec>","DOI":"10.1016\/j.otohns.2010.08.018","type":"journal-article","created":{"date-parts":[[2010,11,24]],"date-time":"2010-11-24T04:25:59Z","timestamp":1290572759000},"page":"765-771","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":55,"title":["Correlation of pepsin\u2010measured laryngopharyngeal reflux disease with symptoms and signs"],"prefix":"10.1002","volume":"143","author":[{"given":"Lu","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Nanfang Hospital of Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Nanfang Hospital of Southern Medical University 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