{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T02:56:32Z","timestamp":1783479392872,"version":"3.55.0"},"reference-count":18,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2017,11,24]],"date-time":"2017-11-24T00:00:00Z","timestamp":1511481600000},"content-version":"am","delay-in-days":296,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["1523410"],"award-info":[{"award-number":["1523410"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Parasitology International"],"published-print":{"date-parts":[[2017,2]]},"DOI":"10.1016\/j.parint.2016.08.002","type":"journal-article","created":{"date-parts":[[2016,8,7]],"date-time":"2016-08-07T14:55:36Z","timestamp":1470581736000},"page":"1003-1007","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"title":["Real-time PCR detection and phylogenetic relationships of Neorickettsia spp. in digeneans from Egypt, Philippines, Thailand, Vietnam and the United States"],"prefix":"10.1016","volume":"66","author":[{"given":"Stephen E.","family":"Greiman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jefferson A.","family":"Vaughan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasha","family":"Elmahy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Poom","family":"Adisakwattana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen","family":"Van Ha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J.","family":"Fayton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal I.","family":"Khalil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasyl V.","family":"Tkach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.parint.2016.08.002_bb0005","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/B978-0-12-398457-9.00003-2","article-title":"Neorickettsial endosymbionts of the Digenea: diversity, transmission and distribution","volume":"79","author":"Vaughan","year":"2012","journal-title":"Adv. Parasitol."},{"key":"10.1016\/j.parint.2016.08.002_bb0010","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0098453","article-title":"Large scale screening of digeneans for Neorickettsia endosymbionts using real-time PCR reveals new Neorickettsia genotypes, host associations and geographic records","volume":"9","author":"Greiman","year":"2014","journal-title":"PLoS ONE"},{"key":"10.1016\/j.parint.2016.08.002_bb0015","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1007\/s00792-009-0252-4","article-title":"Characterization of the intestinal microbiota of two Antarctic notothenioid fish species","volume":"13","author":"Ward","year":"2009","journal-title":"Extremophiles"},{"key":"10.1016\/j.parint.2016.08.002_bb0020","doi-asserted-by":"crossref","first-page":"285","DOI":"10.2478\/s11686-012-0043-4","article-title":"New genetic lineages, host associations and circulation pathways of Neorickettsia endosymbionts of digeneans","volume":"57","author":"Tkach","year":"2012","journal-title":"Acta Parasitol."},{"key":"10.1016\/j.parint.2016.08.002_bb0025","first-page":"147","article-title":"A new method of DNA extraction from the ethanol-fixed parasitic worms","volume":"44","author":"Tkach","year":"1999","journal-title":"Acta Parasitol."},{"key":"10.1016\/j.parint.2016.08.002_bb0030","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1186\/1756-3305-6-303","article-title":"Transmission rates of the bacterial endosymbiont, Neorickettsia risticii, during the asexual phase of its digenean host, Plagiorchis elegans, within naturally infected lymnaeid snails","volume":"6","author":"Greiman","year":"2013","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.parint.2016.08.002_bb0035","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.exppara.2015.06.015","article-title":"Laboratory maintenance of the bacterial endosymbiont, Neorickettsia sp., through the life cycle of a digenean, Plagiorchis elegans","volume":"157","author":"Greiman","year":"2015","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.parint.2016.08.002_bb0040","first-page":"95","article-title":"BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT","volume":"41","author":"Hall","year":"1999","journal-title":"Nucleic Acids Symp. Ser."},{"key":"10.1016\/j.parint.2016.08.002_bb0045","series-title":"MacClade 4: Analysis of Phylogeny and Character Evolution. Version 4.08a","author":"Maddison","year":"2005"},{"key":"10.1016\/j.parint.2016.08.002_bb0050","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1093\/bioinformatics\/17.8.754","article-title":"MRBAYES: Bayesian inference of phylogeny","volume":"17","author":"Huelsenbeck","year":"2001","journal-title":"Bioinformatics"},{"key":"10.1016\/j.parint.2016.08.002_bb0055","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1073\/pnas.78.1.454","article-title":"Estimation of evolutionary distances between homologous nucleotide sequences","volume":"78","author":"Kimura","year":"1981","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.parint.2016.08.002_bb0060","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1080\/10635150390235520","article-title":"A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood","volume":"52","author":"Guindon","year":"2003","journal-title":"Syst. Biol."},{"key":"10.1016\/j.parint.2016.08.002_bb0065","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1093\/molbev\/msn083","article-title":"jModelTest: phylogenetic model averaging","volume":"25","author":"Posada","year":"2008","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.parint.2016.08.002_bb0070","series-title":"Keys to the Trematoda","first-page":"527","article-title":"Family Lecithodendriidae Luhe, 1901","volume":"vol. 3","author":"Lotz","year":"2008"},{"key":"10.1016\/j.parint.2016.08.002_bb0075","first-page":"37","article-title":"One new species of Saccocoelioides (Digenea: Haploporidae) from Liza carinatus in Taiwan Strait","volume":"21","author":"Liu","year":"2002","journal-title":"J. Oceanogr."},{"key":"10.1016\/j.parint.2016.08.002_bb0080","first-page":"62","article-title":"An additional genus and two additional species of Forticulcitinae (Digenea: Haploporidae)","author":"Andres","year":"2015","journal-title":"Folia Parasitol."},{"key":"10.1016\/j.parint.2016.08.002_bb0085","doi-asserted-by":"crossref","first-page":"2145","DOI":"10.1099\/00207713-51-6-2145","volume":"51","author":"Dumler","year":"2001","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"10.1016\/j.parint.2016.08.002_bb0090","first-page":"51","article-title":"Rickettsial mononucleosis (synonyms: Hyuganetsu, kagaminetsu)","volume":"31","author":"Fukuda","year":"1958","journal-title":"Kitasato Arch. Exp. Med."}],"container-title":["Parasitology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1383576916301672?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1383576916301672?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T10:28:03Z","timestamp":1761301683000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1383576916301672"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2]]},"references-count":18,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,2]]}},"alternative-id":["S1383576916301672"],"URL":"https:\/\/doi.org\/10.1016\/j.parint.2016.08.002","relation":{},"ISSN":["1383-5769"],"issn-type":[{"value":"1383-5769","type":"print"}],"subject":[],"published":{"date-parts":[[2017,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Real-time PCR detection and phylogenetic relationships of Neorickettsia spp. in digeneans from Egypt, Philippines, Thailand, Vietnam and the United States","name":"articletitle","label":"Article Title"},{"value":"Parasitology International","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.parint.2016.08.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier Ireland Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}