{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T06:18:50Z","timestamp":1789712330329,"version":"4.0.1"},"reference-count":19,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Parasitology International"],"published-print":{"date-parts":[[2018,2]]},"DOI":"10.1016\/j.parint.2017.03.001","type":"journal-article","created":{"date-parts":[[2017,3,11]],"date-time":"2017-03-11T01:30:40Z","timestamp":1489195840000},"page":"34-37","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":23,"title":["Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION\u2122 nanopore sequencing"],"prefix":"10.1016","volume":"67","author":[{"given":"Kazuo","family":"Imai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norihito","family":"Tarumoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyoko","family":"Amo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoya","family":"Sakamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Kosaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuyuki","family":"Kato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kei","family":"Mikita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Sakai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Murakami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutaka","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigefumi","family":"Maesaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuya","family":"Maeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"10","key":"10.1016\/j.parint.2017.03.001_bb0005","first-page":"1712","article-title":"Ongoing epidemic of cutaneous leishmaniasis among Syrian refugees","volume":"20","author":"Saroufim","year":"2014","journal-title":"Lebanon, Emer. Infect. Dis."},{"issue":"6","key":"10.1016\/j.parint.2017.03.001_bb0010","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0129418","article-title":"Epidemiology of imported leishmaniasis in Italy: implications for a European endemic country","volume":"10","author":"Di Muccio","year":"2015","journal-title":"PLoS One"},{"issue":"2","key":"10.1016\/j.parint.2017.03.001_bb0015","doi-asserted-by":"crossref","first-page":"181","DOI":"10.2307\/3285616","article-title":"PCR detection and identification of Leishmania parasites in clinical specimens in Ecuador: a comparison with classical diagnostic methods","volume":"85","author":"Aviles","year":"1999","journal-title":"J. Parasitol."},{"issue":"3","key":"10.1016\/j.parint.2017.03.001_bb0020","doi-asserted-by":"crossref","first-page":"294","DOI":"10.4269\/ajtmh.2004.70.294","article-title":"A sensitive new microculture method for diagnosis of cutaneous leishmaniasis","volume":"70","author":"Allahverdiyev","year":"2004","journal-title":"Am.J.Trop. Med. Hyg."},{"issue":"6","key":"10.1016\/j.parint.2017.03.001_bb0025","doi-asserted-by":"crossref","first-page":"847","DOI":"10.4269\/ajtmh.2008.79.847","article-title":"Optimization of microculture and evaluation of miniculture for the isolation of Leishmania parasites from cutaneous lesions in Peru","volume":"79","author":"Boggild","year":"2008","journal-title":"Am.J.Trop. Med. Hyg."},{"issue":"1","key":"10.1016\/j.parint.2017.03.001_bb0030","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.actatropica.2006.07.001","article-title":"Clinical diagnosis of cutaneous leishmaniasis: a comparison study between standardized graded direct microscopy and ITS1-PCR of giemsa-stained smears","volume":"99","author":"Al-Jawabreh","year":"2006","journal-title":"Acta Trop."},{"issue":"4","key":"10.1016\/j.parint.2017.03.001_bb0035","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1128\/JCM.44.4.1435-1439.2006","article-title":"Comparison of PCR assays for diagnosis of cutaneous leishmaniasis","volume":"44","author":"Bensoussan","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.parint.2017.03.001_bb0040","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1186\/1756-3305-7-420","article-title":"Molecular diagnosis of cutaneous leishmaniasis and identification of the causative Leishmania species in Morocco by using three PCR-based assays","volume":"7","author":"Mouttaki","year":"2014","journal-title":"Parasit. Vectors"},{"issue":"4","key":"10.1016\/j.parint.2017.03.001_bb0045","doi-asserted-by":"crossref","first-page":"591","DOI":"10.4269\/ajtmh.2010.09-0369","article-title":"Development of a reverse transcriptase loop-mediated isothermal amplification (LAMP) assay for the sensitive detection of Leishmania parasites in clinical samples","volume":"82","author":"Adams","year":"2010","journal-title":"Am.J.Trop. Med. Hyg."},{"key":"10.1016\/j.parint.2017.03.001_bb0050","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.actatropica.2015.10.013","article-title":"A rapid molecular diagnosis of cutaneous leishmaniasis by colorimetric malachite green-loop-mediated isothermal amplification (LAMP) combined with an FTA card as a direct sampling tool","volume":"153","author":"Nzelu","year":"2016","journal-title":"Acta Trop."},{"issue":"6","key":"10.1016\/j.parint.2017.03.001_bb0055","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1016\/j.parint.2014.07.007","article-title":"The directs boil-LAMP method: a simple and rapid diagnostic method for cutaneous leishmaniasis","volume":"63","author":"Mikita","year":"2014","journal-title":"Parasitol. Int."},{"issue":"Pt 5","key":"10.1016\/j.parint.2017.03.001_bb0060","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1017\/S0031182004004792","article-title":"Sequence variation of the cytochrome b gene of various human infecting members of the genus Leishmania and their phylogeny","volume":"128","author":"Luyo-Acero","year":"2004","journal-title":"Parasitology"},{"issue":"30","key":"10.1016\/j.parint.2017.03.001_bb0065","doi-asserted-by":"crossref","first-page":"20543","DOI":"10.2807\/1560-7917.ES2013.18.30.20543","article-title":"Heat-shock protein 70 gene sequencing for Leishmania species typing in European tropical infectious disease clinics","volume":"18","author":"Van der Auwera","year":"2013","journal-title":"Euro Surveill."},{"issue":"7589","key":"10.1016\/j.parint.2017.03.001_bb0070","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1038\/nature16996","article-title":"Real-time, portable genome sequencing for ebola surveillance","volume":"530","author":"Quick","year":"2016","journal-title":"Nature"},{"issue":"5","key":"10.1016\/j.parint.2017.03.001_bb0075","doi-asserted-by":"crossref","first-page":"1666","DOI":"10.1128\/JCM.40.5.1666-1669.2002","article-title":"Real-time PCR for detection and quantitation of Leishmania in mouse tissues","volume":"40","author":"Nicolas","year":"2002","journal-title":"J. Clin. Microbiol."},{"issue":"14","key":"10.1016\/j.parint.2017.03.001_bb0080","doi-asserted-by":"crossref","first-page":"1891","DOI":"10.1017\/S0031182014001280","article-title":"Sensitive diagnosis of cutaneous leishmaniasis by lesion swab sampling coupled to qPCR","volume":"141","author":"Adams","year":"2014","journal-title":"Parasitology"},{"issue":"7","key":"10.1016\/j.parint.2017.03.001_bb0085","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0133759","article-title":"A simple and rapid identification method for Mycobacterium bovis BCG with loop-mediated isothermal amplification","volume":"10","author":"Kouzaki","year":"2015","journal-title":"PLoS One"},{"issue":"11","key":"10.1016\/j.parint.2017.03.001_bb0090","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.2169\/internalmedicine.54.4615","article-title":"PURE-LAMP procedure for the diagnosis of extrapulmonary tuberculosis: a case series","volume":"54","author":"Kouzaki","year":"2015","journal-title":"Intern. Med."},{"issue":"22","key":"10.1016\/j.parint.2017.03.001_bb0095","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkv773","article-title":"A novel method for the multiplexed target enrichment of MinION next generation sequencing libraries using PCR-generated baits","volume":"43","author":"Karamitros","year":"2015","journal-title":"Nucleic Acids Res."}],"container-title":["Parasitology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1383576917300430?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1383576917300430?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T10:28:42Z","timestamp":1761301722000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1383576917300430"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2]]},"references-count":19,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["S1383576917300430"],"URL":"https:\/\/doi.org\/10.1016\/j.parint.2017.03.001","relation":{},"ISSN":["1383-5769"],"issn-type":[{"value":"1383-5769","type":"print"}],"subject":[],"published":{"date-parts":[[2018,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION\u2122 nanopore sequencing","name":"articletitle","label":"Article Title"},{"value":"Parasitology International","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.parint.2017.03.001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Published by Elsevier Ireland Ltd.","name":"copyright","label":"Copyright"}]}}