{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:37:12Z","timestamp":1762079832627,"version":"build-2065373602"},"reference-count":30,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100008828","name":"Heilongjiang Bayi Agricultural University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008828","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Parasitology International"],"published-print":{"date-parts":[[2022,6]]},"DOI":"10.1016\/j.parint.2022.102555","type":"journal-article","created":{"date-parts":[[2022,2,5]],"date-time":"2022-02-05T02:52:41Z","timestamp":1644029561000},"page":"102555","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Identification of new polymorphic positions in rDNA sequences of the \u201cintermediate\u201d Fasciola forms"],"prefix":"10.1016","volume":"88","author":[{"given":"Minhao","family":"Zeng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuo","family":"Lan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinru","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingting","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiaocheng","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunren","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"11\u221212","key":"10.1016\/j.parint.2022.102555_bb0005","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1016\/j.ijpara.2005.07.010","article-title":"Fascioliasis and other plant-borne trematode zoonoses","volume":"35","author":"Mas-Coma","year":"2005","journal-title":"Int. J. Parasitol."},{"issue":"4","key":"10.1016\/j.parint.2022.102555_bb0010","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.pt.2008.01.001","article-title":"Food-borne parasitic zoonoses in China: perspective for control","volume":"24","author":"Zhou","year":"2008","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.parint.2022.102555_bb0015","first-page":"141","article-title":"Chapter 2. Fasciola, lymnaeids and human fascioliasis, with a global overview on disease transmission, epidemiology, evolutionary genetics, molecular epidemiology and control","volume":"69","author":"Mas-Coma","year":"2009","journal-title":"Adv. Parasitol."},{"key":"10.1016\/j.parint.2022.102555_bb0020","first-page":"356","article-title":"Fasciola hepatica infection in China","volume":"22","author":"Chen","year":"1991","journal-title":"Southeast Asian J. Trop. Med. Public Healt."},{"issue":"3\u20134","key":"10.1016\/j.parint.2022.102555_bb0025","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.vetpar.2006.04.008","article-title":"Survey of helminths in adult sheep in Heilongjiang Province, People's Republic of China","volume":"140","author":"Wang","year":"2006","journal-title":"Vet. Parasitol."},{"issue":"4","key":"10.1016\/j.parint.2022.102555_bb0030","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.1007\/s00436-013-3326-2","article-title":"Phylogenetic data suggest the reclassification of Fasciola jacksoni (Digenea: Fasciolidae) as Fascioloides jacksoni comb nov","volume":"112","author":"Heneberg","year":"2013","journal-title":"Parasitol. Res."},{"issue":"5","key":"10.1016\/j.parint.2022.102555_bb0035","first-page":"443","article-title":"Detection of difference among Fasciola hepatica and Fasciola gigantica with RAPD","volume":"21","author":"Xu","year":"2001","journal-title":"Chin. J. Vet. Sci."},{"issue":"5","key":"10.1016\/j.parint.2022.102555_bb0040","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1016\/S0020-7519(98)00037-X","article-title":"Triploid form of Fasciola in Japan: genetic relationships between Fasciola hepatica and Fasciola gigantica determined by ITS-2 sequence of nuclear rDNA","volume":"28","author":"Itagaki","year":"1998","journal-title":"Int. J. Parasitol."},{"issue":"3","key":"10.1016\/j.parint.2022.102555_bb0045","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/S1383-5769(00)00051-9","article-title":"Molecular evidence of natural hybridization between Fasciola hepatica and F. gigantica","volume":"49","author":"Agatsuma","year":"2000","journal-title":"Parasitol. Int."},{"issue":"5","key":"10.1016\/j.parint.2022.102555_bb0050","doi-asserted-by":"crossref","first-page":"453","DOI":"10.3347\/kjp.2018.56.5.453","article-title":"Distribution status of hybrid types in large liver flukes, Fasciola species (digenea: fasciolidae), from ruminants and humans in Vietnam","volume":"56","author":"Nguyen","year":"2018","journal-title":"Kor. J. Parasitol."},{"issue":"1","key":"10.1016\/j.parint.2022.102555_bb0055","first-page":"46","article-title":"Morphological and molecular discrimination of fasciola species isolated from domestic ruminants of Urmia City, Iran","volume":"10","author":"Yakhchali","year":"2015","journal-title":"Iran. J. Parasitol."},{"issue":"1","key":"10.1016\/j.parint.2022.102555_bb0060","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.meegid.2007.10.001","article-title":"First phenotypic description of Fasciola hepatica\/Fasciola gigantica intermediate Fasciolas from the human endemic area of the Nile Delta, Egypt","volume":"8","author":"Periago","year":"2008","journal-title":"Infect. Genet. Evol."},{"issue":"2","key":"10.1016\/j.parint.2022.102555_bb0065","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1051\/parasite\/2004112153","article-title":"Comparison of humoral response in sheep to Fasciola hepatica and Fasciola gigantica experimental infection","volume":"11","author":"Zhang","year":"2004","journal-title":"Parasite."},{"issue":"3","key":"10.1016\/j.parint.2022.102555_bb0070","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.exppara.2005.06.005","article-title":"Fasciola hepatica and Fasciola gigantica: comparison of cellular response to experimental infection in sheep","volume":"111","author":"Zhang","year":"2005","journal-title":"Exp. Parasitol."},{"issue":"5","key":"10.1016\/j.parint.2022.102555_bb0075","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1007\/s00436-005-1306-x","article-title":"Comparison of modulation of sheep, mouse and buffalo lymphocyte responses by Fasciola hepatica and Fasciola gigantica excretory-secretory products","volume":"95","author":"Zhang","year":"2005","journal-title":"Parasitol. Res."},{"issue":"1\u20132","key":"10.1016\/j.parint.2022.102555_bb0080","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.vetpar.2003.12.006","article-title":"Characterisation of Fasciola species from Mainland China by ITS-2 ribosomal DNA sequence","volume":"120","author":"Huang","year":"2004","journal-title":"Vet. Parasitol."},{"issue":"3","key":"10.1016\/j.parint.2022.102555_bb0085","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1007\/s00436-007-0512-0","article-title":"Sequence analysis of the first internal transcribed spacer of rDNA supports the existence of the intermediate Fasciola between F. hepatica and F. gigantica in mainland China","volume":"101","author":"Lin","year":"2007","journal-title":"Parasitol. Res."},{"issue":"1","key":"10.1016\/j.parint.2022.102555_bb0090","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1186\/1756-3305-7-150","article-title":"Complete mitochondrial genomes of the \u2018intermediate form\u2019 of Fasciola and Fasciola gigantica, and their comparison with F. hepatica","volume":"7","author":"Liu","year":"2014","journal-title":"Parasit. Vectors"},{"issue":"3","key":"10.1016\/j.parint.2022.102555_bb0095","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1007\/s00436-009-1459-0","article-title":"Molecular characterization of Fasciola hepatica, Fasciola gigantica, and aspermic Fasciola sp. in China based on nuclear and mitochondrial DNA","volume":"105","author":"Peng","year":"2009","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.parint.2022.102555_bb0100","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.exppara.2014.09.006","article-title":"Identification of Fasciola species based on mitochondrial and nuclear DNA reveals the co-existence of intermediate Fasciola and Fasciola gigantica in Thailand","volume":"146","author":"Wannasan","year":"2014","journal-title":"Exp. Parasitol."},{"issue":"4","key":"10.1016\/j.parint.2022.102555_bb0105","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1017\/S0022149X13000369","article-title":"Phenotypes of intermediate Fasciolas of Fasciola hepatica and Fasciola gigantica in buffaloes from Central Punjab, Pakistan","volume":"88","author":"Afshan","year":"2017","journal-title":"J. Helminthol."},{"issue":"1\u20133","key":"10.1016\/j.parint.2022.102555_bb0110","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.vetpar.2011.01.059","article-title":"A TaqMan real-time PCR-based assay for the identification of Fasciola spp","volume":"179","author":"Alasaad","year":"2011","journal-title":"Vet. Parasitol."},{"issue":"4","key":"10.1016\/j.parint.2022.102555_bb0115","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.biotechadv.2008.03.003","article-title":"Toward practical, DNA-based diagnostic methods for parasitic nematodes of livestock-bionomic and biotechnological implications","volume":"26","author":"Gasser","year":"2008","journal-title":"Biotechnol. Adv."},{"issue":"2","key":"10.1016\/j.parint.2022.102555_bb0120","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.gene.2014.03.017","article-title":"Comparative analyses of the complete mitochondrial genomes of the two ruminant hookworms Bunostomum trigonocephalum and Bunostomum phlebotomum","volume":"541","author":"Gao","year":"2014","journal-title":"Gene."},{"issue":"6","key":"10.1016\/j.parint.2022.102555_bb0125","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.parint.2022.102555_bb0130","first-page":"71","article-title":"DNASTAR\u2019s Lasergene sequence analysis software","volume":"132","author":"Burland","year":"2000","journal-title":"Methods Mol. Biol."},{"issue":"pt5","key":"10.1016\/j.parint.2022.102555_bb0135","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1017\/S0031182005008292","article-title":"Genetic characterization of parthenogenic Fasciola sp. in Japan on the basis of the sequences of ribosomal and mitochondrial DNA","volume":"131","author":"Itagaki","year":"2005","journal-title":"Parasitology."},{"issue":"11","key":"10.1016\/j.parint.2022.102555_bb0140","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1292\/jvms.67.1115","article-title":"Molecular characterization of parthenogenic Fasciola sp. in Korea on the basis of DNA sequences of ribosomal ITS1 and mitochondrial NDI gene","volume":"67","author":"Itagaki","year":"2005","journal-title":"J. Vet. Med. Sci."},{"issue":"10","key":"10.1016\/j.parint.2022.102555_bb0145","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1017\/S0031182011000965","article-title":"Hybridization experiments indicate incomplete reproductive isolating mechanism between Fasciola hepatica and Fasciola gigantica","volume":"138","author":"Itagaki","year":"2011","journal-title":"Parasitology."},{"issue":"1","key":"10.1016\/j.parint.2022.102555_bb0150","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1292\/jvms.17-0406","article-title":"Hybrid origin of asian aspermic fasciola flukes is confirmed by analyzing two single-copy genes, pepck and pold","volume":"80","author":"Hayashi","year":"2018","journal-title":"J. Vet. Med. Sci."}],"container-title":["Parasitology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1383576922000198?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1383576922000198?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T10:34:24Z","timestamp":1761302064000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1383576922000198"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6]]},"references-count":30,"alternative-id":["S1383576922000198"],"URL":"https:\/\/doi.org\/10.1016\/j.parint.2022.102555","relation":{},"ISSN":["1383-5769"],"issn-type":[{"type":"print","value":"1383-5769"}],"subject":[],"published":{"date-parts":[[2022,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Identification of new polymorphic positions in rDNA sequences of the \u201cintermediate\u201d Fasciola forms","name":"articletitle","label":"Article Title"},{"value":"Parasitology International","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.parint.2022.102555","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"102555"}}