{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T17:21:36Z","timestamp":1789320096924,"version":"build-2803163510"},"reference-count":38,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","pathologyjournal.rcpa.edu.au","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Pathology"],"published-print":{"date-parts":[[2023,6]]},"DOI":"10.1016\/j.pathol.2022.11.012","type":"journal-article","created":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T02:17:10Z","timestamp":1676341030000},"page":"478-485","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Improving sarcoma classification by using RNA hybridisation capture sequencing in sarcomas of uncertain histogenesis of young individuals"],"prefix":"10.1016","volume":"55","author":[{"given":"Ana Cristina","family":"Vargas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3426-9350","authenticated-orcid":false,"given":"Erin E.","family":"Heyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison L.","family":"Cheah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona","family":"Bonar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6468-2368","authenticated-orcid":false,"given":"Fiona M.","family":"Maclean","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9447-1967","authenticated-orcid":false,"given":"Anthony J.","family":"Gill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5216-8815","authenticated-orcid":false,"given":"James","family":"Blackburn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.pathol.2022.11.012_bib1","series-title":"WHO Classification of Tumours Editorial Board. World Health Organ Classification Tumours. Soft Tissue and Bone Tumours","year":"2020"},{"key":"10.1016\/j.pathol.2022.11.012_bib2","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1038\/nrc2091","article-title":"The impact of translocations and gene fusions on cancer causation","volume":"7","author":"Mitelman","year":"2007","journal-title":"Nat Rev Cancer"},{"key":"10.1016\/j.pathol.2022.11.012_bib3","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1002\/gcc.22745","article-title":"Most gene fusions in cancer are stochastic events","volume":"58","author":"Johansson","year":"2019","journal-title":"Genes Chromosomes Cancer"},{"key":"10.1016\/j.pathol.2022.11.012_bib4","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1007\/s11912-017-0641-2","article-title":"The role of next-generation sequencing in sarcomas: evolution from light microscope to molecular microscope","volume":"19","author":"Groisberg","year":"2017","journal-title":"Curr Oncol Rep"},{"key":"10.1016\/j.pathol.2022.11.012_bib5","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1007\/s11864-018-0589-y","article-title":"Is there value in molecular profiling of soft-tissue sarcoma?","volume":"19","author":"Italiano","year":"2018","journal-title":"Curr Treat Options Oncol"},{"key":"10.1016\/j.pathol.2022.11.012_bib6","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/j.jmoldx.2018.05.007","article-title":"Molecular analysis of gene fusions in bone and soft tissue tumors by anchored multiplex PCR-based targeted next-generation sequencing","volume":"20","author":"Lam","year":"2018","journal-title":"J\u00a0Mol Diagn"},{"key":"10.1016\/j.pathol.2022.11.012_bib7","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1002\/gcc.22690","article-title":"Targeted RNA sequencing: a routine ancillary technique in the diagnosis of bone and soft tissue neoplasms","volume":"58","author":"Dickson","year":"2019","journal-title":"Genes Chromosomes Cancer"},{"key":"10.1016\/j.pathol.2022.11.012_bib8","doi-asserted-by":"crossref","first-page":"5436","DOI":"10.3390\/cancers13215436","article-title":"Clinical value of NGS genomic studies for clinical management of pediatric and young adult bone sarcomas","volume":"13","author":"Gutierrez-Jimeno","year":"2021","journal-title":"Cancers (Basel)"},{"key":"10.1016\/j.pathol.2022.11.012_bib9","doi-asserted-by":"crossref","DOI":"10.1097\/PAS.0000000000001552","article-title":"The 2020 WHO Classification: what's new in soft tissue tumor pathology?","volume":"45","author":"Kallen","year":"2021","journal-title":"Am J Surg Pathol"},{"key":"10.1016\/j.pathol.2022.11.012_bib10","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1111\/his.14460","article-title":"'I can't keep up!': an update on advances in soft tissue pathology occurring after the publication of the 2020 World Health Organization classification of soft tissue and bone tumours","volume":"80","author":"Folpe","year":"2022","journal-title":"Histopathology"},{"key":"10.1016\/j.pathol.2022.11.012_bib11","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1038\/s41467-019-09374-9","article-title":"Diagnosis of fusion genes using targeted RNA sequencing","volume":"10","author":"Heyer","year":"2019","journal-title":"Nat Commun"},{"key":"10.1016\/j.pathol.2022.11.012_bib12","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.1016\/j.humpath.2014.04.012","article-title":"Molecular profiling of soft tissue sarcomas using next-generation sequencing: a pilot study toward precision therapeutics","volume":"45","author":"Jour","year":"2014","journal-title":"Hum Pathol"},{"key":"10.1016\/j.pathol.2022.11.012_bib13","doi-asserted-by":"crossref","first-page":"1398","DOI":"10.1001\/jamaoncol.2018.0723","article-title":"Targetable alterations in adult patients with soft-tissue sarcomas: insights for personalized therapy","volume":"4","author":"Lucchesi","year":"2018","journal-title":"JAMA Oncol"},{"key":"10.1016\/j.pathol.2022.11.012_bib14","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1016\/S1470-2045(15)00583-5","article-title":"Clinical effect of molecular methods in sarcoma diagnosis (GENSARC): a prospective, multicentre, observational study","volume":"17","author":"Italiano","year":"2016","journal-title":"Lancet Oncol"},{"key":"10.1016\/j.pathol.2022.11.012_bib15","doi-asserted-by":"crossref","first-page":"3410","DOI":"10.3390\/cancers13143410","article-title":"Hedgehog\/GLI signaling pathway: transduction, regulation, and implications for disease","volume":"13","author":"Sigafoos","year":"2021","journal-title":"Cancers (Basel)"},{"key":"10.1016\/j.pathol.2022.11.012_bib16","doi-asserted-by":"crossref","first-page":"471","DOI":"10.3389\/fgene.2018.00471","article-title":"Long non-coding RNA NEAT1: a novel target for diagnosis and therapy in human tumors","volume":"9","author":"Dong","year":"2018","journal-title":"Front Genet"},{"key":"10.1016\/j.pathol.2022.11.012_bib17","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1002\/1878-0261.12404","article-title":"Involvement of the long noncoding RNA NEAT1 in carcinogenesis","volume":"13","author":"Klec","year":"2019","journal-title":"Mol Oncol"},{"key":"10.1016\/j.pathol.2022.11.012_bib18","doi-asserted-by":"crossref","first-page":"3428","DOI":"10.7150\/ijbs.62728","article-title":"NEAT1 as a competing endogenous RNA in tumorigenesis of various cancers: role, mechanism and therapeutic potential","volume":"17","author":"Li","year":"2021","journal-title":"Int J Biol Sci"},{"key":"10.1016\/j.pathol.2022.11.012_bib19","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/1471-2164-8-39","article-title":"A\u00a0screen for nuclear transcripts identifies two linked noncoding RNAs associated with SC35 splicing domains","volume":"8","author":"Hutchinson","year":"2007","journal-title":"BMC Genomics"},{"key":"10.1016\/j.pathol.2022.11.012_bib20","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.1038\/modpathol.2017.68","article-title":"Gastroblastoma harbors a recurrent somatic MALAT1-GLI1 fusion gene","volume":"30","author":"Graham","year":"2017","journal-title":"Mod Pathol"},{"key":"10.1016\/j.pathol.2022.11.012_bib21","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1002\/path.4730","article-title":"Recurrent MALAT1-GLI1 oncogenic fusion and GLI1 up-regulation define a subset of plexiform fibromyxoma","volume":"239","author":"Spans","year":"2016","journal-title":"J\u00a0Pathol"},{"key":"10.1016\/j.pathol.2022.11.012_bib22","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1097\/PAS.0000000000001010","article-title":"A\u00a0distinct malignant epithelioid neoplasm with GLI1 gene rearrangements, frequent s100 protein expression, and metastatic potential: expanding the spectrum of pathologic entities with ACTB\/MALAT1\/PTCH1-GLI1 fusions","volume":"42","author":"Antonescu","year":"2018","journal-title":"Am J Surg Pathol"},{"key":"10.1016\/j.pathol.2022.11.012_bib23","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1016\/S0002-9440(10)63723-6","article-title":"Activation of the GLI oncogene through fusion with the beta-actin gene (ACTB) in a group of distinctive pericytic neoplasms: pericytoma with t(7;12)","volume":"164","author":"Dahlen","year":"2004","journal-title":"Am J Pathol"},{"key":"10.1016\/j.pathol.2022.11.012_bib24","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1111\/his.14280","article-title":"A\u00a0case of pericytic neoplasm in the shoulder with a novel DERA-GLI1 gene fusion","volume":"78","author":"Nitta","year":"2021","journal-title":"Histopathology"},{"key":"10.1016\/j.pathol.2022.11.012_bib25","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1097\/PAS.0000000000001439","article-title":"Head and neck mesenchymal neoplasms with GLI1 gene alterations: a pathologic entity with distinct histologic features and potential for distant metastasis","volume":"44","author":"Xu","year":"2020","journal-title":"Am J Surg Pathol"},{"key":"10.1016\/j.pathol.2022.11.012_bib26","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1038\/s41379-019-0293-x","article-title":"GLI1-amplifications expand the spectrum of soft tissue neoplasms defined by GLI1 gene fusions","volume":"32","author":"Agaram","year":"2019","journal-title":"Mod Pathol"},{"key":"10.1016\/j.pathol.2022.11.012_bib27","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1177\/1066896919900548","article-title":"A\u00a0malignant neoplasm from the jejunum with a MALAT1-GLI1 fusion and 26-year survival history","volume":"28","author":"Prall","year":"2020","journal-title":"Int J Surg Pathol"},{"key":"10.1016\/j.pathol.2022.11.012_bib28","doi-asserted-by":"crossref","first-page":"2259","DOI":"10.1158\/1078-0432.CCR-08-2184","article-title":"The NFATc2 gene is involved in a novel cloned translocation in a Ewing sarcoma variant that couples its function in immunology to oncology","volume":"15","author":"Szuhai","year":"2009","journal-title":"Clin Cancer Res"},{"key":"10.1016\/j.pathol.2022.11.012_bib29","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1097\/PAS.0000000000001260","article-title":"EWSR1-NFATC2 Translocation-associated sarcoma clinicopathologic findings in a rare aggressive primary bone or soft tissue tumor","volume":"43","author":"Wang","year":"2019","journal-title":"Am J Surg Pathol"},{"key":"10.1016\/j.pathol.2022.11.012_bib30","doi-asserted-by":"crossref","first-page":"1930","DOI":"10.1038\/s41379-020-0542-z","article-title":"NFATc2-rearranged sarcomas: clinicopathologic, molecular, and cytogenetic study of 7 cases with evidence of AGGRECAN as a novel diagnostic marker","volume":"33","author":"Perret","year":"2020","journal-title":"Mod Pathol"},{"key":"10.1016\/j.pathol.2022.11.012_bib31","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1038\/s41698-021-00177-0","article-title":"Multiscale-omic assessment of EWSR1-NFATc2 fusion positive sarcomas identifies the mTOR pathway as a potential therapeutic target","volume":"5","author":"Seligson","year":"2021","journal-title":"NPJ Precis Oncol"},{"key":"10.1016\/j.pathol.2022.11.012_bib32","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.1097\/PAS.0000000000001748","article-title":"Expanding the spectrum of EWSR1-NFATC2-rearranged benign tumors: a common genomic abnormality in vascular malformation\/hemangioma and simple bone cyst","volume":"45","author":"Ong","year":"2021","journal-title":"Am J Surg Pathol"},{"key":"10.1016\/j.pathol.2022.11.012_bib33","doi-asserted-by":"crossref","first-page":"1623","DOI":"10.1097\/PAS.0000000000001584","article-title":"FUS-NFATC2 or EWSR1-NFATC2 fusions are present in a large proportion of simple bone cysts","volume":"44","author":"Pizem","year":"2020","journal-title":"Am J Surg Pathol"},{"key":"10.1016\/j.pathol.2022.11.012_bib34","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1111\/his.14314","article-title":"Identification of EWSR1-NFATC2 fusion in simple bone cysts","volume":"78","author":"Hung","year":"2021","journal-title":"Histopathology"},{"key":"10.1016\/j.pathol.2022.11.012_bib35","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1097\/CCO.0000000000000741","article-title":"Next-generation sequencing for the management of sarcomas with no known driver mutations","volume":"33","author":"Vyse","year":"2021","journal-title":"Curr Opin Oncol"},{"key":"10.1016\/j.pathol.2022.11.012_bib36","doi-asserted-by":"crossref","first-page":"1520","DOI":"10.5858\/arpa.2013-0691-RA","article-title":"A\u00a0review of preanalytical factors affecting molecular, protein, and morphological analysis of formalin-fixed, paraffin-embedded (FFPE) tissue: how well do you know your FFPE specimen?","volume":"138","author":"Bass","year":"2014","journal-title":"Arch Pathol Lab Med"},{"key":"10.1016\/j.pathol.2022.11.012_bib37","doi-asserted-by":"crossref","first-page":"6980","DOI":"10.1038\/s41598-019-43282-8","article-title":"Deleterious effects of formalin-fixation and delays to fixation on RNA and miRNA-Seq profiles","volume":"9","author":"Jones","year":"2019","journal-title":"Sci Rep"},{"key":"10.1016\/j.pathol.2022.11.012_bib38","series-title":"Guideline: fixation of tissues","year":"2020"}],"container-title":["Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0031302523000521?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0031302523000521?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T16:30:25Z","timestamp":1758472225000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0031302523000521"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6]]},"references-count":38,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["S0031302523000521"],"URL":"https:\/\/doi.org\/10.1016\/j.pathol.2022.11.012","relation":{},"ISSN":["0031-3025"],"issn-type":[{"value":"0031-3025","type":"print"}],"subject":[],"published":{"date-parts":[[2023,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Improving sarcoma classification by using RNA hybridisation capture sequencing in sarcomas of uncertain histogenesis of young individuals","name":"articletitle","label":"Article Title"},{"value":"Pathology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.pathol.2022.11.012","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Royal College of Pathologists of Australasia. Published by Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}