{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T20:41:59Z","timestamp":1747168919041,"version":"3.40.5"},"reference-count":25,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,7,14]],"date-time":"2024-07-14T00:00:00Z","timestamp":1720915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["clinicalkey.fr","clinicalkey.jp","clinicalkey.com.au","clinicalkey.es","clinicalkey.com","pathologyjournal.rcpa.edu.au","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Pathology"],"published-print":{"date-parts":[[2024,10]]},"DOI":"10.1016\/j.pathol.2024.04.005","type":"journal-article","created":{"date-parts":[[2024,6,20]],"date-time":"2024-06-20T16:59:01Z","timestamp":1718902741000},"page":"889-896","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["A novel molecular assay conducted on the BD Max system to facilitate reflex testing for vanA and vanB in clinical isolates of enterococci"],"prefix":"10.1016","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-7609-7244","authenticated-orcid":false,"given":"Christopher J.","family":"McIver","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2985-4280","authenticated-orcid":false,"given":"Ryanbi","family":"Pratama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gismin","family":"Jose","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dale","family":"Caagbay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leanne","family":"Montgomery","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5088-6989","authenticated-orcid":false,"given":"Narinder","family":"Bhardwaj","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1175-6123","authenticated-orcid":false,"given":"Chinmoy","family":"Mukerjee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9390-2844","authenticated-orcid":false,"given":"Peter C.","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6197-8414","authenticated-orcid":false,"given":"Robert","family":"Stevens","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"year":"2018","series-title":"Australian Passive Antimicrobial Resistance Surveillance. First Report: Multi-resistant Organisms","key":"10.1016\/j.pathol.2024.04.005_bib1"},{"key":"10.1016\/j.pathol.2024.04.005_bib2","article-title":"Disease burden, associated mortality and economic impact of antimicrobial resistant infections in Australia","volume":"27","author":"Wosniak","year":"2022","journal-title":"Lancet Reg Health West Pac"},{"key":"10.1016\/j.pathol.2024.04.005_bib3","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1093\/jac\/dkab379","article-title":"A\u00a0vanA vancomycin-resistant Enterococcus faecium ST80 outbreak resulting from a single importation event","volume":"77","author":"Pratama","year":"2022","journal-title":"J\u00a0Antimicrob Chemother"},{"key":"10.1016\/j.pathol.2024.04.005_bib4","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1128\/AAC.49.1.77-81.2005","article-title":"Comparison of three PCR primer sets for identification of vanB gene carriage in feces and correlation with carriage of vancomycin-resistant enterococci: interference by vanB-containing anaerobic bacilli","volume":"49","author":"Ballard","year":"2005","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.pathol.2024.04.005_bib5","doi-asserted-by":"crossref","first-page":"1688","DOI":"10.1128\/AAC.49.5.1688-1694.2005","article-title":"Molecular characterization of vanB elements in naturally occurring gut anaerobes","volume":"49","author":"Ballard","year":"2005","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.pathol.2024.04.005_bib6","doi-asserted-by":"crossref","first-page":"294","DOI":"10.3390\/bios13020294","article-title":"A\u00a0review of detection methods for vancomycin-resistant Enterococci (VRE) genes: from conventional approaches to potentially electrochemical DNA biosensors","volume":"13","author":"Zakaria","year":"2023","journal-title":"Biosens"},{"key":"10.1016\/j.pathol.2024.04.005_bib7","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0009869","article-title":"Evaluation of GeneXpert vanA\/vanB in the early diagnosis of vancomycin-resistant enterococci infection","volume":"15","author":"Li","year":"2021","journal-title":"PLoS Negl Trop Dis"},{"key":"10.1016\/j.pathol.2024.04.005_bib8","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.00543-21","article-title":"Usefulness of BioFire FilmArray BCID2 for blood culture processing in clinical practice","volume":"59","author":"Berinson","year":"2021","journal-title":"J\u00a0Clin Microbiol"},{"key":"10.1016\/j.pathol.2024.04.005_bib9","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1586\/14737159.2016.1134325","article-title":"Sample-to-result molecular infectious disease assays: clinical implications, limitations and potential","volume":"16","author":"Beal","year":"2016","journal-title":"Expert Rev Mol Diagn"},{"key":"10.1016\/j.pathol.2024.04.005_bib10","doi-asserted-by":"crossref","first-page":"2597","DOI":"10.1128\/JB.01695-07","article-title":"The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse","volume":"190","author":"Durfee","year":"2008","journal-title":"J\u00a0Bacteriol"},{"key":"10.1016\/j.pathol.2024.04.005_bib11","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1016\/j.jmii.2019.02.002","article-title":"Real-time PCR for the rapid detection of vanA, vanB, and vanM genes","volume":"53","author":"He","year":"2020","journal-title":"J\u00a0Microbiol Immunol Infect"},{"key":"10.1016\/j.pathol.2024.04.005_bib12","first-page":"117","article-title":"Heat treatment of bacteria: a simple method of DNA extraction for molecular techniques","volume":"41","author":"Dashti","year":"2009","journal-title":"Kuwait Med J"},{"key":"10.1016\/j.pathol.2024.04.005_bib13","series-title":"Microbiological methods","first-page":"134","author":"Collins","year":"1984"},{"key":"10.1016\/j.pathol.2024.04.005_bib14","series-title":"PCR Troubleshooting and Optimization","first-page":"139","article-title":"Quantitative PCR data analysis \u2013 unlocking the secret to successful results","author":"Hellemans","year":"2011"},{"key":"10.1016\/j.pathol.2024.04.005_bib15","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/B978-0-12-814515-9.00053-9","article-title":"Validating real-time polymerase chain reaction (PCR) assays","author":"Smith","year":"2021","journal-title":"Encyclopedia Virol"},{"key":"10.1016\/j.pathol.2024.04.005_bib16","doi-asserted-by":"crossref","first-page":"2269","DOI":"10.2147\/IDR.S177886","article-title":"Molecular detection of vanA and vanB genes among vancomycin-resistant enterococci in ICU-hospitalized patients in southwest of Iran","volume":"11","author":"Moosavian","year":"2018","journal-title":"Infect Drug Resist"},{"key":"10.1016\/j.pathol.2024.04.005_bib17","series-title":"Real-time PCR: Current Technology and Applications","first-page":"85","article-title":"Performing real-time PCR","author":"Edwards","year":"2009"},{"key":"10.1016\/j.pathol.2024.04.005_bib18","first-page":"963","article-title":"Transferable, plasmid-mediated vanB-type glycopeptide resistance in Enterococcus faecium. Antimicrob Agents Chemother","volume":"42","author":"Rice","year":"1998"},{"key":"10.1016\/j.pathol.2024.04.005_bib19","first-page":"2383","article-title":"Large conjugative vanA plasmids in vancomycin-resistant Enterococcus faecium. J Clin Microbiol","volume":"37","author":"Werner","year":"1999"},{"key":"10.1016\/j.pathol.2024.04.005_bib20","doi-asserted-by":"crossref","first-page":"335","DOI":"10.5001\/omj.2014.90","article-title":"VanA and VanB positive vancomycin-resistant Staphylococcus aureus among clinical isolates in Shiraz, South of Iran","volume":"29","author":"Saadat","year":"2014","journal-title":"Oman Med J"},{"key":"10.1016\/j.pathol.2024.04.005_bib21","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1590\/0037-8682-0159-2017","article-title":"Occurrence of the vanA gene in Staphylococcus epidermidis from nasopharyngeal secretion of health-care workers, Recife, Brazil","volume":"51","author":"Neto","year":"2018","journal-title":"Rev Soc Bras Med Trop"},{"key":"10.1016\/j.pathol.2024.04.005_bib22","doi-asserted-by":"crossref","first-page":"108","DOI":"10.3389\/fmicb.2017.00108","article-title":"A\u00a0basic guide to real time PCR in microbial diagnostics: definitions, parameters, and everything","volume":"8","author":"Kralik","year":"2017","journal-title":"Front Microbiol"},{"key":"10.1016\/j.pathol.2024.04.005_bib23","series-title":"PCR Troubleshooting and Optimization","first-page":"79","article-title":"Obtaining maximum PCR sensitivity and specificity","author":"Cameron","year":"2011"},{"issue":"Suppl 1","key":"10.1016\/j.pathol.2024.04.005_bib24","first-page":"S117","article-title":"A\u00a0novel method for harvesting bacteria from BacT\/ALERT\u00ae blood culture system to enable PCR testing","volume":"49","author":"McIver","year":"2017","journal-title":"Pathology"},{"key":"10.1016\/j.pathol.2024.04.005_bib25","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0009869","article-title":"Evaluation of GeneXpert vanA\/vanB in the early diagnosis of vancomycin-resistant enterococci infection","volume":"15","author":"Li","year":"2021","journal-title":"PLoS Negl Trop Dis"}],"container-title":["Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0031302524001442?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0031302524001442?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,3,15]],"date-time":"2025-03-15T13:21:22Z","timestamp":1742044882000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0031302524001442"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10]]},"references-count":25,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["S0031302524001442"],"URL":"https:\/\/doi.org\/10.1016\/j.pathol.2024.04.005","relation":{},"ISSN":["0031-3025"],"issn-type":[{"type":"print","value":"0031-3025"}],"subject":[],"published":{"date-parts":[[2024,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A novel molecular assay conducted on the BD Max system to facilitate reflex testing for vanA and vanB in clinical isolates of enterococci","name":"articletitle","label":"Article Title"},{"value":"Pathology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.pathol.2024.04.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier B.V. on behalf of Royal College of Pathologists of Australasia.","name":"copyright","label":"Copyright"}]}}