{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T16:02:52Z","timestamp":1775232172007,"version":"3.50.1"},"reference-count":47,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2006,11,1]],"date-time":"2006-11-01T00:00:00Z","timestamp":1162339200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2006,11,1]],"date-time":"2006-11-01T00:00:00Z","timestamp":1162339200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,1,11]],"date-time":"2021-01-11T00:00:00Z","timestamp":1610323200000},"content-version":"vor","delay-in-days":5185,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Progress in Biophysics and Molecular Biology"],"published-print":{"date-parts":[[2006,11]]},"DOI":"10.1016\/j.pbiomolbio.2006.05.001","type":"journal-article","created":{"date-parts":[[2006,7,6]],"date-time":"2006-07-06T18:52:09Z","timestamp":1152211929000},"page":"333-370","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":20,"title":["Critical review of the methods used to measure the apparent dissociation constant and ligand purity in Ca2+ and Mg2+ buffer solutions"],"prefix":"10.1016","volume":"92","author":[{"given":"John A.S.","family":"McGuigan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James W.","family":"Kay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hugh Y.","family":"Elder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib1","series-title":"Molecular Biology of the Cell","author":"Alberts","year":"2002"},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib2","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1126\/science.841325","article-title":"Aequorin luminescence: relation of light emission to calcium concentration\u2014a calcium-independent component","volume":"195","author":"Allen","year":"1977","journal-title":"Science"},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib3","series-title":"Ion-Selective Micro-Electrodes. Principles, Design and Application","author":"Ammann","year":"1986"},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib4","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1080\/10408348108085499","article-title":"The modern meaning of pH","volume":"10","author":"Bates","year":"1981","journal-title":"CRC Critical Reviews in Analytical Chemistry"},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib5","doi-asserted-by":"crossref","first-page":"C404","DOI":"10.1152\/ajpcell.1982.242.5.C404","article-title":"A simple method for the accurate determination of free [Ca] in Ca\u2013EGTA solutions","volume":"242","author":"Bers","year":"1982","journal-title":"Am. J. Physiol."},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib6","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1085\/jgp.49.1.131","article-title":"Calcium binding by particle-free supernatants of homogenates of skeletal muscle","volume":"49","author":"Briggs","year":"1965","journal-title":"J. Gen. Physiol."},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib7","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1113\/expphysiol.1993.sp003682","article-title":"The regulation of intracellular Mg2+ in guinea-pig heart, studied with Mg2+-selective microelectrodes and fluorochromes","volume":"78","author":"Buri","year":"1993","journal-title":"Exp. Physiol."},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib8","series-title":"Ringer Solutions and Physiological Salines","author":"Burton","year":"1975"},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib9","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1113\/jphysiol.1983.sp014891","article-title":"Sodium\/calcium exchange in mammalian ventricular muscle: A study with sodium-sensitive micro-electrodes","volume":"343","author":"Chapman","year":"1983","journal-title":"J. Physiol."},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib10","series-title":"Ion Association","author":"Davies","year":"1962"},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib11","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1093\/oxfordjournals.jbchem.a127439","article-title":"Calcium binding activity of vesicular relaxing factor","volume":"50","author":"Ebashi","year":"1961","journal-title":"J. Biochem."},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib12","unstructured":"Ebashi, S., Endo, M., 1962. Unpublished, quoted by Ogawa, Y. 1968."},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib13","first-page":"463","article-title":"Calculator programs for computing the composition of solutions containing multiple metals and ligands used for experiments on skinned muscle cells","volume":"75","author":"Fabiato","year":"1979","journal-title":"J. Physiol. (Paris)"},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib14","series-title":"Ion-Selective Electrodes for Biological Systems","author":"Fry","year":"2001"},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib15","series-title":"Calcium-Activated Tension of Skinned Muscle Fibres: Dependence on MgATP Concentration","author":"Godt","year":"1971"},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib16","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1085\/jgp.63.6.722","article-title":"Calcium-activated tension of skinned muscle fibres of the frog. Dependence on magnesium adenosine triphosphate concentration","volume":"63","author":"Godt","year":"1974","journal-title":"J. Gen. Physiol."},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib17","doi-asserted-by":"crossref","first-page":"905","DOI":"10.2741\/1585","article-title":"Use of Mg2+ and Ca2+ macroelectrodes to measure binding in extracellular-like physiological solutions","volume":"10","author":"G\u00fcnzel","year":"2005","journal-title":"Front. Biosci."},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib18","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1093\/oxfordjournals.jbchem.a132868","article-title":"Re-examination of the apparent binding constant of ethylene glycol bis(\u03b2-aminoethyl ether)-N,N,N\u2032N\u2032-tetraacetic acid with calcium around neutral pH","volume":"87","author":"Harafuji","year":"1980","journal-title":"J. Biochem."},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib19","doi-asserted-by":"crossref","first-page":"C1250","DOI":"10.1152\/ajpcell.1989.256.6.C1250","article-title":"Correction of proton and Ca association constants of EGTA for temperature and I","volume":"256","author":"Harrison","year":"1989","journal-title":"Am. J. Physiol."},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib20","article-title":"Automatic determination of ligand purity and apparent equilibrium constant in divalent cation buffer solutions and apparent equilibrium constant in physiological solutions","author":"Kay","year":"2004","journal-title":"J. Physiol."},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib21","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1113\/jphysiol.1956.sp005652","article-title":"The intracellular calcium contents of some invertebrate nerves","volume":"134","author":"Keynes","year":"1956","journal-title":"J. Physiol."},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib22","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/S0143-4160(96)90028-7","article-title":"Some commonly overlooked properties of calcium buffer systems. A simple method for detecting and correcting stoichiometric imbalance in CaEGTA stock solutions","volume":"20","author":"Klabusay","year":"1996","journal-title":"Cell Calcium"},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib23","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1113\/expphysiol.1997.sp004039","article-title":"Calibration of Mg2+-selective macroelectrodes down to 1\u03bcmoll\u22121 in intracellular and Ca2+-containing extracellular solutions","volume":"82","author":"L\u00fcthi","year":"1997","journal-title":"Exp. Physiol."},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib24","first-page":"231","article-title":"Mg\u2013ATP binding: its modification by spermine, the relevance to cytosolic Mg2+ buffering, changes in the intracellular ionised Mg2+ concentration and the estimation of Mg2+ by 31P-NMR","volume":"84","author":"L\u00fcthi","year":"1999","journal-title":"Exp. Physiol."},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib25","series-title":"Critical Stability Constants 1; Amino Acids","author":"Martell","year":"1974"},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib26","series-title":"Critical Stability Constants 2: Amines","author":"Martell","year":"1974"},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib27","series-title":"Critical Stability Constants 3; Other Organic Ligands","author":"Martell","year":"1977"},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib28","series-title":"Use of ion Selective Microelectrodes to Measure Intracellular Free Mg2+. Mg2+ and Excitable Membranes","first-page":"1","author":"McGuigan","year":"1991"},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib29","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.1139\/y91-257","article-title":"Calcium buffer solutions and how to make them; a do it yourself guide","volume":"69","author":"McGuigan","year":"1991","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib30","series-title":"Magnesium and the Cell","first-page":"91","article-title":"Some theoretical and practical aspects of the measurement of the intracellular free magnesium concentration in heart muscle: consideration of its regulation and modulation","author":"McGuigan","year":"1993"},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib31","first-page":"5","article-title":"Magnesium homeostasis in heart: a critical reappraisal","volume":"5","author":"McGuigan","year":"2002","journal-title":"J. Clin. Basic Cardiol."},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib32","unstructured":"McGuigan, J.A.S., Kay, J.W. Elder, H.Y., 2004. Calcium and magnesium buffer solutions: the need for standardisation. J. Physiol. 557P, C89."},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib33","doi-asserted-by":"crossref","first-page":"C160","DOI":"10.1152\/ajpcell.1984.246.1.C160","article-title":"EGTA purity and the buffering of calcium ions in physiological solutions","volume":"246","author":"Miller","year":"1984","journal-title":"Am. J. Physiol."},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib34","series-title":"The Intracellular Control and Action of Calcium in Striated Muscle and the Forces Responsible for the Stability of the Myofilament Lattice","author":"Moisescu","year":"1973"},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib35","first-page":"R122","article-title":"A pH-metric method for the determination of the relative concentrations of calcium to EGTA","volume":"355","author":"Moisescu","year":"1975","journal-title":"Pfl\u00fcgers Archiv, Eur. J. Physiol."},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib36","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1523\/JNEUROSCI.4451-04.2005","article-title":"A cytosolic residue mediates Mg2+ block and regulates inward current amplitude of a transient receptor potential channel","volume":"25","author":"Obukhov","year":"2005","journal-title":"J. Neurosci."},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib37","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1093\/oxfordjournals.jbchem.a128887","article-title":"The apparent binding constant of glycoletherdiaminetetraacetic acid for calcium at neutral pH","volume":"64","author":"Ogawa","year":"1968","journal-title":"J. Biochem."},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib38","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/0143-4160(94)90060-4","article-title":"Apparent stability constants and purity of Ca-chelating agents evaluated using Ca-sensitive electrodes by the double-log optimization method","volume":"15","author":"Oiki","year":"1994","journal-title":"Cell Calcium"},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib39","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.ceca.2003.10.006","article-title":"Some precautions in using chelators to buffer metals in biological solutions","volume":"35","author":"Patton","year":"2004","journal-title":"Cell Calcium"},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib40","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1111\/j.1749-6632.1949.tb27297.x","article-title":"The attractions of proteins for small molecules and ions","volume":"51","author":"Scatchard","year":"1949","journal-title":"Ann. NY Acad. Sci."},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib41","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1021\/j150458a006","article-title":"The use of ion-exchangers for the determination of physical properties of substances, particularly radiotracers, in solution","volume":"52","author":"Schubert","year":"1948","journal-title":"J. Phys. Coll. Chem."},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib42","doi-asserted-by":"crossref","unstructured":"Smith, A., 1776. An enquiry into the nature and causes of the wealth of nations. vols. I & II. Printed for W. Strahan and T. Cadell, London, 510pp and 587pp.","DOI":"10.1093\/oseo\/instance.00043218"},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib43","series-title":"A Functional and Structural Study of Cardiac Muscle Subjected to Membrane Disruption Techniques","author":"Smith","year":"1983"},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib44","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/0304-4165(85)90011-X","article-title":"Potentiometric measurements of stoichiometric and apparent affinity constants of EGTA for protons and divalent ions including calcium","volume":"893","author":"Smith","year":"1985","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib45","doi-asserted-by":"crossref","first-page":"2396","DOI":"10.1021\/bi00552a018","article-title":"New calcium indicators and buffers with a high selectivity against magnesium and protons: design, synthesis and properties of prototype structures","volume":"19","author":"Tsien","year":"1980","journal-title":"Biochemistry"},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib46","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1016\/0005-2736(80)90205-9","article-title":"Neutral carrier ion-selective microelectrodes for the measurement of intracellular free calcium","volume":"839","author":"Tsien","year":"1980","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.pbiomolbio.2006.05.001_bib47","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1006\/abio.1997.2238","article-title":"Apparent Mg2+-adenosine 5-triphosphate dissociation constant measured with Mg2+ macroelectrodes under conditions pertinent to 31P NMR ionised magnesium determinations","volume":"251","author":"Zhang","year":"1997","journal-title":"Analy. Biochem."}],"container-title":["Progress in Biophysics and Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0079610706000484?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0079610706000484?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T21:39:35Z","timestamp":1758749975000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0079610706000484"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,11]]},"references-count":47,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,11]]}},"alternative-id":["S0079610706000484"],"URL":"https:\/\/doi.org\/10.1016\/j.pbiomolbio.2006.05.001","relation":{},"ISSN":["0079-6107"],"issn-type":[{"value":"0079-6107","type":"print"}],"subject":[],"published":{"date-parts":[[2006,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Critical review of the methods used to measure the apparent dissociation constant and ligand purity in Ca2+ and Mg2+ buffer solutions","name":"articletitle","label":"Article Title"},{"value":"Progress in Biophysics and Molecular Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.pbiomolbio.2006.05.001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2006 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}