{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:59:12Z","timestamp":1790719152784,"version":"4.1.0"},"reference-count":22,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2004,3,1]],"date-time":"2004-03-01T00:00:00Z","timestamp":1078099200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Expression and Purification"],"published-print":{"date-parts":[[2004,3]]},"DOI":"10.1016\/j.pep.2003.10.015","type":"journal-article","created":{"date-parts":[[2003,12,22]],"date-time":"2003-12-22T22:58:38Z","timestamp":1072133918000},"page":"8-16","source":"Crossref","is-referenced-by-count":17,"title":["Cloning, high-level expression, single-step purification, and binding activity of His6-tagged recombinant type B botulinum neurotoxin heavy chain transmembrane and binding domain"],"prefix":"10.1016","volume":"34","author":[{"given":"Yu","family":"Zhou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bal Ram","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.pep.2003.10.015_BIB1","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1038\/77900","article-title":"Intimate detail of the most poisonous poison","volume":"7","author":"Singh","year":"2000","journal-title":"Nat. Struct. Biol."},{"key":"10.1016\/j.pep.2003.10.015_BIB2","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1001\/jama.285.8.1059","article-title":"Botulinum toxin as a biological weapon: medical and public health management","volume":"285","author":"Arnon","year":"2001","journal-title":"J. Am. Med. Assoc."},{"key":"10.1016\/j.pep.2003.10.015_BIB3","doi-asserted-by":"crossref","first-page":"10498","DOI":"10.1016\/S0021-9258(17)34087-5","article-title":"Identification of protein receptor for Clostridium botulinum type B neurotoxin in rat brain synaptosomes","volume":"269","author":"Nishiki","year":"1994","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.pep.2003.10.015_BIB4","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/0014-5793(95)01471-3","article-title":"The high-affinity binding of Clostridium botulinum type B neurotoxin to synaptotagmin II associated with gangliosides GT1b\/GD1a","volume":"378","author":"Nishiki","year":"1996","journal-title":"FEBS Lett."},{"key":"10.1016\/j.pep.2003.10.015_BIB5","first-page":"215","article-title":"Isolation of synaptotagmin as a receptor for type A and E botulinum neurotoxin and analysis of their comparative binding using a new microtiter plate assay","volume":"7","author":"Li","year":"1998","journal-title":"J. Nat. Toxins"},{"key":"10.1016\/j.pep.2003.10.015_BIB6","doi-asserted-by":"crossref","first-page":"45","DOI":"10.3109\/15569549909036017","article-title":"Membrane channel activity and translocation of tetanus and botulinum neurotoxins","volume":"18","author":"Lebeda","year":"1999","journal-title":"J. Toxicol.-Toxin Rev."},{"key":"10.1016\/j.pep.2003.10.015_BIB7","doi-asserted-by":"crossref","first-page":"1515","DOI":"10.1016\/S0041-0101(01)00124-6","article-title":"Botulinum neurotoxin types B and E: purification, limited proteolysis by endoproteinase Glu-C and pepsin, and comparison of their identified cleaved sites relative to the three-dimensional structure of type A neurotoxin","volume":"39","author":"Prabakaran","year":"2001","journal-title":"Toxicon"},{"key":"10.1016\/j.pep.2003.10.015_BIB8","unstructured":"S. Swaminathan, S. Eswaramoorthy, Crystal structure of Clostridium botulinum neurotoxin serotype B, in: M.F. Brin, M. Hallett, J. Jankovic (Eds.) Scientific and Therapeutic Aspects of Botulinum Toxin, Lippincott Williams & Wilkins, Philadelphia, PA, 2002, pp. 29\u201339"},{"key":"10.1016\/j.pep.2003.10.015_BIB9","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1002\/pro.5560050221","article-title":"Topology prediction of membrane proteins","volume":"5","author":"Persson","year":"1996","journal-title":"Protein Sci."},{"key":"10.1016\/j.pep.2003.10.015_BIB10","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1016\/0041-0101(95)00072-T","article-title":"Expression of a large, nontoxic fragment of botulinum neurotoxin serotype A and its use as an immunogen","volume":"33","author":"LaPenotiere","year":"1995","journal-title":"Toxicon"},{"key":"10.1016\/j.pep.2003.10.015_BIB11","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1016\/S0041-0101(98)00146-9","article-title":"Development of recombinant vaccines for botulium neurotoxin","volume":"36","author":"Smith","year":"1998","journal-title":"Toxicon"},{"key":"10.1016\/j.pep.2003.10.015_BIB12","doi-asserted-by":"crossref","first-page":"2715","DOI":"10.1242\/jcs.112.16.2715","article-title":"Functional characterisation of tetanus and botulinum neurotoxins binding domains","volume":"112","author":"Lalli","year":"1999","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.pep.2003.10.015_BIB13","doi-asserted-by":"crossref","first-page":"2345","DOI":"10.1128\/AEM.58.8.2345-2354.1992","article-title":"Molecular cloning of the Clostridium botulinum structural gene encoding the type B and determination of its entire nucleotide sequence","volume":"58","author":"Whelan","year":"1992","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.pep.2003.10.015_BIB14","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1128\/IAI.14.3.680-686.1976","article-title":"Molecular forms of neurotoxins in proteolytic Clostridium botulinum type B cultures","volume":"14","author":"DasGupta","year":"1976","journal-title":"Infect. Immun."},{"key":"10.1016\/j.pep.2003.10.015_BIB15","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1006\/prep.1999.1138","article-title":"High-level expression, purification, and characterization of recombinant type A botulinum neurotoxin light chain","volume":"17","author":"Li","year":"1999","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.pep.2003.10.015_BIB16","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/0003-2697(80)90024-X","article-title":"An absolute method for protein determination based on difference in absorbance at 235 and 280nm","volume":"109","author":"Whitaker","year":"1980","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.pep.2003.10.015_BIB17","doi-asserted-by":"crossref","first-page":"2738","DOI":"10.1128\/IAI.63.7.2738-2742.1995","article-title":"Protective vaccination with a recombinant fragment of Clostridium botulinum neurotoxin serotype A expressed from a synthetic gene Escherichia coli","volume":"63","author":"Clayton","year":"1996","journal-title":"Infect. Immun."},{"key":"10.1016\/j.pep.2003.10.015_BIB18","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1006\/prep.1997.0772","article-title":"Recombinant expression and purification of the botulinum neurotoxin type A translocation domain","volume":"11","author":"Lacy","year":"1997","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.pep.2003.10.015_BIB19","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1023\/A:1020655701852","article-title":"In Vitro translation of type A Clostridium botulinum neurotoxin heavy chain and analysis of its binding to rat synaptosomes","volume":"18","author":"Li","year":"1999","journal-title":"J. Protein Chem."},{"key":"10.1016\/j.pep.2003.10.015_BIB20","first-page":"401","article-title":"Cloning and characterization of the upstream region of Clostridium botulinum type B neurotoxin gene","volume":"45","author":"Yang","year":"1998","journal-title":"Biochem. Mol. Biol. Int."},{"key":"10.1016\/j.pep.2003.10.015_BIB21","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1007\/BF01569055","article-title":"Nucleotide sequence of the gene coding for non-proteolytic Clostridium botulinum type B neurotoxin: comparison with other clostridial nerotoxins","volume":"28","author":"Hutson","year":"1994","journal-title":"Curr. Microbiol."},{"key":"10.1016\/j.pep.2003.10.015_BIB22","first-page":"19","article-title":"Sequence of the gene for Clostridium botulinum type B neurotoxin associated with infant botulism, expression of the C-terminal half of heavy chain and its binding activity","volume":"1625","author":"Ihara","year":"2003","journal-title":"Biochem. Biophys. Acta"}],"container-title":["Protein Expression and Purification"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592803003450?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592803003450?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,6,13]],"date-time":"2021-06-13T16:47:44Z","timestamp":1623602864000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046592803003450"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,3]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,3]]}},"alternative-id":["S1046592803003450"],"URL":"https:\/\/doi.org\/10.1016\/j.pep.2003.10.015","relation":{},"ISSN":["1046-5928"],"issn-type":[{"value":"1046-5928","type":"print"}],"subject":[],"published":{"date-parts":[[2004,3]]}}}