{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T10:14:40Z","timestamp":1790244880168,"version":"4.1.0"},"reference-count":31,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2005,3,1]],"date-time":"2005-03-01T00:00:00Z","timestamp":1109635200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Expression and Purification"],"published-print":{"date-parts":[[2005,3]]},"DOI":"10.1016\/j.pep.2004.11.001","type":"journal-article","created":{"date-parts":[[2004,12,16]],"date-time":"2004-12-16T00:22:51Z","timestamp":1103156571000},"page":"152-163","source":"Crossref","is-referenced-by-count":15,"title":["Yersinia pestis outer membrane type III secretion protein YscC: expression, purification, characterization, and induction of specific antiserum"],"prefix":"10.1016","volume":"40","author":[{"given":"Jeremy L.","family":"Goodin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald W.","family":"Raab","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert L.","family":"McKown","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George L.","family":"Coffman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bradford S.","family":"Powell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeff T.","family":"Enama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John A.","family":"Ligon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard P.","family":"Andrews","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.pep.2004.11.001_bib1","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1083\/jcb.200205077","article-title":"Yersinia type III secretion: send in the effectors","volume":"158","author":"Cornelis","year":"2002","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.pep.2004.11.001_bib2","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1078\/1438-4221-00153","article-title":"The Yersinia Ysc-Yop virulence apparatus","volume":"291","author":"Cornelis","year":"2002","journal-title":"Int. J. Med. Microbiol."},{"key":"10.1016\/j.pep.2004.11.001_bib3","doi-asserted-by":"crossref","first-page":"8778","DOI":"10.1073\/pnas.97.16.8778","article-title":"Molecular and cell biology aspects of plague","volume":"97","author":"Cornelis","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.pep.2004.11.001_bib4","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/S0966-842X(99)01665-0","article-title":"Type III machines of gram-negative bacteria: delivering the goods","volume":"8","author":"Cheng","year":"2000","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.pep.2004.11.001_bib5","doi-asserted-by":"crossref","first-page":"4645","DOI":"10.1128\/JB.186.14.4645-4654.2004","article-title":"Structure and electrophysiological properties of the YscC secretin from the type III secretion system of Yersinia enterocolitica","volume":"186","author":"Burghout","year":"2004","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.pep.2004.11.001_bib6","doi-asserted-by":"crossref","first-page":"3843","DOI":"10.1128\/jb.177.13.3843-3854.1995","article-title":"Mutations in yscC, yscD, and yscG prevent high-level expression and secretion of V antigen and Yops in Yersinia pestis","volume":"177","author":"Plano","year":"1995","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.pep.2004.11.001_bib7","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1128\/MMBR.62.4.1315-1352.1998","article-title":"The virulence plasmid of Yersinia, an antihost genome","volume":"62","author":"Cornelis","year":"1998","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"10.1016\/j.pep.2004.11.001_bib8","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1046\/j.1365-2958.1997.6141981.x","article-title":"The outer membrane component, YscC, of the Yop secretion machinery of Yersinia enterocolitica forms a ring-shaped multimeric complex","volume":"26","author":"Koster","year":"1997","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.pep.2004.11.001_bib9","unstructured":"National Research Council, Institute of Laboratory Animal Resources. Guide for the care and use of laboratory animals. U.S. Department of Health and Human Services Publication No. (NIH) 86-23. U.S. Department of Health and Human Services, Bethesda, 1986"},{"key":"10.1016\/j.pep.2004.11.001_bib10","first-page":"299","article-title":"Studies on the contribution of the F1 capsule-associated plasmid pFra to the virulence of Yersinia pestis","volume":"13","author":"Welkos","year":"1995","journal-title":"Contrib. Microbiol. Immunol."},{"key":"10.1016\/j.pep.2004.11.001_bib11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.plasmid.2003.09.003","article-title":"Mu dI1(Ap lac) mutagenesis of Yersinia pestis plasmid pFra and identification of temperature-regulated loci associated with virulence","volume":"51","author":"Welkos","year":"2004","journal-title":"Plasmid"},{"key":"10.1016\/j.pep.2004.11.001_bib12","doi-asserted-by":"crossref","first-page":"4994","DOI":"10.1128\/jb.173.16.4994-5009.1991","article-title":"Analysis of virC, an operon involved in the secretion of Yop proteins by Yersinia enterocolitica","volume":"173","author":"Michiels","year":"1991","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.pep.2004.11.001_bib13","doi-asserted-by":"crossref","first-page":"4820","DOI":"10.1128\/jb.174.14.4820-4828.1992","article-title":"Structure and regulation of the Yersinia pestis yscBCDEF operon","volume":"174","author":"Haddix","year":"1992","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.pep.2004.11.001_bib14","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1146\/annurev.micro.54.1.735","article-title":"Assembly and function of type III secretory systems","volume":"54","author":"Cornelis","year":"2000","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.pep.2004.11.001_bib15","first-page":"325","article-title":"Construction of defined F1 negative mutants of virulent Yersinia pestis","volume":"13","author":"Worsham","year":"1995","journal-title":"Contrib. Microbiol. Immunol."},{"key":"10.1016\/j.pep.2004.11.001_bib16","doi-asserted-by":"crossref","first-page":"109","DOI":"10.4269\/ajtmh.1994.51.109","article-title":"Cat-transmitted fatal pneumonic plague in a person who traveled from Colorado to Arizona","volume":"51","author":"Doll","year":"1994","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.pep.2004.11.001_bib17","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1016\/S0264-410X(98)80110-2","article-title":"Protection against experimental bubonic and pneumonic plague by a recombinant capsular F1-V antigen fusion protein vaccine","volume":"16","author":"Heath","year":"1998","journal-title":"Vaccine"},{"key":"10.1016\/j.pep.2004.11.001_bib18","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.1128\/IAI.67.3.1533-1537.1999","article-title":"Protective efficacy of recombinant Yersinia outer proteins against bubonic plague caused by encapsulated and nonencapsulated Yersinia pestis","volume":"67","author":"Andrews","year":"1999","journal-title":"Infect. Immun."},{"key":"10.1016\/j.pep.2004.11.001_bib19","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1006\/prep.2001.1467","article-title":"Overexpression and purification of Helicobacter pylori flavodoxin and induction of a specific antiserum in rabbits","volume":"22","author":"Paul","year":"2001","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.pep.2004.11.001_bib20","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/S1046-5928(03)00103-7","article-title":"Purification and characterization of the beta-trefoil fold protein barley alpha-amylase\/subtilisin inhibitor overexpressed in Escherichia coli","volume":"30","author":"Bonsager","year":"2003","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.pep.2004.11.001_bib21","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1038\/31972","article-title":"NMR structure and mutagenesis of the FADD (Mort1) death-effector domain","volume":"392","author":"Eberstadt","year":"1998","journal-title":"Nature"},{"key":"10.1016\/j.pep.2004.11.001_bib22","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.pep.2003.12.005","article-title":"Expression cloned cDNA for 10-deacetylbaccatin III-10-O-acetyltransferase in Escherichia coli: a comparative study of three fusion systems","volume":"35","author":"Fang","year":"2004","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.pep.2004.11.001_bib23","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1089\/153685903771797048","article-title":"Effect of imidazole on the solubility of a his-tagged antibody fragment","volume":"22","author":"Hamilton","year":"2003","journal-title":"Hybrid. Hybridomics"},{"key":"10.1016\/j.pep.2004.11.001_bib24","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1023\/A:1020424023207","article-title":"Differential effects of supplementary affinity tags on the solubility of MBP fusion proteins","volume":"2","author":"Routzahn","year":"2002","journal-title":"J. Struct. Funct. Genomics"},{"key":"10.1016\/j.pep.2004.11.001_bib25","doi-asserted-by":"crossref","first-page":"2402","DOI":"10.1038\/sj.bjc.6601888","article-title":"Modifying an immunogenic epitope on a therapeutic protein: a step towards an improved system for antibody-directed enzyme prodrug therapy (ADEPT)","volume":"90","author":"Mayer","year":"2004","journal-title":"Br. J. Cancer"},{"key":"10.1016\/j.pep.2004.11.001_bib26","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/S0304-4165(00)00086-6","article-title":"Relative position of the hexahistidine tag effects binding properties of a tumor-associated single-chain Fv construct","volume":"1523","author":"Goel","year":"2000","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.pep.2004.11.001_bib27","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0168-1656(00)00311-4","article-title":"Immunogenic efficacy of differently produced recombinant vaccines candidates against Pseudomonas aeruginosa infections","volume":"83","author":"von Specht","year":"2000","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.pep.2004.11.001_bib28","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1002\/(SICI)1096-9888(199808)33:8<697::AID-JMS711>3.0.CO;2-H","article-title":"Determination of protein\u2013protein interactions by matrix-assisted laser desorption\/ionization mass spectrometry","volume":"33","author":"Farmer","year":"1998","journal-title":"J. Mass. Spectrom."},{"key":"10.1016\/j.pep.2004.11.001_bib29","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.4049\/jimmunol.168.3.1315","article-title":"Yersinia enterocolitica evasion of the host innate immune response by V antigen-induced IL-10 production of macrophages is abrogated in IL-10-deficient mice","volume":"168","author":"Sing","year":"2002","journal-title":"J. Immunol."},{"key":"10.1016\/j.pep.2004.11.001_bib30","unstructured":"B.S. Powell, J.T. Enama, S. Jendrek, J.W. Farchaus, P.L. Worsham, J.K. Pullen, S.T. Strachan, J.J. Adamovicz, G.P. Andrews, Purification and characterization of recombinant Yersinia pestis F1-V fusion protein, Protein Exp. Purif., in preparation"},{"key":"10.1016\/j.pep.2004.11.001_bib31","unstructured":"G.P. Andrews, B.S. Powell, C. Bolt, S.L. Welkos, J.J. Adamovicz, Potency, stability, and safety of recombinant Yersinia pestis F1-V fusion protein vaccine as assessed in the mouse model for plague, Vaccine, in preparation"}],"container-title":["Protein Expression and Purification"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S104659280400378X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S104659280400378X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,4,4]],"date-time":"2020-04-04T19:24:41Z","timestamp":1586028281000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S104659280400378X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,3]]},"references-count":31,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2005,3]]}},"alternative-id":["S104659280400378X"],"URL":"https:\/\/doi.org\/10.1016\/j.pep.2004.11.001","relation":{},"ISSN":["1046-5928"],"issn-type":[{"value":"1046-5928","type":"print"}],"subject":[],"published":{"date-parts":[[2005,3]]}}}