{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T19:51:40Z","timestamp":1758397900917},"reference-count":44,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2006,9,1]],"date-time":"2006-09-01T00:00:00Z","timestamp":1157068800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Expression and Purification"],"published-print":{"date-parts":[[2006,9]]},"DOI":"10.1016\/j.pep.2006.04.011","type":"journal-article","created":{"date-parts":[[2006,5,3]],"date-time":"2006-05-03T13:58:17Z","timestamp":1146664697000},"page":"121-128","source":"Crossref","is-referenced-by-count":9,"title":["Expression and purification of functional human anthrax toxin receptor (ATR\/TEM8) binding domain from Escherichia coli"],"prefix":"10.1016","volume":"49","author":[{"given":"Zhiping","family":"Ding","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth A.","family":"Bradley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Amin Arnaout","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian-Ping","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.pep.2006.04.011_bib1","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1038\/nm1201-1271","article-title":"Bioterrorism: a clear and present danger","volume":"7","author":"Lane","year":"2001","journal-title":"Nat. Med."},{"key":"10.1016\/j.pep.2006.04.011_bib2","first-page":"21","article-title":"Bacillus anthracis evolution and epidemiology","volume":"271","author":"Keim","year":"2002","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"10.1016\/j.pep.2006.04.011_bib3","first-page":"1","article-title":"Introduction: anthrax history, disease and ecology","volume":"271","author":"Turnbull","year":"2002","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"10.1016\/j.pep.2006.04.011_bib4","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1146\/annurev.micro.55.1.647","article-title":"Anthrax","volume":"55","author":"Mock","year":"2001","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.pep.2006.04.011_bib5","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/S1286-4579(99)80004-5","article-title":"Molecular pathogenesis of Bacillus anthracis infection","volume":"1","author":"Little","year":"1999","journal-title":"Microbes. Infect."},{"key":"10.1016\/j.pep.2006.04.011_bib6","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/S0966-842X(99)01507-3","article-title":"Understanding Bacillus anthracis pathogenesis","volume":"7","author":"Hanna","year":"1999","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.pep.2006.04.011_bib7","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/S0966-842X(00)01755-8","article-title":"Discovery of the anthrax toxin: the beginning of in vivo studies on pathogenic bacteria","volume":"8","author":"Smith","year":"2000","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.pep.2006.04.011_bib8","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1128\/IAI.67.2.964-967.1999","article-title":"Functional analysis of the carboxy-terminal domain of Bacillus anthracis protective antigen","volume":"67","author":"Brossier","year":"1999","journal-title":"Infect. Immun."},{"key":"10.1016\/j.pep.2006.04.011_bib9","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1093\/infdis\/115.5.481","article-title":"Role of the lymphatics in the pathogenesis of anthrax","volume":"115","author":"Lincoln","year":"1965","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.pep.2006.04.011_bib10","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1093\/infdis\/116.4.401","article-title":"Cytological features of anthrax spores phagocytized in vitro by rabbit alveolar macrophages","volume":"116","author":"Shafa","year":"1966","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.pep.2006.04.011_bib11","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1016\/S0002-9440(10)61218-7","article-title":"Bacillus anthracis edema toxin causes extensive tissue lesions and rapid lethality in mice","volume":"167","author":"Firoved","year":"2005","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.pep.2006.04.011_bib12","doi-asserted-by":"crossref","first-page":"3162","DOI":"10.1073\/pnas.79.10.3162","article-title":"Anthrax toxin edema factor: a bacterial adenylate cyclase that increases cyclic amp concentrations of eukaryotic cells","volume":"79","author":"Leppla","year":"1982","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.pep.2006.04.011_bib13","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1126\/science.280.5364.734","article-title":"Proteolytic inactivation of map-kinase-kinase by anthrax lethal factor","volume":"280","author":"Duesbery","year":"1998","journal-title":"Science"},{"key":"10.1016\/j.pep.2006.04.011_bib14","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1006\/bbrc.1998.9040","article-title":"Anthrax lethal factor cleaves the N-terminus of MAPKKs and induces tyrosine\/threonine phosphorylation of MAPKs in cultured macrophages","volume":"248","author":"Vitale","year":"1998","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.pep.2006.04.011_bib15","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1038\/n35101999","article-title":"Identification of the cellular receptor for anthrax toxin","volume":"414","author":"Bradley","year":"2001","journal-title":"Nature"},{"key":"10.1016\/j.pep.2006.04.011_bib16","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1126\/science.289.5482.1197","article-title":"Genes expressed in human tumor endothelium","volume":"289","author":"St Croix","year":"2000","journal-title":"Science"},{"key":"10.1016\/j.pep.2006.04.011_bib17","first-page":"6649","article-title":"Cell surface tumor endothelial markers are conserved in mice and humans","volume":"61","author":"Carson-Walter","year":"2001","journal-title":"Cancer Res."},{"key":"10.1016\/j.pep.2006.04.011_bib18","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.yexcr.2004.12.025","article-title":"Tem8 expression stimulates endothelial cell adhesion and migration by regulating cell\u2013matrix interactions on collagen","volume":"305","author":"Hotchkiss","year":"2005","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.pep.2006.04.011_bib19","doi-asserted-by":"crossref","first-page":"2755","DOI":"10.1242\/jcs.114.15.2755","article-title":"Differential gene expression during capillary morphogenesis in 3D collagen matrices: regulated expression of genes involved in basement membrane matrix assembly, cell cycle progression, cellular differentiation and g-protein signaling","volume":"114","author":"Bell","year":"2001","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.pep.2006.04.011_bib20","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1086\/378781","article-title":"Mutations in capillary morphogenesis gene-2 result in the allelic disorders juvenile hyaline fibromatosis and infantile systemic hyalinosis","volume":"73","author":"Dowling","year":"2003","journal-title":"Am. J. Hum. Genet."},{"key":"10.1016\/j.pep.2006.04.011_bib21","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1086\/378418","article-title":"Mutations in the gene encoding capillary morphogenesis protein 2 cause juvenile hyaline fibromatosis and infantile systemic hyalinosis","volume":"73","author":"Hanks","year":"2003","journal-title":"Am. J. Hum. Genet."},{"key":"10.1016\/j.pep.2006.04.011_bib22","doi-asserted-by":"crossref","first-page":"5170","DOI":"10.1073\/pnas.0431098100","article-title":"Human capillary morphogenesis protein 2 functions as an anthrax toxin receptor","volume":"100","author":"Scobie","year":"2003","journal-title":"PNAS"},{"key":"10.1016\/j.pep.2006.04.011_bib23","doi-asserted-by":"crossref","first-page":"5227","DOI":"10.1074\/jbc.M210321200","article-title":"Cell surface tumor endothelium marker 8 cytoplasmic tail-independent anthrax toxin binding, proteolytic processing, oligomer formation, and internalization","volume":"278","author":"Liu","year":"2003","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.pep.2006.04.011_bib24","doi-asserted-by":"crossref","first-page":"49342","DOI":"10.1074\/jbc.M307900200","article-title":"Binding of anthrax toxin to its receptor is similar to alpha integrin\u2013ligand interactions","volume":"278","author":"Bradley","year":"2003","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.pep.2006.04.011_bib25","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/0092-8674(95)90517-0","article-title":"Crystal structure of the a-domain from the alpha subunit of integrin cr3 (cd11b\/cd18)","volume":"80","author":"Lee","year":"1995","journal-title":"Cell"},{"key":"10.1016\/j.pep.2006.04.011_bib26","doi-asserted-by":"crossref","first-page":"1642","DOI":"10.1046\/j.1538-7836.2003.00277.x","article-title":"Integrins, cations and ligands: making the connection","volume":"1","author":"Xiong","year":"2003","journal-title":"J. Thromb. Haemost."},{"key":"10.1016\/j.pep.2006.04.011_bib27","doi-asserted-by":"crossref","first-page":"6367","DOI":"10.1073\/pnas.0401506101","article-title":"Crystal structure of the von Willebrand factor a domain of human capillary morphogenesis protein. 2. An anthrax toxin receptor","volume":"101","author":"Lacy","year":"2004","journal-title":"PNAS"},{"key":"10.1016\/j.pep.2006.04.011_bib28","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1038\/nature02763","article-title":"Crystal structure of a complex between anthrax toxin and its host cell receptor","volume":"430","author":"Santelli","year":"2004","journal-title":"Nature"},{"key":"10.1016\/j.pep.2006.04.011_bib29","doi-asserted-by":"crossref","first-page":"13147","DOI":"10.1073\/pnas.0405405101","article-title":"Structure of heptameric protective antigen bound to an anthrax toxin receptor: a role for receptor in pH-dependent pore formation","volume":"101","author":"Lacy","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.pep.2006.04.011_bib30","doi-asserted-by":"crossref","first-page":"1333","DOI":"10.1016\/S0969-2126(01)00271-4","article-title":"Two conformations of the integrin a-domain (i-domain): a pathway for activation?","volume":"3","author":"Lee","year":"1995","journal-title":"Structure"},{"key":"10.1016\/j.pep.2006.04.011_bib31","doi-asserted-by":"crossref","first-page":"38762","DOI":"10.1074\/jbc.C000563200","article-title":"An isoleucine-based allosteric switch controls affinity and shape shifting in integrin cd11b a-domain","volume":"275","author":"Xiong","year":"2000","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.pep.2006.04.011_bib32","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/S0092-8674(00)80622-4","article-title":"Structural basis of collagen recognition by integrin alpha2beta1","volume":"101","author":"Emsley","year":"2000","journal-title":"Cell"},{"key":"10.1016\/j.pep.2006.04.011_bib33","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/S0092-8674(02)01257-6","article-title":"Structures of the alpha l i domain and its complex with icam-1 reveal a shape-shifting pathway for integrin regulation","volume":"112","author":"Shimaoka","year":"2003","journal-title":"Cell"},{"key":"10.1016\/j.pep.2006.04.011_bib34","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1086\/432731","article-title":"A soluble receptor decoy protects rats against anthrax lethal toxin challenge","volume":"192","author":"Scobie","year":"2005","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.pep.2006.04.011_bib35","first-page":"9","article-title":"The Bradford method for protein quantitation","volume":"32","author":"Kruger","year":"1994","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.pep.2006.04.011_bib36","doi-asserted-by":"crossref","first-page":"23349","DOI":"10.1074\/jbc.M401292200","article-title":"Binding stoichiometry and kinetics of the interaction of a human anthrax toxin receptor, cmg2, with protective antigen","volume":"279","author":"Wigelsworth","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.pep.2006.04.011_bib37","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/0378-1119(88)90005-4","article-title":"Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase","volume":"67","author":"Smith","year":"1988","journal-title":"Gene"},{"key":"10.1016\/j.pep.2006.04.011_bib38","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1016\/S1016-8478(23)13487-X","article-title":"Maltose binding protein (MBP) fusion proteins with low or no affinity to amylose resins can be single-step purified using a novel anti-MBP monoclonal antibody","volume":"8","author":"Park","year":"1998","journal-title":"Mol. Cells"},{"key":"10.1016\/j.pep.2006.04.011_bib39","first-page":"99","article-title":"Maltose-binding protein as a solubility enhancer","volume":"205","author":"Fox","year":"2003","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.pep.2006.04.011_bib40","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.jbiotec.2004.08.004","article-title":"Advanced genetic strategies for recombinant protein expression in Escherichia coli","volume":"115","author":"Sorensen","year":"2005","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.pep.2006.04.011_bib41","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1016\/0958-1669(91)90046-8","article-title":"Optimizing protein folding to the native state in bacteria","volume":"2","author":"Schein","year":"1991","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.pep.2006.04.011_bib42","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1006\/prep.1996.0678","article-title":"Expression of aggregation-prone recombinant proteins at low temperatures: a comparative study of the Escherichia coli cspa and tac promoter systems","volume":"9","author":"Vasina","year":"1997","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.pep.2006.04.011_bib43","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1586\/14737159.1.1.11","article-title":"Maldi TOF mass spectrometry: an emerging platform for genomics and diagnostics","volume":"1","author":"Leushner","year":"2001","journal-title":"Expert Rev. Mol. Diagn."},{"key":"10.1016\/j.pep.2006.04.011_bib44","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1042\/bst0270335","article-title":"BIAcore: an affinity biosensor system for characterization of biomolecular interactions","volume":"27","author":"Malmqvist","year":"1999","journal-title":"Biochem. Soc. Trans."}],"container-title":["Protein Expression and Purification"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592806001227?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592806001227?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,2,4]],"date-time":"2024-02-04T05:40:22Z","timestamp":1707025222000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046592806001227"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,9]]},"references-count":44,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2006,9]]}},"alternative-id":["S1046592806001227"],"URL":"https:\/\/doi.org\/10.1016\/j.pep.2006.04.011","relation":{},"ISSN":["1046-5928"],"issn-type":[{"value":"1046-5928","type":"print"}],"subject":[],"published":{"date-parts":[[2006,9]]}}}