{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T09:08:13Z","timestamp":1785834493114,"version":"3.56.0"},"reference-count":31,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:00:00Z","timestamp":1172707200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Expression and Purification"],"published-print":{"date-parts":[[2007,3]]},"DOI":"10.1016\/j.pep.2006.09.016","type":"journal-article","created":{"date-parts":[[2006,10,24]],"date-time":"2006-10-24T17:58:15Z","timestamp":1161712695000},"page":"89-95","source":"Crossref","is-referenced-by-count":22,"title":["Molecular cloning, expression and purification of l-amino acid oxidase from the Malayan pit viper Calloselasma rhodostoma"],"prefix":"10.1016","volume":"52","author":[{"given":"Phaneeswara Rao","family":"Kommoju","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Macheroux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandro","family":"Ghisla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.pep.2006.09.016_bib1","doi-asserted-by":"crossref","first-page":"1888","DOI":"10.1002\/hlca.194402701241","article-title":"\u00dcber eine neue L-Aminos\u00e4ure-oxydase","volume":"27","author":"Zeller","year":"1944","journal-title":"Helv. Chim. Acta"},{"key":"10.1016\/j.pep.2006.09.016_bib2","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1038\/2251072a0","article-title":"l-amino-acid oxidase, a bactericidal system","volume":"225","author":"Skarnes","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.pep.2006.09.016_bib3","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/S0014-5793(02)03608-6","article-title":"Antimicrobial action of achacin is mediated by l-amino acid oxidase activity","volume":"531","author":"Ehara","year":"2002","journal-title":"FEBS Lett."},{"key":"10.1016\/j.pep.2006.09.016_bib4","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S0014-5793(99)00327-0","article-title":"Macromolecular antimicrobial glycoprotein, achacin, expressed in a methylotrophic yeast Pichia pastoris","volume":"448","author":"Ogawa","year":"1999","journal-title":"FEBS Lett."},{"key":"10.1016\/j.pep.2006.09.016_bib5","doi-asserted-by":"crossref","first-page":"3609","DOI":"10.1242\/jeb.01795","article-title":"Cloning, characterization and expression of escapin, a broadly antimicrobial FAD-containing l-amino acid oxidase from ink of the sea hare Aplysia californica","volume":"208","author":"Yang","year":"2005","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.pep.2006.09.016_bib6","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1016\/S0041-0101(02)00102-2","article-title":"Snake venom l-amino acid oxidases","volume":"40","author":"Du","year":"2002","journal-title":"Toxicon"},{"key":"10.1016\/j.pep.2006.09.016_bib7","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1006\/bbrc.1993.1023","article-title":"Induction of apoptosis by hemorrhagic snake venom in vascular endothelial cells","volume":"190","author":"Araki","year":"1993","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.pep.2006.09.016_bib8","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S0300-9084(02)01374-3","article-title":"Apoptosis-inducing factor (AIF): a novel caspase-independent death effector released from mitochondria","volume":"84","author":"Cande","year":"2002","journal-title":"Biochimie"},{"key":"10.1016\/j.pep.2006.09.016_bib9","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/S0145-305X(02)00140-4","article-title":"l-Amino acid oxidase activity of an antineoplastic factor of a marine mollusk and its relationship to cytotoxicity","volume":"27","author":"Iijima","year":"2003","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.pep.2006.09.016_bib10","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/S0887-2333(03)00010-9","article-title":"Apoptotic effect in the glioma cells induced by specific protein extracted from Okinawa Habu (Trimeresurus flavoviridis) venom in relation to oxidative stress","volume":"17","author":"Sun","year":"2003","journal-title":"Toxicol. In Vitro"},{"key":"10.1016\/j.pep.2006.09.016_bib11","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1107\/S0907444904000046","article-title":"Purification, partial characterization, crystallization and structural determination of AHP-LAAO, a novel l-amino-acid oxidase with cell apoptosis-inducing activity from Agkistrodon halys pallas venom","volume":"60","author":"Zhang","year":"2004","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.pep.2006.09.016_bib12","doi-asserted-by":"crossref","first-page":"4561","DOI":"10.4049\/jimmunol.173.7.4561","article-title":"IL-4-induced gene-1 is a leukocyte l-amino acid oxidase with an unusual acidic pH preference and lysosomal localization","volume":"173","author":"Mason","year":"2004","journal-title":"J. Immunol."},{"key":"10.1016\/j.pep.2006.09.016_bib13","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.abb.2003.12.007","article-title":"Achacin induces cell death in HeLa cells through two different mechanisms","volume":"422","author":"Kanzawa","year":"2004","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.pep.2006.09.016_bib14","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1055\/s-0038-1657280","article-title":"Impairment of platelet aggregation by Echis colorata venom mediated by l-amino acid oxidase or H2O2","volume":"48","author":"Nathan","year":"1982","journal-title":"Thrombosis & Haemostasis"},{"key":"10.1016\/j.pep.2006.09.016_bib15","doi-asserted-by":"crossref","first-page":"1827","DOI":"10.1016\/S0041-0101(01)00133-7","article-title":"Inhibition of human platelet aggregation by l-amino acid oxidase purified from Naja naja kaouthia venom","volume":"39","author":"Sakurai","year":"2001","journal-title":"Toxicon"},{"key":"10.1016\/j.pep.2006.09.016_bib16","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1016\/j.bbrc.2003.08.044","article-title":"Molecular characterization of Trimeresurus stejnegeri venom l-amino acid oxidase with potential anti-HIV activity","volume":"309","author":"Zhang","year":"2003","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.pep.2006.09.016_bib17","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1021\/bi00832a015","article-title":"Studies on l-amino acid oxidase. II. Dissociation and characterization of its subunits","volume":"8","author":"deKok","year":"1969","journal-title":"Biochemistry"},{"key":"10.1016\/j.pep.2006.09.016_bib18","doi-asserted-by":"crossref","first-page":"4204","DOI":"10.1093\/emboj\/19.16.4204","article-title":"The structure of l-amino acid oxidase reveals the substrate trajectory into an enantiomerically conserved active site","volume":"19","author":"Pawelek","year":"2000","journal-title":"EMBO J"},{"key":"10.1016\/j.pep.2006.09.016_bib19","doi-asserted-by":"crossref","first-page":"4044","DOI":"10.1046\/j.1432-1327.2001.02321.x","article-title":"Structure and characterization of the glycan moiety of l-amino acid oxidase from the Malayan pit viper Calloselasma rhodostoma","volume":"268","author":"Geyer","year":"2001","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.pep.2006.09.016_bib20","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/S1046-5928(02)00701-5","article-title":"Heterologous expression of Rhodococcus opacus l-amino acid oxidase in Streptomyces lividans","volume":"28","author":"Geueke","year":"2003","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.pep.2006.09.016_bib21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1432-1033.1992.tb17254.x","article-title":"Molecular cloning of the antibacterial protein of the giant African snail, Achatina fulica Ferussac","volume":"209","author":"Obara","year":"1992","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.pep.2006.09.016_bib22","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.1128\/AEM.67.4.1470-1475.2001","article-title":"Purification, characterization, and application of a novel dye-linked l-proline dehydrogenase from a hyperthermophilic archaeon, Thermococcus profundus","volume":"67","author":"Sakuraba","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.pep.2006.09.016_bib23","doi-asserted-by":"crossref","first-page":"8035","DOI":"10.1016\/S0021-9258(17)40931-8","article-title":"Reversible inactivation of l-amino acid oxidase. Properties of the three conformational forms","volume":"252","author":"Coles","year":"1977","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.pep.2006.09.016_bib24","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.1046\/j.1432-1327.2001.02042.x","article-title":"l-amino-acid oxidase from the Malayan pit viper Calloselasma rhodostoma. Comparative sequence analysis and characterization of active and inactive forms of the enzyme","volume":"268","author":"Macheroux","year":"2001","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.pep.2006.09.016_bib25","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1006\/abio.2001.5381","article-title":"A spectrophotometric microplate assay for l-amino acid oxidase","volume":"298","author":"Kishimoto","year":"2001","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.pep.2006.09.016_bib26","series-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook","year":"1989"},{"key":"10.1016\/j.pep.2006.09.016_bib27","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1007\/s10495-006-7959-9","article-title":"Mechanisms of cell death induction by l-amino acid oxidase, a major component of ophidian venom","volume":"11","author":"Ande","year":"2006","journal-title":"Apoptosis"},{"key":"10.1016\/j.pep.2006.09.016_bib28","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1111\/j.1574-6976.2000.tb00532.x","article-title":"Heterologous protein expression in the methylotrophic yeast Pichia pastoris","volume":"24","author":"Cereghino","year":"2000","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.pep.2006.09.016_bib29","doi-asserted-by":"crossref","first-page":"2013","DOI":"10.1016\/S0021-9258(18)69355-X","article-title":"Crystalline l-amino acid oxidase of Crotalus adamanteus","volume":"235","author":"Wellner","year":"1960","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.pep.2006.09.016_bib30","doi-asserted-by":"crossref","first-page":"3197","DOI":"10.1021\/bi992416z","article-title":"Molecular cloning and functional analysis of apoxin I, a snake venom- derived apoptosis-inducing factor with l-amino acid oxidase activity","volume":"39","author":"Torii","year":"2000","journal-title":"Biochemistry"},{"key":"10.1016\/j.pep.2006.09.016_bib31","doi-asserted-by":"crossref","first-page":"2084","DOI":"10.1128\/JB.187.6.2084-2092.2005","article-title":"Molecular analysis of the rebeccamycin l-amino acid oxidase from Lechevalieria aerocolonigenes ATCC 39243","volume":"187","author":"Nishizawa","year":"2005","journal-title":"J. Bacteriol."}],"container-title":["Protein Expression and Purification"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592806003068?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592806003068?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,8,3]],"date-time":"2021-08-03T21:31:07Z","timestamp":1628026267000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046592806003068"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,3]]},"references-count":31,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,3]]}},"alternative-id":["S1046592806003068"],"URL":"https:\/\/doi.org\/10.1016\/j.pep.2006.09.016","relation":{},"ISSN":["1046-5928"],"issn-type":[{"value":"1046-5928","type":"print"}],"subject":[],"published":{"date-parts":[[2007,3]]}}}