{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T15:23:21Z","timestamp":1759937001544},"reference-count":55,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2007,10,1]],"date-time":"2007-10-01T00:00:00Z","timestamp":1191196800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Expression and Purification"],"published-print":{"date-parts":[[2007,10]]},"DOI":"10.1016\/j.pep.2007.07.001","type":"journal-article","created":{"date-parts":[[2007,7,14]],"date-time":"2007-07-14T12:40:02Z","timestamp":1184416802000},"page":"262-272","source":"Crossref","is-referenced-by-count":3,"title":["Expression, purification and characterization of recombinant phospholipase B from Moraxella bovis with anomalous electrophoretic behavior"],"prefix":"10.1016","volume":"55","author":[{"given":"Brian J.","family":"Shiell","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary","family":"Tachedjian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerri","family":"Bruce","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary","family":"Beddome","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacinta L.","family":"Farn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter A.","family":"Hoyne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wojtek P.","family":"Michalski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.pep.2007.07.001_bib1","first-page":"761","article-title":"Infectious bovine keratoconjuctivitis: I. Etiology","volume":"21","author":"Henson","year":"1960","journal-title":"Am. J. Vet. Res."},{"key":"10.1016\/j.pep.2007.07.001_bib2","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/S0007-1935(17)32882-8","article-title":"Infectious bovine keratoconjuctivitis: a review","volume":"135","author":"Baptista","year":"1981","journal-title":"Br. Vet. J."},{"key":"10.1016\/j.pep.2007.07.001_bib3","series-title":"Pathogenesis of Bacterial Infections of Animals","first-page":"343","article-title":"Moraxella","author":"Michalski","year":"2004"},{"key":"10.1016\/j.pep.2007.07.001_bib4","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1111\/j.1939-1676.1998.tb02120.x","article-title":"Infectious bovine keratoconjunctivitis: a review","volume":"12","author":"Brown","year":"1998","journal-title":"J. Vet. Intern. Med."},{"key":"10.1016\/j.pep.2007.07.001_bib5","first-page":"883","article-title":"Production and characterization of Moraxella bovis hemolysin","volume":"38","author":"Sandhu","year":"1977","journal-title":"Am. J. Vet. Res."},{"key":"10.1016\/j.pep.2007.07.001_bib6","doi-asserted-by":"crossref","first-page":"2601","DOI":"10.1128\/jb.172.5.2601-2607.1990","article-title":"Morphogenetic expression of Moraxella bovis fimbriae (pili) in Pseudomonas aeruginosa","volume":"172","author":"Beard","year":"1990","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.pep.2007.07.001_bib7","doi-asserted-by":"crossref","first-page":"6717","DOI":"10.1128\/JB.183.22.6717-6720.2001","article-title":"Molecular characterisation of a secreted enzyme with phospholipase activity from Moraxella bovis","volume":"183","author":"Farn","year":"2001","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.pep.2007.07.001_bib8","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/j.vaccine.2004.06.025","article-title":"Prevention of naturally occurring infectious bovine keratoconjunctivitis with a recombinant Moraxella bovis cytotoxin-ISCOM matrix adjuvanted vaccine","volume":"23","author":"Angelos","year":"2004","journal-title":"Vaccine"},{"key":"10.1016\/j.pep.2007.07.001_bib9","doi-asserted-by":"crossref","first-page":"136","DOI":"10.2460\/ajvr.2005.66.136","article-title":"Effectiveness of a cytolysin-enriched vaccine for protection of cattle against infectious bovine keratoconjunctivitis","volume":"66","author":"George","year":"2005","journal-title":"Am. J. Vet. Res."},{"key":"10.1016\/j.pep.2007.07.001_bib10","doi-asserted-by":"crossref","first-page":"1678","DOI":"10.1128\/IAI.58.6.1678-1684.1990","article-title":"Characterization of the pilin gene of Moraxella bovis Dalton 2d and expression of pili from M. bovis in Pseudomonas aeruginosa","volume":"58","author":"Elleman","year":"1990","journal-title":"Infect. Immun."},{"key":"10.1016\/j.pep.2007.07.001_bib11","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/0378-1135(93)90138-W","article-title":"A Moraxella bovis pili vaccine produced by recombinant DNA technology for the prevention of infectious bovine keratoconjunctivitis","volume":"36","author":"Lepper","year":"1993","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.pep.2007.07.001_bib12","first-page":"A","article-title":"Protective efficacy of a Moraxella bovis haemolytic fraction in a challenge trial","volume":"14","author":"Billson","year":"1993","journal-title":"Aust. Microbiol."},{"key":"10.1016\/j.pep.2007.07.001_bib13","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1111\/j.1574-6968.1994.tb07263.x","article-title":"A haemolytic cell-free preparation of Moraxella bovis confers protection against infectious bovine keratoconjunctivitis","volume":"124","author":"Billson","year":"1994","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.pep.2007.07.001_bib14","doi-asserted-by":"crossref","first-page":"3469","DOI":"10.1128\/IAI.68.6.3469-3474.2000","article-title":"Characterization of the hemolysin of Moraxella bovis using a hemolysis-neutralizing monoclonal antibody","volume":"68","author":"Billson","year":"2000","journal-title":"Infection Immun."},{"key":"10.1016\/j.pep.2007.07.001_bib15","unstructured":"J.L. Farn, A genetic analysis of the virulence determinants of Moraxella bovis, PhD thesis, The University of Melbourne, Department of Microbiology and Immunology, 2000."},{"key":"10.1016\/j.pep.2007.07.001_bib16","doi-asserted-by":"crossref","first-page":"4341","DOI":"10.1128\/IAI.71.8.4341-4350.2003","article-title":"Identification of a Moraxella catarrahalis outer membrane protein exhibiting both adhesin and lipolytic activities","volume":"71","author":"Timpe","year":"2003","journal-title":"Infection Immun."},{"key":"10.1016\/j.pep.2007.07.001_bib17","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1128\/IAI.01330-06","article-title":"The Moraxella catarrhalis autotransporter McaP is a conserved surface protein that mediates adherence to the human epithelial cells through its N-terminal passenger domain","volume":"75","author":"Lipski","year":"2007","journal-title":"Infect. Immun."},{"key":"10.1016\/j.pep.2007.07.001_bib18","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1128\/MMBR.68.4.692-744.2004","article-title":"Type V protein secretion pathway: the autotransporter story, Microbiol","volume":"68","author":"Henderson","year":"2004","journal-title":"Mol. Biol. Rev."},{"key":"10.1016\/j.pep.2007.07.001_bib19","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1038\/sj.emboj.7600148","article-title":"Structure of the translocator domain of a bacterial autotransporter","volume":"23","author":"Oomen","year":"2004","journal-title":"EMBO J."},{"key":"10.1016\/j.pep.2007.07.001_bib20","doi-asserted-by":"crossref","first-page":"2814","DOI":"10.1110\/ps.051628705","article-title":"Misfolding of a bacterial autotransporter","volume":"14","author":"Mogensen","year":"2005","journal-title":"Protein Sci."},{"key":"10.1016\/j.pep.2007.07.001_bib21","doi-asserted-by":"crossref","first-page":"18872","DOI":"10.1073\/pnas.0509583102","article-title":"A selection for mutants that interfere with folding of Escherichia coli thioredoxin-1 in vivo","volume":"102","author":"Huber","year":"2005","journal-title":"Proc. Natl. Acad. Sci USA"},{"key":"10.1016\/j.pep.2007.07.001_bib22","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of the bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.pep.2007.07.001_bib23","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1006\/abio.1999.4292","article-title":"Microscale purification of proteins exhibiting anomalous electrophoretic migration: application to the analysis of GAP-43 phosphorylation","volume":"274","author":"Tejero-Diez","year":"1999","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.pep.2007.07.001_bib24","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.bbrc.2003.12.086","article-title":"Biophysical characterisation of Gir2, a highly acidic protein of Saccharomyces cerevisiae with anomalous electrophoretic behavior","volume":"314","author":"Alves","year":"2004","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.pep.2007.07.001_bib25","doi-asserted-by":"crossref","first-page":"7557","DOI":"10.1016\/S0021-9258(19)45811-0","article-title":"The molecular weights of vertebrate histones exploiting a modified sodium dodecyl sulfate electrophoretic method","volume":"246","author":"Panyim","year":"1971","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.pep.2007.07.001_bib26","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1111\/j.1432-1033.1987.tb11439.x","article-title":"Chemical modification of charged amino acid moieties alters the electrophoretic mobilities of neurofilament subunits on SDS\/polyacrylamide gels","volume":"165","author":"Georges","year":"1987","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.pep.2007.07.001_bib27","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/0003-9861(92)90639-E","article-title":"Modification of acidic residues normalizes sodium dodecyl sulfate-polyacrylamide gel electrophoresis of caldesmon and other proteins that migrate anomalously","volume":"297","author":"Graceffa","year":"1992","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.pep.2007.07.001_bib28","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/0166-0934(95)00085-1","article-title":"The conserved, hydrophilic and arginine-rich N-terminal domain of cucumovirus coat proteins contributes to their anomalous electrophoretic mobilities in sodium dodecyl\u2013polyacrylamide gels","volume":"55","author":"Hu","year":"1995","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.pep.2007.07.001_bib29","series-title":"Membrane Transport\u2014A practical approach","first-page":"141","article-title":"The amplified expression, identification, purification, assay and properties of histidine-tagged bacterial membrane transport proteins","author":"Ward","year":"2000"},{"key":"10.1016\/j.pep.2007.07.001_bib30","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1110\/ps.40101","article-title":"Aberrant mobility phenomena of the DNA repair protein XPA","volume":"10","author":"Iakoucheva","year":"2001","journal-title":"Protein Sci."},{"key":"10.1016\/j.pep.2007.07.001_bib31","series-title":"Gel Electrophoresis of Proteins. A Practical Approach","author":"Hames","year":"1990"},{"key":"10.1016\/j.pep.2007.07.001_bib32","doi-asserted-by":"crossref","first-page":"2052","DOI":"10.1046\/j.1432-1033.2002.02853.x","article-title":"ORF6 from the clavulinic acid gene cluster of Streptomyces clavuligerus has ornithine acetyltransferase activity","volume":"269","author":"Kershaw","year":"2002","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.pep.2007.07.001_bib33","doi-asserted-by":"crossref","first-page":"10510","DOI":"10.1021\/bi025579n","article-title":"High-risk (HPV16) human papillovirus E7 oncoprotein is highly stable and extended, with conformational transitions that could explain its multiple cellular binding partners","volume":"41","author":"Alonso","year":"2002","journal-title":"Biochemistry"},{"key":"10.1016\/j.pep.2007.07.001_bib34","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1042\/0264-6021:3630243","article-title":"Molecular dissection of membrane-transport proteins: mass spectrometry and sequence determination of the galactose-H+ symport protein, GalP, of Escherichia coli and quantitative assay of the incorporation of [ring-2-13 C] histidine and15 NH3","volume":"363","author":"Venter","year":"2002","journal-title":"Biochem. J."},{"key":"10.1016\/j.pep.2007.07.001_bib35","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/0378-1097(92)90433-O","article-title":"Anomalous electrophoretic behavior of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi in relation to its apparent molecular mass","volume":"95","author":"Martinez","year":"1992","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.pep.2007.07.001_bib36","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1128\/JB.138.3.663-670.1979","article-title":"Glycogen, hyaluronate, and some other polysaccharides greatly enhance the formation of exolipase by Serratia marcescens","volume":"138","author":"Winkler","year":"1979","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.pep.2007.07.001_bib37","doi-asserted-by":"crossref","first-page":"1070","DOI":"10.1128\/jb.168.3.1070-1074.1986","article-title":"Purification of extracellular lipase from Pseudomonas aeruginosa","volume":"168","author":"Stuer","year":"1986","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.pep.2007.07.001_bib38","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0003-2670(98)00486-3","article-title":"Strategies for analysis of electrophoretically separated proteins and peptides","volume":"383","author":"Michalski","year":"1999","journal-title":"Anal. Chimica Acta"},{"key":"10.1016\/j.pep.2007.07.001_bib39","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0166-0934(01)00441-4","article-title":"Mass spectrometric identification and characterisation of the nucleocapsid protein of Menangle virus","volume":"102","author":"Shiell","year":"2002","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.pep.2007.07.001_bib40","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1002\/elps.1150090106","article-title":"Improved silver staining procedure for fast staining in Phast System Development Unit. I. Staining of sodium dodecylsulfate gels","volume":"9","author":"Heukeshoven","year":"1988","journal-title":"Electrophoresis"},{"key":"10.1016\/j.pep.2007.07.001_bib41","doi-asserted-by":"crossref","first-page":"7014","DOI":"10.1128\/JB.181.22.7014-7020.1999","article-title":"Characterization of the essential transport function of the AIDA-I autotransporter and evidence supporting structural predictions","volume":"181","author":"Maurer","year":"1999","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.pep.2007.07.001_bib42","doi-asserted-by":"crossref","first-page":"14497","DOI":"10.1073\/pnas.0404679101","article-title":"Evidence for conservation of architecture and physical properties of Omp85-like proteins throughout evolution","volume":"101","author":"Surana","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.pep.2007.07.001_bib43","doi-asserted-by":"crossref","first-page":"11248","DOI":"10.1021\/bi0491898","article-title":"Structural basis of protein kinetic stability: resistance to sodium dodecyl sulfate suggests a central role for rigidity and a bias toward \u03b2-sheet structure","volume":"43","author":"Manning","year":"2004","journal-title":"Biochemistry"},{"key":"10.1016\/j.pep.2007.07.001_bib44","doi-asserted-by":"crossref","first-page":"2745","DOI":"10.1021\/ac048494w","article-title":"Protein pI shifts due to posttranslational modifications in the separation and characterization of proteins","volume":"77","author":"Zhu","year":"2005","journal-title":"Anal. Chem."},{"key":"10.1016\/j.pep.2007.07.001_bib45","unstructured":"L. Kozlowski, Calculation of protein isoelectric point, http:\/\/isoelectric.ovh.org\/."},{"key":"10.1016\/j.pep.2007.07.001_bib46","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1006\/jmbi.1998.1893","article-title":"Lambda repressor N-terminal DNA-binding domain as an assay for protein transmembrane segment interaction in Vivo","volume":"280","author":"Leeds","year":"1998","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.pep.2007.07.001_bib47","doi-asserted-by":"crossref","first-page":"3701","DOI":"10.1093\/nar\/gkg519","article-title":"GlobPlot: exploring protein sequences for globularity and disorder","volume":"31","author":"Linding","year":"2003","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.pep.2007.07.001_bib48","doi-asserted-by":"crossref","first-page":"W177","DOI":"10.1093\/nar\/gkl266","article-title":"DISULFIND: a disulfide bonding state and cysteine connectivity prediction server","volume":"34","author":"Ceroni","year":"2006","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.pep.2007.07.001_bib49","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1099\/mic.0.28548-0","article-title":"Paired cysteine residues are required for high levels of the Helicobacter pylori autotransporter VacA","volume":"152","author":"Letley","year":"2006","journal-title":"Microbiology"},{"key":"10.1016\/j.pep.2007.07.001_bib50","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1111\/j.1365-2958.1995.mmi_18020378.x","article-title":"Common structural features of IgA1 protease-like outer membrane protein autotransporters","volume":"18","author":"Jose","year":"1995","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.pep.2007.07.001_bib51","doi-asserted-by":"crossref","first-page":"1631","DOI":"10.1002\/elps.1150191019","article-title":"Electrophoresis of proteins across a transverse sodium dodecyl sulfate gradient","volume":"19","author":"Gianazza","year":"1998","journal-title":"Electrophoresis"},{"key":"10.1016\/j.pep.2007.07.001_bib52","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1002\/(SICI)1522-2683(20000501)21:8<1435::AID-ELPS1435>3.0.CO;2-E","article-title":"Interactions between carbonic anhydrase and its inhibitors revealed by gel electrophoresis and circular dichroism","volume":"21","author":"Gianazza","year":"2000","journal-title":"Electrophoresis"},{"key":"10.1016\/j.pep.2007.07.001_bib53","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/BF01025552","article-title":"Factors determining the reconstructive denaturation of proteins in sodium dodecyl sulfate solutions. Further circular dichroism studies on structural reorganization of seven proteins","volume":"1","author":"Jirgensons","year":"1982","journal-title":"J. Prot. Chem."},{"key":"10.1016\/j.pep.2007.07.001_bib54","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/BF01025375","article-title":"Circular dichroism and conformational properties of modeccin, a toxic protein","volume":"2","author":"Jirgensons","year":"1983","journal-title":"J. Prot. Chem."},{"key":"10.1016\/j.pep.2007.07.001_bib55","doi-asserted-by":"crossref","first-page":"11008","DOI":"10.1073\/pnas.96.20.11008","article-title":"Kinetic stability as a mechanism for protease longevity","volume":"96","author":"Cunningham","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"}],"container-title":["Protein Expression and Purification"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592807001660?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592807001660?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,8,19]],"date-time":"2021-08-19T06:29:21Z","timestamp":1629354561000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046592807001660"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,10]]},"references-count":55,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2007,10]]}},"alternative-id":["S1046592807001660"],"URL":"https:\/\/doi.org\/10.1016\/j.pep.2007.07.001","relation":{},"ISSN":["1046-5928"],"issn-type":[{"value":"1046-5928","type":"print"}],"subject":[],"published":{"date-parts":[[2007,10]]}}}