{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T06:30:35Z","timestamp":1788849035159,"version":"build-2803163510"},"reference-count":34,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2008,6,1]],"date-time":"2008-06-01T00:00:00Z","timestamp":1212278400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Expression and Purification"],"published-print":{"date-parts":[[2008,6]]},"DOI":"10.1016\/j.pep.2008.02.014","type":"journal-article","created":{"date-parts":[[2008,3,7]],"date-time":"2008-03-07T00:07:13Z","timestamp":1204848433000},"page":"309-313","source":"Crossref","is-referenced-by-count":6,"title":["Expression, purification and immunodetection of a recombinant fragment (residues 179\u2013281) of the G protein from rabies virus ERA strain"],"prefix":"10.1016","volume":"59","author":[{"given":"\u00canio J.","family":"Bassi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Vernal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila","family":"Zanluca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hern\u00e1n","family":"Terenzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos R.","family":"Zanetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.pep.2008.02.014_bib1","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1016\/S0140-6736(04)15792-9","article-title":"Rabies and other lyssavirus diseases","volume":"363","author":"Warrel","year":"2004","journal-title":"The Lancet"},{"key":"10.1016\/j.pep.2008.02.014_bib2","series-title":"Laboratory Techniques in Rabies","first-page":"28","article-title":"Characteristics and molecular biology of the rabies virus","author":"Tordo","year":"1996"},{"key":"10.1016\/j.pep.2008.02.014_bib3","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1038\/294275a0","article-title":"Structure of the glycoprotein gene in rabies virus","volume":"294","author":"Anilionis","year":"1981","journal-title":"Nature"},{"key":"10.1016\/j.pep.2008.02.014_bib4","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1007\/BF01314961","article-title":"The glycoprotein G of rhabdoviruses","volume":"140","author":"Coll","year":"1995","journal-title":"Arch. Virol."},{"key":"10.1016\/j.pep.2008.02.014_bib5","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1016\/0042-6822(92)90465-2","article-title":"Rabies glycoprotein is a trimer","volume":"187","author":"Gaudin","year":"1992","journal-title":"Virology"},{"key":"10.1016\/j.pep.2008.02.014_bib6","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.virusres.2005.05.002","article-title":"DNA vaccine for rabies: relevance of the trans-membrane domain of the glycoprotein in generating an antibody response","volume":"113","author":"Rath","year":"2005","journal-title":"Virus Res."},{"key":"10.1016\/j.pep.2008.02.014_bib7","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/S0264-410X(85)90224-5","article-title":"Rabies immunosomes (subunit vaccine) structure and immunogenicity. Pre- and post-exposure protection studies","volume":"3","author":"Perrin","year":"1985","journal-title":"Vaccine"},{"key":"10.1016\/j.pep.2008.02.014_bib8","doi-asserted-by":"crossref","first-page":"269","DOI":"10.4049\/jimmunol.110.1.269","article-title":"Antigenic properties of rabies virus components","volume":"110","author":"Wiktor","year":"1973","journal-title":"J. Immunol."},{"key":"10.1016\/j.pep.2008.02.014_bib9","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1128\/IAI.16.3.754-759.1977","article-title":"Rabies virus glycoprotein. II Biological and serological characterization","volume":"16","author":"Cox","year":"1977","journal-title":"Infect. Immun."},{"key":"10.1016\/j.pep.2008.02.014_bib10","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/0161-5890(86)90084-2","article-title":"Stimulation of cytotoxic T-lymphocyte responses by rabies virus glycoprotein and identification of an immunodominant domain","volume":"23","author":"Macfarlan","year":"1986","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.pep.2008.02.014_bib11","doi-asserted-by":"crossref","first-page":"3128","DOI":"10.1128\/JVI.62.9.3128-3134.1988","article-title":"Recognition of rabies and rabies-related virus by T cells derived from human vaccine recipients","volume":"62","author":"Celis","year":"1988","journal-title":"J. Virol."},{"key":"10.1016\/j.pep.2008.02.014_bib12","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1099\/0022-1317-70-2-291","article-title":"Characterization of a new virus-neutralizing epitope that denotes a sequential determinant on the rabies virus glycoprotein","volume":"70","author":"Bunschoten","year":"1989","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.pep.2008.02.014_bib13","doi-asserted-by":"crossref","first-page":"3804","DOI":"10.1128\/JVI.64.8.3804-3809.1990","article-title":"Structural and immunological characterization of a linear virus-neutralizing epitope of the rabies virus glycoprotein and its possible use in a synthetic vaccine","volume":"64","author":"Dietzschold","year":"1990","journal-title":"J. Virol."},{"key":"10.1016\/j.pep.2008.02.014_bib14","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1099\/0022-1317-74-8-1539","article-title":"Structural and functional studies on a unique linear neutralizing antigenic site (G5) of the rabies virus glycoprotein","volume":"74","author":"Van der Heijden","year":"1993","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.pep.2008.02.014_bib15","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0168-1702(97)00080-4","article-title":"A virus-neutralizing epitope on the glycoprotein of rabies virus that contains Trp251 is a linear epitope","volume":"51","author":"Luo","year":"1997","journal-title":"Virus Res."},{"key":"10.1016\/j.pep.2008.02.014_bib16","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1099\/0022-1317-77-2-339","article-title":"Immunodominant epitopes defined by a yeast-expressed library of random fragments of the rabies virus glycoprotein map outside major antigenic sites","volume":"77","author":"Lafay","year":"1996","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.pep.2008.02.014_bib17","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1111\/j.1348-0421.1995.tb03258.x","article-title":"Mapping and characterization of a sequential epitope on the rabies virus glycoprotein which is recognized by a neutralizing monoclonal antibody, RG719","volume":"39","author":"Ni","year":"1995","journal-title":"Microbiol. Immunol."},{"key":"10.1016\/j.pep.2008.02.014_bib18","doi-asserted-by":"crossref","first-page":"4672","DOI":"10.1128\/JVI.79.8.4672-4678.2005","article-title":"Novel rabies virus-neutralizing epitope recognized by human monoclonal antibody: fine mapping and escape mutant analysis","volume":"79","author":"Marissen","year":"2005","journal-title":"J. Virol."},{"key":"10.1016\/j.pep.2008.02.014_bib19","doi-asserted-by":"crossref","first-page":"2663","DOI":"10.1099\/0022-1317-83-11-2663","article-title":"Canine vaccine recipients recognize an immunodominant region of the rabies virus glycoprotein","volume":"83","author":"Johnson","year":"2002","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.pep.2008.02.014_bib20","doi-asserted-by":"crossref","first-page":"3026","DOI":"10.1016\/j.vaccine.2004.11.071","article-title":"Production of rabies neutralizing antibody in hen\u2019s eggs using a part of the G protein expressed in Escherichia coli","volume":"23","author":"Motoi","year":"2005","journal-title":"Vaccine"},{"key":"10.1016\/j.pep.2008.02.014_bib21","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1006\/viro.1995.1289","article-title":"Immune responses to nucleic acid vaccines to rabies virus","volume":"209","author":"Xiang","year":"1995","journal-title":"Virology"},{"key":"10.1016\/j.pep.2008.02.014_bib22","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1006\/prep.1997.0796","article-title":"Divergent effects of chaperone overexpression and ethanol supplementation on inclusion body formation in recombinant Escherichia coli","volume":"11","author":"Thomas","year":"1997","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.pep.2008.02.014_bib23","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/0003-2697(87)90587-2","article-title":"Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100kDa","volume":"166","author":"Sh\u00e4gger","year":"1987","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.pep.2008.02.014_bib24","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.pep.2008.02.014_bib25","series-title":"Proteomics in Practice. A Laboratory Manual of Proteome Analysis","author":"Westermeier","year":"2002"},{"key":"10.1016\/j.pep.2008.02.014_bib26","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.pep.2008.02.014_bib27","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1590\/S0036-46651993000200009","article-title":"Simplified fluorescence inhibition microtest for the titration of rabies neutralizing antibodies","volume":"35","author":"Favoretto","year":"1993","journal-title":"Rev. Inst. Med. Trop. Sao Paulo"},{"key":"10.1016\/j.pep.2008.02.014_bib28","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1126\/science.6297004","article-title":"Rabies virus glycoprotein analogs: biosynthesis in Escherichia coli","volume":"219","author":"Yelverton","year":"1983","journal-title":"Science"},{"key":"10.1016\/j.pep.2008.02.014_bib29","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/S0264-410X(98)00196-0","article-title":"Studies on the structures and antigenic properties of rabies virus glycoprotein analogues produced in yeast cells","volume":"17","author":"Sakamoto","year":"1999","journal-title":"Vaccine"},{"key":"10.1016\/j.pep.2008.02.014_bib30","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1002\/biot.200600211","article-title":"Rabies virus glycoprotein expression in Drosophila S2 cells. I. Functional recombinant protein in stable co-transfected cell line","volume":"2","author":"Yokomizo","year":"2007","journal-title":"Biotechnol. J."},{"key":"10.1016\/j.pep.2008.02.014_bib31","first-page":"279","article-title":"An attenuated rabies vaccine for domestic animals produced in tissue culture","volume":"5","author":"Abelseth","year":"1964","journal-title":"Can. Vet. J."},{"key":"10.1016\/j.pep.2008.02.014_bib32","series-title":"Laboratory Techniques in Rabies","first-page":"271","article-title":"Cell-culture vaccines for human use: general considerations","author":"Nicholson","year":"1996"},{"key":"10.1016\/j.pep.2008.02.014_bib33","series-title":"Laboratory Techniques in Rabies","first-page":"360","article-title":"The NIH test for potency","author":"Wilbur","year":"1996"},{"key":"10.1016\/j.pep.2008.02.014_bib34","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/0264-410X(88)90185-5","article-title":"NIH test, a problematic method for testing potency of inactivated rabies vaccine","volume":"6","author":"Barth","year":"1988","journal-title":"Vaccine"}],"container-title":["Protein Expression and Purification"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592808000582?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592808000582?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,2,23]],"date-time":"2024-02-23T07:54:14Z","timestamp":1708674854000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046592808000582"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,6]]},"references-count":34,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2008,6]]}},"alternative-id":["S1046592808000582"],"URL":"https:\/\/doi.org\/10.1016\/j.pep.2008.02.014","relation":{},"ISSN":["1046-5928"],"issn-type":[{"value":"1046-5928","type":"print"}],"subject":[],"published":{"date-parts":[[2008,6]]}}}