{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T11:04:23Z","timestamp":1778929463400,"version":"3.51.4"},"reference-count":15,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Expression and Purification"],"published-print":{"date-parts":[[2010,4]]},"DOI":"10.1016\/j.pep.2009.09.005","type":"journal-article","created":{"date-parts":[[2009,9,14]],"date-time":"2009-09-14T08:07:16Z","timestamp":1252915636000},"page":"179-183","source":"Crossref","is-referenced-by-count":7,"title":["Engineering, expression, and immuno-characterization of recombinant protein comprising multi-neutralization sites of rabies virus glycoprotein"],"prefix":"10.1016","volume":"70","author":[{"given":"Xiangdong","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinyan","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuiming","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yafeng","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaodu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xufang","family":"Deng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuehui","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weidong","family":"Bao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peihong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinping","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chan","family":"Ding","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiyong","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.pep.2009.09.005_bib1","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/S1473-3099(02)00287-6","article-title":"Rabies re-examined","volume":"2","author":"Rupprecht","year":"1998","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.pep.2009.09.005_bib2","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1093\/clinids\/10.Supplement_4.S771","article-title":"The molecular biology of rabies viruses","volume":"10","author":"Wunner","year":"1988","journal-title":"Rev. Infect. Dis."},{"key":"10.1016\/j.pep.2009.09.005_bib3","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1073\/pnas.80.1.70","article-title":"Characterization of an antigenic determinant of the glycoprotein that correlates with pathogenicity of rabies virus","volume":"80","author":"Dietzschold","year":"1983","journal-title":"Proc. Nat. Acad. Sci. USA"},{"key":"10.1016\/j.pep.2009.09.005_bib4","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1128\/IAI.16.3.754-759.1977","article-title":"Rabies virus glycoprotein II. Biological and serological characterization","volume":"16","author":"Cox","year":"1977","journal-title":"Infect. Immun."},{"key":"10.1016\/j.pep.2009.09.005_bib5","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/S0264-410X(85)90224-5","article-title":"Rabies immunosomes (subunit vaccine) structure and immunogenicity. Pre- and post-exposure protection studies","volume":"3","author":"Perrin","year":"1985","journal-title":"Vaccine"},{"key":"10.1016\/j.pep.2009.09.005_bib6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/JVI.62.1.1-7.1988","article-title":"Antigenic site II of the rabies virus glycoprotein: structure and role in viral virulence","volume":"62","author":"Prehaud","year":"1988","journal-title":"J. Virol."},{"key":"10.1016\/j.pep.2009.09.005_bib7","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1128\/JVI.53.3.926-934.1985","article-title":"Rabies virulence effect on pathogenicity and sequence characterization of rabies virus mutations affecting antigenic site III of the glycoprotein","volume":"53","author":"Seif","year":"1985","journal-title":"J. Virol."},{"key":"10.1016\/j.pep.2009.09.005_bib8","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1128\/JCM.27.5.899-902.1989","article-title":"Enzyme-linked immunosorbent assay for determination of antibodies to the envelope glycoprotein of rabies virus","volume":"27","author":"Grassi","year":"1989","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.pep.2009.09.005_bib9","doi-asserted-by":"crossref","first-page":"3026","DOI":"10.1016\/j.vaccine.2004.11.071","article-title":"Production of rabies neutralizing antibody in hen\u2019s eggs using a part of the G protein expressed in Escherichia coli","volume":"23","author":"Motoi","year":"2005","journal-title":"Vaccine"},{"key":"10.1016\/j.pep.2009.09.005_bib10","doi-asserted-by":"crossref","first-page":"2663","DOI":"10.1099\/0022-1317-83-11-2663","article-title":"Canine vaccine recipients recognize an immunodominant region of the rabies virus glycoprotein","volume":"83","author":"Johnson","year":"2002","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.pep.2009.09.005_bib11","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.ab.2007.10.021","article-title":"Splicing together different regions of a gene by modified polymerase chain reaction-based site-directed mutagenesis","volume":"373","author":"Li","year":"2008","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.pep.2009.09.005_bib12","series-title":"Laboratory Techniques in Rabies","author":"Meslin","year":"1996"},{"key":"10.1016\/j.pep.2009.09.005_bib13","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.jviromet.2006.08.006","article-title":"Expression of the nucleoprotein gene of rabies virus for use as a diagnostic reagent","volume":"138","author":"He","year":"2006","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.pep.2009.09.005_bib14","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1007\/s11010-007-9603-6","article-title":"Production of active eukaryotic proteins through bacterial expression systems: a review of the existing biotechnology strategies","volume":"307","author":"Sahdev","year":"2008","journal-title":"Mol. Cell. Biochem."},{"key":"10.1016\/j.pep.2009.09.005_bib15","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1023\/A:1025024104862","article-title":"Protein aggregation in recombinant bacteria: biological role of inclusion bodies","volume":"17","author":"Villaverde","year":"2003","journal-title":"Biotechnol. Lett."}],"container-title":["Protein Expression and Purification"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592809002204?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592809002204?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,5,22]],"date-time":"2020-05-22T04:45:37Z","timestamp":1590122737000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046592809002204"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4]]},"references-count":15,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2010,4]]}},"alternative-id":["S1046592809002204"],"URL":"https:\/\/doi.org\/10.1016\/j.pep.2009.09.005","relation":{},"ISSN":["1046-5928"],"issn-type":[{"value":"1046-5928","type":"print"}],"subject":[],"published":{"date-parts":[[2010,4]]}}}