{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T16:35:49Z","timestamp":1772814949148,"version":"3.50.1"},"reference-count":20,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Expression and Purification"],"published-print":{"date-parts":[[2010,8]]},"DOI":"10.1016\/j.pep.2010.03.018","type":"journal-article","created":{"date-parts":[[2010,3,30]],"date-time":"2010-03-30T17:47:13Z","timestamp":1269971233000},"page":"169-174","source":"Crossref","is-referenced-by-count":28,"title":["Heterologous expression and characterization of recombinant OsCDR1, a rice aspartic proteinase involved in disease resistance"],"prefix":"10.1016","volume":"72","author":[{"given":"Bishun Deo","family":"Prasad","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary","family":"Creissen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris","family":"Lamb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bharat B.","family":"Chattoo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.pep.2010.03.018_bib1","first-page":"189","article-title":"The structure and function of the aspartic proteinases","volume":"19","author":"Davies","year":"1990","journal-title":"Annu. Rev."},{"key":"10.1016\/j.pep.2010.03.018_bib2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1749-6632.1992.tb27472.x","article-title":"Cellular proteolysis: an overview","volume":"674","author":"Barrett","year":"1992","journal-title":"Ann. NY Acad. Sci."},{"key":"10.1016\/j.pep.2010.03.018_bib3","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1093\/nar\/27.1.325","article-title":"MEROPS: the peptidase database","volume":"27","author":"Rawlings","year":"1999","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.pep.2010.03.018_bib4","series-title":"FEBS Advanced Course 84\/07","first-page":"1","article-title":"Aspartic proteinases and their inhibitors","author":"John","year":"1985"},{"key":"10.1016\/j.pep.2010.03.018_bib5","series-title":"Aspartic Proteinases and Their Inhibitors, FEBS Advanced Course 84\/07","first-page":"19","article-title":"Comments on the nomenclature of aspartic proteinases","author":"Foltman","year":"1985"},{"key":"10.1016\/j.pep.2010.03.018_bib6","doi-asserted-by":"crossref","first-page":"493","DOI":"10.2174\/138920305774933268","article-title":"Aspartic proteinase content of the Arabidopsis genome","volume":"6","author":"Faro","year":"2005","journal-title":"Curr. Protein Pept. Sci."},{"key":"10.1016\/j.pep.2010.03.018_bib7","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1034\/j.1399-3054.1999.105324.x","article-title":"Plant aspartic proteinases: enzymes on the way to a function","volume":"105","author":"Mutlu","year":"1999","journal-title":"Physiol. Plant"},{"key":"10.1016\/j.pep.2010.03.018_bib8","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1023\/A:1005833207707","article-title":"Molecular organization of a gene in barley which encodes a protein similar to aspartic protease and its specific expression in nucellar cells during degeneration","volume":"35","author":"Chen","year":"1997","journal-title":"Plant Mol. Biol."},{"key":"10.1016\/j.pep.2010.03.018_bib9","doi-asserted-by":"crossref","first-page":"2067","DOI":"10.1111\/j.1432-1033.2004.04136.x","article-title":"Structure and function of plant aspartic proteinases","volume":"271","author":"Sim\u00f5es","year":"2004","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.pep.2010.03.018_bib10","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1094\/MPMI-22-12-1635","article-title":"Overexpression of rice (Oryza sativa L.) OsCDR1 leads to constitutive activation of defence responses in rice and Arabidopsis","volume":"22","author":"Prasad","year":"2009","journal-title":"Mol. Plant Microbe Interact."},{"key":"10.1016\/j.pep.2010.03.018_bib11","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1146\/annurev.phyto.42.040803.140421","article-title":"Systemic acquired resistance","volume":"42","author":"Durrant","year":"2004","journal-title":"Annu. Rev. Phytopathol."},{"key":"10.1016\/j.pep.2010.03.018_bib12","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1038\/nature00962","article-title":"A putative lipid transfer protein involved in systemic resistance signalling in Arabidopsis","volume":"419","author":"Maldonado","year":"2002","journal-title":"Nature"},{"key":"10.1016\/j.pep.2010.03.018_bib13","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2-\u0394\u0394Ct method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.pep.2010.03.018_bib14","series-title":"Methods for Research on the Ecology of Soil-Borne Plant Pathogens","author":"Johnson","year":"1972"},{"key":"10.1016\/j.pep.2010.03.018_bib15","doi-asserted-by":"crossref","first-page":"31358","DOI":"10.1074\/jbc.M702477200","article-title":"Characterization of recombinant CDR1, an Arabidopsis aspartic proteinase involved in disease resistance","volume":"282","author":"Sim\u00f5es","year":"2007","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.pep.2010.03.018_bib16","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1023\/A:1015049629736","article-title":"An aspartic protease with antimicrobial activity is induced after infection and wounding in intercellular fluids of potato tubers","volume":"108","author":"Guevara","year":"2002","journal-title":"Eur. J. Plant Pathol."},{"key":"10.1016\/j.pep.2010.03.018_bib17","first-page":"233","article-title":"Potato aspartic proteases: induction, antimicrobial activity and substrate specificity","volume":"86","author":"Guevara","year":"2004","journal-title":"J. Plant Pathol."},{"key":"10.1016\/j.pep.2010.03.018_bib18","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1099\/mic.0.28816-0","article-title":"Antimicrobial activity of potato aspartic proteases (StAPs) involves membrane permeabilization","volume":"152","author":"Mendieta","year":"2006","journal-title":"Microbiology"},{"key":"10.1016\/j.pep.2010.03.018_bib19","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1038\/sj.emboj.7600086","article-title":"An extracellular aspartic protease functions in Arabidopsis disease resistance signalling","volume":"23","author":"Xia","year":"2004","journal-title":"EMBO J."},{"key":"10.1016\/j.pep.2010.03.018_bib20","series-title":"Digestive Physiology of Fish","author":"Ozaki","year":"1983"}],"container-title":["Protein Expression and Purification"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592810000938?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592810000938?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,12,12]],"date-time":"2018-12-12T06:22:39Z","timestamp":1544595759000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046592810000938"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,8]]},"references-count":20,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2010,8]]}},"alternative-id":["S1046592810000938"],"URL":"https:\/\/doi.org\/10.1016\/j.pep.2010.03.018","relation":{},"ISSN":["1046-5928"],"issn-type":[{"value":"1046-5928","type":"print"}],"subject":[],"published":{"date-parts":[[2010,8]]}}}