{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T13:05:12Z","timestamp":1784034312353,"version":"3.55.0"},"reference-count":37,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2013BAD12B01"],"award-info":[{"award-number":["2013BAD12B01"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010581","name":"General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010581","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012225","name":"Chinese Academy of Inspection and Quarantine","doi-asserted-by":"publisher","award":["2013JK005"],"award-info":[{"award-number":["2013JK005"]}],"id":[{"id":"10.13039\/501100012225","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012225","name":"Chinese Academy of Inspection and Quarantine","doi-asserted-by":"publisher","award":["2012JK011"],"award-info":[{"award-number":["2012JK011"]}],"id":[{"id":"10.13039\/501100012225","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Protein Expression and Purification"],"published-print":{"date-parts":[[2013,11]]},"DOI":"10.1016\/j.pep.2013.08.012","type":"journal-article","created":{"date-parts":[[2013,8,26]],"date-time":"2013-08-26T11:47:02Z","timestamp":1377517622000},"page":"1-8","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":27,"title":["Expression and purification of the nucleocapsid protein of Schmallenberg virus, and preparation and characterization of a monoclonal antibody against this protein"],"prefix":"10.1016","volume":"92","author":[{"given":"Yongning","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoqiang","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianchang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerstin","family":"Wernike","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jizhou","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyan","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jihong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caixia","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junhua","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangfen","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangmei","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"2012","key":"10.1016\/j.pep.2013.08.012_b0005","first-page":"469","article-title":"Novel orthobunyavirus in Cattle, Europe","volume":"18","author":"Hoffmann","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.pep.2013.08.012_b0010","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S0950268812002245","article-title":"\u2018Schmallenberg virus\u2019\u2013a novel orthobunyavirus emerging in Europe","volume":"141","author":"Beer","year":"2013","journal-title":"Epidemiol. Infect."},{"key":"10.1016\/j.pep.2013.08.012_b0015","first-page":"112","article-title":"Diarrhea and loss of production on Dutch dairy farms caused by the Schmallenberg virus","volume":"137","author":"Muskens","year":"2012","journal-title":"Tijdschr. Diergeneeskd."},{"key":"10.1016\/j.pep.2013.08.012_b0020","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1177\/0300985812447831","article-title":"Salient lesions in domestic ruminants infected with the emerging so-called Schmallenberg virus in Germany","volume":"49","author":"Herder","year":"2012","journal-title":"Vet. Pathol."},{"key":"10.1016\/j.pep.2013.08.012_b0025","doi-asserted-by":"crossref","first-page":"e1003133","DOI":"10.1371\/journal.ppat.1003133","article-title":"Schmallenberg virus pathogenesis, tropism and interaction with the innate immune system of the host","volume":"9","author":"Varela","year":"2013","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.pep.2013.08.012_b0030","first-page":"175","article-title":"Schmallenberg Virus","volume":"135","author":"Wernike","year":"2013","journal-title":"Dev. Biol. (Basel)"},{"key":"10.1016\/j.pep.2013.08.012_b0035","unstructured":"OIE. OIE Technical Factsheet Schmallenberg Virus, February 2013. http:\/\/www.oie.int\/fileadmin\/Home\/eng\/Our_scientific_expertise\/docs\/pdf\/A_Schmallenberg_virus.pdf. OIE, Paris, France (2013)."},{"key":"10.1016\/j.pep.2013.08.012_b0040","doi-asserted-by":"crossref","DOI":"10.1007\/s11250-013-0415-2","article-title":"Antibodies to Schmallenberg virus in domestic livestock in Turkey","author":"Azkur","year":"2013","journal-title":"Trop. Anim. Health Prod."},{"key":"10.1016\/j.pep.2013.08.012_b0045","doi-asserted-by":"crossref","first-page":"2802","DOI":"10.1128\/JCM.00877-13","article-title":"Presence of antibodies to Schmallenberg virus in a dog in sweden","volume":"51","author":"Wensman","year":"2013","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.pep.2013.08.012_b0050","doi-asserted-by":"crossref","first-page":"e40380","DOI":"10.1371\/journal.pone.0040380","article-title":"Schmallenberg virus infection of adult type I interferon receptor knock-out mice","volume":"7","author":"Wernike","year":"2012","journal-title":"PLoS ONE"},{"key":"10.1016\/j.pep.2013.08.012_b0055","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1111\/tbed.12000","article-title":"Detection of Schmallenberg virus in different Culicoides spp. by real-time RT-PCR","volume":"59","author":"De Regge","year":"2012","journal-title":"Transbound. Emerg. Dis."},{"key":"10.1016\/j.pep.2013.08.012_b0060","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.3201\/eid1807.120385","article-title":"Culicoids as vectors of Schmallenberg virus","volume":"18","author":"Rasmussen","year":"2012","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.pep.2013.08.012_b0065","doi-asserted-by":"crossref","first-page":"106","DOI":"10.3201\/eid1901.121054","article-title":"Schmallenberg virus in Culicoides spp. biting midges, the Netherlands, 2011","volume":"19","author":"Elbers","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.pep.2013.08.012_b0070","doi-asserted-by":"crossref","first-page":"e57747","DOI":"10.1371\/journal.pone.0057747","article-title":"Implicating Culicoides biting midges as vectors of Schmallenberg virus using semi-quantitative RT-PCR","volume":"8","author":"Veronesi","year":"2013","journal-title":"PLoS ONE"},{"issue":"2012","key":"10.1016\/j.pep.2013.08.012_b0075","first-page":"1512","article-title":"Schmallenberg virus in domestic cattle, Belgium","volume":"18","author":"Garigliany","year":"2012","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.pep.2013.08.012_b0080","first-page":"1005","article-title":"Schmallenberg virus in calf born at term with porencephaly","volume":"18","author":"Garigliany","year":"2012","journal-title":"Belgium Emerg. Infect. Dis."},{"key":"10.1016\/j.pep.2013.08.012_b0085","first-page":"106","article-title":"Epizootic of ovine congenital malformations associated with Schmallenberg virus infection","volume":"137","author":"van den Brom","year":"2012","journal-title":"Tijdschr. Diergeneeskd."},{"key":"10.1016\/j.pep.2013.08.012_b0090","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1080\/00480169.2012.738403","article-title":"Schmallenberg virus, a novel orthobunyavirus infection in ruminants in Europe: potential global impact and preventive measures","volume":"61","author":"Conraths","year":"2013","journal-title":"N. Z. Vet. J."},{"key":"10.1016\/j.pep.2013.08.012_b0095","doi-asserted-by":"crossref","first-page":"1644","DOI":"10.3201\/eid1810.120835","article-title":"Schmallenberg virus as possible ancestor of Shamonda virus","volume":"18","author":"Goller","year":"2012","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.pep.2013.08.012_b0100","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.1007\/s00705-012-1341-8","article-title":"Genetic reassortment between Sathuperi and Shamonda viruses of the genus Orthobunyavirus in nature: implications for their genetic relationship to Schmallenberg virus","volume":"157","author":"Yanase","year":"2012","journal-title":"Arch. Virol."},{"key":"10.1016\/j.pep.2013.08.012_b0105","doi-asserted-by":"crossref","first-page":"5912","DOI":"10.1093\/nar\/gkt268","article-title":"Nucleocapsid protein structures from orthobunyaviruses reveal insight into ribonucleoprotein architecture and RNA polymerization","volume":"41","author":"Ariza","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.pep.2013.08.012_b0110","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1186\/1297-9716-44-31","article-title":"Epidemiology, molecular virology and diagnostics of Schmallenberg virus, an emerging orthobunyavirus in Europe","volume":"44","author":"Doceul","year":"2013","journal-title":"Vet. Res."},{"key":"10.1016\/j.pep.2013.08.012_b0115","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1099\/vir.0.049908-0","article-title":"A mutation \u2018hot spot\u2019 in the Schmallenberg virus M segment","volume":"94","author":"Fischer","year":"2013","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.pep.2013.08.012_b0120","doi-asserted-by":"crossref","first-page":"5593","DOI":"10.1128\/JVI.00223-13","article-title":"Structure of Schmallenberg orthobunyavirus nucleoprotein suggests a novel mechanism of genome encapsidation","volume":"87","author":"Dong","year":"2013","journal-title":"J. Virol."},{"key":"10.1016\/j.pep.2013.08.012_b0125","doi-asserted-by":"crossref","first-page":"e53446","DOI":"10.1371\/journal.pone.0053446","article-title":"Validation of a commercially available indirect ELISA using a nucleocapside recombinant protein for detection of Schmallenberg virus antibodies","volume":"8","author":"Br\u00e9ard","year":"2013","journal-title":"PLoS ONE"},{"key":"10.1016\/j.pep.2013.08.012_b0130","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.vetmic.2012.03.035","article-title":"Organ distribution of Schmallenberg virus RNA in malformed newborns","volume":"159","author":"Bilk","year":"2012","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.pep.2013.08.012_b0135","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.pep.2011.10.012","article-title":"Production, purification and characterization of mouse monoclonal antibodies against human mitochondrial transcription termination factor 2 (MTERF2)","volume":"82","author":"Xiong","year":"2012","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.pep.2013.08.012_b0140","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.pep.2013.06.011","article-title":"Expression, purification and characterization of two truncated peste des petits ruminants virus matrix proteins in Escherichia coli, and production of polyclonal antibodies against this protein","volume":"91","author":"Liu","year":"2013","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.pep.2013.08.012_b0145","doi-asserted-by":"crossref","first-page":"613","DOI":"10.4161\/auto.7.6.15267","article-title":"Autophagy promotes the replication of encephalomyocarditis virus in host cells","volume":"7","author":"Zhang","year":"2011","journal-title":"Autophagy"},{"key":"10.1016\/j.pep.2013.08.012_b0150","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.virol.2012.12.011","article-title":"Peste des petits ruminants virus exploits cellular autophagy machinery for replication","volume":"437","author":"Zhang","year":"2013","journal-title":"Virology"},{"key":"10.1016\/j.pep.2013.08.012_b0155","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.antiviral.2012.05.014","article-title":"Schmallenberg virus: a new Shamonda\/Sathuperi-like virus on the rise in Europe","volume":"95","author":"Garigliany","year":"2012","journal-title":"Antiviral Res."},{"key":"10.1016\/j.pep.2013.08.012_b0160","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.antiviral.2012.06.001","article-title":"Molecular biology and genetic diversity of Rift Valley fever virus","volume":"95","author":"Ikegami","year":"2012","journal-title":"Antiviral Res."},{"key":"10.1016\/j.pep.2013.08.012_b0165","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1136\/vr.100883","article-title":"Preliminary estimate of Schmallenberg virus infection impact in sheep flocks \u2013 France","volume":"171","author":"Dominguez","year":"2012","journal-title":"Vet. Rec."},{"key":"10.1016\/j.pep.2013.08.012_b0170","article-title":"Field veterinary survey on clinical and economic impact of Schmallenberg virus in Belgium","author":"Martinelle","year":"2012","journal-title":"Transbound. Emerg. Dis."},{"key":"10.1016\/j.pep.2013.08.012_b0175","article-title":"Preliminary survey on the impact of Schmallenberg virus on sheep flocks in south of Belgium","author":"Saegerman","year":"2013","journal-title":"Transbound. Emerg. Dis."},{"key":"10.1016\/j.pep.2013.08.012_b0180","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority, \u201cSchmallenberg\u201d virus: Analysis of the epidemiological data and assessment of impact. EFSA J. 10 (2012) 2768 [89 pp.].","DOI":"10.2903\/j.efsa.2012.2768"},{"key":"10.1016\/j.pep.2013.08.012_b0185","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1023\/B:jsfg.0000031965.37625.0e","article-title":"His tag effect on solubility of human proteins produced in Escherichia coli: a comparison between four expression vectors","volume":"5","author":"Woestenenk","year":"2004","journal-title":"J. Struct. Funct. Genomics"}],"container-title":["Protein Expression and Purification"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592813001629?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592813001629?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T01:50:51Z","timestamp":1760925051000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046592813001629"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,11]]}},"alternative-id":["S1046592813001629"],"URL":"https:\/\/doi.org\/10.1016\/j.pep.2013.08.012","relation":{},"ISSN":["1046-5928"],"issn-type":[{"value":"1046-5928","type":"print"}],"subject":[],"published":{"date-parts":[[2013,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Expression and purification of the nucleocapsid protein of Schmallenberg virus, and preparation and characterization of a monoclonal antibody against this protein","name":"articletitle","label":"Article Title"},{"value":"Protein Expression and Purification","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.pep.2013.08.012","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}