{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T14:58:47Z","timestamp":1761663527601},"reference-count":18,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Fundaci\u00f3n Carlos Slim Helu"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Protein Expression and Purification"],"published-print":{"date-parts":[[2017,2]]},"DOI":"10.1016\/j.pep.2016.10.008","type":"journal-article","created":{"date-parts":[[2016,10,20]],"date-time":"2016-10-20T13:47:46Z","timestamp":1476971266000},"page":"129-136","update-policy":"http:\/\/dx.doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"special_numbering":"C","title":["Expression, purification, immunogenicity and protective efficacy of a recombinant nucleoside hydrolase from Leishmania donovani, a vaccine candidate for preventing cutaneous leishmaniasis"],"prefix":"10.1016","volume":"130","author":[{"given":"C. Patrick","family":"McAtee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher A.","family":"Seid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Molly","family":"Hammond","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elissa","family":"Hudspeth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian P.","family":"Keegan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuyun","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junfei","family":"Wei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Zhan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raul","family":"Arjona-Sabido","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Cruz-Chan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Dumonteil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Hotez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Elena","family":"Bottazzi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"10053","key":"10.1016\/j.pep.2016.10.008_bib1","first-page":"1448","article-title":"Global, regional, and national incidence, prevalence, and years lived with disability for 301 acute and chronic diseases and injuries in 188 countries, 1990-2013: a systematic analysis for the Global Burden of Disease Study 2013","volume":"388","author":"Global Burden of Disease Study","year":"2015","journal-title":"Lancet"},{"key":"10.1016\/j.pep.2016.10.008_bib2","doi-asserted-by":"crossref","first-page":"e2865","DOI":"10.1371\/journal.pntd.0002865","article-title":"The global burden of disease study 2010: interpretation and implications for the neglected tropical diseases","volume":"8","author":"Hotez","year":"2014","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.pep.2016.10.008_bib3","doi-asserted-by":"crossref","first-page":"2992","DOI":"10.1016\/j.vaccine.2015.12.071","article-title":"Status of vaccine research and development of vaccines for leishmaniasis","volume":"34","author":"Gillespie","year":"2016","journal-title":"Vaccine"},{"key":"10.1016\/j.pep.2016.10.008_bib4","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1007\/978-0-387-77570-8_10","article-title":"Antiparasitic chemotherapy: tinkering with the purine salvage pathway","volume":"625","author":"Datta","year":"2008","journal-title":"Adv. Exp. Med. Biol."},{"key":"10.1016\/j.pep.2016.10.008_bib5","doi-asserted-by":"crossref","first-page":"21114","DOI":"10.1074\/jbc.274.30.21114","article-title":"Nucleoside hydrolase from Leishmania major. Cloning, expression, catalytic properties, transition state inhibitors, and the 2.5-A crystal structure","volume":"274","author":"Shi","year":"1999","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.pep.2016.10.008_bib6","doi-asserted-by":"crossref","first-page":"e866","DOI":"10.1371\/journal.pntd.0000866","article-title":"Adaptive immunity against Leishmania nucleoside hydrolase maps its C-terminal domain as the target of the CD4+ T cell-driven protective response","volume":"4","author":"Nico","year":"2010","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.pep.2016.10.008_bib7","first-page":"1","article-title":"Cross-protective immunity to Leishmania amazonensis is mediated by CD4+ and CD8+ epitopes of Leishmania donovani nucleoside hydrolase terminal domains","volume":"5","author":"Nico","year":"2014","journal-title":"Front. Immunol."},{"key":"10.1016\/j.pep.2016.10.008_bib8","first-page":"1","article-title":"Leishmania donovani nucleoside hydrolase terminal domains in cross-protective immunotherapy against Leishmania amazonensis murine infection","volume":"5","author":"Nico","year":"2014","journal-title":"Front. Immunol."},{"key":"10.1016\/j.pep.2016.10.008_bib9","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1128\/IAI.73.2.812-819.2005","article-title":"Cross-protective efficacy of a prophylactic Leishmania donovani DNA vaccine against visceral and cutaneous murine leishmaniasis","volume":"73","author":"Aguilar-Be","year":"2005","journal-title":"Infect. Immun."},{"key":"10.1016\/j.pep.2016.10.008_bib10","doi-asserted-by":"crossref","first-page":"20658","DOI":"10.1016\/S0021-9258(18)54759-1","article-title":"Nucleoside hydrolase from Crithidia fasciculata. Metabolic role, purification, specificity, and kinetic mechanism","volume":"266","author":"Parkin","year":"1991","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.pep.2016.10.008_bib11","doi-asserted-by":"crossref","first-page":"1683","DOI":"10.1002\/pmic.200800562","article-title":"The detection, correlation, and comparison of peptide precursor and product ions from data independent LC-MS with data dependant LC-MS\/MS","volume":"9","author":"Geromanos","year":"2009","journal-title":"Proteomics"},{"key":"10.1016\/j.pep.2016.10.008_bib12","doi-asserted-by":"crossref","first-page":"2187","DOI":"10.1021\/ac048455k","article-title":"Quantitative proteomic analysis by accurate mass retention time pairs","volume":"77","author":"Silva","year":"2005","journal-title":"Anal. Chem."},{"key":"10.1016\/j.pep.2016.10.008_bib13","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1074\/mcp.M500321-MCP200","article-title":"Simultaneous qualitative and quantitative analysis of the Escherichia coli proteome: a sweet tale","volume":"5","author":"Silva","year":"2006","journal-title":"Mol. Cell Proteomics"},{"key":"10.1016\/j.pep.2016.10.008_bib14","doi-asserted-by":"crossref","first-page":"1696","DOI":"10.1002\/pmic.200800564","article-title":"Database searching and accounting of multiplexed precursor and product ion spectra from the data independent analysis of simple and complex peptide mixtures","volume":"9","author":"Li","year":"2009","journal-title":"Proteomics"},{"key":"10.1016\/j.pep.2016.10.008_bib15","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1111\/j.2042-7158.1955.tb12043.x","article-title":"The operating characteristics of some official weight variation tests for tablets","volume":"7","author":"Dunnett","year":"1955","journal-title":"J.\u00a0Pharm. Pharmacol."},{"key":"10.1016\/j.pep.2016.10.008_bib16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1756-3305-7-361","article-title":"A\u00a0canine model of experimental infection with Leishmania (L.) mexicana","volume":"7","author":"Cruz-Chan","year":"2014","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.pep.2016.10.008_bib17","doi-asserted-by":"crossref","first-page":"8185","DOI":"10.1016\/S0021-9258(19)86874-6","article-title":"Nucleosidases from Leishmania donovani. Pyrimidine ribonucleosidase, purine ribonucleosidase, and a novel purine 2'-deoxyribonucleosidase","volume":"254","author":"Koszalka","year":"1979","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.pep.2016.10.008_bib18","doi-asserted-by":"crossref","first-page":"3104","DOI":"10.1016\/S0264-410X(01)00031-7","article-title":"Vaccination of Balb\/c mice against experimental visceral leishmaniasis with the GP36 glycoprotein antigen of Leishmania donovani","volume":"19","author":"Paraguai de Souza","year":"2001","journal-title":"Vaccine"}],"container-title":["Protein Expression and Purification"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592816303370?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592816303370?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,7,10]],"date-time":"2022-07-10T17:26:20Z","timestamp":1657473980000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046592816303370"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2]]},"references-count":18,"alternative-id":["S1046592816303370"],"URL":"https:\/\/doi.org\/10.1016\/j.pep.2016.10.008","relation":{},"ISSN":["1046-5928"],"issn-type":[{"value":"1046-5928","type":"print"}],"subject":[],"published":{"date-parts":[[2017,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Expression, purification, immunogenicity and protective efficacy of a recombinant nucleoside hydrolase from Leishmania donovani, a vaccine candidate for preventing cutaneous leishmaniasis","name":"articletitle","label":"Article Title"},{"value":"Protein Expression and Purification","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.pep.2016.10.008","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}