{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T13:50:35Z","timestamp":1790085035912,"version":"4.0.1"},"reference-count":30,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1R15 GM131289"],"award-info":[{"award-number":["1R15 GM131289"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Protein Expression and Purification"],"published-print":{"date-parts":[[2021,2]]},"DOI":"10.1016\/j.pep.2020.105778","type":"journal-article","created":{"date-parts":[[2020,10,15]],"date-time":"2020-10-15T08:34:18Z","timestamp":1602750858000},"page":"105778","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Production of a human mitochondrial ABC transporter in E. coli"],"prefix":"10.1016","volume":"178","author":[{"given":"Alexandra D.","family":"Saxberg","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Martinez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory A.","family":"Fendley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria E.","family":"Zoghbi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.pep.2020.105778_bib1","doi-asserted-by":"crossref","first-page":"235","DOI":"10.33549\/physiolres.930432","article-title":"ATP-binding cassette (ABC) transporters in human metabolism and diseases","volume":"53","author":"Stefkova","year":"2004","journal-title":"Physiol. Res."},{"issue":"7","key":"10.1016\/j.pep.2020.105778_bib2","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.1101\/gr.184901","article-title":"The human ATP-binding cassette (ABC) transporter superfamily","volume":"11","author":"Dean","year":"2001","journal-title":"Genome Res."},{"issue":"3","key":"10.1016\/j.pep.2020.105778_bib3","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1186\/1479-7364-3-3-281","article-title":"Human ATP-binding cassette (ABC) transporter family","volume":"3","author":"Vasiliou","year":"2009","journal-title":"Hum. Genom."},{"key":"10.1016\/j.pep.2020.105778_bib4","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.sbi.2018.03.016","article-title":"Multifaceted structures and mechanisms of ABC transport systems in health and disease","volume":"51","author":"Thomas","year":"2018","journal-title":"Curr. Opin. Struct. Biol."},{"issue":"1","key":"10.1016\/j.pep.2020.105778_bib5","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1042\/BST20180147","article-title":"Learning the ABCs one at a time: structure and mechanism of ABC transporters","volume":"47","author":"Ford","year":"2019","journal-title":"Biochem. Soc. Trans."},{"issue":"6","key":"10.1016\/j.pep.2020.105778_bib6","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1038\/nsmb.3216","article-title":"Mechanistic diversity in ATP-binding cassette (ABC) transporters","volume":"23","author":"Locher","year":"2016","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.pep.2020.105778_bib7","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.pep.2019.03.002","article-title":"Large-scale purification of functional human P-glycoprotein (ABCB1)","volume":"159","author":"Nandigama","year":"2019","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.pep.2020.105778_bib8","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.pep.2014.02.010","article-title":"Expression, purification and structural properties of ABC transporter ABCA4 and its individual domains","volume":"97","author":"Tsybovsky","year":"2014","journal-title":"Protein Expr. Purif."},{"issue":"1 Pt B","key":"10.1016\/j.pep.2020.105778_bib9","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.bbamem.2013.09.001","article-title":"Improving the stability and function of purified ABCB1 and ABCA4: the influence of membrane lipids","volume":"1838","author":"Pollock","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"issue":"7587","key":"10.1016\/j.pep.2020.105778_bib10","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1038\/nature16506","article-title":"A mechanism of viral immune evasion revealed by cryo-EM analysis of the TAP transporter","volume":"529","author":"Oldham","year":"2016","journal-title":"Nature"},{"issue":"16","key":"10.1016\/j.pep.2020.105778_bib11","doi-asserted-by":"crossref","first-page":"3403","DOI":"10.1021\/bi902064g","article-title":"Bile acids stimulate ATP hydrolysis in the purified cholesterol transporter ABCG5\/G8","volume":"49","author":"Johnson","year":"2010","journal-title":"Biochemistry"},{"issue":"4","key":"10.1016\/j.pep.2020.105778_bib12","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0060620","article-title":"Detergent screening and purification of the human liver ABC transporters BSEP (ABCB11) and MDR3 (ABCB4) expressed in the yeast Pichia pastoris","volume":"8","author":"Ellinger","year":"2013","journal-title":"PloS One"},{"key":"10.1016\/j.pep.2020.105778_bib13","article-title":"Purification of the cystic fibrosis transmembrane conductance regulator protein expressed in Saccharomyces cerevisiae","volume":"87","author":"Pollock","year":"2014","journal-title":"JoVE"},{"issue":"24","key":"10.1016\/j.pep.2020.105778_bib14","doi-asserted-by":"crossref","first-page":"9710","DOI":"10.1073\/pnas.1217042110","article-title":"Structures of ABCB10, a human ATP-binding cassette transporter in apo- and nucleotide-bound states","volume":"110","author":"Shintre","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"7731","key":"10.1016\/j.pep.2020.105778_bib15","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1038\/s41586-018-0680-3","article-title":"Cryo-EM structures of a human ABCG2 mutant trapped in ATP-bound and substrate-bound states","volume":"563","author":"Manolaridis","year":"2018","journal-title":"Nature"},{"issue":"2","key":"10.1016\/j.pep.2020.105778_bib16","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1042\/BJ20121485","article-title":"Effects of the lipid environment, cholesterol and bile acids on the function of the purified and reconstituted human ABCG2 protein","volume":"450","author":"Telbisz","year":"2013","journal-title":"Biochem. J."},{"issue":"8","key":"10.1016\/j.pep.2020.105778_bib17","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1016\/j.bbamcr.2013.10.023","article-title":"Bacterial-based membrane protein production","volume":"1843","author":"Schlegel","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"issue":"23","key":"10.1016\/j.pep.2020.105778_bib18","doi-asserted-by":"crossref","first-page":"20645","DOI":"10.1074\/jbc.M301358200","article-title":"Sterol transport by the human breast cancer resistance protein (ABCG2) expressed in Lactococcus lactis","volume":"278","author":"Janvilisri","year":"2003","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.pep.2020.105778_bib19","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/978-1-60761-762-4_4","article-title":"Insect cell versus bacterial overexpressed membrane proteins: an example, the human ABCG2 transporter","volume":"654","author":"Pozza","year":"2010","journal-title":"Methods Mol. Biol."},{"issue":"11","key":"10.1016\/j.pep.2020.105778_bib20","doi-asserted-by":"crossref","first-page":"2492","DOI":"10.1093\/emboj\/19.11.2492","article-title":"ABC-me: a novel mitochondrial transporter induced by GATA-1 during erythroid differentiation","volume":"19","author":"Shirihai","year":"2000","journal-title":"EMBO J."},{"issue":"41","key":"10.1016\/j.pep.2020.105778_bib21","doi-asserted-by":"crossref","first-page":"42954","DOI":"10.1074\/jbc.M405040200","article-title":"Targeting, import, and dimerization of a mammalian mitochondrial ATP binding cassette (ABC) transporter, ABCB10 (ABC-me)","volume":"279","author":"Graf","year":"2004","journal-title":"J. Biol. Chem."},{"issue":"5","key":"10.1016\/j.pep.2020.105778_bib22","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1042\/BST20150118","article-title":"Structures and functions of mitochondrial ABC transporters","volume":"43","author":"Schaedler","year":"2015","journal-title":"Biochem. Soc. Trans."},{"issue":"10","key":"10.1016\/j.pep.2020.105778_bib23","doi-asserted-by":"crossref","first-page":"1945","DOI":"10.1016\/j.bbamcr.2012.07.013","article-title":"Mitochondrial ABC transporters function: the role of ABCB10 (ABC-me) as a novel player in cellular handling of reactive oxygen species","volume":"1823","author":"Liesa","year":"2012","journal-title":"Biochim. Biophys. Acta"},{"issue":"7","key":"10.1016\/j.pep.2020.105778_bib24","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1038\/cdd.2011.195","article-title":"The mitochondrial transporter ABC-me (ABCB10), a downstream target of GATA-1, is essential for erythropoiesis in vivo","volume":"19","author":"Hyde","year":"2012","journal-title":"Cell Death Differ."},{"issue":"6","key":"10.1016\/j.pep.2020.105778_bib25","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1128\/MCB.00865-13","article-title":"Abcb10 role in heme biosynthesis in vivo: Abcb10 knockout in mice causes anemia with protoporphyrin IX and iron accumulation","volume":"34","author":"Yamamoto","year":"2014","journal-title":"Mol. Cell Biol."},{"key":"10.1016\/j.pep.2020.105778_bib26","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/S0076-6879(09)64011-8","article-title":"Chapter 11 - reconstitution of membrane proteins in phospholipid bilayer nanodiscs","volume":"464","author":"Ritchie","year":"2009","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.pep.2020.105778_bib27","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/978-1-4939-3179-8_11","article-title":"ATPase activity measurements by an enzyme-coupled spectrophotometric assay","volume":"1377","author":"Sehgal","year":"2016","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.pep.2020.105778_bib28","doi-asserted-by":"crossref","first-page":"4453","DOI":"10.1074\/jbc.M115.698498","article-title":"The lipid bilayer modulates the structure and function of an ATP-binding cassette exporter","volume":"291","author":"Zoghbi","year":"2016","journal-title":"J. Biol. Chem."},{"issue":"50","key":"10.1016\/j.pep.2020.105778_bib29","doi-asserted-by":"crossref","first-page":"20412","DOI":"10.1074\/jbc.M117.814186","article-title":"Substrate-induced conformational changes in the nucleotide-binding domains of lipid bilayer-associated P-glycoprotein during ATP hydrolysis","volume":"292","author":"Zoghbi","year":"2017","journal-title":"J. Biol. Chem."},{"issue":"1","key":"10.1016\/j.pep.2020.105778_bib30","first-page":"63","article-title":"Catalytic and transport cycles of ABC exporters","volume":"50","author":"Al-Shawi","year":"2011","journal-title":"Essays Biochem."}],"container-title":["Protein Expression and Purification"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592820303697?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592820303697?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T01:54:19Z","timestamp":1760925259000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046592820303697"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2]]},"references-count":30,"alternative-id":["S1046592820303697"],"URL":"https:\/\/doi.org\/10.1016\/j.pep.2020.105778","relation":{},"ISSN":["1046-5928"],"issn-type":[{"value":"1046-5928","type":"print"}],"subject":[],"published":{"date-parts":[[2021,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Production of a human mitochondrial ABC transporter in E. coli","name":"articletitle","label":"Article Title"},{"value":"Protein Expression and Purification","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.pep.2020.105778","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"105778"}}