{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T12:37:58Z","timestamp":1777725478975,"version":"3.51.4"},"reference-count":32,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100012542","name":"Sichuan Province Science and Technology Support Program","doi-asserted-by":"publisher","award":["2019YFG0486"],"award-info":[{"award-number":["2019YFG0486"]}],"id":[{"id":"10.13039\/100012542","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016809","name":"Bureau of Science and Technology Nanchong City","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Protein Expression and Purification"],"published-print":{"date-parts":[[2022,1]]},"DOI":"10.1016\/j.pep.2021.105966","type":"journal-article","created":{"date-parts":[[2021,10,9]],"date-time":"2021-10-09T03:21:36Z","timestamp":1633749696000},"page":"105966","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["Preparation and characterization of a high-af\ufb01nity monoclonal antibody against nerve growth factor"],"prefix":"10.1016","volume":"189","author":[{"given":"Shuang","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9845-9175","authenticated-orcid":false,"given":"Yunlong","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengyu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cuiyu","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangbing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohua","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianxiong","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinliang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.pep.2021.105966_bib1","doi-asserted-by":"crossref","first-page":"10657","DOI":"10.3390\/molecules200610657","article-title":"Neurotrophins and neuropathic pain: role in pathobiology","volume":"2","author":"Khan","year":"2015","journal-title":"Molecules"},{"key":"10.1016\/j.pep.2021.105966_bib2","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/0304-3959(95)00024-M","article-title":"Peripheral administration of nerve growth factor in the adult rat produces a thermal hyperalgesia that requires the presence of sympathetic post-ganglionic neurones","volume":"63","author":"Andreev","year":"1995","journal-title":"Pain"},{"key":"10.1016\/j.pep.2021.105966_bib3","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1212\/WNL.48.2.501","article-title":"Intradermal recombinant human nerve growth factor induces pressure allodynia and lowered heat-pain threshold in humans","volume":"48","author":"Dyck","year":"1997","journal-title":"Neurology"},{"key":"10.1016\/j.pep.2021.105966_bib4","doi-asserted-by":"crossref","first-page":"294","DOI":"10.2174\/1570159X13666150403231920","article-title":"Nerve growth factor: a focus on neuroscience and therapy","volume":"13","author":"Aloe","year":"2015","journal-title":"Curr. Neuropharmacol."},{"key":"10.1016\/j.pep.2021.105966_bib5","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1146\/annurev.neuro.24.1.1217","article-title":"Nerve growth factor signaling, neuroprotection, and neural repair","volume":"24","author":"Sofroniew","year":"2001","journal-title":"Annu. Rev. Neurosci."},{"key":"10.1016\/j.pep.2021.105966_bib6","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1186\/1479-5876-10-239","article-title":"Nerve growth factor: from the early discoveries to the potential clinical use","volume":"10","author":"Aloe","year":"2012","journal-title":"J. Transl. Med."},{"key":"10.1016\/j.pep.2021.105966_bib7","first-page":"759473","article-title":"The nerve growth factor signaling and its potential as therapeutic target for glaucoma","author":"Wang","year":"2014","journal-title":"BioMed Res. Int."},{"key":"10.1016\/j.pep.2021.105966_bib8","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/S0166-2236(96)10058-8","article-title":"Nerve growth factor: from neurotrophin to neurokine","volume":"19","author":"Levi-Montalcini","year":"1996","journal-title":"Trends Neurosci."},{"key":"10.1016\/j.pep.2021.105966_bib9","first-page":"68","article-title":"The role of nerve growth factor and its receptors in tumorigenesis and cancer pain, Biosci","volume":"8","author":"Wang","year":"2014","journal-title":"Trends"},{"key":"10.1016\/j.pep.2021.105966_bib10","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1126\/science.1850549","article-title":"The trk proto-oncogene product: a signal transducing receptor for nerve growth factor","volume":"252","author":"Kaplan","year":"1991","journal-title":"Science"},{"key":"10.1016\/j.pep.2021.105966_bib11","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1152\/physiol.00042.2004","article-title":"Neurotrophin-mediated rapid signaling in the central nervous system: mechanisms and functions","volume":"20","author":"Blum","year":"2005","journal-title":"Physiology"},{"key":"10.1016\/j.pep.2021.105966_bib12","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/j.pain.2009.11.022","article-title":"NGF induces non-inflammatory localized and lasting mechanical and thermal hypersensitivity in human skin","volume":"148","author":"Rukwied","year":"2010","journal-title":"Pain"},{"key":"10.1016\/j.pep.2021.105966_bib13","doi-asserted-by":"crossref","first-page":"599","DOI":"10.3390\/ijms18030599","article-title":"ProNGF, but not NGF, switches from neurotrophic to apoptotic activity in response to reductions in TrKA receptor levels","volume":"18","author":"Ioannou","year":"2017","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.pep.2021.105966_bib14","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.tips.2005.12.001","article-title":"Novel class of pain drugs based on antagonism of NGF","volume":"27","author":"Hefti","year":"2006","journal-title":"Trends Pharmacol. Sci."},{"key":"10.1016\/j.pep.2021.105966_bib15","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.3390\/ijms18051028","article-title":"NGF and its receptors in the regulation of inflammatory response","volume":"18","author":"Minnone","year":"2017","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.pep.2021.105966_bib16","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1097\/ALN.0b013e31821b1ac5","article-title":"Antagonism of nerve growth factor-TrkA signaling and the relief of pain","volume":"115","author":"Mantyh","year":"2011","journal-title":"Anesthesiology"},{"key":"10.1016\/j.pep.2021.105966_bib17","first-page":"129","article-title":"NGF in early embryogenesis, differentiation, and pathology in the nervous and immune systems","volume":"29","author":"Bracci-Laudiero","year":"2016","journal-title":"Curr. Top. Behav. Neurosci."},{"key":"10.1016\/j.pep.2021.105966_bib18","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1038\/nm0895-774","article-title":"The biological effects of endogenous nerve growth factor on adult sensory neurons revealed by a trka-igg fusion molecule","volume":"1","author":"McMahon","year":"1995","journal-title":"Nat. Med."},{"key":"10.1016\/j.pep.2021.105966_bib19","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/S0165-5728(97)00055-6","article-title":"Distribution of NGF receptors in normal and pathologic human lymphoid tissues","volume":"77","author":"Labouyrie","year":"1997","journal-title":"J. Neuroimmunol."},{"key":"10.1016\/j.pep.2021.105966_bib20","first-page":"94","article-title":"Tanezumab, a recombinant humanized mAb against nerve growth factor for the treatment of acute and chronic pain","volume":"12","author":"Cattaneo","year":"2010","journal-title":"Curr. Opin. Mol. Therapeut."},{"key":"10.1016\/j.pep.2021.105966_bib21","doi-asserted-by":"crossref","first-page":"457","DOI":"10.2147\/JPR.S105892","article-title":"A review of chronic pain impact on patients, their social environment and the health care system","volume":"28","author":"Due\u00f1as","year":"2016","journal-title":"J. Pain Res."},{"key":"10.1016\/j.pep.2021.105966_bib22","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.bja.2019.03.023","article-title":"Chronic pain: a review of its epidemiology and associated factors in population-based studies","volume":"123","author":"Mills","year":"2019","journal-title":"Br. J. Anaesth."},{"key":"10.1016\/j.pep.2021.105966_bib23","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1111\/jnc.12093","article-title":"Nerve growth factor-mediated regulation of pain signalling and proposed new intervention strategies in clinical pain management","volume":"124","author":"McKelvey","year":"2013","journal-title":"J. Neurochem."},{"key":"10.1016\/j.pep.2021.105966_bib24","doi-asserted-by":"crossref","first-page":"349","DOI":"10.2165\/0063030-200822060-00002","article-title":"Targeting nerve growth factor in pain: what is the therapeutic potential?","volume":"22","author":"Watson","year":"2008","journal-title":"BioDrugs"},{"key":"10.1016\/j.pep.2021.105966_bib25","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/B978-0-7020-4086-3.00038-2","article-title":"Nonsteroidal anti-inflammatory drugs exposure and the central nervous system","volume":"119","author":"Auriel","year":"2014","journal-title":"Handb. Clin. Neurol."},{"key":"10.1016\/j.pep.2021.105966_bib26","doi-asserted-by":"crossref","first-page":"279","DOI":"10.2147\/JPR.S33408","article-title":"NGF - the TrkA to successful pain treatment","volume":"5","author":"Kumar","year":"2012","journal-title":"J. Pain Res."},{"key":"10.1016\/j.pep.2021.105966_bib27","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.jbior.2014.11.003","article-title":"NGF and ProNGF: regulation of neuronal and neoplastic responses through receptor signaling","volume":"58","author":"Bradshaw","year":"2015","journal-title":"Adv. Biol. Regul."},{"key":"10.1016\/j.pep.2021.105966_bib28","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1124\/jpet.106.116236","article-title":"Antibodies to nerve growth factor reverse established tactile allodynia in rodent models of neuropathic pain without tolerance","volume":"322","author":"Wild","year":"2007","journal-title":"J. Pharmacol. Exp. Therapeut."},{"key":"10.1016\/j.pep.2021.105966_bib29","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1016\/j.neuroscience.2010.08.056","article-title":"Blockade of nerve sprouting and neuroma formation markedly attenuates the development of late stage cancer pain","volume":"171","author":"Mantyh","year":"2010","journal-title":"Neuroscience"},{"key":"10.1016\/j.pep.2021.105966_bib30","doi-asserted-by":"crossref","first-page":"11756","DOI":"10.1523\/JNEUROSCI.1960-11.2011","article-title":"APP is Phosphorylated by TrkA and regulates NGF\/TrkA signaling","volume":"31","author":"Matrone","year":"2011","journal-title":"J. Neurosci."},{"key":"10.1016\/j.pep.2021.105966_bib31","doi-asserted-by":"crossref","first-page":"3243","DOI":"10.1002\/art.30564","article-title":"Nerve growth factor: a key local regulator in the pathogenesis of inflammatory arthritis","volume":"63","author":"Raychaudhuri","year":"2011","journal-title":"Arthritis Rheum."},{"key":"10.1016\/j.pep.2021.105966_bib32","doi-asserted-by":"crossref","first-page":"3345","DOI":"10.4049\/jimmunol.1300825","article-title":"Nerve growth factor downregulates inflammatory response in human monocytes through TrkA","volume":"192","author":"Prencipe","year":"2014","journal-title":"J. Immunol."}],"container-title":["Protein Expression and Purification"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592821001492?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592821001492?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T01:55:52Z","timestamp":1760925352000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046592821001492"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1]]},"references-count":32,"alternative-id":["S1046592821001492"],"URL":"https:\/\/doi.org\/10.1016\/j.pep.2021.105966","relation":{},"ISSN":["1046-5928"],"issn-type":[{"value":"1046-5928","type":"print"}],"subject":[],"published":{"date-parts":[[2022,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Preparation and characterization of a high-af\ufb01nity monoclonal antibody against nerve growth factor","name":"articletitle","label":"Article Title"},{"value":"Protein Expression and Purification","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.pep.2021.105966","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"105966"}}