{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T22:11:53Z","timestamp":1778710313076,"version":"3.51.4"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T00:00:00Z","timestamp":1709251200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T00:00:00Z","timestamp":1709251200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T00:00:00Z","timestamp":1709251200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T00:00:00Z","timestamp":1709251200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T00:00:00Z","timestamp":1709251200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T00:00:00Z","timestamp":1709251200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T00:00:00Z","timestamp":1709251200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Protein Expression and Purification"],"published-print":{"date-parts":[[2024,3]]},"DOI":"10.1016\/j.pep.2023.106402","type":"journal-article","created":{"date-parts":[[2023,11,11]],"date-time":"2023-11-11T11:47:20Z","timestamp":1699703240000},"page":"106402","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Enzymatic and antibacterial activity of the recombinant endolysin PVP-SE1gp146 expressed in Hansenula polymorpha"],"prefix":"10.1016","volume":"215","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4851-8311","authenticated-orcid":false,"given":"Abolghasem","family":"Asadi-Saghandi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5479-2846","authenticated-orcid":false,"given":"Mojgan","family":"Bandehpour","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Hashemi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bahram","family":"Kazemi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.pep.2023.106402_bib1","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.2147\/IDR.S173867","article-title":"Antibiotic resistance: a rundown of a global crisis","volume":"11","author":"Aslam","year":"2018","journal-title":"Infect. Drug Resist."},{"key":"10.1016\/j.pep.2023.106402_bib2","series-title":"Antibiotic Resistance, Treasure Island (FL)","author":"Habboush","year":"2022"},{"key":"10.1016\/j.pep.2023.106402_bib3","doi-asserted-by":"crossref","DOI":"10.3390\/v13040680","article-title":"The advantages and challenges of using endolysins in a clinical setting","volume":"13","author":"Murray","year":"2021","journal-title":"Viruses"},{"key":"10.1016\/j.pep.2023.106402_bib4","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1007\/978-981-13-7709-9_11","article-title":"Enzybiotics: enzyme-based antibacterials as therapeutics","volume":"1148","author":"Dams","year":"2019","journal-title":"Adv. Exp. Med. Biol."},{"key":"10.1016\/j.pep.2023.106402_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/j.pep.2020.105676","article-title":"High cell-density fermentation, expression and purification of bacteriophage lysin TSPphg, a thermostable antimicrobial protein from extremophilic Thermus bacteriophage TSP4","volume":"174","author":"Wang","year":"2020","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.pep.2023.106402_bib6","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1021\/acssynbio.9b00360","article-title":"Yeast surface display system for facilitated production and application of phage endolysin","volume":"9","author":"Chun","year":"2020","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.pep.2023.106402_bib7","doi-asserted-by":"crossref","DOI":"10.3390\/v10060281","article-title":"Yeast derived LysA2 can control bacterial contamination in ethanol fermentation","volume":"10","author":"Kim","year":"2018","journal-title":"Viruses"},{"key":"10.1016\/j.pep.2023.106402_bib8","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1007\/s00299-019-02459-1","article-title":"Production of bacteriophage-encoded endolysin, LysP11, in Nicotiana benthamiana and its activity as a potent antimicrobial agent against Erysipelothrix rhusiopathiae","volume":"38","author":"Islam","year":"2019","journal-title":"Plant Cell Rep."},{"key":"10.1016\/j.pep.2023.106402_bib9","doi-asserted-by":"crossref","DOI":"10.1155\/2019\/4216060","article-title":"A brief reminder of systems of production and chromatography-based recovery of recombinant protein biopharmaceuticals","volume":"2019","author":"Owczarek","year":"2019","journal-title":"BioMed Res. Int."},{"key":"10.1016\/j.pep.2023.106402_bib10","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1007\/s00253-004-1656-9","article-title":"Recombinant expression systems in the pharmaceutical industry","volume":"65","author":"Schmidt","year":"2004","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.pep.2023.106402_bib11","doi-asserted-by":"crossref","first-page":"94","DOI":"10.3389\/fbioe.2019.00094","article-title":"Advances in using Hansenula polymorpha as chassis for recombinant protein production","volume":"7","author":"Manfrao-Netto","year":"2019","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.pep.2023.106402_bib12","first-page":"607","article-title":"Bacillus phage endolysin, lys46, bactericidal properties against gram-negative bacteria","volume":"12","author":"Sarjoughian","year":"2020","journal-title":"Iran. J. Microbiol."},{"key":"10.1016\/j.pep.2023.106402_bib13","article-title":"Development of a novel chimeric endolysin, Lys109 with enhanced lytic activity against Staphylococcus aureus","volume":"11","author":"Son","year":"2020","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.pep.2023.106402_bib14","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2022.841905","article-title":"Direct lytic agents: novel, rapidly acting potential antimicrobial treatment modalities for systemic use in the era of rising antibiotic resistance","volume":"13","author":"Schuch","year":"2022","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.pep.2023.106402_bib15","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0036991","article-title":"Characterization of modular bacteriophage endolysins from Myoviridae phages OBP, 201phi2-1 and PVP-SE1","volume":"7","author":"Walmagh","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.pep.2023.106402_bib16","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.01379-14","article-title":"Engineered endolysin-based \"Artilysins\" to combat multidrug-resistant gram-negative pathogens","volume":"5","author":"Briers","year":"2014","journal-title":"mBio"},{"key":"10.1016\/j.pep.2023.106402_bib17","first-page":"253","article-title":"Transformation of plasmid DNA into E. coli using the heat shock method","author":"Froger","year":"2007","journal-title":"J. Vis. Exp."},{"key":"10.1016\/j.pep.2023.106402_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.pep.2020.105685","article-title":"Modified chemical method for efficient transformation and diagnosis in Pichia pastoris","volume":"174","author":"Kumar","year":"2020","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.pep.2023.106402_bib19","doi-asserted-by":"crossref","first-page":"e749","DOI":"10.1002\/cpz1.749","article-title":"Modified phenol\/chloroform\u2010free DNA isolation from yeast and other fungi by non\u2010enzymatic salting out method","volume":"3","author":"Bandehpour","year":"2023","journal-title":"Current protocols"},{"key":"10.1016\/j.pep.2023.106402_bib20","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1007\/BF00446560","article-title":"Growth of Hansenula polymorpha in a methanol-limited chemostat","volume":"111","author":"Van Dijken","year":"1976","journal-title":"Arch. Microbiol."},{"key":"10.1016\/j.pep.2023.106402_bib21","article-title":"Spectrophotometric and colorimetric determination of protein concentration","volume":"10","author":"Simonian","year":"2006","journal-title":"Curr Protoc Mol Biol Chapter"},{"key":"10.1016\/j.pep.2023.106402_bib22","article-title":"Spectrophotometric determination of protein concentration","volume":"3","author":"Grimsley","year":"2004","journal-title":"Curr Protoc Protein Sci Chapter"},{"key":"10.1016\/j.pep.2023.106402_bib23","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1038\/s41589-021-00948-7","article-title":"Convergent evolution of bacterial ceramide synthesis","volume":"18","author":"Stankeviciute","year":"2022","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.pep.2023.106402_bib24","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/j.jbbm.2006.10.009","article-title":"A standardized approach for accurate quantification of murein hydrolase activity in high-throughput assays","volume":"70","author":"Briers","year":"2007","journal-title":"J. Biochem. Biophys. Methods"},{"key":"10.1016\/j.pep.2023.106402_bib25","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.foodcont.2005.08.010","article-title":"Antibacterial effect of phenolic compounds from different wines","volume":"18","author":"Vaquero","year":"2007","journal-title":"Food Control"},{"key":"10.1016\/j.pep.2023.106402_bib26","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1128\/mr.56.3.395-411.1992","article-title":"Agents that increase the permeability of the outer membrane","volume":"56","author":"Vaara","year":"1992","journal-title":"Microbiol. Rev."},{"key":"10.1016\/j.pep.2023.106402_bib27","article-title":"Endolysin, a promising solution against antimicrobial resistance","volume":"10","author":"Rahman","year":"2021","journal-title":"Antibiotics (Basel)"},{"key":"10.1016\/j.pep.2023.106402_bib28","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.2217\/fmb.12.97","article-title":"Bacteriophage endolysins as novel antimicrobials","volume":"7","author":"Schmelcher","year":"2012","journal-title":"Future Microbiol."},{"key":"10.1016\/j.pep.2023.106402_bib29","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/s12929-023-00919-1","article-title":"Therapeutic potential of bacteriophage endolysins for infections caused by Gram-positive bacteria","volume":"30","author":"Liu","year":"2023","journal-title":"J. Biomed. Sci."},{"key":"10.1016\/j.pep.2023.106402_bib30","article-title":"Potential applications of thermophilic bacteriophages in one health","volume":"24","author":"Liu","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.pep.2023.106402_bib31","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/s12934-022-01766-9","article-title":"Evaluation of factors influencing expression and extraction of recombinant bacteriophage endolysins in Escherichia coli","volume":"21","author":"Balaban","year":"2022","journal-title":"Microb. Cell Factories"},{"key":"10.1016\/j.pep.2023.106402_bib32","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1186\/s12896-019-0594-7","article-title":"Optimized production of a biologically active Clostridium perfringens glycosyl hydrolase phage endolysin PlyCP41 in plants using virus-based systemic expression","volume":"19","author":"Hammond","year":"2019","journal-title":"BMC Biotechnol."},{"key":"10.1016\/j.pep.2023.106402_bib33","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1007\/s00253-016-7822-z","article-title":"Expression of prophage-encoded endolysins contributes to autolysis of Lactococcus lactis","volume":"101","author":"Visweswaran","year":"2017","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.pep.2023.106402_bib34","article-title":"Current status of endolysin-based treatments against gram-negative bacteria","volume":"10","author":"Gontijo","year":"2021","journal-title":"Antibiotics (Basel)"},{"key":"10.1016\/j.pep.2023.106402_bib35","doi-asserted-by":"crossref","DOI":"10.3390\/ijms23147612","article-title":"Cloning and characterization of a thermostable endolysin of bacteriophage TP-84 as a potential disinfectant and biofilm-removing biological agent","volume":"23","author":"\u017bebrowska","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.pep.2023.106402_bib36","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1007\/s00253-009-2248-5","article-title":"High-temperature fermentation: how can processes for ethanol production at high temperatures become superior to the traditional process using mesophilic yeast?","volume":"85","author":"Abdel-Banat","year":"2009","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.pep.2023.106402_bib37","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-017-14797-9","article-title":"Modular endolysin of Burkholderia AP3 phage has the largest lysozyme-like catalytic subunit discovered to date and no catalytic aspartate residue","volume":"7","author":"Maciejewska","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.pep.2023.106402_bib38","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/s12929-023-00919-1","article-title":"Therapeutic potential of bacteriophage endolysins for infections caused by Gram-positive bacteria","volume":"30","author":"Liu","year":"2023","journal-title":"J. Biomed. Sci."},{"key":"10.1016\/j.pep.2023.106402_bib39","doi-asserted-by":"crossref","DOI":"10.1002\/0471140864.ps0601s80","article-title":"Overview of the purification of recombinant proteins","volume":"80","author":"Wingfield","year":"2015","journal-title":"Curr Protoc Protein Sci"},{"key":"10.1016\/j.pep.2023.106402_bib40","doi-asserted-by":"crossref","DOI":"10.3390\/ijms20020408","article-title":"The biophysical interaction of the danger-associated molecular pattern (DAMP) calreticulin with the pattern-associated molecular pattern (PAMP) lipopolysaccharide","volume":"20","author":"Pandya","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.pep.2023.106402_bib41","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2021.748718","article-title":"Discovering the potentials of four phage endolysins to combat gram-negative infections","volume":"12","author":"Vasina","year":"2021","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.pep.2023.106402_bib42","doi-asserted-by":"crossref","DOI":"10.3390\/v15020520","article-title":"Pseudomonas phage ZCPS1 endolysin as a potential therapeutic agent","volume":"15","author":"Abdelrahman","year":"2023","journal-title":"Viruses"},{"key":"10.1016\/j.pep.2023.106402_bib43","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2021.668430","article-title":"Phage endolysin LysP108 showed promising antibacterial potential against methicillin-resistant Staphylococcus aureus","volume":"11","author":"Lu","year":"2021","journal-title":"Front. Cell. Infect. Microbiol."}],"container-title":["Protein Expression and Purification"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592823001730?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592823001730?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T01:57:16Z","timestamp":1760925436000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046592823001730"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3]]},"references-count":43,"alternative-id":["S1046592823001730"],"URL":"https:\/\/doi.org\/10.1016\/j.pep.2023.106402","relation":{},"ISSN":["1046-5928"],"issn-type":[{"value":"1046-5928","type":"print"}],"subject":[],"published":{"date-parts":[[2024,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Enzymatic and antibacterial activity of the recombinant endolysin PVP-SE1gp146 expressed in Hansenula polymorpha","name":"articletitle","label":"Article Title"},{"value":"Protein Expression and Purification","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.pep.2023.106402","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"106402"}}