{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T10:56:26Z","timestamp":1775040986232,"version":"3.50.1"},"reference-count":14,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Protein Expression and Purification"],"published-print":{"date-parts":[[2024,4]]},"DOI":"10.1016\/j.pep.2024.106429","type":"journal-article","created":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T22:00:18Z","timestamp":1704492018000},"page":"106429","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Recombinant protein production and functional analysis of a M60-like-2 metallopeptidase enzyme from the carcinogenic liver fluke Opisthorchis viverrini"],"prefix":"10.1016","volume":"216","author":[{"given":"Jiraporn","family":"Jumpajan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sujittra","family":"Chaiyadet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prasert","family":"Saichua","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sirikachorn","family":"Tangkawatana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krajang","family":"Talabnin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thewarach","family":"Laha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sutas","family":"Suttiprapa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.pep.2024.106429_bib1","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1038\/nrgastro.2013.35","article-title":"The gastrointestinal mucus system in health and disease","volume":"10","author":"Johansson","year":"2013","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"10.1016\/j.pep.2024.106429_bib2","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1038\/nrmicro2538","article-title":"Mucin dynamics and enteric pathogens","volume":"9","author":"McGuckin","year":"2011","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.pep.2024.106429_bib3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0030287","article-title":"A novel extracellular metallopeptidase domain shared by animal Host-Associated mutualistic and pathogenic microbes","volume":"7","author":"Nakjang","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.pep.2024.106429_bib4","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1016\/j.parint.2010.09.008","article-title":"Opisthorchis viverrini excretory\/secretory products induce toll-like receptor 4 upregulation and production of interleukin 6 and 8 in cholangiocyte","volume":"59","author":"Ninlawan","year":"2010","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.pep.2024.106429_bib5","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1093\/glycob\/cwp048","article-title":"A complex , but uniform O -glycosylation of the human MUC2 mucin from colonic biopsies analyzed by nanoLC\/MS n","volume":"19","author":"Holm","year":"2009","journal-title":"Glycobiology"},{"key":"10.1016\/j.pep.2024.106429_bib6","series-title":"R: A Language and Environment for Statistical Computing","year":"2022"},{"key":"10.1016\/j.pep.2024.106429_bib7","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1136\/gut.17.12.971","article-title":"Diurnal variations in the pH of pathological gallbladder bile","volume":"17","author":"Sutur","year":"1976","journal-title":"Gut"},{"key":"10.1016\/j.pep.2024.106429_bib8","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1002\/j.2040-4603.2013.tb00519.x","article-title":"Bile formation and secretion","volume":"3","author":"Boyer","year":"2013","journal-title":"Compr. Physiol."},{"key":"10.1016\/j.pep.2024.106429_bib9","first-page":"183","article-title":"Intraluminal pH of the human gastrointestinal tract","volume":"46","author":"Fallingborg","year":"1999","journal-title":"Dan. Med. Bull."},{"key":"10.1016\/j.pep.2024.106429_bib10","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1016\/j.ijbiomac.2019.07.108","article-title":"N-glycans of bovine submaxillary mucin contain core-fucosylated and sulfated glycans but not sialylated glycans","volume":"138","author":"Kim","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"issue":"4","key":"10.1016\/j.pep.2024.106429_bib11","doi-asserted-by":"crossref","DOI":"10.3390\/biom10040636","article-title":"Structural and quantitative characterization of mucin-type O-glycans and the identification of O-glycosylation sites in bovine submaxillary mucin","volume":"10","author":"Kim","year":"2020","journal-title":"Biomolecules"},{"key":"10.1016\/j.pep.2024.106429_bib12","doi-asserted-by":"crossref","first-page":"4572","DOI":"10.1096\/fj.13-232751","article-title":"Infection with the carcinogenic liver fluke Opisthorchis viverrini modifies intestinal and biliary microbiome","volume":"27","author":"Plieskatt","year":"2013","journal-title":"Faseb. J."},{"key":"10.1016\/j.pep.2024.106429_bib13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pntd.0011111","article-title":"Opisthorchis viverrini , Clonorchis sinensis and Opisthorchis felineus liver flukes affect mammalian host microbiome in a species- specific manner","volume":"17","author":"Pakharukova","year":"2023","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.pep.2024.106429_bib14","article-title":"Identification, recombinant protein production, and functional analysis of a M60-like metallopeptidase, secreted by the liver fluke Opisthorchis viverrini","volume":"75","author":"Btt","year":"2020","journal-title":"Parasitol. Int."}],"container-title":["Protein Expression and Purification"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592824000019?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592824000019?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T01:57:39Z","timestamp":1760925459000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046592824000019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4]]},"references-count":14,"alternative-id":["S1046592824000019"],"URL":"https:\/\/doi.org\/10.1016\/j.pep.2024.106429","relation":{},"ISSN":["1046-5928"],"issn-type":[{"value":"1046-5928","type":"print"}],"subject":[],"published":{"date-parts":[[2024,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Recombinant protein production and functional analysis of a M60-like-2 metallopeptidase enzyme from the carcinogenic liver fluke Opisthorchis viverrini","name":"articletitle","label":"Article Title"},{"value":"Protein Expression and Purification","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.pep.2024.106429","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"106429"}}