{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:58:37Z","timestamp":1772121517539,"version":"3.50.1"},"reference-count":30,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100005031","name":"Bhabha Atomic Research Centre","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005031","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Protein Expression and Purification"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1016\/j.pep.2025.106789","type":"journal-article","created":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T22:12:50Z","timestamp":1754431970000},"page":"106789","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Recombinant expression and purification of Plasmodium heme detoxification protein in E. coli: Challenges and discoveries"],"prefix":"10.1016","volume":"236","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4871-3600","authenticated-orcid":false,"given":"Rahul","family":"Singh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ravindra D.","family":"Makde","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.pep.2025.106789_bib1","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/S0166-6851(01)00427-3","article-title":"Ginsburg, \u2018Intraerythrocytic Plasmodium falciparum utilizes only a fraction of the amino acids derived from the digestion of host cell cytosol for the biosynthesis of its proteins\u2019","volume":"119","author":"Krugliak","year":"2002","journal-title":"Mol. Biochem. Parasitol."},{"issue":"10","key":"10.1016\/j.pep.2025.106789_bib2","doi-asserted-by":"crossref","first-page":"4189","DOI":"10.1182\/blood-2002-08-2654","article-title":"Excess hemoglobin digestion and the osmotic stability of plasmodium falciparum-infected red blood cells","volume":"101","author":"Lew","year":"2003","journal-title":"Blood"},{"issue":"3","key":"10.1016\/j.pep.2025.106789_bib3","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1182\/blood.V100.3.879","article-title":"Pro-oxidant and cytotoxic effects of circulating heme","volume":"100","author":"Jeney","year":"2002","journal-title":"Blood"},{"key":"10.1016\/j.pep.2025.106789_bib4","doi-asserted-by":"crossref","DOI":"10.1155\/2011\/473709","article-title":"Heme Oxygenase-1: a critical link between iron metabolism, erythropoiesis, and development","volume":"2011","author":"Fraser","year":"2011","journal-title":"Adv Hematol"},{"issue":"13","key":"10.1016\/j.pep.2025.106789_bib5","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1016\/S0020-7519(02)00193-5","article-title":"Theories on malarial pigment formation and quinoline action","volume":"32","author":"Sullivan","year":"2002","journal-title":"Int. J. Parasitol."},{"issue":"11","key":"10.1016\/j.pep.2025.106789_bib6","doi-asserted-by":"crossref","first-page":"2649","DOI":"10.1021\/acs.accounts.1c00154","article-title":"Heme detoxification in the malaria parasite: a target for antimalarial drug development","volume":"54","author":"de Villiers","year":"2021","journal-title":"Acc. Chem. Res."},{"issue":"8","key":"10.1016\/j.pep.2025.106789_bib7","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1021\/acsinfecdis.3c00278","article-title":"In vitro investigation unveiling new insights into the antimalarial mechanism of chloroquine: role in perturbing nucleation events during heme to \u03b2-Hematin transformation","volume":"9","author":"Singh","year":"2023","journal-title":"ACS Infect. Dis."},{"issue":"6519","key":"10.1016\/j.pep.2025.106789_bib8","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1038\/374269a0","article-title":"Malarial haemozoin\/beta-haematin supports haem polymerization in the absence of protein","volume":"374","author":"Dorn","year":"1995","journal-title":"Nature"},{"issue":"1","key":"10.1016\/j.pep.2025.106789_bib9","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/0014-5793(94)00921-X","article-title":"Quinoline anti-malarial drugs inhibit spontaneous formation of beta-haematin (malaria pigment)","volume":"352","author":"Egan","year":"1994","journal-title":"FEBS Lett."},{"key":"10.1016\/j.pep.2025.106789_bib10","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1186\/1475-2875-11-337","article-title":"Neutral lipids associated with haemozoin mediate efficient and rapid \u03b2-haematin formation at physiological pH, temperature and ionic composition","volume":"11","author":"Ambele","year":"2012","journal-title":"Malar. J."},{"issue":"6553","key":"10.1016\/j.pep.2025.106789_bib11","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1038\/378138a0","article-title":"Haem polymerization in malaria","volume":"378","author":"Bendrat","year":"1995","journal-title":"Nature"},{"issue":"28","key":"10.1016\/j.pep.2025.106789_bib12","doi-asserted-by":"crossref","first-page":"11188","DOI":"10.1073\/pnas.1118120109","article-title":"Oriented nucleation of hemozoin at the digestive vacuole membrane in Plasmodium falciparum","volume":"109","author":"Kapishnikov","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"6356","key":"10.1016\/j.pep.2025.106789_bib13","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1038\/355167a0","article-title":"Inhibition by chloroquine of a novel haem polymerase enzyme activity in malaria trophozoites","volume":"355","author":"Slater","year":"1992","journal-title":"Nature"},{"issue":"5246","key":"10.1016\/j.pep.2025.106789_bib14","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1126\/science.271.5246.219","article-title":"Plasmodium hemozoin formation mediated by histidine-rich proteins","volume":"271","author":"Sullivan","year":"1996","journal-title":"Science"},{"issue":"4","key":"10.1016\/j.pep.2025.106789_bib15","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1000053","article-title":"HDP - a novel heme detoxification protein from the malaria parasite","volume":"4","author":"Jani","year":"2008","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.pep.2025.106789_bib16","doi-asserted-by":"crossref","first-page":"45","DOI":"10.12688\/wellcomeopenres.11896.2","article-title":"PhenoPlasm: a database of disruption phenotypes for malaria parasite genes","volume":"2","author":"Sanderson","year":"2017","journal-title":"Wellcome Open Res"},{"issue":"14","key":"10.1016\/j.pep.2025.106789_bib17","doi-asserted-by":"crossref","first-page":"5392","DOI":"10.1073\/pnas.1218412110","article-title":"Protein complex directs hemoglobin-to-hemozoin formation in Plasmodium falciparum","volume":"110","author":"Chugh","year":"2013","journal-title":"Proc. Natl. Acad. Sci."},{"issue":"7","key":"10.1016\/j.pep.2025.106789_bib18","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1093\/protein\/gzw015","article-title":"Consensus protein design","volume":"29","author":"Porebski","year":"2016","journal-title":"Protein Eng. Des. Sel."},{"issue":"6","key":"10.1016\/j.pep.2025.106789_bib19","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1093\/bioinformatics\/btu744","article-title":"DISOPRED3: precise disordered region predictions with annotated protein-binding activity","volume":"31","author":"Jones","year":"2015","journal-title":"Bioinformatics"},{"issue":"8016","key":"10.1016\/j.pep.2025.106789_bib20","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","article-title":"Accurate structure prediction of biomolecular interactions with AlphaFold 3","volume":"630","author":"Abramson","year":"2024","journal-title":"Nature"},{"issue":"W1","key":"10.1016\/j.pep.2025.106789_bib21","doi-asserted-by":"crossref","first-page":"W210","DOI":"10.1093\/nar\/gkac387","article-title":"Dali server: structural unification of protein families","volume":"50","author":"Holm","year":"2022","journal-title":"Nucleic Acids Res."},{"issue":"1","key":"10.1016\/j.pep.2025.106789_bib22","first-page":"343","article-title":"Atsas 3.0: expanded functionality and new tools for small-angle scattering data analysis","volume":"54","author":"Manalastas-Cantos","year":"2021"},{"issue":"5","key":"10.1016\/j.pep.2025.106789_bib23","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1107\/S1600576717011438","article-title":"BioXTAS RAW: improvements to a free open-source program for small-angle X-ray scattering data reduction and analysis","volume":"50","author":"Hopkins","year":"2017","journal-title":"J. Appl. Crystallogr."},{"issue":"6","key":"10.1016\/j.pep.2025.106789_bib24","first-page":"768","article-title":"Crysol \u2013 a program to evaluate X-ray solution scattering of biological macromolecules from atomic coordinates","volume":"28","author":"Svergun","year":"1995"},{"issue":"6","key":"10.1016\/j.pep.2025.106789_bib25","doi-asserted-by":"crossref","first-page":"2879","DOI":"10.1016\/S0006-3495(99)77443-6","article-title":"Restoring low resolution structure of biological macromolecules from solution scattering using simulated annealing","volume":"76","author":"Svergun","year":"1999","journal-title":"Biophys. J."},{"issue":"9","key":"10.1016\/j.pep.2025.106789_bib26","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbapap.2022.140832","article-title":"An assay procedure to investigate the transformation of toxic heme into inert hemozoin via plasmodial heme detoxification protein","volume":"1870","author":"Singh","year":"2022","journal-title":"Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics"},{"issue":"3","key":"10.1016\/j.pep.2025.106789_bib27","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1016\/j.jmb.2009.11.025","article-title":"Unusual diheme conformation of the heme-degrading protein from Mycobacterium tuberculosis","volume":"395","author":"Chim","year":"2010","journal-title":"J. Mol. Biol."},{"issue":"1","key":"10.1016\/j.pep.2025.106789_bib28","doi-asserted-by":"crossref","first-page":"6137","DOI":"10.1038\/srep06137","article-title":"Identification of essential histidine residues involved in heme binding and hemozoin formation in heme detoxification protein from Plasmodium falciparum","volume":"4","author":"Nakatani","year":"2014","journal-title":"Sci. Rep."},{"issue":"20","key":"10.1016\/j.pep.2025.106789_bib29","doi-asserted-by":"crossref","first-page":"8298","DOI":"10.1021\/acs.jmedchem.7b00089","article-title":"Exploring heme and hemoglobin binding regions of plasmodium heme detoxification protein for new antimalarial discovery","volume":"60","author":"Gupta","year":"2017","journal-title":"J. Med. Chem."},{"issue":"6557","key":"10.1016\/j.pep.2025.106789_bib30","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1126\/science.abj8754","article-title":"Accurate prediction of protein structures and interactions using a three-track neural network","volume":"373","author":"Baek","year":"2021","journal-title":"Science"}],"container-title":["Protein Expression and Purification"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592825001317?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592825001317?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T14:54:49Z","timestamp":1762354489000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046592825001317"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":30,"alternative-id":["S1046592825001317"],"URL":"https:\/\/doi.org\/10.1016\/j.pep.2025.106789","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2025.06.11.659033","asserted-by":"object"}]},"ISSN":["1046-5928"],"issn-type":[{"value":"1046-5928","type":"print"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Recombinant expression and purification of Plasmodium heme detoxification protein in E. coli: Challenges and discoveries","name":"articletitle","label":"Article Title"},{"value":"Protein Expression and Purification","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.pep.2025.106789","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"106789"}}