{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T07:26:28Z","timestamp":1773818788736,"version":"3.50.1"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100009967","name":"Xinjiang Production and Construction Corps","doi-asserted-by":"publisher","award":["2023CB008-18"],"award-info":[{"award-number":["2023CB008-18"]}],"id":[{"id":"10.13039\/501100009967","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009967","name":"Xinjiang Production and Construction Corps","doi-asserted-by":"publisher","award":["2023AB049-4"],"award-info":[{"award-number":["2023AB049-4"]}],"id":[{"id":"10.13039\/501100009967","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32060125"],"award-info":[{"award-number":["32060125"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81560614"],"award-info":[{"award-number":["81560614"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Protein Expression and Purification"],"published-print":{"date-parts":[[2026,3]]},"DOI":"10.1016\/j.pep.2025.106856","type":"journal-article","created":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T00:41:12Z","timestamp":1763772072000},"page":"106856","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Purification of antifreeze proteins from tussah silkworm (Antheraea pernyi) via polyethylene glycol precipitation coupled with aqueous two-phase extraction"],"prefix":"10.1016","volume":"239","author":[{"given":"Xinhui","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiqun","family":"Yin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingting","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taoyu","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8670-0988","authenticated-orcid":false,"given":"Yan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.pep.2025.106856_bib1","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1146\/annurev-biochem-060815-014546","article-title":"Ice-binding proteins and their function","volume":"85","author":"Bar Dolev","year":"2016","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.pep.2025.106856_bib2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/978-1-0716-3503-2_1","article-title":"Identifying ice-binding proteins in nature","volume":"2730","author":"Devries","year":"2024","journal-title":"Methods Mol. Biol."},{"issue":"3","key":"10.1016\/j.pep.2025.106856_bib3","doi-asserted-by":"crossref","DOI":"10.3390\/biom10030423","article-title":"Fish-derived antifreeze proteins and antifreeze glycoprotein exhibit a different ice-binding property with increasing concentration","volume":"10","author":"Tsuda","year":"2020","journal-title":"Biomolecules"},{"issue":"20","key":"10.1016\/j.pep.2025.106856_bib4","doi-asserted-by":"crossref","first-page":"3938","DOI":"10.1002\/jssc.201800553","article-title":"Single-step purification and characterization of antifreeze proteins from leaf and berry of a freeze-tolerant shrub seabuckthorn (Hippophae rhamnoides)","volume":"41","author":"Sharma","year":"2018","journal-title":"J. Separ. Sci."},{"issue":"77","key":"10.1016\/j.pep.2025.106856_bib5","doi-asserted-by":"crossref","first-page":"3249","DOI":"10.1098\/rsif.2012.0388","article-title":"New insights into ice growth and melting modifications by antifreeze proteins","volume":"9","author":"Bar-Dolev","year":"2012","journal-title":"J. R. Soc. Interface"},{"issue":"36","key":"10.1016\/j.pep.2025.106856_bib6","doi-asserted-by":"crossref","first-page":"33349","DOI":"10.1074\/jbc.M205575200","article-title":"A beta-helical antifreeze protein isoform with increased activity. Structural and functional insights","volume":"277","author":"Leinala","year":"2002","journal-title":"J. Biol. Chem."},{"issue":"9","key":"10.1016\/j.pep.2025.106856_bib7","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1038\/nbt0997-887","article-title":"The antifreeze potential of the spruce budworm thermal hysteresis protein","volume":"15","author":"Tyshenko","year":"1997","journal-title":"Nat. Biotechnol."},{"issue":"6793","key":"10.1016\/j.pep.2025.106856_bib8","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1038\/35018610","article-title":"\u03b2-Helix structure and ice-binding properties of a hyperactive antifreeze protein from an insect","volume":"406","author":"Graether","year":"2000","journal-title":"Nature"},{"issue":"3988","key":"10.1016\/j.pep.2025.106856_bib9","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1126\/science.172.3988.1152","article-title":"Glycoproteins as biological antifreeze agents in antarctic fishes","volume":"172","author":"Devries","year":"1971","journal-title":"Science"},{"issue":"3 Pt 1","key":"10.1016\/j.pep.2025.106856_bib10","doi-asserted-by":"crossref","first-page":"C125","DOI":"10.1152\/ajpcell.1984.247.3.C125","article-title":"Freezing of living cells: mechanisms and implications","volume":"247","author":"Mazur","year":"1984","journal-title":"Am. J. Physiol."},{"issue":"1","key":"10.1016\/j.pep.2025.106856_bib11","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1007\/BF00017731","article-title":"Cloning and characterization of a cold- and ABA-inducible Arabidopsis gene","volume":"15","author":"Kurkela","year":"1990","journal-title":"Plant Mol. Biol."},{"issue":"3\u20134","key":"10.1016\/j.pep.2025.106856_bib12","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1139\/o94-022","article-title":"A low molecular weight peptide from snow mold with epitopic homology to the winter flounder antifreeze protein","volume":"72","author":"Newsted","year":"1994","journal-title":"Biochem. Cell. Biol."},{"issue":"1","key":"10.1016\/j.pep.2025.106856_bib13","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1104\/pp.104.058628","article-title":"Antifreeze proteins modify the freezing process in planta","volume":"138","author":"Griffith","year":"2005","journal-title":"Plant Physiol."},{"key":"10.1016\/j.pep.2025.106856_bib14","series-title":"Advances in Insect Physiology","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0065-2806(08)60109-5","article-title":"Frost resistance in insects [M]\/\/","author":"Asahina","year":"1970"},{"issue":"1","key":"10.1016\/j.pep.2025.106856_bib15","first-page":"39","article-title":"A novel, intracellular antifreeze protein in an antarctic bacterium, Flavobacterium xanthum","volume":"28","author":"Kawahara","year":"2007","journal-title":"Cryo Lett."},{"issue":"1","key":"10.1016\/j.pep.2025.106856_bib16","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1089\/bio.2022.0186","article-title":"Use of antifreeze protein from Tenebrio molitor (TmAFP) in vitrification of in vitro-produced bovine embryos: an ultrastructural study","volume":"22","author":"Silva J\u00faNIOR","year":"2024","journal-title":"Biopreserv. Biobanking"},{"issue":"1","key":"10.1016\/j.pep.2025.106856_bib17","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S0378-1119(01)00635-7","article-title":"Targeted expression of a synthetic codon optimized gene, encoding the spruce budworm antifreeze protein, leads to accumulation of antifreeze activity in the apoplasts of transgenic tobacco","volume":"275","author":"Holmberg","year":"2001","journal-title":"Gene"},{"issue":"4","key":"10.1016\/j.pep.2025.106856_bib18","doi-asserted-by":"crossref","first-page":"1404","DOI":"10.1093\/jee\/tox140","article-title":"Antheraea pernyi (Lepidoptera: saturniidae) and its importance in sericulture, food consumption, and traditional Chinese medicine","volume":"110","author":"Li","year":"2017","journal-title":"J. Econ. Entomol."},{"issue":"1","key":"10.1016\/j.pep.2025.106856_bib19","first-page":"27","article-title":"Research progress on nutritional and medicinal value of tussah silkworm pupa protein","author":"Xinyue","year":"2025","journal-title":"Liaoning Silk"},{"issue":"16","key":"10.1016\/j.pep.2025.106856_bib20","doi-asserted-by":"crossref","DOI":"10.3390\/molecules27165284","article-title":"Efficient separation of proteins and polysaccharides from Dendrobium huoshanense using aqueous two-phase system with ionic liquids","volume":"27","author":"Yang","year":"2022","journal-title":"Molecules"},{"issue":"3","key":"10.1016\/j.pep.2025.106856_bib21","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1016\/S0006-291X(02)02900-5","article-title":"Purification of antifreeze proteins by adsorption to ice","volume":"300","author":"Kuiper","year":"2003","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.pep.2025.106856_bib22","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.seppur.2016.03.008","article-title":"Partitioning and purification of menadione induced NAD(P)H oxidase from phanerochaete chrysosporium in aqueous two-phase systems","volume":"163","author":"Tongul","year":"2016","journal-title":"Sep. Purif. Technol."},{"issue":"6","key":"10.1016\/j.pep.2025.106856_bib23","first-page":"600","article-title":"Preliminary study of two steps aqueous two-phase extraction combined with high performance liquid chromatography for saliva protein selective separation","volume":"32","author":"Zhao","year":"2014","journal-title":"Se Pu"},{"issue":"26","key":"10.1016\/j.pep.2025.106856_bib24","first-page":"9585","article-title":"Aqueous phase extractable protein of wheat bran and germ for the production of liquid and semi-solid foods","volume":"64","author":"Janssen","year":"2024","journal-title":"Crit. Rev. Food Sci. Nutr."},{"issue":"2","key":"10.1016\/j.pep.2025.106856_bib25","first-page":"565","article-title":"Optimization of complexation extraction and back-extraction process for oxymatrine in supernatant of Sophora flavescens","volume":"47","author":"Lei","year":"2025","journal-title":"Chin. Tradit. Pat. Med."},{"key":"10.1016\/j.pep.2025.106856_bib26","doi-asserted-by":"crossref","DOI":"10.1016\/j.cryobiol.2023.104584","article-title":"Droplet freezing assays using a nanoliter osmometer","volume":"113","author":"Lee","year":"2023","journal-title":"Cryobiology"},{"issue":"5259","key":"10.1016\/j.pep.2025.106856_bib27","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.pep.2025.106856_bib28","series-title":"Extraction of Polysaccharides from Malus hupehensis by Aqueous Two-phase System and its Physiological Activity [D]","author":"Pengcheng","year":"2022"},{"issue":"3","key":"10.1016\/j.pep.2025.106856_bib29","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/j.cryobiol.2005.07.007","article-title":"The mechanism by which fish antifreeze proteins cause thermal hysteresis","volume":"51","author":"Kristiansen","year":"2005","journal-title":"Cryobiology"},{"issue":"16","key":"10.1016\/j.pep.2025.106856_bib30","doi-asserted-by":"crossref","first-page":"3285","DOI":"10.1111\/j.1432-1033.2004.04256.x","article-title":"Cold survival in freeze-intolerant insects: the structure and function of beta-helical antifreeze proteins","volume":"271","author":"Graether","year":"2004","journal-title":"Eur. J. Biochem."},{"issue":"4","key":"10.1016\/j.pep.2025.106856_bib31","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1007\/s00239-005-0256-3","article-title":"Evolution of hyperactive, repetitive antifreeze proteins in beetles","volume":"64","author":"Graham","year":"2007","journal-title":"J. Mol. Evol."},{"issue":"4","key":"10.1016\/j.pep.2025.106856_bib32","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.jinsphys.2003.12.003","article-title":"Antifreeze proteins in Alaskan insects and spiders","volume":"50","author":"Duman","year":"2004","journal-title":"J. Insect Physiol."},{"issue":"9","key":"10.1016\/j.pep.2025.106856_bib33","doi-asserted-by":"crossref","first-page":"4571","DOI":"10.1021\/acs.jcim.1c00505","article-title":"Quantifying the separation of positive and negative areas in electrostatic potential for predicting feasibility of ammonium sulfate for protein crystallization","volume":"61","author":"Guo","year":"2021","journal-title":"J. Chem. Inf. Model."},{"issue":"6","key":"10.1016\/j.pep.2025.106856_bib34","doi-asserted-by":"crossref","DOI":"10.1063\/5.0212953","article-title":"A random sequential adsorption model for the irreversible binding of Tenebrio molitor antifreeze protein to ice crystals","volume":"14","author":"Guo","year":"2024","journal-title":"AIP Adv."},{"issue":"Pt 4","key":"10.1016\/j.pep.2025.106856_bib35","article-title":"The low-entropy hydration shell mediated ice-binding mechanism of antifreeze proteins","volume":"277","author":"Guo","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"issue":"17","key":"10.1016\/j.pep.2025.106856_bib36","doi-asserted-by":"crossref","first-page":"5661","DOI":"10.1128\/JB.186.17.5661-5671.2004","article-title":"Cloning and expression of afpA, a gene encoding an antifreeze protein from the arctic plant growth-promoting rhizobacterium Pseudomonas putida GR12-2","volume":"186","author":"Muryoi","year":"2004","journal-title":"J. Bacteriol."},{"issue":"4","key":"10.1016\/j.pep.2025.106856_bib37","article-title":"Discovery of antifreeze proteins in the leaves of the ice margin plant eupatorium in the cold region of tianshan Mountains","author":"Ji","year":"2001","journal-title":"Glacial Permafrost"},{"issue":"1","key":"10.1016\/j.pep.2025.106856_bib38","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-018-29312-x","article-title":"Falling water ice affinity purification of ice-binding proteins","volume":"8","author":"Adar","year":"2018","journal-title":"Sci. Rep."},{"issue":"5","key":"10.1016\/j.pep.2025.106856_bib39","doi-asserted-by":"crossref","DOI":"10.3390\/biom9050180","article-title":"Laboratory-scale isolation of insect antifreeze protein for cryobiology","volume":"9","author":"Tomalty","year":"2019","journal-title":"Biomolecules"},{"issue":"3","key":"10.1016\/j.pep.2025.106856_bib40","doi-asserted-by":"crossref","first-page":"153","DOI":"10.4265\/bio.21.153","article-title":"Antifreeze activity of xylomannan from the mycelium and fruit body of Flammulina velutipes","volume":"21","author":"Kawahara","year":"2016","journal-title":"Biocontrol Sci."},{"issue":"Pt 10","key":"10.1016\/j.pep.2025.106856_bib41","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1107\/S2053230X24008197","article-title":"Protein expression, purification, crystallization and crystallographic studies of BPSL0741 from burkholderia pseudomallei","volume":"80","author":"Fadhar","year":"2024","journal-title":"Acta Crystallographica Section F Structural Biol. Commun."},{"issue":"3","key":"10.1016\/j.pep.2025.106856_bib42","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/0167-4781(93)90062-I","article-title":"Isolation and characterization of an antifreeze protein precursor from transgenic drosophila: evidence for partial processing","volume":"1171","author":"Peters","year":"1993","journal-title":"Biochim. Biophys. Acta"},{"issue":"1","key":"10.1016\/j.pep.2025.106856_bib43","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1186\/s13048-025-01793-1","article-title":"The mechanism and protective strategies of follicle injury after ovarian tissue cryopreservation and thawed transplantation: a review","volume":"18","author":"Chu","year":"2025","journal-title":"J. Ovarian Res."},{"issue":"Pt 1","key":"10.1016\/j.pep.2025.106856_bib44","article-title":"Design and applications of antifreeze polysaccharide-based hydrogels for cryoprotection and biotechnological advancements: a review","volume":"310","author":"Wang","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"issue":"18","key":"10.1016\/j.pep.2025.106856_bib45","doi-asserted-by":"crossref","DOI":"10.3390\/ijms251810110","article-title":"Molecular mechanisms underlying freezing tolerance in plants: implications for cryopreservation","volume":"25","author":"Bia\u0142osk\u00f3RSKA","year":"2024","journal-title":"Int. J. Mol. Sci."},{"issue":"6","key":"10.1016\/j.pep.2025.106856_bib46","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.1007\/s10695-018-0538-5","article-title":"Protective role of antifreeze proteins on sterlet (Acipenser ruthenus) sperm during cryopreservation","volume":"44","author":"Xin","year":"2018","journal-title":"Fish Physiol. Biochem."},{"issue":"3","key":"10.1016\/j.pep.2025.106856_bib47","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0318459","article-title":"Antifreeze proteins produced by antarctic yeast from the genus glaciozyma as cryoprotectants in food storage","volume":"20","author":"Majewska","year":"2025","journal-title":"PLoS One"},{"key":"10.1016\/j.pep.2025.106856_bib48","article-title":"Effects of ice-binding protein from leucosporidium sp. AY30 (LeIBP) on physicochemical quality, myofibrillar protein and ice crystal morphology of largemouth bass during air freezing","volume":"220","author":"Ren","year":"2025","journal-title":"Food research international (Ottawa, Ont)"},{"issue":"2","key":"10.1016\/j.pep.2025.106856_bib49","doi-asserted-by":"crossref","DOI":"10.3390\/biom14020174","article-title":"Novel apoplastic antifreeze proteins of Deschampsia Antarctica as enhancer of common cell freezing media for cryobanking of genetic resources, a preliminary study","volume":"14","author":"Short","year":"2024","journal-title":"Biomolecules"}],"container-title":["Protein Expression and Purification"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592825001986?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592825001986?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T06:19:55Z","timestamp":1773814795000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046592825001986"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":49,"alternative-id":["S1046592825001986"],"URL":"https:\/\/doi.org\/10.1016\/j.pep.2025.106856","relation":{},"ISSN":["1046-5928"],"issn-type":[{"value":"1046-5928","type":"print"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Purification of antifreeze proteins from tussah silkworm (Antheraea pernyi) via polyethylene glycol precipitation coupled with aqueous two-phase extraction","name":"articletitle","label":"Article Title"},{"value":"Protein Expression and Purification","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.pep.2025.106856","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"106856"}}