{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T07:17:51Z","timestamp":1778570271462,"version":"3.51.4"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100008725","name":"National Agency for Research and Innovation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Protein Expression and Purification"],"published-print":{"date-parts":[[2026,7]]},"DOI":"10.1016\/j.pep.2026.106902","type":"journal-article","created":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T16:39:09Z","timestamp":1771519149000},"page":"106902","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Exploring elastin-like polypeptide tags and mini-intein for recombinant protein purification in Leishmania tarentolae"],"prefix":"10.1016","volume":"241","author":[{"given":"Luc\u00eda","family":"Bassetti","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Crispo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariela","family":"Bollati-Fogol\u00edn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"Pantano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo A.","family":"Comini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1143-6689","authenticated-orcid":false,"given":"Cecilia","family":"Abreu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.pep.2026.106902_bib1","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2021.654289","article-title":"Novel vaccine technologies in veterinary medicine: a herald to human medicine vaccines","volume":"8","author":"Aida","year":"2021","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.pep.2026.106902_bib2","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/j.fsi.2022.09.011","article-title":"Bioactive immunostimulants as health-promoting feed additives in aquaculture: a review","volume":"130","author":"Vijayaram","year":"2022","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.pep.2026.106902_bib3","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2023.1248879","article-title":"GnRH-immunocastration: an alternative method for male animal surgical castration","volume":"10","author":"Wang","year":"2023","journal-title":"Front. Vet. Sci."},{"issue":"2","key":"10.1016\/j.pep.2026.106902_bib4","doi-asserted-by":"crossref","DOI":"10.1590\/1984-3143-ar2023-0092","article-title":"Use of new recombinant proteins for ovarian stimulation in ruminants","volume":"20","author":"Baruselli","year":"2023","journal-title":"Anim. Reprod."},{"issue":"5","key":"10.1016\/j.pep.2026.106902_bib5","doi-asserted-by":"crossref","first-page":"590","DOI":"10.14202\/vetworld.2015.590-598","article-title":"Role of parasitic vaccines in integrated control of parasitic diseases in livestock","volume":"8","author":"Sharma","year":"2015","journal-title":"Vet. World"},{"issue":"1","key":"10.1016\/j.pep.2026.106902_bib6","doi-asserted-by":"crossref","DOI":"10.1186\/s12934-017-0654-4","article-title":"Trends in recombinant protein use in animal production","volume":"16","author":"Gifre","year":"2017","journal-title":"Microb. Cell Fact."},{"issue":"7","key":"10.1016\/j.pep.2026.106902_bib7","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0007424","article-title":"Leishmania tarentolae: taxonomic classification and its application as a promising biotechnological expression host","volume":"13","author":"Klatt","year":"2019","journal-title":"PLoS Neglected Trop. Dis."},{"issue":"3","key":"10.1016\/j.pep.2026.106902_bib8","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1080\/07388551.2019.1566206","article-title":"Potential application of Leishmania tarentolae as an alternative platform for antibody expression","volume":"39","author":"Lai","year":"2019","journal-title":"Crit. Rev. Biotechnol."},{"key":"10.1016\/j.pep.2026.106902_bib9","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.procbio.2019.08.019","article-title":"Application of the LEXSY Leishmania tarentolae system as a recombinant protein expression platform: a review","volume":"87","author":"de Oliveira","year":"2019","journal-title":"Process Biochem."},{"issue":"4","key":"10.1016\/j.pep.2026.106902_bib10","doi-asserted-by":"crossref","DOI":"10.1111\/1751-7915.14444","article-title":"Expression and functional characterization of chimeric recombinant bovine follicle-stimulating hormone produced in Leishmania tarentolae","volume":"17","author":"Abreu","year":"2024","journal-title":"Microb. Biotechnol."},{"key":"10.1016\/j.pep.2026.106902_bib11","series-title":"En Comprehensive Biotechnology, Second Edition","first-page":"811","article-title":"The proportion of downstream costs in fermentative production processes","author":"Straathof","year":"2011"},{"issue":"4","key":"10.1016\/j.pep.2026.106902_bib12","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1021\/bp00016a012","article-title":"Production and purification of a recombinant elastomeric polypeptide, G\u2010(VPGVG)19\u2010VPGV, from Escherichia coli","volume":"8","author":"Mcpherson","year":"1992","journal-title":"Biotechnol. Prog."},{"issue":"4","key":"10.1016\/j.pep.2026.106902_bib13","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1021\/bp010049o","article-title":"Protein purification by fusion with an environmentally responsive elastin-like polypeptide: effect of polypeptide length on the purification of thioredoxin","volume":"17","author":"Meyer","year":"2001","journal-title":"Biotechnol. Prog."},{"issue":"5","key":"10.1016\/j.pep.2026.106902_bib14","doi-asserted-by":"crossref","first-page":"1514","DOI":"10.1021\/bm400167h","article-title":"Predicting transition temperatures of elastin-like polypeptide fusion proteins","volume":"14","author":"Christensen","year":"2013","journal-title":"Biomacromolecules"},{"issue":"2","key":"10.1016\/j.pep.2026.106902_bib15","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.pep.2009.03.009","article-title":"Optimization of ELP-intein mediated protein purification by salt substitution","volume":"66","author":"Fong","year":"2009","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.pep.2026.106902_bib16","doi-asserted-by":"crossref","DOI":"10.1038\/15100","article-title":"Purification of recombinant proteins by fusion with thermally-responsive polypeptides","volume":"17","author":"Meyer","year":"1999","journal-title":"Nat. Biotechnol."},{"issue":"4","key":"10.1016\/j.pep.2026.106902_bib17","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1007\/s002530050942","article-title":"Expression of a synthetic protein-based polymer (elastomer) gene in Aspergillus nidulans","volume":"47","author":"Herzog","year":"1997","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.pep.2026.106902_bib18","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.pep.2018.09.006","article-title":"Biotechnological applications of elastin-like polypeptides and the inverse transition cycle in the pharmaceutical industry","volume":"153","author":"Fletcher","year":"2019","journal-title":"Protein Expr. Purif."},{"issue":"1","key":"10.1016\/j.pep.2026.106902_bib19","doi-asserted-by":"crossref","DOI":"10.3390\/microorganisms6010019","article-title":"Inteins: localized distribution, gene regulation, and protein engineering for biological applications","volume":"6","author":"Pavankumar","year":"2018","journal-title":"Microorganisms"},{"issue":"2","key":"10.1016\/j.pep.2026.106902_bib20","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.pep.2013.05.007","article-title":"Expression and purification of functional human glycogen synthase-1 (hGYS1) in insect cells","volume":"90","author":"Khanna","year":"2013","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.pep.2026.106902_bib21","author":"Liu"},{"key":"10.1016\/j.pep.2026.106902_bib22","doi-asserted-by":"crossref","DOI":"10.1038\/12879","article-title":"A genetic system yields self-cleaving inteins for bioseparations","volume":"17","author":"Wood","year":"1999","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.pep.2026.106902_bib23","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.theriogenology.2020.02.001","article-title":"Superstimulation of ovarian follicles in cattle: Gonadotropin treatment protocols and FSH profiles","volume":"150","author":"B\u00f3","year":"2020","journal-title":"Theriogenology"},{"issue":"3","key":"10.1016\/j.pep.2026.106902_bib24","first-page":"264","article-title":"Development of recombinant follicle-stimulating hormone for the superovulation of cattle: a review","volume":"12","author":"Zhang","year":"2025","journal-title":"Vet. Sci."},{"issue":"9","key":"10.1016\/j.pep.2026.106902_bib25","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1038\/nmeth787","article-title":"Simple bioseparations using self-cleaving elastin-like polypeptide tags","volume":"2","author":"Banki","year":"2005","journal-title":"Nat. Methods"},{"key":"10.1016\/j.pep.2026.106902_bib26","doi-asserted-by":"crossref","DOI":"10.1186\/1475-2859-11-97","article-title":"Secretory signal peptide modification for optimized antibody-fragment expression-secretion in Leishmania tarentolae","volume":"11","author":"Klatt","year":"2012","journal-title":"Microb. Cell Fact."},{"issue":"1","key":"10.1016\/j.pep.2026.106902_bib27","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-017-09926-3","article-title":"The EAL-domain protein FcsR regulates flagella, chemotaxis and type III secretion system in Pseudomonas aeruginosa by a phosphodiesterase independent mechanism","volume":"7","author":"Rossello","year":"2017","journal-title":"Sci. Rep."},{"issue":"3","key":"10.1016\/j.pep.2026.106902_bib28","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1021\/bm034215n","article-title":"Quantification of the effects of chain length and concentration on the thermal behavior of elastin-like polypeptides","volume":"5","author":"Meyer","year":"2004","journal-title":"Biomacromolecules"},{"issue":"1","key":"10.1016\/j.pep.2026.106902_bib29","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.ab.2006.09.020","article-title":"Purification of recombinant proteins from Escherichia coli at low expression levels by inverse transition cycling","volume":"360","author":"Christensen","year":"2007","journal-title":"Anal. Biochem."},{"issue":"1","key":"10.1016\/j.pep.2026.106902_bib30","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1038\/nrm2820","article-title":"Sensors and regulators of intracellular pH","volume":"11","author":"Casey","year":"2010","journal-title":"Nat. Rev. Mol. Cell Biol."},{"issue":"2","key":"10.1016\/j.pep.2026.106902_bib31","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1093\/gbe\/evz017","article-title":"The tritryps comparative repeatome: insights on repetitive element evolution in trypanosomatid pathogens","volume":"11","author":"Pita","year":"2019","journal-title":"Genome Biol. Evol."},{"key":"10.1016\/j.pep.2026.106902_bib32","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2023.1102462","article-title":"Life in plastic, it\u2019s fantastic! How Leishmania exploit genome instability to shape gene expression","volume":"13","author":"Black","year":"2023","journal-title":"Front. Cell. Infect. Microbiol."},{"issue":"5","key":"10.1016\/j.pep.2026.106902_bib33","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.1001868","article-title":"Genome-wide stochastic adaptive DNA amplification at direct and inverted DNA repeats in the parasite Leishmania","volume":"12","author":"Ubeda","year":"2014","journal-title":"PLoS Biol."},{"key":"10.1016\/j.pep.2026.106902_bib34","doi-asserted-by":"crossref","DOI":"10.1002\/0471140864.ps0611s61","article-title":"Elastin-like polypeptides as a purification tag for recombinant proteins","volume":"61","author":"Hassouneh","year":"2010","journal-title":"Curr. Protoc. in Protein Sci."},{"key":"10.1016\/j.pep.2026.106902_bib35","doi-asserted-by":"crossref","DOI":"10.1186\/1475-2859-4-32","article-title":"Inteins and affinity resin substitutes for protein purification and scale up","volume":"4","author":"Banki","year":"2005","journal-title":"Microb. Cell Fact."}],"container-title":["Protein Expression and Purification"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592826000252?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592826000252?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T07:01:13Z","timestamp":1778569273000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046592826000252"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":35,"alternative-id":["S1046592826000252"],"URL":"https:\/\/doi.org\/10.1016\/j.pep.2026.106902","relation":{},"ISSN":["1046-5928"],"issn-type":[{"value":"1046-5928","type":"print"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Exploring elastin-like polypeptide tags and mini-intein for recombinant protein purification in Leishmania tarentolae","name":"articletitle","label":"Article Title"},{"value":"Protein Expression and Purification","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.pep.2026.106902","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"106902"}}