{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T12:54:28Z","timestamp":1791550468081,"version":"4.3.4"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100011785","name":"Science and Technology Department of Guangxi Zhuang Autonomous Region","doi-asserted-by":"publisher","award":["AA24206010"],"award-info":[{"award-number":["AA24206010"]}],"id":[{"id":"10.13039\/501100011785","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011827","name":"Guangxi Medical University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011827","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82260048"],"award-info":[{"award-number":["82260048"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Protein Expression and Purification"],"published-print":{"date-parts":[[2027,2]]},"DOI":"10.1016\/j.pep.2026.107018","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T15:21:58Z","timestamp":1790608918000},"page":"107018","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Structural insights into the catalytic mechanism of SsChiA from Sporisorium scitamineum"],"prefix":"10.1016","volume":"244","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-3384-8079","authenticated-orcid":false,"given":"Jianing","family":"Wei","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8755-3457","authenticated-orcid":false,"given":"Chao","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjie","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenhua","family":"Ming","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beibei","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.pep.2026.107018_bib1","doi-asserted-by":"crossref","first-page":"2623","DOI":"10.1007\/s10722-022-01430-6","article-title":"A short review on sugarcane: its domestication, molecular manipulations and future perspectives","volume":"69","author":"Dinesh Babu","year":"2022","journal-title":"Genet. Resour. Crop Evol."},{"key":"10.1016\/j.pep.2026.107018_bib2","doi-asserted-by":"crossref","DOI":"10.1126\/science.adx1616","article-title":"Multiscale pangenome graphs empower the genomic dissection of mixed-ploidy sugarcane species","volume":"391","author":"Huang","year":"2026","journal-title":"Science"},{"key":"10.1016\/j.pep.2026.107018_bib3","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1186\/1471-2164-15-996","article-title":"Genome sequencing of Sporisorium scitamineum provides insights into the pathogenic mechanisms of sugarcane smut","volume":"15","author":"Que","year":"2014","journal-title":"BMC Genom."},{"key":"10.1016\/j.pep.2026.107018_bib4","doi-asserted-by":"crossref","first-page":"1905","DOI":"10.1094\/PHYTO-05-21-0221-RVW","article-title":"Sugarcane smut, caused by Sporisorium scitamineum, a major disease of sugarcane: a contemporary review","volume":"111","author":"Bhuiyan","year":"2021","journal-title":"Phytopathology"},{"key":"10.1016\/j.pep.2026.107018_bib5","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.3390\/jof7121095","article-title":"Sugarcane smut: current knowledge and the way forward for management","volume":"7","author":"Rajput","year":"2021","journal-title":"J. Fungi"},{"key":"10.1016\/j.pep.2026.107018_bib6","doi-asserted-by":"crossref","DOI":"10.1111\/mpp.70191","article-title":"Pathogenesis, host resistance and integrated management of sugarcane smut caused by Sporisorium scitamineum: a comprehensive review","volume":"26","author":"Zeng","year":"2025","journal-title":"Mol. Plant Pathol."},{"key":"10.1016\/j.pep.2026.107018_bib7","doi-asserted-by":"crossref","first-page":"2303","DOI":"10.1094\/PHYTO-03-21-0114-R","article-title":"Genetic dissection of T-DNA insertional mutants reveals uncoupling of dikaryotic filamentation and virulence in sugarcane smut fungus","volume":"111","author":"Lu","year":"2021","journal-title":"Phytopathology"},{"key":"10.1016\/j.pep.2026.107018_bib8","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0129318","article-title":"Complete genome sequence of Sporisorium scitamineum and biotrophic interaction transcriptome with sugarcane","volume":"10","author":"Taniguti","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.pep.2026.107018_bib9","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1093\/gbe\/evw026","article-title":"A tale of genome compartmentalization: the evolution of virulence clusters in smut fungi","volume":"8","author":"Dutheil","year":"2016","journal-title":"Genome Biol. Evol."},{"key":"10.1016\/j.pep.2026.107018_bib10","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1007\/s13205-021-02893-7","article-title":"Comparative expression analysis of potential pathogenicity-associated genes of high- and low-virulent Sporisorium scitamineum isolates during interaction with sugarcane","volume":"11","author":"Nalayeni","year":"2021","journal-title":"3 Biotech"},{"key":"10.1016\/j.pep.2026.107018_bib11","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.fgb.2015.12.004","article-title":"\u03b2-glucan: crucial component of the fungal cell wall and elusive MAMP in plants","volume":"90","author":"Fesel","year":"2016","journal-title":"Fungal Genet. Biol."},{"key":"10.1016\/j.pep.2026.107018_bib12","doi-asserted-by":"crossref","first-page":"1786","DOI":"10.1016\/j.biotechadv.2013.09.012","article-title":"From bacteria to human: a journey into the world of chitinases","volume":"31","author":"Adrangi","year":"2013","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.pep.2026.107018_bib13","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1007\/978-981-13-7318-3_4","article-title":"Immune responses of mammals and plants to chitin-containing pathogens","volume":"1142","author":"Jiang","year":"2019","journal-title":"Adv. Exp. Med. Biol."},{"key":"10.1016\/j.pep.2026.107018_bib14","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1093\/plcell\/koab011","article-title":"Effectors with chitinase activity (EWCAs), a family of conserved, secreted fungal chitinases that suppress chitin-triggered immunity","volume":"33","author":"Mart\u00ednez-Cruz","year":"2021","journal-title":"Plant Cell"},{"key":"10.1016\/j.pep.2026.107018_bib15","doi-asserted-by":"crossref","first-page":"2000","DOI":"10.1093\/plcell\/koae027","article-title":"Rice false smut virulence protein subverts host chitin perception and signaling at lemma and palea for floral infection","volume":"36","author":"Li","year":"2024","journal-title":"Plant Cell"},{"key":"10.1016\/j.pep.2026.107018_bib16","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1042\/bj2930781","article-title":"New families in the classification of glycosyl hydrolases based on amino acid sequence similarities","volume":"293","author":"Henrissat","year":"1993","journal-title":"Biochem. J."},{"key":"10.1016\/j.pep.2026.107018_bib17","first-page":"208","article-title":"Evolution of family 18 glycoside hydrolases: diversity, domain structures and phylogenetic relationships","volume":"16","author":"Karlsson","year":"2009","journal-title":"J. Mol. Microbiol. Biotechnol."},{"key":"10.1016\/j.pep.2026.107018_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.biotechadv.2020.107553","article-title":"Glycoside hydrolase family 18 chitinases: the known and the unknown","volume":"43","author":"Chen","year":"2020","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.pep.2026.107018_bib19","article-title":"The modular evolution of chitinases is governed by coevolution of auxiliary and catalytic domains","author":"Pulsford","year":"2026","journal-title":"bioRxiv"},{"key":"10.1016\/j.pep.2026.107018_bib20","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1016\/S0969-2126(01)00220-9","article-title":"Structures and mechanisms of glycosyl hydrolases","volume":"3","author":"Davies","year":"1995","journal-title":"Structure"},{"key":"10.1016\/j.pep.2026.107018_bib21","doi-asserted-by":"crossref","first-page":"296","DOI":"10.7164\/antibiotics.40.296","article-title":"Search for microbial insect growth regulators. II. Allosamidin, a novel insect chitinase inhibitor","volume":"40","author":"Sakuda","year":"1987","journal-title":"J. Antibiot."},{"key":"10.1016\/j.pep.2026.107018_bib22","doi-asserted-by":"crossref","first-page":"609","DOI":"10.7164\/antibiotics.53.609","article-title":"Argifin, a new chitinase inhibitor, produced by Gliocladium sp. FTD-0668. II. Isolation, physico-chemical properties, and structure elucidation","volume":"53","author":"Arai","year":"2000","journal-title":"J. Antibiot."},{"key":"10.1016\/j.pep.2026.107018_bib23","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","article-title":"Accurate structure prediction of biomolecular interactions with AlphaFold 3","volume":"630","author":"Abramson","year":"2024","journal-title":"Nature"},{"key":"10.1016\/j.pep.2026.107018_bib24","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1107\/S090744490705024X","article-title":"Iterative model building, structure refinement and density modification with the PHENIX AutoBuild wizard","volume":"64","author":"Terwilliger","year":"2008","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.pep.2026.107018_bib25","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1107\/S0907444910007493","article-title":"Features and development of Coot","volume":"66","author":"Emsley","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.pep.2026.107018_bib26","doi-asserted-by":"crossref","first-page":"1948","DOI":"10.1107\/S0907444902016657","article-title":"PHENIX: building new software for automated crystallographic structure determination","volume":"58","author":"Adams","year":"2002","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.pep.2026.107018_bib27","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1002\/jcc.21334","article-title":"AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading","volume":"31","author":"Trott","year":"2010","journal-title":"J. Comput. Chem."},{"key":"10.1016\/j.pep.2026.107018_bib28","doi-asserted-by":"crossref","first-page":"2778","DOI":"10.1021\/ci200227u","article-title":"LigPlot+: multiple ligand-protein interaction diagrams for drug discovery","volume":"51","author":"Laskowski","year":"2011","journal-title":"J. Chem. Inf. Model."},{"key":"10.1016\/j.pep.2026.107018_bib29","doi-asserted-by":"crossref","first-page":"18567","DOI":"10.1016\/S0021-9258(17)46665-8","article-title":"Identification of glutamic acid 204 and aspartic acid 200 in chitinase A1 of Bacillus circulans WL-12 as essential residues for chitinase activity","volume":"268","author":"Watanabe","year":"1993","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.pep.2026.107018_bib30","doi-asserted-by":"crossref","first-page":"8979","DOI":"10.1073\/pnas.151103798","article-title":"Structural insights into the catalytic mechanism of a family 18 exo-chitinase","volume":"98","author":"van Aalten","year":"2001","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.pep.2026.107018_bib31","doi-asserted-by":"crossref","first-page":"11338","DOI":"10.1021\/bi010505h","article-title":"High resolution structural analyses of mutant chitinase A complexes with substrates provide new insight into the mechanism of catalysis","volume":"40","author":"Papanikolau","year":"2001","journal-title":"Biochemistry"},{"key":"10.1016\/j.pep.2026.107018_bib32","doi-asserted-by":"crossref","first-page":"19258","DOI":"10.1002\/chem.201803905","article-title":"Oxazoline or oxazolinium ion? The protonation state and conformation of the reaction intermediate of chitinase enzymes revisited","volume":"24","author":"Coines","year":"2018","journal-title":"Chem. Eur J."},{"key":"10.1016\/j.pep.2026.107018_bib33","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1006\/jmbi.1996.0510","article-title":"The 1.8 \u00c5 resolution structure of hevamine, a plant chitinase\/lysozyme, and analysis of the conserved sequence and structure motifs of glycosyl hydrolase family 18","volume":"262","author":"Terwisscha van Scheltinga","year":"1996","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.pep.2026.107018_bib34","doi-asserted-by":"crossref","first-page":"3975","DOI":"10.1038\/ncomms4975","article-title":"Two-way traffic of glycoside hydrolase family 18 processive chitinases on crystalline chitin","volume":"5","author":"Igarashi","year":"2014","journal-title":"Nat. Commun."},{"key":"10.1016\/j.pep.2026.107018_bib35","doi-asserted-by":"crossref","first-page":"2080","DOI":"10.1074\/jbc.M116.755330","article-title":"Structure, catalysis, and inhibition of OfChi-h, the Lepidoptera-exclusive insect chitinase","volume":"292","author":"Liu","year":"2017","journal-title":"J. Biol. Chem."}],"container-title":["Protein Expression and Purification"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592826001415?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1046592826001415?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T12:28:14Z","timestamp":1791548894000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1046592826001415"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2027,2]]},"references-count":35,"alternative-id":["S1046592826001415"],"URL":"https:\/\/doi.org\/10.1016\/j.pep.2026.107018","relation":{},"ISSN":["1046-5928"],"issn-type":[{"value":"1046-5928","type":"print"}],"subject":[],"published":{"date-parts":[[2027,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Structural insights into the catalytic mechanism of SsChiA from Sporisorium scitamineum","name":"articletitle","label":"Article Title"},{"value":"Protein Expression and Purification","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.pep.2026.107018","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"107018"}}