{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T15:39:59Z","timestamp":1778686799534,"version":"3.51.4"},"reference-count":51,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T00:00:00Z","timestamp":1349049600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,8,27]],"date-time":"2013-08-27T00:00:00Z","timestamp":1377561600000},"content-version":"vor","delay-in-days":330,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"name":"CNPq (Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico)"},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"name":"UCB"},{"DOI":"10.13039\/501100005668","name":"FAPDF","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005668","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Peptides"],"published-print":{"date-parts":[[2012,10]]},"DOI":"10.1016\/j.peptides.2012.07.021","type":"journal-article","created":{"date-parts":[[2012,8,3]],"date-time":"2012-08-03T01:01:49Z","timestamp":1343955709000},"page":"301-308","source":"Crossref","is-referenced-by-count":40,"title":["Predicting antimicrobial peptides from eukaryotic genomes: In silico strategies to develop antibiotics"],"prefix":"10.1016","volume":"37","author":[{"given":"Andr\u00e9 C.","family":"Amaral","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osmar N.","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nath\u00e1lia C.C.R.","family":"Mundim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria J.A.","family":"de Carvalho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ludovico","family":"Migliolo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose R.S.A.","family":"Leite","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maura V.","family":"Prates","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anam\u00e9lia L.","family":"Bocca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oct\u00e1vio L.","family":"Franco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria S.S.","family":"Felipe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"11","key":"10.1016\/j.peptides.2012.07.021_bib0005","doi-asserted-by":"crossref","first-page":"2411","DOI":"10.1016\/j.bbamem.2009.08.021","article-title":"Structure\u2013function study of cathelicidin-derived bovine antimicrobial peptide BMAP-28: design of its cell-selective analogs by amino acid substitutions in the heptad repeat sequences","volume":"1788","author":"Ahmad","year":"2009","journal-title":"BBA Biomembranes"},{"key":"10.1016\/j.peptides.2012.07.021_bib0010","doi-asserted-by":"crossref","first-page":"224L 233","DOI":"10.1016\/S0925-4439(02)00085-6","article-title":"From natural products to clinically useful antifungals","volume":"1587","author":"Barrett","year":"2002","journal-title":"Biochim Biophys Acta"},{"key":"10.1016\/j.peptides.2012.07.021_bib0015","doi-asserted-by":"crossref","first-page":"586L 589","DOI":"10.1093\/nar\/gkh032","article-title":"Antimic: a database of antimicrobial sequences","volume":"32","author":"Brahmachary","year":"2004","journal-title":"Nucleic Acids Res"},{"issue":"2","key":"10.1016\/j.peptides.2012.07.021_bib0020","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1111\/j.1365-2958.2006.05525.x","article-title":"Genome based discovery, structure prediction and functional analysis of cyclic lipopeptide antibiotics in Pseudomonas species","volume":"62","author":"Bruijn","year":"2007","journal-title":"Mol Microbiol"},{"issue":"5","key":"10.1016\/j.peptides.2012.07.021_bib0025","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1093\/jac\/dkl056","article-title":"Activity of novel non-amphipathic cationic antimicrobial peptides against Candida species","volume":"57","author":"Burrows","year":"2006","journal-title":"J Antimicrob Chemother"},{"issue":"1","key":"10.1016\/j.peptides.2012.07.021_bib0030","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/s00726-010-0622-3","article-title":"Antimicrobial peptides from Phyllomedusa frogs: from biomolecular diversity to potential nanotechnologic medical applications","volume":"40","author":"Calderon","year":"2010","journal-title":"Amino Acids"},{"key":"10.1016\/j.peptides.2012.07.021_bib0035","doi-asserted-by":"crossref","first-page":"2404","DOI":"10.1016\/j.pep.2004.12.007","article-title":"Expression and purification of antimicrobial peptide adenoregulin with C-amidated terminus in Escherichia coli","volume":"40","author":"Cao","year":"2005","journal-title":"Protein Expr Purif"},{"issue":"2","key":"10.1016\/j.peptides.2012.07.021_bib0040","first-page":"130","article-title":"Antifungal agents: mode of action in yeast cells","volume":"19","author":"Carrillo-Mu\u00f1oz","year":"2006","journal-title":"Rev Esp Quimioter"},{"key":"10.1016\/j.peptides.2012.07.021_bib0045","doi-asserted-by":"crossref","first-page":"3009","DOI":"10.1021\/bi00009a032","article-title":"NMR solution structure of the antifungal protein from Aspergillus giganteus: evidence for cysteine pairing isomerism","volume":"34","author":"Campos-Olivas","year":"1995","journal-title":"Biochemistry"},{"issue":"4","key":"10.1016\/j.peptides.2012.07.021_bib0050","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1007\/s00249-011-0677-4","article-title":"The effect of membrane curvature on the conformation of antimicrobial peptides: implications for binding and the mechanism of action","volume":"40","author":"Chen","year":"2011","journal-title":"Eur Biophys J"},{"key":"10.1016\/j.peptides.2012.07.021_bib0055","series-title":"Reference method for broth dilution antifungal susceptibility testing of yeasts, approved standard","author":"CLSI","year":"2008"},{"key":"10.1016\/j.peptides.2012.07.021_bib0060","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1038\/nrmicro1537","article-title":"Advances in combating fungal diseases: vaccines on the threshold","volume":"5","author":"Cutler","year":"2007","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.peptides.2012.07.021_bib0065","doi-asserted-by":"crossref","first-page":"19424","DOI":"10.1016\/S0021-9258(19)77650-9","article-title":"A family of bacteria-regulated, cecropin D-like peptides from Manduca sexta","volume":"263","author":"Dickinson","year":"1988","journal-title":"J Biol Chem"},{"issue":"12","key":"10.1016\/j.peptides.2012.07.021_bib0070","doi-asserted-by":"crossref","first-page":"7495","DOI":"10.4049\/jimmunol.172.12.7495","article-title":"Coupling in silico and in vitro analysis of peptide-MHC binding: a bioinformatic approach enabling prediction of superbinding peptides and anchorless epitopes","volume":"172","author":"Doytchinova","year":"2004","journal-title":"J Immunol"},{"issue":"26","key":"10.1016\/j.peptides.2012.07.021_bib0075","doi-asserted-by":"crossref","first-page":"24706","DOI":"10.1074\/jbc.M500625200","article-title":"Transcriptional profiles of the human pathogenic fungus Paracoccidioides brasiliensis in mycelium and yeast cells","volume":"280","author":"Felipe","year":"2005","journal-title":"J Biol Chem"},{"issue":"9","key":"10.1016\/j.peptides.2012.07.021_bib0080","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/S1359-6446(03)02684-9","article-title":"Superpeptide to treat Candida albicans","volume":"8","author":"Foubister","year":"2003","journal-title":"Drug Discov Today"},{"key":"10.1016\/j.peptides.2012.07.021_bib0085","doi-asserted-by":"crossref","first-page":"2485L 2492","DOI":"10.1099\/mic.0.048736-0","article-title":"Microbial competition between Bacillus subtilis and Staphylococcus aureus monitored by imaging mass spectrometry","volume":"157","author":"Gonzalez","year":"2011","journal-title":"Microbiology"},{"key":"10.1016\/j.peptides.2012.07.021_bib0090","doi-asserted-by":"crossref","first-page":"1257L 1259","DOI":"10.1126\/science.1165265","article-title":"Antimicrobial defense and persistent infection in insects","volume":"322","author":"Haine","year":"2008","journal-title":"Science"},{"key":"10.1016\/j.peptides.2012.07.021_bib0095","first-page":"1872L 1881","article-title":"Strategies for the discovery and advancement of novel cationic antimicrobial peptides","volume":"8","author":"Hadley","year":"2010","journal-title":"Curr Top Med Chem"},{"key":"10.1016\/j.peptides.2012.07.021_bib0100","doi-asserted-by":"crossref","first-page":"585L 606","DOI":"10.2174\/138920309789630589","article-title":"Anionic antimicrobial peptides from eukaryotic organisms","volume":"10","author":"Harris","year":"2009","journal-title":"Curr Protein Pept Sci"},{"issue":"1","key":"10.1016\/j.peptides.2012.07.021_bib0105","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1038\/nrmicro2259","article-title":"Bacterial competition: surviving and thriving in the microbial jungle","volume":"8","author":"Hibbing","year":"2010","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.peptides.2012.07.021_bib0110","first-page":"494","article-title":"Short linear cationic antimicrobial peptides: screening, optimizing, and prediction","author":"Hilpert","year":"2007","journal-title":"Methods Mol Biol"},{"key":"10.1016\/j.peptides.2012.07.021_bib0115","first-page":"299L 308","article-title":"Internally quenched fluorogenic protease substrates: solid phase synthesis and fluorescence spectroscopy of peptides containing ortho-amino benzoyl\/dinitrophenyl groups as donor\u2013acceptor pairs","volume":"1","author":"Hirata","year":"1994","journal-title":"Lett Pept Sci"},{"key":"10.1016\/j.peptides.2012.07.021_bib0120","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1084\/jem.108.6.925","article-title":"Bactericidal action of histone","volume":"108","author":"Hirsch","year":"1958","journal-title":"J Exp Med"},{"key":"10.1016\/j.peptides.2012.07.021_bib0125","doi-asserted-by":"crossref","first-page":"4288","DOI":"10.1021\/bi972323m","article-title":"Three-dimensional solution structure of lactoferricin B, an antimicrobial peptide derived from bovine lactoferrin","volume":"37","author":"Hwang","year":"1998","journal-title":"Biochemistry"},{"issue":"6","key":"10.1016\/j.peptides.2012.07.021_bib0130","doi-asserted-by":"crossref","first-page":"1324","DOI":"10.1007\/s11095-009-9841-2","article-title":"Biodegradable nanoparticles improve oral bioavailability of amphotericin B and show reduced nephrotoxicity compared to intravenous Fungizone\u00ae","volume":"26","author":"Italia","year":"2009","journal-title":"Pharm Res"},{"key":"10.1016\/j.peptides.2012.07.021_bib0135","doi-asserted-by":"crossref","first-page":"1490L 1496","DOI":"10.1016\/j.febslet.2006.01.041","article-title":"Antifungal activity of synthetic peptide derived from halocidin, antimicrobial peptide from the tunicate, Halocynthia aurantium","volume":"580","author":"Jang","year":"2006","journal-title":"FEBS Lett"},{"issue":"4","key":"10.1016\/j.peptides.2012.07.021_bib0140","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1111\/j.1747-0285.2011.01086.x","volume":"77","author":"Jiang","year":"2011","journal-title":"Chem Biol Drug Des"},{"issue":"2","key":"10.1016\/j.peptides.2012.07.021_bib0145","doi-asserted-by":"crossref","first-page":"46","DOI":"10.18388\/abp.2003_3698","article-title":"Novel properties of antimicrobial peptides","volume":"50","author":"Kamysz","year":"2003","journal-title":"Acta Biochim Pol"},{"key":"10.1016\/j.peptides.2012.07.021_bib0150","doi-asserted-by":"crossref","first-page":"11995","DOI":"10.1021\/bi0103563","article-title":"Solution structures of the antifungal heliomicin and a selected variant with both antibacterial and antifungal activities","volume":"40","author":"Lamberty","year":"2001","journal-title":"Biochemistry"},{"key":"10.1016\/j.peptides.2012.07.021_bib0155","doi-asserted-by":"crossref","first-page":"263L 273","DOI":"10.1186\/1471-2105-8-263","article-title":"Analysis and prediction of antibacterial peptides","volume":"8","author":"Lata","year":"2007","journal-title":"BMC Bioinformatics"},{"key":"10.1016\/j.peptides.2012.07.021_bib0160","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/j.peptides.2008.12.001","article-title":"Identification and structural insights of three novel antimicrobial peptides isolated from green coconut water","volume":"30","author":"Mandal","year":"2009","journal-title":"Peptides"},{"key":"10.1016\/j.peptides.2012.07.021_bib0165","doi-asserted-by":"crossref","first-page":"1447L 1454","DOI":"10.1046\/j.1432-1327.2000.01143.x","article-title":"Structure\u2013function relationships of temporins, small antimicrobial peptides from amphibian skin","volume":"267","author":"Mangoni","year":"2000","journal-title":"Eur J Biochem"},{"key":"10.1016\/j.peptides.2012.07.021_bib0170","doi-asserted-by":"crossref","first-page":"922L 933","DOI":"10.1007\/s00018-007-6475-6","article-title":"Cationic host defense peptides: innate immune regulatory peptides as novel approach for treating infections","volume":"64","author":"Mookherjee","year":"2007","journal-title":"Cell Mol Life Sci"},{"issue":"15","key":"10.1016\/j.peptides.2012.07.021_bib0175","doi-asserted-by":"crossref","first-page":"8245","DOI":"10.1073\/pnas.150518097","article-title":"Structure\u2013activity analysis of buforin II, a histone H2A-derived antimicrobial peptide: the proline hing is responsible for the cell-penetrating ability of buforin II","volume":"97","author":"Park","year":"2000","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"11","key":"10.1016\/j.peptides.2012.07.021_bib0180","doi-asserted-by":"crossref","first-page":"6205","DOI":"10.1128\/IAI.71.11.6205-6212.2003","article-title":"Therapeutic activity of an engineered synthetic killer antiidiotypic antibody fragment against experimental mucosal and systemic candidiasis","volume":"71","author":"Polonelli","year":"2003","journal-title":"Infect Immun"},{"issue":"13","key":"10.1016\/j.peptides.2012.07.021_bib0185","doi-asserted-by":"crossref","first-page":"13018","DOI":"10.1074\/jbc.M310838200","article-title":"The NMR-derived solutions structure of a new cationic antimicrobial peptide from the skin secretion of the anuran Hyla punctata","volume":"279","author":"Prates","year":"2004","journal-title":"J Biol Chem"},{"key":"10.1016\/j.peptides.2012.07.021_bib0190","doi-asserted-by":"crossref","first-page":"5330","DOI":"10.1046\/j.1432-1327.2000.01536.x","article-title":"The antibiotic and anticancer active aurein peptides from the Australian bell frogs Litoria aurea and Litoria raniformis the solution structure of aurein 1.2","volume":"267","author":"Rozek","year":"2000","journal-title":"Eur J Biochem"},{"key":"10.1016\/j.peptides.2012.07.021_bib0195","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1002\/j.1460-2075.1991.tb07932.x","article-title":"The andropin gene and its product, a male-specific antibacterial peptide in Drosophila melanogaster","volume":"10","author":"Samakovlis","year":"1991","journal-title":"EMBO J"},{"key":"10.1016\/j.peptides.2012.07.021_bib0200","doi-asserted-by":"crossref","first-page":"11956","DOI":"10.1016\/S0021-9258(17)32666-2","article-title":"Antimicrobial peptides from skin secretions of Rana esculenta. Molecular cloning of cDNAs encoding esculentin and brevinins and isolation of new active peptides","volume":"269","author":"Simmaco","year":"1994","journal-title":"J Biol Chem"},{"key":"10.1016\/j.peptides.2012.07.021_bib0205","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/0014-5793(93)81299-F","article-title":"A new family of basic cysteine-rich plant antifungal proteins from Brassicaceae species","volume":"316","author":"Terras","year":"1993","journal-title":"FEBS Lett"},{"key":"10.1016\/j.peptides.2012.07.021_bib0210","doi-asserted-by":"crossref","first-page":"956L 958","DOI":"10.1093\/jac\/dkh430","article-title":"Therapeutic activity of a killer peptide against experimental paracoccidioidomycosis","volume":"54","author":"Travassos","year":"2004","journal-title":"J Antimicrob Chemother"},{"issue":"6","key":"10.1016\/j.peptides.2012.07.021_bib0215","doi-asserted-by":"crossref","first-page":"1378","DOI":"10.1016\/j.molimm.2009.11.025","article-title":"Isoleucine\/leucine2 is essential for chemoattractant activity of beta-defensin Defb14 through chemokine receptor 6","volume":"47","author":"Tyrrell","year":"2010","journal-title":"Mol Immunol"},{"issue":"9","key":"10.1016\/j.peptides.2012.07.021_bib0220","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1046\/j.1432-1033.2003.03584.x","article-title":"Antimicrobial peptides from hylid and ranin frogs originated from a 150-million-year-old ancestral precursor with a conserved signal peptide but a hypermutable antimicrobial domain","volume":"270","author":"Vanhoye","year":"2003","journal-title":"Eur J Biochem"},{"key":"10.1016\/j.peptides.2012.07.021_bib0225","doi-asserted-by":"crossref","first-page":"2194","DOI":"10.1016\/j.bpj.2008.11.063","article-title":"Structure and membrane interactions of the antibiotic peptide dermadistinctin K by multidimensional solution and oriented 15N and 31P solid-state NMR spectroscopy","volume":"96","author":"Verly","year":"2009","journal-title":"Biophys J"},{"key":"10.1016\/j.peptides.2012.07.021_bib0230","doi-asserted-by":"crossref","first-page":"D590","DOI":"10.1093\/nar\/gkh025","article-title":"APD: the antimicrobial peptide database","volume":"32","author":"Wang","year":"2004","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"10.1016\/j.peptides.2012.07.021_bib0235","first-page":"D933","article-title":"APD2: the updated antimicrobial peptide database and its application in peptide design","volume":"37","author":"Wang","year":"2008","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.peptides.2012.07.021_bib0240","doi-asserted-by":"crossref","first-page":"5207","DOI":"10.1093\/emboj\/20.18.5207","article-title":"Structure of the yeast nucleosome core particle reveals fundamental changes in internucleosome interactions","volume":"20","author":"White","year":"2001","journal-title":"EMBO J"},{"key":"10.1016\/j.peptides.2012.07.021_bib0245","first-page":"303","article-title":"In vitro activity of synthetic antimicrobial peptides against Candida","volume":"55","author":"Wojciech k Piotr","year":"2006","journal-title":"Pol Soc Microbiol"},{"key":"10.1016\/j.peptides.2012.07.021_bib0250","doi-asserted-by":"crossref","first-page":"2998","DOI":"10.1021\/bi00126a022","article-title":"Binding of tachyplesin I to DNA revealed by footprinting analysis: significant contribution of secondary structure to DNA binding and implication for biological action","volume":"31","author":"Yonezawa","year":"1992","journal-title":"Biochemistry"},{"key":"10.1016\/j.peptides.2012.07.021_bib0255","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1046\/j.1365-2796.2003.01228.x","volume":"254","author":"Boman","year":"2003","journal-title":"J Int Med"}],"container-title":["Peptides"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0196978112003221?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0196978112003221?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,1,25]],"date-time":"2022-01-25T08:51:37Z","timestamp":1643100697000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0196978112003221"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10]]},"references-count":51,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,10]]}},"alternative-id":["S0196978112003221"],"URL":"https:\/\/doi.org\/10.1016\/j.peptides.2012.07.021","relation":{},"ISSN":["0196-9781"],"issn-type":[{"value":"0196-9781","type":"print"}],"subject":[],"published":{"date-parts":[[2012,10]]}}}