{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T19:34:22Z","timestamp":1772480062313,"version":"3.50.1"},"reference-count":48,"publisher":"Elsevier BV","content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Peptides"],"published-print":{"date-parts":[[2014,4]]},"DOI":"10.1016\/j.peptides.2014.01.001","type":"journal-article","created":{"date-parts":[[2014,1,10]],"date-time":"2014-01-10T06:20:35Z","timestamp":1389334835000},"page":"9-18","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":14,"special_numbering":"C","title":["Molecular cloning, bioinformatics analysis and functional characterization of HWTX-XI toxin superfamily from the spider Ornithoctonus huwena"],"prefix":"10.1016","volume":"54","author":[{"given":"Liping","family":"Jiang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meichun","family":"Deng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhigui","family":"Duan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Songping","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.peptides.2014.01.001_bib0005","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/j.ympev.2006.07.018","article-title":"Utility of the nuclear protein-coding gene, elongation factor-1 gamma (EF-1gamma), for spider systematics, emphasizing family level relationships of tarantulas and their kin (Araneae: Mygalomorphae)","volume":"42","author":"Ayoub","year":"2007","journal-title":"Mol Phylogenet Evol"},{"key":"10.1016\/j.peptides.2014.01.001_bib0010","doi-asserted-by":"crossref","first-page":"23766","DOI":"10.1074\/jbc.C500064200","article-title":"Conkunitzin-S1 is the first member of a new Kunitz-type neurotoxin family. Structural and functional characterization","volume":"280","author":"Bayrhuber","year":"2005","journal-title":"J Biol Chem"},{"key":"10.1016\/j.peptides.2014.01.001_bib0015","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1016\/j.cell.2010.03.052","article-title":"A bivalent tarantula toxin activates the capsaicin receptor, TRPV1, by targeting the outer pore domain","volume":"141","author":"Bohlen","year":"2010","journal-title":"Cell"},{"key":"10.1016\/j.peptides.2014.01.001_bib0020","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1016\/j.peptides.2009.02.018","article-title":"Expression and characterization of jingzhaotoxin-34, a novel neurotoxin from the venom of the tarantula Chilobrachys jingzhao","volume":"30","author":"Chen","year":"2009","journal-title":"Peptides"},{"key":"10.1016\/j.peptides.2014.01.001_bib0025","doi-asserted-by":"crossref","first-page":"13813","DOI":"10.1074\/jbc.M112.343996","article-title":"Hg1, novel peptide inhibitor specific for Kv1.3 channels from first scorpion Kunitz-type potassium channel toxin family","volume":"287","author":"Chen","year":"2012","journal-title":"J Biol Chem"},{"key":"10.1016\/j.peptides.2014.01.001_bib0030","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.ejphar.2007.12.014","article-title":"Inhibition of insect calcium channels by huwentoxin-V, a neurotoxin from Chinese tarantula Ornithoctonus huwena venom","volume":"582","author":"Deng","year":"2008","journal-title":"Eur J Pharmacol"},{"key":"10.1016\/j.peptides.2014.01.001_bib0035","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1586\/epr.09.45","article-title":"Venomics as a drug discovery platform","volume":"6","author":"Escoubas","year":"2009","journal-title":"Expert Rev Proteomics"},{"key":"10.1016\/j.peptides.2014.01.001_bib0040","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1002\/jms.1389","article-title":"Venomics: unravelling the complexity of animal venoms with mass spectrometry","volume":"43","author":"Escoubas","year":"2008","journal-title":"J Mass Spectrom"},{"key":"10.1016\/j.peptides.2014.01.001_bib0045","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1016\/j.toxicon.2006.01.018","article-title":"Venom landscapes: mining the complexity of spider venoms via a combined cDNA and mass spectrometric approach","volume":"47","author":"Escoubas","year":"2006","journal-title":"Toxicon"},{"key":"10.1016\/j.peptides.2014.01.001_bib0050","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.peptides.2013.02.009","article-title":"Purification, characterization and molecular cloning of chymotrypsin inhibitor peptides from the venom of Burmese Daboia russelii siamensis","volume":"43C","author":"Guo","year":"2013","journal-title":"Peptides"},{"key":"10.1016\/j.peptides.2014.01.001_bib0055","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/S0306-3623(96)00173-5","article-title":"Recent studies on dendrotoxins and potassium ion channels","volume":"28","author":"Harvey","year":"1997","journal-title":"Gen Pharmacol"},{"key":"10.1016\/j.peptides.2014.01.001_bib0060","doi-asserted-by":"crossref","first-page":"D653","DOI":"10.1093\/nar\/gkq1058","article-title":"ArachnoServer 2.0, an updated online resource for spider toxin sequences and structures","volume":"39","author":"Herzig","year":"2011","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.peptides.2014.01.001_bib0065","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.1016\/j.peptides.2008.06.001","article-title":"Genomic organization and cloning of novel genes encoding toxin-like peptides of three superfamilies from the spider Orinithoctonus huwena","volume":"29","author":"Jiang","year":"2008","journal-title":"Peptides"},{"key":"10.1016\/j.peptides.2014.01.001_bib0070","doi-asserted-by":"crossref","first-page":"1479","DOI":"10.1016\/j.toxicon.2008.03.024","article-title":"Molecular diversification based on analysis of expressed sequence tags from the venom glands of the Chinese bird spider Ornithoctonus huwena","volume":"51","author":"Jiang","year":"2008","journal-title":"Toxicon"},{"key":"10.1016\/j.peptides.2014.01.001_bib0075","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1016\/j.peptides.2008.12.012","article-title":"Expression, purification and characterization of a group of lectin-like peptides from the spider Ornithoctonus huwena","volume":"30","author":"Jiang","year":"2009","journal-title":"Peptides"},{"key":"10.1016\/j.peptides.2014.01.001_bib0080","doi-asserted-by":"crossref","first-page":"100","DOI":"10.4161\/chan.2.2.6022","article-title":"Peptide toxins that selectively target insect Na(V) and Ca(V) channels","volume":"2","author":"King","year":"2008","journal-title":"Channels (Austin)"},{"key":"10.1016\/j.peptides.2014.01.001_bib0085","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1146\/annurev-ento-120811-153650","article-title":"Spider-venom peptides: structure, pharmacology, and potential for control of insect pests","volume":"58","author":"King","year":"2013","journal-title":"Annu Rev Entomol"},{"key":"10.1016\/j.peptides.2014.01.001_bib0090","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1006\/jmbi.1999.2757","article-title":"Interscaffolding additivity: binding of P1 variants of bovine pancreatic trypsin inhibitor to four serine proteases","volume":"289","author":"Krowarsch","year":"1999","journal-title":"J Mol Biol"},{"key":"10.1016\/j.peptides.2014.01.001_bib0095","doi-asserted-by":"crossref","first-page":"2947","DOI":"10.1093\/bioinformatics\/btm404","article-title":"Clustal W and Clustal X version 2.0","volume":"23","author":"Larkin","year":"2007","journal-title":"Bioinformatics"},{"key":"10.1016\/j.peptides.2014.01.001_bib0100","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1146\/annurev.bi.49.070180.003113","article-title":"Protein inhibitors of proteinases","volume":"49","author":"Laskowski","year":"1980","journal-title":"Annu Rev Biochem"},{"key":"10.1016\/j.peptides.2014.01.001_bib0105","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/j.toxicon.2004.02.005","article-title":"An overview of peptide toxins from the venom of the Chinese bird spider Selenocosmia huwena Wang [=Ornithoctonus huwena (Wang)]","volume":"43","author":"Liang","year":"2004","journal-title":"Toxicon"},{"key":"10.1016\/j.peptides.2014.01.001_bib0110","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1586\/14789450.5.5.731","article-title":"Proteome and peptidome profiling of spider venoms","volume":"5","author":"Liang","year":"2008","journal-title":"Expert Rev Proteomics"},{"key":"10.1016\/j.peptides.2014.01.001_bib0115","doi-asserted-by":"crossref","first-page":"8628","DOI":"10.1074\/jbc.M513542200","article-title":"Function and solution structure of Huwentoxin-X, a specific blocker of N-type calcium channels, from the Chinese bird spider Ornithoctonus huwena","volume":"281","author":"Liu","year":"2006","journal-title":"J Biol Chem"},{"key":"10.1016\/j.peptides.2014.01.001_bib0120","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.peptides.2007.11.013","article-title":"A novel serine protease inhibitor from Bungarus fasciatus venom","volume":"29","author":"Lu","year":"2008","journal-title":"Peptides"},{"key":"10.1016\/j.peptides.2014.01.001_bib0125","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1023\/A:1020663619657","article-title":"Three-dimensional structure of Selenocosmia huwena lectin-I (SHL-I) from the venom of the spider Selenocosmia huwena by 2D-NMR","volume":"18","author":"Lu","year":"1999","journal-title":"J Protein Chem"},{"key":"10.1016\/j.peptides.2014.01.001_bib0130","doi-asserted-by":"crossref","first-page":"e61049","DOI":"10.1371\/journal.pone.0061049","article-title":"Beneficial effects of trypsin inhibitors derived from a spider venom peptide in l-arginine-induced severe acute pancreatitis in mice","volume":"8","author":"Ning","year":"2013","journal-title":"PLoS ONE"},{"key":"10.1016\/j.peptides.2014.01.001_bib0135","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1186\/1471-2199-8-32","article-title":"Selection strategy and the design of hybrid oligonucleotide primers for RACE-PCR: cloning a family of toxin-like sequences from Agelena orientalis","volume":"8","author":"Pan","year":"2007","journal-title":"BMC Mol Biol"},{"key":"10.1016\/j.peptides.2014.01.001_bib0140","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.bcp.2011.03.023","article-title":"A bifunctional sea anemone peptide with Kunitz type protease and potassium channel inhibiting properties","volume":"82","author":"Peigneur","year":"2011","journal-title":"Biochem Pharmacol"},{"key":"10.1016\/j.peptides.2014.01.001_bib0145","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/S0041-0101(00)00150-1","article-title":"The effect of Huwentoxin-I on Ca(2+) channels in differentiated NG108-15 cells, a patch-clamp study","volume":"39","author":"Peng","year":"2001","journal-title":"Toxicon"},{"key":"10.1016\/j.peptides.2014.01.001_bib0150","doi-asserted-by":"crossref","first-page":"47564","DOI":"10.1074\/jbc.M204063200","article-title":"Function and solution structure of huwentoxin-IV, a potent neuronal tetrodotoxin (TTX)-sensitive sodium channel antagonist from Chinese bird spider Selenocosmia huwena","volume":"277","author":"Peng","year":"2002","journal-title":"J Biol Chem"},{"key":"10.1016\/j.peptides.2014.01.001_bib0155","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1038\/nmeth.1701","article-title":"SignalP 4.0: discriminating signal peptides from transmembrane regions","volume":"8","author":"Petersen","year":"2011","journal-title":"Nat Methods"},{"key":"10.1016\/j.peptides.2014.01.001_bib0160","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1016\/j.toxicon.2011.09.015","article-title":"Scorpion and spider venom peptides: gene cloning and peptide expression","volume":"58","author":"Quintero-Hernandez","year":"2011","journal-title":"Toxicon"},{"key":"10.1016\/j.peptides.2014.01.001_bib0165","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.dci.2012.10.005","article-title":"Structure and function of invertebrate Kunitz serine protease inhibitors","volume":"39","author":"Ranasinghe","year":"2013","journal-title":"Dev Comp Immunol"},{"key":"10.1016\/j.peptides.2014.01.001_bib0170","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.1111\/j.1538-7836.2006.01969.x","article-title":"Molecular evolution caught in action: gene duplication and evolution of molecular isoforms of prothrombin activators in Pseudonaja textilis (brown snake)","volume":"4","author":"Reza","year":"2006","journal-title":"J Thromb Haemost"},{"key":"10.1016\/j.peptides.2014.01.001_bib0175","doi-asserted-by":"crossref","first-page":"2851","DOI":"10.3390\/toxins2122851","article-title":"Spider-venom peptides as therapeutics","volume":"2","author":"Saez","year":"2010","journal-title":"Toxins"},{"key":"10.1016\/j.peptides.2014.01.001_bib0180","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1016\/j.biochi.2004.09.010","article-title":"Boophilus microplus tick larvae, a rich source of Kunitz type serine proteinase inhibitors","volume":"86","author":"Sasaki","year":"2004","journal-title":"Biochimie"},{"key":"10.1016\/j.peptides.2014.01.001_bib0185","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.regpep.2005.09.007","article-title":"Cloning of novel bombesin precursor cDNAs from skin of Bombina maxima","volume":"132","author":"Shen","year":"2005","journal-title":"Regul Pept"},{"key":"10.1016\/j.peptides.2014.01.001_bib0190","doi-asserted-by":"crossref","first-page":"2731","DOI":"10.1093\/molbev\/msr121","article-title":"MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods","volume":"28","author":"Tamura","year":"2011","journal-title":"Mol Biol Evol"},{"key":"10.1016\/j.peptides.2014.01.001_bib0195","doi-asserted-by":"crossref","first-page":"2550","DOI":"10.1021\/pr1000016","article-title":"Molecular diversification of peptide toxins from the tarantula Haplopelma hainanum (Ornithoctonus hainana) venom based on transcriptomic, peptidomic, and genomic analyses","volume":"9","author":"Tang","year":"2010","journal-title":"J Proteome Res"},{"key":"10.1016\/j.peptides.2014.01.001_bib0200","doi-asserted-by":"crossref","first-page":"e53343","DOI":"10.1371\/journal.pone.0053343","article-title":"A spider-derived Kunitz-type serine protease inhibitor that acts as a plasmin inhibitor and an elastase inhibitor","volume":"8","author":"Wan","year":"2013","journal-title":"PLoS ONE"},{"key":"10.1016\/j.peptides.2014.01.001_bib0205","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/j.bbrc.2007.02.168","article-title":"The cross channel activities of spider neurotoxin huwentoxin-I on rat dorsal root ganglion neurons","volume":"357","author":"Wang","year":"2007","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1016\/j.peptides.2014.01.001_bib0210","doi-asserted-by":"crossref","first-page":"191","DOI":"10.3390\/toxins4030191","article-title":"Spider-venom peptides as bioinsecticides","volume":"4","author":"Windley","year":"2012","journal-title":"Toxins"},{"key":"10.1016\/j.peptides.2014.01.001_bib0215","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.gene.2012.01.009","article-title":"Venom evolution through gene duplications","volume":"496","author":"Wong","year":"2012","journal-title":"Gene"},{"key":"10.1016\/j.peptides.2014.01.001_bib0220","doi-asserted-by":"crossref","first-page":"2844","DOI":"10.1021\/bi011367z","article-title":"Gene expression, mutation, and structure-function relationship of scorpion toxin BmP05 active on SK(Ca) channels","volume":"41","author":"Wu","year":"2002","journal-title":"Biochemistry"},{"key":"10.1016\/j.peptides.2014.01.001_bib0225","doi-asserted-by":"crossref","first-page":"27300","DOI":"10.1074\/jbc.M708447200","article-title":"Tarantula huwentoxin-IV inhibits neuronal sodium channels by binding to receptor site 4 and trapping the domain ii voltage sensor in the closed configuration","volume":"283","author":"Xiao","year":"2008","journal-title":"J Biol Chem"},{"key":"10.1016\/j.peptides.2014.01.001_bib0230","doi-asserted-by":"crossref","first-page":"2792","DOI":"10.1021\/pr0700192","article-title":"Proteomic and peptidomic characterization of the venom from the Chinese bird spider, Ornithoctonus huwena Wang","volume":"6","author":"Yuan","year":"2007","journal-title":"J Proteome Res"},{"key":"10.1016\/j.peptides.2014.01.001_bib0235","doi-asserted-by":"crossref","first-page":"e3414","DOI":"10.1371\/journal.pone.0003414","article-title":"Discovery of a distinct superfamily of Kunitz-type toxin (KTT) from tarantulas","volume":"3","author":"Yuan","year":"2008","journal-title":"PLoS ONE"},{"key":"10.1016\/j.peptides.2014.01.001_bib0240","doi-asserted-by":"crossref","first-page":"e27548","DOI":"10.1371\/journal.pone.0027548","article-title":"SdPI, the first functionally characterized Kunitz-type trypsin inhibitor from scorpion venom","volume":"6","author":"Zhao","year":"2011","journal-title":"PLoS ONE"}],"container-title":["Peptides"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0196978114000035?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0196978114000035?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,10,7]],"date-time":"2018-10-07T07:55:20Z","timestamp":1538898920000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0196978114000035"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4]]},"references-count":48,"alternative-id":["S0196978114000035"],"URL":"https:\/\/doi.org\/10.1016\/j.peptides.2014.01.001","relation":{},"ISSN":["0196-9781"],"issn-type":[{"value":"0196-9781","type":"print"}],"subject":[],"published":{"date-parts":[[2014,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular cloning, bioinformatics analysis and functional characterization of HWTX-XI toxin superfamily from the spider Ornithoctonus huwena","name":"articletitle","label":"Article Title"},{"value":"Peptides","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.peptides.2014.01.001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}