{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T23:28:37Z","timestamp":1790292517900,"version":"4.1.0"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["104-2313-B-005-017-"],"award-info":[{"award-number":["104-2313-B-005-017-"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Pesticide Biochemistry and Physiology"],"published-print":{"date-parts":[[2018,6]]},"DOI":"10.1016\/j.pestbp.2018.03.016","type":"journal-article","created":{"date-parts":[[2018,3,28]],"date-time":"2018-03-28T13:13:35Z","timestamp":1522242815000},"page":"68-73","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":17,"special_numbering":"C","title":["Nicotinic acetylcholine receptor subunit \u03b16 associated with spinosad resistance in Rhyzopertha dominica (Coleoptera: Bostrichidae)"],"prefix":"10.1016","volume":"148","author":[{"given":"Hang-Tang","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4319-686X","authenticated-orcid":false,"given":"Cheng-Lung","family":"Tsai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei-Er","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.pestbp.2018.03.016_bb0005","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jspr.2011.08.007","article-title":"A review of the biology and control of Rhyzopertha dominica (F.) the lesser grain borer","volume":"48","author":"Edde","year":"2012","journal-title":"J. Stored Prod. Res."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0010","series-title":"Arthropod Pesticide Resistance Database","author":"APRD","year":"2017"},{"key":"10.1016\/j.pestbp.2018.03.016_bb0015","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1002\/1526-4998(200008)56:8<696::AID-PS182>3.0.CO;2-5","article-title":"Spinosad - a case study: an example from a natural products discovery programme","volume":"56","author":"Thompson","year":"2000","journal-title":"Pest Manag. Sci."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0020","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1603\/0022-0493-95.3.640","article-title":"Effectiveness of spinosad on four classes of wheat against five stored-product insects","volume":"95","author":"Fang","year":"2002","journal-title":"J. Econ. Entomol."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0025","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1002\/ps.660","article-title":"Contribution of contact toxicity and wheat condition to mortality of stored-product insects exposed to spinosad","volume":"59","author":"Toews","year":"2003","journal-title":"Pest Manag. Sci."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0030","doi-asserted-by":"crossref","first-page":"2154","DOI":"10.1093\/jee\/97.6.2154","article-title":"Susceptibility of laboratory and field strains of four stored-product insect species to spinosad","volume":"97","author":"Huang","year":"2004","journal-title":"J. Econ. Entomol."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0035","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1002\/ps.1141","article-title":"Long-term persistence and efficacy of spinosad against Rhyxopertha dominica (Coleoptera: Bostrychidae) in wheat","volume":"62","author":"Daglish","year":"2006","journal-title":"Pest Manag. Sci."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0040","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1603\/0022-0493(2008)101[976:EOTACO]2.0.CO;2","article-title":"Effect of temperature and commodity on insecticidal efficacy of spinosad dust against Sitophilus oryzae (Coleoptera: Curculionidae) and Rhyzopertha dominica (Coleoptera: Bostrychidae)","volume":"101","author":"Athanassiou","year":"2008","journal-title":"J. Econ. Entomol."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0045","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1603\/0022-0493(2008)101[1022:IADMOR]2.0.CO;2","article-title":"Immediate and delayed mortality of Rhyzopetha dominica (Coleoptera: Bostrichidae) and Sitophilus oryzae (Coleoptera: Curculionidae) adults exposed to spinosad-treated commodities","volume":"101","author":"Getchell","year":"2008","journal-title":"J. Econ. Entomol."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0050","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/j.cropro.2009.01.006","article-title":"Activity of spinosad against three sotred-product beetle species on four grain commodities","volume":"28","author":"Vayias","year":"2009","journal-title":"Crop Prot."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0055","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1603\/EC09115","article-title":"Effects of short exposures to spinosad-treated wheat or maize on four stored-grain insects","volume":"103","author":"Athanassiou","year":"2010","journal-title":"J. Econ. Entomol."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0060","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/s10340-011-0395-9","article-title":"Performance of pre-commercial release formulations of spinosad against five stored-product insect species on four stored commodities","volume":"85","author":"Subramanyam","year":"2012","journal-title":"J. Pest. Sci."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0065","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/j.jspr.2004.07.002","article-title":"Effectiveness of spinosad as a grain protectant against resistant beetle and psocid pests of stored grain in Australia","volume":"41","author":"Nayak","year":"2005","journal-title":"J. Stored Prod. Res."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0070","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.jspr.2011.01.004","article-title":"Spinosad: a new natural product for stored grain protection","volume":"47","author":"Hertlein","year":"2011","journal-title":"J. Stored Prod. Res."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0075","doi-asserted-by":"crossref","first-page":"2208","DOI":"10.1603\/EC13215","article-title":"Efficacies of spinosad and a combination of chlorpyrifos-methyl and deltamerthrin against phosphine-resistant Rhyzopertha dominica (Coleoptera: Bostrichidae) and Tribolium castaneum (Coleoptera: Tenebrionidae) on wheat","volume":"106","author":"Bajracharya","year":"2013","journal-title":"J. Econ. Entomol."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0080","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.jspr.2013.10.001","article-title":"Susceptibility of field populations of the lesser grain borer, Rhyzopertha dominica (F.), to deltamethrin and spinosad on paddy rice in Taiwan","volume":"55","author":"Chen","year":"2013","journal-title":"J. Stored Prod. Res."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0085","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.pestbp.2011.11.004","article-title":"Resistance and cross-resistance to spinosyns - a review and analysis","volume":"102","author":"Sparks","year":"2012","journal-title":"Pestic. Biochem. Physiol."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0090","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1000802","article-title":"Mis-spliced transcripts of nicotinic acetylcholine receptor \u03b16 are associated with field evolved spinosad resistance in Plutella xylostella (L.)","volume":"6","author":"Baxter","year":"2010","journal-title":"PLoS Genet."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0095","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/s10158-010-0102-1","article-title":"Transcripts of the nicotinic acetylcholine receptor subunit gene Pxyl\u03b16 with premature stop codons are associated with spinosad resistance in diamondback moth, Plutella xylostella","volume":"10","author":"Rinkevich","year":"2010","journal-title":"Invertebr. Neurosci."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0100","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1016\/j.ibmb.2012.07.010","article-title":"Truncated transcripts of nicotinic acetylcholine subunit gene Bd\u03b16 are associated with spinosad resistance in Bactrocera dorsalis","volume":"42","author":"Hsu","year":"2012","journal-title":"Insect Biochem. Mol. Biol."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0105","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1111\/jnc.12029","article-title":"A nicotinic acetylcholine receptor transmembrane point mutation (G275E) associated with resistance to spinosad in Frankliniella occidentalis","volume":"124","author":"Puinean","year":"2013","journal-title":"J. Neurochem."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0110","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.pestbp.2014.04.013","article-title":"Spinosad resistance of melon thrips, Thrips palmi, is conferred by G275E mutation in \u03b16 subunit of nicotinic acetylcholine receptor and cytochrome P450 detoxification","volume":"112","author":"Bao","year":"2014","journal-title":"Pestic. Biochem. Physiol."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0115","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.pestbp.2016.02.006","article-title":"Mutation (G275E) of the nicotinic acetylcholine receptor \u03b16 subunit is associated with high levels of resistance to spinosyns in Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)","volume":"131","author":"Silva","year":"2016","journal-title":"Pestic. Biochem. Physiol."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0120","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0170935","article-title":"Analysis of differentially expressed genes related to resistance in spinosad- and neonicotinoid-resistant Musca domestica L. (Diptera: Muscidae) strains","volume":"12","author":"H\u00f8jland","year":"2017","journal-title":"PLoS ONE"},{"key":"10.1016\/j.pestbp.2018.03.016_bb0125","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.pestbp.2011.10.008","article-title":"Metabolic mechanisms involved in the resistance of field populations of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) to spinosad","volume":"102","author":"Reyes","year":"2012","journal-title":"Pestic. Biochem. Physiol."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0130","series-title":"Probit Analysis","author":"Finney","year":"1971"},{"key":"10.1016\/j.pestbp.2018.03.016_bb0135","series-title":"Probit-MSChart: A Computer Program for Probit Analysis","author":"Chi","year":"2009"},{"key":"10.1016\/j.pestbp.2018.03.016_bb0140","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1186\/1471-2164-8-324","article-title":"The nicotinic acetylcholine receptor gene family of the silkworm, Bombyx mori","volume":"8","author":"Shao","year":"2007","journal-title":"BMC Genomics"},{"issue":"2","key":"10.1016\/j.pestbp.2018.03.016_bb0145","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1111\/j.1365-2583.2009.00873.x","article-title":"Transcriptional diversity and allelic variation in nicotinic acetylcholine receptor subunits of the red flour beetle, Tribolium castaneum","author":"Rinkevich","year":"2009","journal-title":"Insect Biochem. Mol. Biol."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0150","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1186\/1471-2148-7-98","article-title":"RNA editing and alternative splicing of the insect nAChR subunit alpha6 transcript: evolutionary conservation, divergence and regulation","volume":"7","author":"Jin","year":"2007","journal-title":"BMC Evol. Biol."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0155","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.ygeno.2004.09.001","article-title":"The nicotinic acetylcholine receptor gene family of the malaria mosquito, Anopheles gambiae","volume":"85","author":"Jones","year":"2005","journal-title":"Genomics"},{"key":"10.1016\/j.pestbp.2018.03.016_bb0160","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1038\/nmeth.1701","article-title":"SignalP 4.0: discriminating signal peptides from transmembrane regions","volume":"8","author":"Petersen","year":"2011","journal-title":"Nat. Methods"},{"key":"10.1016\/j.pestbp.2018.03.016_bb0165","first-page":"166","article-title":"TMbase - a database of membrane spanning proteins segments","volume":"374","author":"Hofmann","year":"1993","journal-title":"Biol. Chem. Hoppe Seyler"},{"key":"10.1016\/j.pestbp.2018.03.016_bb0170","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1093\/molbev\/msp259","article-title":"SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building","volume":"27","author":"Gouy","year":"2010","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0175","doi-asserted-by":"crossref","first-page":"2725","DOI":"10.1093\/molbev\/mst197","article-title":"MEGA6: molecular evolutionary genetics analysis version 6.0","volume":"30","author":"Tamura","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0180","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0103235","article-title":"Spinosad and the tomato borer Tuta absoluta: a bioinsecticide, an invasive pest threat, and high insecticide resistance","volume":"9","author":"Campos","year":"2014","journal-title":"PLoS ONE"},{"key":"10.1016\/j.pestbp.2018.03.016_bb0185","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1093\/jee\/99.3.931","article-title":"Development of resistance to spinosad in oriental fruit fly (Diptera: Tephritidae) in laboratory selection and cross-resistance","volume":"99","author":"Hsu","year":"2006","journal-title":"J. Econ. Entomol."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0190","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1038\/335645a0","article-title":"Rings of negatively charged amino acids determine the acetylcholine receptor channel conductance","volume":"335","author":"Imoto","year":"1988","journal-title":"Nature"},{"key":"10.1016\/j.pestbp.2018.03.016_bb0195","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1080\/09687680802035675","article-title":"Assembly and trafficking of nicotinic acetylcholine receptors (review)","volume":"25","author":"Millar","year":"2008","journal-title":"Mol. Membr. Biol."},{"key":"10.1016\/j.pestbp.2018.03.016_bb0200","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.ibmb.2015.01.018","article-title":"In vivo functional analysis of the Drosophila melanogaster nicotinic acetylcholine receptor D\u03b16 using the insecticide spinosad","volume":"64","author":"Somers","year":"2015","journal-title":"Insect Biochem. Mol. Biol."}],"container-title":["Pesticide Biochemistry and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0048357517304595?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0048357517304595?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T17:56:30Z","timestamp":1758650190000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0048357517304595"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6]]},"references-count":40,"alternative-id":["S0048357517304595"],"URL":"https:\/\/doi.org\/10.1016\/j.pestbp.2018.03.016","relation":{},"ISSN":["0048-3575"],"issn-type":[{"value":"0048-3575","type":"print"}],"subject":[],"published":{"date-parts":[[2018,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Nicotinic acetylcholine receptor subunit \u03b16 associated with spinosad resistance in Rhyzopertha dominica (Coleoptera: Bostrichidae)","name":"articletitle","label":"Article Title"},{"value":"Pesticide Biochemistry and Physiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.pestbp.2018.03.016","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}