{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T17:24:24Z","timestamp":1763400264797,"version":"3.38.0"},"reference-count":55,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004374","name":"Chongqing Medical University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004374","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31872290"],"award-info":[{"award-number":["31872290"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Pesticide Biochemistry and Physiology"],"published-print":{"date-parts":[[2025,3]]},"DOI":"10.1016\/j.pestbp.2024.106261","type":"journal-article","created":{"date-parts":[[2024,12,24]],"date-time":"2024-12-24T17:22:13Z","timestamp":1735060933000},"page":"106261","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["A streamlined cloning and toxicity evaluation strategy of toxin-antitoxin systems in insect cells"],"prefix":"10.1016","volume":"208","author":[{"given":"Yunfei","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shizhe","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liping","family":"Lv","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueting","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanbing","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weihua","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongxia","family":"Hua","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhihui","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.pestbp.2024.106261_bb0005","doi-asserted-by":"crossref","first-page":"6059","DOI":"10.1073\/pnas.93.12.6059","article-title":"An Escherichia coli chromosomal \u201caddiction module\u201d regulated by guanosine [corrected] 3\u2019,5\u2019-bispyrophosphate: a model for programmed bacterial cell death","volume":"193","author":"Aizenman","year":"1996","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.pestbp.2024.106261_bb0010","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1111\/mmi.13225","article-title":"An ADP-ribosyltransferase Alt of bacteriophage T4 negatively regulates the Escherichia coli\u00a0MazF toxin of a toxin-antitoxin module","volume":"99","author":"Alawneh","year":"2016","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.pestbp.2024.106261_bb0015","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s12896-015-0138-8","article-title":"Expression of the Streptococcus pneumoniae yoeB chromosomal toxin gene causes cell death in the model plant Arabidopsis thaliana","volume":"15","author":"Bakar","year":"2015","journal-title":"BMC Biotechnol."},{"key":"10.1016\/j.pestbp.2024.106261_bb0020","doi-asserted-by":"crossref","first-page":"6600","DOI":"10.1128\/JB.185.22.6600-6608.2003","article-title":"A novel family of Escherichia coli toxin-antitoxin gene pairs","volume":"185","author":"Brown","year":"2003","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.pestbp.2024.106261_bb0025","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1038\/nchembio.1364","article-title":"The fic protein doc uses an inverted substrate to phosphorylate and inactivate EF-Tu","volume":"9","author":"Castro-Roa","year":"2013","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.pestbp.2024.106261_bb0035","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1089\/hum.2010.001","article-title":"Acquisition of HIV-1 resistance in T lymphocytes using an ACA-specific E. Coli mRNA interferase","volume":"2","author":"Chono","year":"2011","journal-title":"Hum. Gene Ther."},{"key":"10.1016\/j.pestbp.2024.106261_bb0040","doi-asserted-by":"crossref","first-page":"2205","DOI":"10.1021\/bi902133s","article-title":"A conserved mode of protein recognition and binding in a ParD-ParE toxin-antitoxin complex","volume":"49","author":"Dalton","year":"2010","journal-title":"Biochemistry"},{"key":"10.1016\/j.pestbp.2024.106261_bb0045","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1093\/emboj\/cdg026","article-title":"Regulatable killing of eukaryotic cells by the prokaryotic proteins kid and Kis","volume":"22","author":"de la Cueva-M\u00e9ndez","year":"2003","journal-title":"EMBO J."},{"key":"10.1016\/j.pestbp.2024.106261_bb0050","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.1000317","article-title":"Ciprofloxacin causes persister formation by inducing the TisB toxin in Escherichia coli","volume":"8","author":"D\u00f6rr","year":"2010","journal-title":"PLoS Biol."},{"key":"10.1016\/j.pestbp.2024.106261_bb0055","doi-asserted-by":"crossref","first-page":"3116","DOI":"10.1073\/pnas.83.10.3116","article-title":"Unique type of plasmid maintenance function: postsegregational killing of plasmid-free cells","volume":"83","author":"Gerdes","year":"1986","journal-title":"Pro Natl Acad Sci USA"},{"key":"10.1016\/j.pestbp.2024.106261_bb0060","doi-asserted-by":"crossref","DOI":"10.1016\/j.pep.2023.106403","article-title":"Evaluation of different strategies to produce vibrio cholerae ParE2 toxin","volume":"215","author":"Girardin","year":"2024","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.pestbp.2024.106261_bb0065","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/s00438-004-1048-y","article-title":"Escherichia coli mazEF-mediated cell death as a defense mechanism that inhibits the spread of phage P1","volume":"272","author":"Hazan","year":"2004","journal-title":"Mol. Genet. Genomics"},{"key":"10.1016\/j.pestbp.2024.106261_bb0070","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/j.bbrc.2016.12.130","article-title":"The role of the loop 1 region in MazFbs mRNA interferase from Bacillus subtilis in recognition of the 3\u2032 end of the RNA substrate","volume":"483","author":"Ishida","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.pestbp.2024.106261_bb0075","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1128\/jb.163.3.841-849.1985","article-title":"Effects of the ccd function of the F plasmid on bacterial growth","volume":"163","author":"Jaffe","year":"1985","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.pestbp.2024.106261_bb0080","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/s41579-021-00661-1","article-title":"Biology and evolution of bacterial toxin-antitoxin systems","volume":"20","author":"Jur\u0117nas","year":"2022","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.pestbp.2024.106261_bb0085","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1016\/S1097-2765(03)00097-2","article-title":"Crystal structure of the MazE\/MazF complex: molecular bases of antidote-toxin recognition","volume":"11","author":"Kamada","year":"2003","journal-title":"Mol. Cell"},{"key":"10.1016\/j.pestbp.2024.106261_bb0090","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1534\/genetics.110.121798","article-title":"Escherichia coli rnlA and rnlB compose a novel toxin-antitoxin system","volume":"187","author":"Koga","year":"2011","journal-title":"Genetics"},{"key":"10.1016\/j.pestbp.2024.106261_bb0095","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/j.bbagen.2018.12.010","article-title":"A novel method for cloning of coding sequences of highly toxic proteins","volume":"1863","author":"Krela","year":"2019","journal-title":"Biochim. Biophys. Acta Gen. Subj."},{"key":"10.1016\/j.pestbp.2024.106261_bb0100","doi-asserted-by":"crossref","first-page":"5524","DOI":"10.1128\/AEM.66.12.5524-5526.2000","article-title":"Bacterial toxin-antitoxin gene system as containment control in yeast cells","volume":"66","author":"Kristoffersen","year":"2000","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.pestbp.2024.106261_bb0105","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1128\/JB.186.1.240-243.2004","article-title":"Identification of residues of the kid toxin involved in autoregulation of the parD system","volume":"186","author":"Lemonnier","year":"2004","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.pestbp.2024.106261_bb0110","doi-asserted-by":"crossref","first-page":"5513","DOI":"10.1093\/nar\/gkr131","article-title":"Diversity of bacterial type II toxin-antitoxin systems: a comprehensive search and functional analysis of novel families","volume":"39","author":"Leplae","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.pestbp.2024.106261_bb0115","doi-asserted-by":"crossref","first-page":"5513","DOI":"10.1093\/nar\/gkr131","article-title":"Diversity of bacterial type II toxin-antitoxin systems: a comprehensive search and functional analysis of novel families","volume":"39","author":"Leplae","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.pestbp.2024.106261_bb0120","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.ejbt.2017.10.004","article-title":"Development of a novel vector for cloning and expressing extremely toxic genes in Escherichia coli","volume":"30","author":"Li","year":"2019","journal-title":"Electron. J. Biotechnol."},{"key":"10.1016\/j.pestbp.2024.106261_bb0125","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1146\/annurev-virology-011620-040628","article-title":"Abortive infection: bacterial suicide as an antiviral immune strategy","volume":"7","author":"Lopatina","year":"2020","journal-title":"Annu Rev Virol"},{"key":"10.1016\/j.pestbp.2024.106261_bb0130","doi-asserted-by":"crossref","first-page":"e01658","DOI":"10.1128\/mBio.01658-19","article-title":"The toxin-antitoxin MazEF Drives Staphylococcus aureus biofilm formation, antibiotic tolerance, and chronic infection","volume":"10","author":"Ma","year":"2019","journal-title":"mBio"},{"key":"10.1016\/j.pestbp.2024.106261_bib276","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J Immunol Methods"},{"key":"10.1016\/j.pestbp.2024.106261_bb0135","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.1001033","article-title":"A novel mechanism of programmed cell death in bacteria by toxin-antitoxin systems corrupts peptidoglycan synthesis","volume":"9","author":"Mutschler","year":"2011","journal-title":"PLoS Biol."},{"key":"10.1016\/j.pestbp.2024.106261_bb0140","doi-asserted-by":"crossref","first-page":"1266","DOI":"10.1128\/JB.01130-06","article-title":"The yefM-yoeB toxin-antitoxin systems of Escherichia coli and Streptococcus pneumoniae: functional and structural correlation","volume":"189","author":"Nieto","year":"2007","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.pestbp.2024.106261_bb0145","doi-asserted-by":"crossref","first-page":"4784","DOI":"10.1073\/pnas.80.15.4784","article-title":"Mini-F plasmid genes that couple host cell division to plasmid proliferation","volume":"80","author":"Ogura","year":"1983","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.pestbp.2024.106261_bb0150","doi-asserted-by":"crossref","first-page":"2526","DOI":"10.1016\/j.febslet.2011.07.008","article-title":"Bacillus subtilis MazF-bs (EndoA) is a UACAU-specific mRNA interferase","volume":"585","author":"Park","year":"2011","journal-title":"FEBS Lett."},{"key":"10.1016\/j.pestbp.2024.106261_bb0155","doi-asserted-by":"crossref","first-page":"3794","DOI":"10.1128\/AEM.00364-12","article-title":"Intramolecular regulation of the sequence-specific mRNA interferase activity of MazF fused to a MazE fragment with a linker cleavable by specific proteases","volume":"78","author":"Park","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.pestbp.2024.106261_bb0160","doi-asserted-by":"crossref","first-page":"2044","DOI":"10.1128\/jb.178.7.2044-2050.1996","article-title":"Exclusion of T4 phage by the hok\/sok killer locus from plasmid R1","volume":"178","author":"Pecota","year":"1996","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.pestbp.2024.106261_bb0165","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/S0092-8674(02)01248-5","article-title":"The bacterial toxin RelE displays codon-specific cleavage of mRNAs in the ribosomal a site","volume":"112","author":"Pedersen","year":"2003","journal-title":"Cell"},{"key":"10.1016\/j.pestbp.2024.106261_bb0170","doi-asserted-by":"crossref","first-page":"3459","DOI":"10.1038\/sj.emboj.7600815","article-title":"Kid cleaves specific mRNAs at UUACU sites to rescue the copy number of plasmid R1","volume":"24","author":"Pimentel","year":"2005","journal-title":"EMBO J."},{"key":"10.1016\/j.pestbp.2024.106261_bb0175","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/S0147-619X(03)00048-9","article-title":"Non-cytotoxic variants of the Kid protein that retain their auto-regulatory activity","volume":"50","author":"Santos-Sierra","year":"2003","journal-title":"Plasmid"},{"key":"10.1016\/j.pestbp.2024.106261_bb0180","doi-asserted-by":"crossref","first-page":"3538","DOI":"10.1038\/ncomms4538","article-title":"An RNA-seq method for defining endoribonuclease cleavage specificity identifies dual rRNA substrates for toxin MazF-mt3","volume":"5","author":"Schifano","year":"2014","journal-title":"Nat. Commun."},{"key":"10.1016\/j.pestbp.2024.106261_bb0185","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1093\/nar\/gkv1370","article-title":"tRNA is a new target for cleavage by a MazF toxin","volume":"44","author":"Schifano","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.pestbp.2024.106261_bb0190","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0032320","article-title":"Removal of hepatitis C virus-infected cells by a zymogenized bacterial toxin","volume":"7","author":"Shapira","year":"2012","journal-title":"PloS One"},{"key":"10.1016\/j.pestbp.2024.106261_bb0195","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1101\/gad.1522007","article-title":"NBK\/BIK antagonizes MCL-1 and BCL-XL and activates BAK-mediated apoptosis in response to protein synthesis inhibition","volume":"21","author":"Shimazu","year":"2007","journal-title":"Genes Dev."},{"key":"10.1016\/j.pestbp.2024.106261_bb0200","first-page":"228","article-title":"Regression of solid tumors by induction of MazF, a bacterial mRNA endoribonuclease","volume":"24","author":"Shimazu","year":"2014","journal-title":"J. Mol. Microbiol. Biotechnol."},{"key":"10.1016\/j.pestbp.2024.106261_bb0205","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.molcel.2013.09.006","article-title":"Structural basis of mRNA recognition and cleavage by toxin MazF and its regulation by antitoxin MazE in Bacillus subtilis","volume":"52","author":"Simanshu","year":"2013","journal-title":"Mol. Cell"},{"issue":"11","key":"10.1016\/j.pestbp.2024.106261_bb0210","doi-asserted-by":"crossref","first-page":"4074","DOI":"10.1073\/pnas.0407475102","article-title":"Development without germ cells: the role of the germ line in zebrafish sex differentiation","volume":"102","author":"Slanchev","year":"2005","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.pestbp.2024.106261_bb0215","doi-asserted-by":"crossref","first-page":"1803","DOI":"10.1007\/s00253-021-11134-z","article-title":"Toxin-antitoxin systems and their medical applications: current status and future perspective","volume":"105","author":"Srivastava","year":"2021","journal-title":"Appl Microbiol Biotechno"},{"key":"10.1016\/j.pestbp.2024.106261_bb0220","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.pep.2015.01.001","article-title":"An efficient method for the purification of proteins from four distinct toxin-antitoxin modules","volume":"108","author":"Sterckx","year":"2015","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.pestbp.2024.106261_bb0225","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1016\/j.jmb.2016.03.007","article-title":"A unique hetero-hexadecameric architecture displayed by the Escherichia coli O157 PaaA2-ParE2 antitoxin-toxin complex","volume":"428","author":"Sterckx","year":"2016","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.pestbp.2024.106261_bb0230","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.ab.2009.07.023","article-title":"Culture of Escherichia coli in SOC medium improves the cloning efficiency of toxic protein genes","volume":"394","author":"Sun","year":"2009","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.pestbp.2024.106261_bb0235","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1078\/1438-4221-00164","article-title":"Molecular interactions of the CcdB poison with its bacterial target, the DNA gyrase","volume":"291","author":"Van Melderen","year":"2002","journal-title":"Int. J. Med. Microbiol."},{"issue":"16","key":"10.1016\/j.pestbp.2024.106261_bb0240","doi-asserted-by":"crossref","first-page":"5577","DOI":"10.1128\/AEM.05068-11","article-title":"Toxin-antitoxin systems influence biofilm and persister cell formation and the general stress response","volume":"77","author":"Wang","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.pestbp.2024.106261_bb0245","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1016\/j.tim.2020.12.001","article-title":"Type VII toxin\/antitoxin classification system for antitoxins that enzymatically neutralize toxins","volume":"29","author":"Wang","year":"2021","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.pestbp.2024.106261_bb0250","doi-asserted-by":"crossref","first-page":"D749","DOI":"10.1093\/nar\/gkx1033","article-title":"TADB 2.0: an updated database of bacterial type II toxin-antitoxin loci","volume":"46","author":"Xie","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.pestbp.2024.106261_bb0255","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1038\/nrmicro2651","article-title":"Regulation of growth and death in Escherichia coli by toxin-antitoxin systems","volume":"9","author":"Yamaguchi","year":"2011","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.pestbp.2024.106261_bb0260","first-page":"10387","article-title":"An endogenous protein inhibitor, YjhX (TopAI), for topoisomerase I from Escherichia coli","volume":"43","author":"Yamaguchi","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.pestbp.2024.106261_bb0265","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1038\/ncomms1621","article-title":"Inhibition of specific gene expressions by protein-mediated mRNA interference","volume":"3","author":"Yamaguchi","year":"2012","journal-title":"Nat. Commun."},{"key":"10.1016\/j.pestbp.2024.106261_bb0270","doi-asserted-by":"crossref","first-page":"3143","DOI":"10.1074\/jbc.M411811200","article-title":"Insights into the mRNA cleavage mechanism by MazF, an mRNA interferase","volume":"280","author":"Zhang","year":"2005","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.pestbp.2024.106261_bb0275","doi-asserted-by":"crossref","DOI":"10.1016\/j.pestbp.2021.104814","article-title":"Functional characterization of kid-Kis and MazF-MazE in Sf9 cells and Mythimna separata embryos","volume":"174","author":"Zhang","year":"2021","journal-title":"Pestic. Biochem. Physiol."}],"container-title":["Pesticide Biochemistry and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0048357524004942?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0048357524004942?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T20:15:31Z","timestamp":1740514531000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0048357524004942"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3]]},"references-count":55,"alternative-id":["S0048357524004942"],"URL":"https:\/\/doi.org\/10.1016\/j.pestbp.2024.106261","relation":{},"ISSN":["0048-3575"],"issn-type":[{"type":"print","value":"0048-3575"}],"subject":[],"published":{"date-parts":[[2025,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A streamlined cloning and toxicity evaluation strategy of toxin-antitoxin systems in insect cells","name":"articletitle","label":"Article Title"},{"value":"Pesticide Biochemistry and Physiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.pestbp.2024.106261","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"106261"}}