{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T21:03:49Z","timestamp":1790629429324,"version":"4.1.0"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100012165","name":"Key Technologies Research and Development Program","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012165","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Pesticide Biochemistry and Physiology"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.pestbp.2026.107282","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T15:37:05Z","timestamp":1785253025000},"page":"107282","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["CRISPR\/Cas9-mediated knockout of CsRDL1 decreases the susceptibility of Chilo suppressalis to cyproflanilide"],"prefix":"10.1016","volume":"223","author":[{"given":"Jungang","family":"Zhou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziren","family":"Zang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongshuai","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinjun","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiao","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hualiang","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbing","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youzhi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.pestbp.2026.107282_bb0005","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.pestbp.2018.02.002","article-title":"Fluxametamide: a novel isoxazoline insecticide that acts via distinctive antagonism of insect ligand-gated chloride channels","volume":"151","author":"Asahi","year":"2018","journal-title":"Pestic. Biochem. Physiol."},{"issue":"10","key":"10.1016\/j.pestbp.2026.107282_bb0010","doi-asserted-by":"crossref","first-page":"4078","DOI":"10.1002\/ps.7606","article-title":"External amino acid residues of insect GABA receptor channels dictate the action of the isoxazoline ectoparasiticide fluralaner","volume":"79","author":"Asahi","year":"2023","journal-title":"Pest Manag. Sci."},{"issue":"2","key":"10.1016\/j.pestbp.2026.107282_bb0015","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.ymeth.2014.02.019","article-title":"CRISPR\/Cas9 mediated genome engineering in Drosophila","volume":"69","author":"Bassett","year":"2014","journal-title":"Methods"},{"issue":"1","key":"10.1016\/j.pestbp.2026.107282_bb0020","doi-asserted-by":"crossref","first-page":"2518","DOI":"10.1038\/s41467-022-30165-2","article-title":"WAKE-mediated modulation of cVA perception via a hierarchical neuro-endocrine axis in Drosophila male-male courtship behaviour","volume":"13","author":"Chen","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.pestbp.2026.107282_bb0025","article-title":"Comparative susceptibility of Aphis gossypii glover (Hemiptera: Aphididae) on cotton crops to imidacloprid and a novel insecticide cyproflanilide in China","volume":"192","author":"Cheng","year":"2022","journal-title":"Ind. Crop. Prod."},{"issue":"3","key":"10.1016\/j.pestbp.2026.107282_bb0030","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0175177","article-title":"GABAA receptor-expressing neurons promote consumption in Drosophila melanogaster","volume":"12","author":"Cheung","year":"2017","journal-title":"PloS One"},{"issue":"50","key":"10.1016\/j.pestbp.2026.107282_bb0035","doi-asserted-by":"crossref","first-page":"14768","DOI":"10.1021\/acs.jafc.0c05728","article-title":"Exploring the interaction mechanism of desmethyl-broflanilide in insect GABA receptors and screening potential antagonists by in silico simulations","volume":"68","author":"Gao","year":"2020","journal-title":"J. Agric. Food Chem."},{"issue":"11","key":"10.1016\/j.pestbp.2026.107282_bb0040","doi-asserted-by":"crossref","first-page":"4342","DOI":"10.1016\/j.jia.2025.03.028","article-title":"Baseline establishment, susceptibility monitoring and risk assessment of cyproflanilide, a novel meta-diamide insecticide, against Chilo suppressalis (Lepidoptera: Crambidae) in China","volume":"24","author":"Ge","year":"2025","journal-title":"J. Integr. Agric."},{"issue":"Pt 1","key":"10.1016\/j.pestbp.2026.107282_bb0045","article-title":"Cyproflanilide, a novel meta-diamide insecticide, overcomes multiple insecticide resistance in Cnaphalocrocis medinalis: evidence from comparative toxicity assays and genome-edited Drosophila","volume":"216","author":"Ge","year":"2026","journal-title":"Pestic. Biochem. Physiol."},{"key":"10.1016\/j.pestbp.2026.107282_bb0050","doi-asserted-by":"crossref","DOI":"10.1016\/j.pestbp.2025.106383","article-title":"CYP321F3 mediates metabolic resistance to methoxyfenozide in rice stem borer, Chilo suppressalis","volume":"210","author":"Guo","year":"2025","journal-title":"Pestic. Biochem. Physiol."},{"issue":"9","key":"10.1016\/j.pestbp.2026.107282_bb0055","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.1038\/s41559-023-02127-4","article-title":"Convergent resistance to GABA receptor neurotoxins through plant\u2013insect coevolution","volume":"7","author":"Guo","year":"2023","journal-title":"Nat. Ecol. Evol."},{"issue":"39","key":"10.1016\/j.pestbp.2026.107282_bb0060","doi-asserted-by":"crossref","first-page":"11582","DOI":"10.1021\/acs.jafc.1c00385","article-title":"Functional characteristics of the lepidopteran ionotropic GABA receptor 8916 subunit interacting with the LCCH3 or the RDL subunit","volume":"69","author":"Huang","year":"2021","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.pestbp.2026.107282_bb0065","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.pestbp.2019.01.001","article-title":"Identification of the ionotropic GABA receptor-like subunits from the striped stem borer, Chilo suppressalis Walker (Lepidoptera: Pyralidae)","volume":"155","author":"Jia","year":"2019","journal-title":"Pestic. Biochem. Physiol."},{"issue":"31","key":"10.1016\/j.pestbp.2026.107282_bb0070","doi-asserted-by":"crossref","first-page":"11875","DOI":"10.1021\/acs.jafc.3c02811","article-title":"Physiological function of RDL1 and RDL2 subunits of the ionotropic GABA receptor in the Spodoptera litura with the CRISPR\/Cas9 system in vivo","volume":"71","author":"Jia","year":"2023","journal-title":"J. Agric. Food Chem."},{"issue":"6","key":"10.1016\/j.pestbp.2026.107282_bb0075","doi-asserted-by":"crossref","first-page":"1721","DOI":"10.1111\/1744-7917.12892","article-title":"Distinct roles of two RDL GABA receptors in fipronil action in the diamondback moth (Plutella xylostella)","volume":"28","author":"Li","year":"2021","journal-title":"Insect. Sci."},{"issue":"1","key":"10.1016\/j.pestbp.2026.107282_bb0080","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1127\/entomologia\/2024\/2807","article-title":"Csu-miR-375 and Csu-miR-11631 regulate ryanodine receptor to induce chlorantraniliprole resistance in Chilo suppressalis","volume":"45","author":"Li","year":"2025","journal-title":"Entomol. Gen."},{"key":"10.1016\/j.pestbp.2026.107282_bb0085","doi-asserted-by":"crossref","first-page":"2963","DOI":"10.6023\/cjoc202003025","article-title":"Synthesis and insecticidal activity of novel meta-diamide compounds containing cyclopropyl group","volume":"40","author":"Luo","year":"2020","journal-title":"Chin. J. Org. Chem."},{"issue":"49","key":"10.1016\/j.pestbp.2026.107282_bb0090","doi-asserted-by":"crossref","first-page":"19012","DOI":"10.1074\/jbc.RA118.005365","article-title":"Multiple combinations of RDL subunits diversify the repertoire of GABA receptors in the honey bee parasite Varroa destructor","volume":"293","author":"M\u00e9nard","year":"2018","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.pestbp.2026.107282_bb0095","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.pestbp.2018.11.017","article-title":"Knockdown of the GABA receptor RDL genes decreases abamectin susceptibility in the rice stem borer, Chilo suppressalis","volume":"153","author":"Meng","year":"2019","journal-title":"Pestic. Biochem. Physiol."},{"issue":"5","key":"10.1016\/j.pestbp.2026.107282_bb0100","doi-asserted-by":"crossref","first-page":"724","DOI":"10.3390\/plants14050724","article-title":"Dynamic monitoring of Chilo suppressalis resistance to insecticides and the potential influencing factors","volume":"14","author":"Mo","year":"2025","journal-title":"Plants"},{"issue":"3\u20134","key":"10.1016\/j.pestbp.2026.107282_bb0105","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1584\/jpestics.D13-024","article-title":"A comparison of the modes of action of novel meta-diamide insecticides and conventional noncompetitive antagonists on the Spodoptera litura RDL GABA receptor","volume":"38","author":"Nakao","year":"2013","journal-title":"J. Pestic. Sci."},{"issue":"5","key":"10.1016\/j.pestbp.2026.107282_bb0110","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1124\/mol.117.109413","article-title":"A single amino acid substitution in the third transmembrane region has opposite impacts on the selectivity of the parasiticides fluralaner and ivermectin for ligand-gated chloride channels","volume":"92","author":"Nakata","year":"2017","journal-title":"Mol. Pharmacol."},{"key":"10.1016\/j.pestbp.2026.107282_bb0115","doi-asserted-by":"crossref","DOI":"10.1016\/j.pestbp.2024.105776","article-title":"Knock-in mutagenesis in Drosophila RDL underscores the critical role of the conserved M3 glycine in mediating the actions of broflanilide and isocycloseram on GABA receptors","volume":"199","author":"Ozoe","year":"2024","journal-title":"Pestic. Biochem. Physiol."},{"key":"10.1016\/j.pestbp.2026.107282_bb0120","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.ibmb.2018.01.003","article-title":"Molecular cloning, spatiotemporal and functional expression of GABA receptor subunits RDL1 and RDL2 of the rice stem borer Chilo suppressalis","volume":"94","author":"Sheng","year":"2018","journal-title":"Insect Biochem. Mol. Biol."},{"issue":"1","key":"10.1016\/j.pestbp.2026.107282_bb0125","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/s12864-022-09095-2","article-title":"The transcriptomic profile of Spodoptera frugiperda differs in response to a novel insecticide, cyproflanilide, compared to chlorantraniliprole and avermectin","volume":"24","author":"Shu","year":"2023","journal-title":"BMC Genomics"},{"issue":"2","key":"10.1016\/j.pestbp.2026.107282_bb0130","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1002\/ps.7252","article-title":"CRISPR\/Cas9-mediated D472N substitution in the RDL1 of Plutella xylostella confers low resistance to abamectin","volume":"79","author":"Sun","year":"2023","journal-title":"Pest Manag. Sci."},{"issue":"12","key":"10.1016\/j.pestbp.2026.107282_bb0135","doi-asserted-by":"crossref","first-page":"2272","DOI":"10.1111\/pbi.13906","article-title":"FAR knockout significantly inhibits Chilo suppressalis survival and transgene expression of double-stranded FAR in rice exhibits strong pest resistance","volume":"20","author":"Sun","year":"2022","journal-title":"Plant Biotechnol. J."},{"issue":"7","key":"10.1016\/j.pestbp.2026.107282_bb0140","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: molecular evolutionary genetics analysis version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.pestbp.2026.107282_bb0145","doi-asserted-by":"crossref","first-page":"4116","DOI":"10.3390\/molecules24224116","article-title":"Identification of the fipronil resistance associated mutations in Nilaparvata lugens GABA receptors by molecular modeling","volume":"24","author":"Tian","year":"2019","journal-title":"Molecules"},{"issue":"16","key":"10.1016\/j.pestbp.2026.107282_bb0150","doi-asserted-by":"crossref","first-page":"9920","DOI":"10.1021\/acs.jafc.5c00470","article-title":"Y4667d mutation in the ryanodine receptor confers high level resistance to diamide insecticides in the rice stem borer, Chilo suppressalis Walker (Lepidoptera: Crambidae)","volume":"73","author":"Wang","year":"2025","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.pestbp.2026.107282_bb0155","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.pestbp.2016.08.007","article-title":"Molecular characterization and expression pattern of three GABA receptor-like subunits in the small brown planthopper Laodelphax striatellus (Hemiptera: Delphacidae)","volume":"136","author":"Wei","year":"2017","journal-title":"Pestic. Biochem. Physiol."},{"key":"10.1016\/j.pestbp.2026.107282_bb0160","doi-asserted-by":"crossref","DOI":"10.1016\/j.ibmb.2025.104327","article-title":"A CRISPR\/Cas9-induced point mutation on the GABA receptor subunit RDL confers high resistance to phenylpyrazole insecticides in the rice planthopper Laodelphax striatellus","volume":"181","author":"Wu","year":"2025","journal-title":"Insect Biochem. Mol. Biol."},{"issue":"W1","key":"10.1016\/j.pestbp.2026.107282_bb0165","doi-asserted-by":"crossref","first-page":"W587","DOI":"10.1093\/nar\/gkad359","article-title":"Tree visualization by one table (tvBOT): a web application for visualizing, modifying and annotating phylogenetic trees","volume":"51","author":"Xie","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.pestbp.2026.107282_bb0170","doi-asserted-by":"crossref","DOI":"10.1016\/j.pestbp.2024.105972","article-title":"GABA-gated chloride channel mutation (RDL) induces cholinergic physiological compensation resulting in cross resistance in Drosophila melanogaster","author":"Xie","year":"2024","journal-title":"Pestic. Biochem. Physiol."},{"issue":"W1","key":"10.1016\/j.pestbp.2026.107282_bb0175","doi-asserted-by":"crossref","first-page":"W420","DOI":"10.1093\/nar\/gkac373","article-title":"IBS 2.0: an upgraded illustrator for the visualization of biological sequences","volume":"50","author":"Xie","year":"2022","journal-title":"Nucleic Acids Res."},{"issue":"3","key":"10.1016\/j.pestbp.2026.107282_bb0180","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1002\/ps.5171","article-title":"Constitutive overexpression of cytochrome P450 monooxygenase genes contributes to chlorantraniliprole resistance in Chilo suppressalis (Walker)","volume":"75","author":"Xu","year":"2019","journal-title":"Pest. Manag. Sci."},{"key":"10.1016\/j.pestbp.2026.107282_bb0185","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.pestbp.2019.11.001","article-title":"Effects of intersubunit amino acid substitutions on GABA receptor sensitivity to the ectoparasiticide fluralaner","volume":"163","author":"Yamato","year":"2020","journal-title":"Pestic. Biochem. Physiol."},{"key":"10.1016\/j.pestbp.2026.107282_bb0190","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2021.147088","article-title":"Floating chitosan-alginate microspheres loaded with chlorantraniliprole effectively control Chilo suppressalis (Walker) and Sesamia inferens (Walker) in rice fields","volume":"783","author":"Yang","year":"2021","journal-title":"Sci. Total Environ."},{"issue":"Pt 2","key":"10.1016\/j.pestbp.2026.107282_bb0195","article-title":"Ryanodine receptor I4734M mutation confers diamide resistance but reduces invasiveness in Spodoptera frugiperda","volume":"331","author":"Yang","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"issue":"10","key":"10.1016\/j.pestbp.2026.107282_bb0200","doi-asserted-by":"crossref","DOI":"10.3390\/agronomy14102249","article-title":"Resistance realized heritability and fitness cost of cyproflanilide in rice stem borer, Chilo suppressalis (Lepidoptera: Pyralidae)","volume":"14","author":"Zhang","year":"2024","journal-title":"Agronomy"},{"issue":"6","key":"10.1016\/j.pestbp.2026.107282_bb0205","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1010814","article-title":"G3\u2032MTMD3 in the insect GABA receptor subunit, RDL, confers resistance to broflanilide and fluralaner","volume":"19","author":"Zhang","year":"2023","journal-title":"PLoS Genet."},{"issue":"41","key":"10.1016\/j.pestbp.2026.107282_bb0210","first-page":"22554","article-title":"N318L blocks the interaction of fluralaner but not broflanilide or fipronil with the insect GABA receptor in vivo","volume":"72","author":"Zhang","year":"2024","journal-title":"J. Agric. Food Chem."},{"issue":"6","key":"10.1016\/j.pestbp.2026.107282_bb0215","doi-asserted-by":"crossref","first-page":"5461","DOI":"10.3390\/ijms24065461","article-title":"Transcriptomic analysis reveals the detoxification mechanism of Chilo suppressalis in response to the novel pesticide cyproflanilide","volume":"24","author":"Zhou","year":"2023","journal-title":"Int. J. Mol. Sci."},{"issue":"5","key":"10.1016\/j.pestbp.2026.107282_bb0220","doi-asserted-by":"crossref","first-page":"334","DOI":"10.3390\/insects15050334","article-title":"Effects of RDL GABA receptor point mutants on susceptibility to meta-diamide and isoxazoline insecticides in Drosophila melanogaster","volume":"15","author":"Zhou","year":"2024","journal-title":"Insects"},{"key":"10.1016\/j.pestbp.2026.107282_bb0225","doi-asserted-by":"crossref","DOI":"10.1016\/j.ibmb.2025.104356","article-title":"Doublesex knockout via CRISPR\/Cas9 disrupts fertility and sexual dimorphism of wings in the rice stem borer, Chilo suppressalis","volume":"182","author":"Zhu","year":"2025","journal-title":"Insect Biochem. Mol. Biol."}],"container-title":["Pesticide Biochemistry and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0048357526003433?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0048357526003433?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T20:28:29Z","timestamp":1790627309000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0048357526003433"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":45,"alternative-id":["S0048357526003433"],"URL":"https:\/\/doi.org\/10.1016\/j.pestbp.2026.107282","relation":{},"ISSN":["0048-3575"],"issn-type":[{"value":"0048-3575","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"CRISPR\/Cas9-mediated knockout of CsRDL1 decreases the susceptibility of Chilo suppressalis to cyproflanilide","name":"articletitle","label":"Article Title"},{"value":"Pesticide Biochemistry and Physiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.pestbp.2026.107282","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"107282"}}