{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:33:13Z","timestamp":1791408793499,"version":"4.3.3"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundations of China","doi-asserted-by":"crossref","award":["81473215"],"award-info":[{"award-number":["81473215"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Special Foundation for Guangdong Provincial Public Welfare Research and Capacity Building","award":["2014A020212290"],"award-info":[{"award-number":["2014A020212290"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Pharmacological Research"],"published-print":{"date-parts":[[2019,1]]},"DOI":"10.1016\/j.phrs.2018.11.017","type":"journal-article","created":{"date-parts":[[2018,11,14]],"date-time":"2018-11-14T12:10:54Z","timestamp":1542197454000},"page":"153-157","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":41,"special_numbering":"C","title":["How CaV1.2-bound verapamil blocks Ca2+ influx into cardiomyocyte: Atomic level views"],"prefix":"10.1016","volume":"139","author":[{"given":"Wei","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ganggang","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.phrs.2018.11.017_bib0005","series-title":"Heart Physiology and Pathophysiology","first-page":"949","article-title":"Calcium overload in ischemia\/reperfusion injury","author":"Dhalla","year":"2001"},{"issue":"11","key":"10.1016\/j.phrs.2018.11.017_bib0010","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1056\/NEJMra071667","article-title":"Myocardial reperfusion injury","volume":"357","author":"Yellon","year":"2007","journal-title":"N. Engl. J. Med."},{"issue":"9","key":"10.1016\/j.phrs.2018.11.017_bib0015","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1111\/j.1751-7176.2011.00513.x","article-title":"Calcium channel blockers","volume":"13","author":"Elliott","year":"2011","journal-title":"J. Clin. Hypertens."},{"issue":"4\u20135","key":"10.1016\/j.phrs.2018.11.017_bib0020","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1159\/000016320","article-title":"Pharmacology, structure and function of cardiac L-type Ca2+ channels","volume":"9","author":"Striessnig","year":"1999","journal-title":"Cell. Physiol. Biochem."},{"issue":"6868","key":"10.1016\/j.phrs.2018.11.017_bib0025","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1038\/415198a","article-title":"Cardiac excitation-contraction coupling","volume":"415","author":"Bers","year":"2002","journal-title":"Nature"},{"issue":"8","key":"10.1016\/j.phrs.2018.11.017_bib0030","doi-asserted-by":"crossref","first-page":"2134","DOI":"10.1016\/j.celrep.2017.10.105","article-title":"L-type voltage-gated Ca2+ channels regulate synaptic activity-triggered recycling endosome fusion in neuronal dendrites","volume":"21","author":"Hiester","year":"2017","journal-title":"Cell Rep."},{"issue":"4","key":"10.1016\/j.phrs.2018.11.017_bib0035","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1124\/pr.57.4.5","article-title":"International Union of Pharmacology. XLVIII. Nomenclature and structure-function relationships of voltage-gated calcium channels","volume":"57","author":"Catterall","year":"2005","journal-title":"Pharmacol. Rev."},{"key":"10.1016\/j.phrs.2018.11.017_bib0040","doi-asserted-by":"crossref","unstructured":"H. Glossmann, J. Striessnig, Molecular properties of calcium channels, Reviews of Physiology, Biochemistry and Pharmacology, Springer-Verlag, pp. 1\u2013105. https:\/\/doi.org\/10.1007\/bfb0031018.","DOI":"10.1007\/BFb0031018"},{"issue":"12","key":"10.1016\/j.phrs.2018.11.017_bib0045","doi-asserted-by":"crossref","first-page":"3306","DOI":"10.1172\/JCI27167","article-title":"The L-type calcium channel in the heart: the beat goes on","volume":"115","author":"Bodi","year":"2005","journal-title":"J. Clin. Invest."},{"issue":"May","key":"10.1016\/j.phrs.2018.11.017_bib0050","article-title":"Discovery and development of calcium channel blockers","volume":"8","author":"Godfraind","year":"2017","journal-title":"Front. Pharmacol."},{"issue":"6230","key":"10.1016\/j.phrs.2018.11.017_bib0055","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1038\/340230a0","article-title":"Primary structure and functional expression of the cardiac dihydropyridine-sensitive calcium channel","volume":"340","author":"Mikami","year":"1989","journal-title":"Nature"},{"issue":"1","key":"10.1016\/j.phrs.2018.11.017_bib0060","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1146\/annurev.pharmtox.37.1.361","article-title":"Molecular determinants of drug binding and action on L-type calcium channels","volume":"37","author":"Hockerman","year":"1997","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"issue":"14","key":"10.1016\/j.phrs.2018.11.017_bib0065","doi-asserted-by":"crossref","first-page":"9409","DOI":"10.1074\/jbc.274.14.9409","article-title":"A region in IVS5 of the human cardiac L-type calcium channel is required for the use-dependent block by phenylalkylamines and benzothiazepines","volume":"274","author":"Motoike","year":"1999","journal-title":"J. Biol. Chem."},{"issue":"6","key":"10.1016\/j.phrs.2018.11.017_bib0070","doi-asserted-by":"crossref","first-page":"1264","DOI":"10.1124\/mol.58.6.1264","article-title":"Molecular determinants of diltiazem block in domains IIIS6 and IVS6 of L-type Ca2+ channels","volume":"58","author":"Hockerman","year":"2000","journal-title":"Mol. Pharmacol."},{"issue":"3","key":"10.1016\/j.phrs.2018.11.017_bib0075","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1073\/pnas.80.3.860","article-title":"A unitary mechanism of calcium antagonist drug action","volume":"80","author":"Murphy","year":"1983","journal-title":"Proc. Natl. Acad. Sci."},{"issue":"3","key":"10.1016\/j.phrs.2018.11.017_bib0080","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1124\/mol.110.069492","article-title":"Verapamil block of T-type calcium channels","volume":"79","author":"Bergson","year":"2010","journal-title":"Mol. Pharmacol."},{"issue":"38","key":"10.1016\/j.phrs.2018.11.017_bib0085","doi-asserted-by":"crossref","first-page":"22119","DOI":"10.1074\/jbc.270.38.22119","article-title":"Molecular determinants of high affinity phenylalkylamine block of L-type calcium channels","volume":"270","author":"Hockerman","year":"1995","journal-title":"J. Biol. Chem."},{"issue":"30","key":"10.1016\/j.phrs.2018.11.017_bib0090","doi-asserted-by":"crossref","first-page":"18759","DOI":"10.1074\/jbc.272.30.18759","article-title":"Molecular determinants of high affinity phenylalkylamine block of L-type calcium channels in transmembrane segment IIIS6 and the pore region of the \u03b11 subunit","volume":"272","author":"Hockerman","year":"1997","journal-title":"J. Biol. Chem."},{"issue":"3","key":"10.1016\/j.phrs.2018.11.017_bib0095","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/S0959-4388(03)00066-7","article-title":"Auxiliary subunits: essential components of the voltage-gated calcium channel complex","volume":"13","author":"Arikkath","year":"2003","journal-title":"Curr. Opin. Neurobiol."},{"issue":"7619","key":"10.1016\/j.phrs.2018.11.017_bib0100","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1038\/nature19321","article-title":"Structure of the voltage-gated calcium channel CaV1.1 at 3.6\u202f\u00c5 resolution","volume":"537","author":"Wu","year":"2016","journal-title":"Nature"},{"issue":"8","key":"10.1016\/j.phrs.2018.11.017_bib0105","doi-asserted-by":"crossref","first-page":"7159","DOI":"10.1074\/jbc.M308057200","article-title":"The three-dimensional structure of the cardiac L-type voltage-gated calcium channel","volume":"279","author":"Wang","year":"2003","journal-title":"J. Biol. Chem."},{"issue":"1","key":"10.1016\/j.phrs.2018.11.017_bib0110","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/nprot.2008.197","article-title":"Protein structure homology modeling using SWISS-MODEL workspace","volume":"4","author":"Bordoli","year":"2008","journal-title":"Nat. Protoc."},{"issue":"2","key":"10.1016\/j.phrs.2018.11.017_bib0115","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1093\/bioinformatics\/bti770","article-title":"The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling","volume":"22","author":"Arnold","year":"2005","journal-title":"Bioinformatics"},{"issue":"5","key":"10.1016\/j.phrs.2018.11.017_bib0120","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1016\/0896-6273(95)90100-0","article-title":"Ca2+ channel selectivity at a single locus for high-affinity Ca2+ interactions","volume":"15","author":"Ellinor","year":"1995","journal-title":"Neuron"},{"issue":"3","key":"10.1016\/j.phrs.2018.11.017_bib0125","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1085\/jgp.116.3.349","article-title":"The EEEE locus is the sole high-affinity Ca2+ binding structure in the pore of a voltage-gated Ca2+ channel","volume":"116","author":"Cibulsky","year":"2000","journal-title":"J. Gen. Physiol."},{"issue":"41","key":"10.1016\/j.phrs.2018.11.017_bib0130","doi-asserted-by":"crossref","first-page":"31778","DOI":"10.1074\/jbc.M004829200","article-title":"Side chain orientation in the selectivity filter of a voltage-gated Ca2+ channel","volume":"275","author":"Wu","year":"2000","journal-title":"J. Biol. Chem."},{"issue":"7481","key":"10.1016\/j.phrs.2018.11.017_bib0135","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1038\/nature12775","article-title":"Structural basis for Ca2+ selectivity of a voltage-gated calcium channel","volume":"505","author":"Tang","year":"2013","journal-title":"Nature"},{"issue":"W1","key":"10.1016\/j.phrs.2018.11.017_bib0140","doi-asserted-by":"crossref","first-page":"W252","DOI":"10.1093\/nar\/gku340","article-title":"SWISS-MODEL: modelling protein tertiary and quaternary structure using evolutionary information","volume":"42","author":"Biasini","year":"2014","journal-title":"Nucleic Acids Res."},{"issue":"1","key":"10.1016\/j.phrs.2018.11.017_bib0145","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1113\/jphysiol.1984.sp015352","article-title":"Non-selective conductance in calcium channels of frog muscle: calcium selectivity in a single-file pore","volume":"353","author":"Almers","year":"1984","journal-title":"J. Physiol."},{"issue":"5986","key":"10.1016\/j.phrs.2018.11.017_bib0150","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1038\/311538a0","article-title":"Different modes of Ca channel gating behaviour favoured by dihydropyridine Ca agonists and antagonists","volume":"311","author":"Hess","year":"1984","journal-title":"Nature"},{"issue":"20","key":"10.1016\/j.phrs.2018.11.017_bib0155","doi-asserted-by":"crossref","first-page":"7149","DOI":"10.1073\/pnas.82.20.7149","article-title":"The Ca channel in skeletal muscle is a large pore","volume":"82","author":"McCleskey","year":"1985","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.phrs.2018.11.017_bib0160","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1021\/ct100578z","article-title":"PROPKA3: consistent treatment of internal and surface residues in empirical pKa predictions","volume":"7","author":"Olsson","year":"2011","journal-title":"J. Chem. Theory Comput."},{"issue":"12","key":"10.1016\/j.phrs.2018.11.017_bib0165","doi-asserted-by":"crossref","first-page":"127002","DOI":"10.1088\/1361-6501\/aa8c1c","article-title":"Gravity-driven pH adjustment for site-specific protein pKa measurement by solution-state NMR","volume":"28","author":"Li","year":"2017","journal-title":"Meas. Sci. Technol."},{"issue":"4","key":"10.1016\/j.phrs.2018.11.017_bib0170","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1023\/A:1021933504909","article-title":"Molecular basis of drug interaction with L-type Ca2+ channels","volume":"30","author":"Mitterdorfer","year":"1998","journal-title":"J. Bioenerg. Biomembr."},{"issue":"6","key":"10.1016\/j.phrs.2018.11.017_bib0175","doi-asserted-by":"crossref","first-page":"482","DOI":"10.4161\/chan.5.6.18354","article-title":"A molecular model of the inner pore of the Ca2+ channel in its open state","volume":"5","author":"Lipkind","year":"2011","journal-title":"Channels"},{"issue":"7618","key":"10.1016\/j.phrs.2018.11.017_bib0180","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1038\/nature19102","article-title":"Structural basis for inhibition of a voltage-gated Ca2+ channel by Ca2+ antagonist drugs","volume":"537","author":"Tang","year":"2016","journal-title":"Nature"},{"issue":"6","key":"10.1016\/j.phrs.2018.11.017_bib0185","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1007\/BF00166901","article-title":"Action of tertiary phenylalkylamines on cardiac transient outward current from outside the cell membrane","volume":"354","author":"Wegener","year":"1996","journal-title":"Naunyn-Schmiedeberg's Arch. Pharmacol."},{"issue":"2","key":"10.1016\/j.phrs.2018.11.017_bib0190","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1007\/BF00517988","article-title":"Investigations on the structure-activity relationships of verapamil","volume":"302","author":"Mannhold","year":"1978","journal-title":"Naunyn-Schmiedeberg's Arch. Pharmacol."},{"issue":"4","key":"10.1016\/j.phrs.2018.11.017_bib0195","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1007\/BF00581411","article-title":"Does the organic calcium channel blocker D600 act from inside or outside on the cardiac cell membrane?","volume":"393","author":"Hescheler","year":"1982","journal-title":"Pfl\u00fcg. Arch. Eur. J. Physiol."},{"issue":"4","key":"10.1016\/j.phrs.2018.11.017_bib0200","doi-asserted-by":"crossref","first-page":"C689","DOI":"10.1152\/ajpcell.1989.257.4.C689","article-title":"D600 block of L-type Ca2+ channel in vascular smooth muscle cells: comparison with permanently charged derivative, D890","volume":"257","author":"Leblanc","year":"1989","journal-title":"Am. J. Physiol. Cell Physiol."},{"issue":"1","key":"10.1016\/j.phrs.2018.11.017_bib0205","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/0896-6273(91)90073-9","article-title":"Distinct calcium channels are generated by alternative splicing and are differentially expressed in the mammalian CNS","volume":"7","author":"Snutch","year":"1991","journal-title":"Neuron"},{"issue":"40","key":"10.1016\/j.phrs.2018.11.017_bib0210","doi-asserted-by":"crossref","first-page":"24471","DOI":"10.1074\/jbc.271.40.24471","article-title":"Transfer of high sensitivity for benzothiazepines from L-type to class A (BI) calcium channels","volume":"271","author":"Hering","year":"1996","journal-title":"J. Biol. Chem."},{"issue":"2","key":"10.1016\/j.phrs.2018.11.017_bib0215","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1111\/j.1469-7793.2000.t01-1-00237.x","article-title":"Molecular determinants of inactivation in voltage-gated Ca2+ channels","volume":"528","author":"Hering","year":"2000","journal-title":"J. Physiol."},{"key":"10.1016\/j.phrs.2018.11.017_bib0220","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/msb.2011.75","article-title":"Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega","volume":"7","author":"Sievers","year":"2011","journal-title":"Mol. Syst. Biol."},{"issue":"Web Server","key":"10.1016\/j.phrs.2018.11.017_bib0225","doi-asserted-by":"crossref","first-page":"W363","DOI":"10.1093\/nar\/gki481","article-title":"PatchDock and SymmDock: servers for rigid and symmetric docking","volume":"33","author":"Schneidman-Duhovny","year":"2005","journal-title":"Nucleic Acids Res."},{"issue":"4","key":"10.1016\/j.phrs.2018.11.017_bib0230","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1107\/S0108270185004875","article-title":"Structure of \u03b1-isopropyl-\u03b1-[(n-methyl-n-homoveratryl)-\u03b3-aminopropyl]-3, 4-dimethoxyphenylacetonitrile hydrochloride, verapamil, C27H38N2O4.HCl","volume":"41","author":"Carpy","year":"1985","journal-title":"Acta Crystallogr. C: Crystal Struct. Commun."},{"issue":"3","key":"10.1016\/j.phrs.2018.11.017_bib0235","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/j.cell.2016.12.030","article-title":"Structural titration of Slo2.2, a Na+-dependent K+ channel","volume":"168","author":"Hite","year":"2017","journal-title":"Cell"},{"issue":"1","key":"10.1016\/j.phrs.2018.11.017_bib0240","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1172\/JCI62874","article-title":"Myocardial ischemia-reperfusion injury: a neglected therapeutic target","volume":"123","author":"Hausenloy","year":"2013","journal-title":"J. Clin. Invest."}],"container-title":["Pharmacological Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1043661818307680?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1043661818307680?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T21:55:50Z","timestamp":1760910950000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1043661818307680"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1]]},"references-count":48,"alternative-id":["S1043661818307680"],"URL":"https:\/\/doi.org\/10.1016\/j.phrs.2018.11.017","relation":{},"ISSN":["1043-6618"],"issn-type":[{"value":"1043-6618","type":"print"}],"subject":[],"published":{"date-parts":[[2019,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"How CaV1.2-bound verapamil blocks Ca2+ influx into cardiomyocyte: Atomic level views","name":"articletitle","label":"Article Title"},{"value":"Pharmacological Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.phrs.2018.11.017","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}