{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T08:30:52Z","timestamp":1793694652687,"version":"4.5.1"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Pharmacological Research"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.phrs.2026.109701","type":"journal-article","created":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T15:01:14Z","timestamp":1792508474000},"page":"109701","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Pharmacological TRPM8 blockade reprograms patient-derived intestinal fibroblasts and mitigates experimental fibrosis through modulation of myofibroblast activation"],"prefix":"10.1016","volume":"233","author":[{"given":"Maria Francesca","family":"Nan\u00ec","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Michela","family":"Rinaldi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ida","family":"Cerqua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"De Cicco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Miraglia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ester","family":"Pagano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiorentina","family":"Roviezzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Piazza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Martinelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca Paola","family":"Tropeano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaetano","family":"Luglio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felice","family":"Crocetto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Dell\u2019Agli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raffaele","family":"Capasso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Borrelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Romano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelo A.","family":"Izzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"5","key":"10.1016\/j.phrs.2026.109701_bib1","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1136\/gutjnl-2023-329963","article-title":"Fibrosis in IBD: from pathogenesis to therapeutic targets","volume":"73","author":"Rieder","year":"2024","journal-title":"Gut"},{"issue":"3","key":"10.1016\/j.phrs.2026.109701_bib2","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1038\/s41575-021-00543-0","article-title":"Revisiting fibrosis in inflammatory bowel disease: the gut thickens","volume":"19","author":"D'Alessio","year":"2022","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"issue":"3","key":"10.1016\/j.phrs.2026.109701_bib3","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1038\/s41385-019-0146-4","article-title":"Induction of autophagy in Cx3cr1(+) mononuclear cells limits IL-23\/IL-22 axis-mediated intestinal fibrosis","volume":"12","author":"Mathur","year":"2019","journal-title":"Mucosal Immunol."},{"issue":"2","key":"10.1016\/j.phrs.2026.109701_bib4","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1053\/j.gastro.2016.09.047","article-title":"Mechanisms, management, and treatment of fibrosis in patients with inflammatory bowel diseases","volume":"152","author":"Rieder","year":"2017","journal-title":"Gastroenterology"},{"issue":"1","key":"10.1016\/j.phrs.2026.109701_bib5","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1038\/s41573-021-00268-4","article-title":"Advances in TRP channel drug discovery: from target validation to clinical studies","volume":"21","author":"Koivisto","year":"2022","journal-title":"Nat. Rev. Drug. Discov."},{"key":"10.1016\/j.phrs.2026.109701_bib6","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2020.590261","article-title":"Involvement of neural transient receptor potential channels in peripheral inflammation","volume":"11","author":"Silverman","year":"2020","journal-title":"Front. Immunol."},{"issue":"6","key":"10.1016\/j.phrs.2026.109701_bib7","doi-asserted-by":"crossref","first-page":"1500","DOI":"10.1038\/mi.2016.16","article-title":"Transient receptor potential melastatin 8 ion channel in macrophages modulates colitis through a balance-shift in TNF-alpha and interleukin-10 production","volume":"9","author":"Khalil","year":"2016","journal-title":"Mucosal Immunol."},{"issue":"18","key":"10.1016\/j.phrs.2026.109701_bib8","doi-asserted-by":"crossref","first-page":"7476","DOI":"10.1073\/pnas.1217431110","article-title":"TRPM8 activation attenuates inflammatory responses in mouse models of colitis","volume":"110","author":"Ramachandran","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"issue":"1","key":"10.1016\/j.phrs.2026.109701_bib9","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1038\/s41419-025-07553-9","article-title":"Dietary targeting of TRPM8 rewires macrophage immunometabolism reducing colitis severity","volume":"16","author":"Cicia","year":"2025","journal-title":"Cell. Death Dis."},{"issue":"3","key":"10.1016\/j.phrs.2026.109701_bib10","doi-asserted-by":"crossref","first-page":"F803","DOI":"10.1152\/ajprenal.90269.2008","article-title":"AMTB, a TRPM8 channel blocker: evidence in rats for activity in overactive bladder and painful bladder syndrome","volume":"295","author":"Lashinger","year":"2008","journal-title":"Am. J. Physiol. Ren. Physiol."},{"key":"10.1016\/j.phrs.2026.109701_bib11","doi-asserted-by":"crossref","first-page":"5732","DOI":"10.1038\/ncomms6732","article-title":"TRPA1 is essential for the vascular response to environmental cold exposure","volume":"5","author":"Aubdool","year":"2014","journal-title":"Nat. Commun."},{"issue":"7","key":"10.1016\/j.phrs.2026.109701_bib12","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1038\/nprot.2008.75","article-title":"Neutral red uptake assay for the estimation of cell viability\/cytotoxicity","volume":"3","author":"Repetto","year":"2008","journal-title":"Nat. Protoc."},{"issue":"2","key":"10.1016\/j.phrs.2026.109701_bib13","doi-asserted-by":"crossref","DOI":"10.1093\/ecco-jcc\/jjae132","article-title":"Pharmacological Inhibition of N-Acylethanolamine Acid Amidase (NAAA) Mitigates Intestinal Fibrosis Through Modulation of Macrophage Activity","volume":"19","author":"Nani","year":"2025","journal-title":"J. Crohns Colitis"},{"issue":"6","key":"10.1016\/j.phrs.2026.109701_bib14","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1016\/j.ceca.2007.03.005","article-title":"Characterisation of TRPM8 as a pharmacophore receptor","volume":"42","author":"Bodding","year":"2007","journal-title":"Cell. Calcium"},{"issue":"2","key":"10.1016\/j.phrs.2026.109701_bib15","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1111\/bph.15960","article-title":"TRPM8 indicates poor prognosis in colorectal cancer patients and its pharmacological targeting reduces tumour growth in mice by inhibiting Wnt\/\u03b2\u2010catenin signalling","volume":"180","author":"Pagano","year":"2023","journal-title":"Br. J. Pharmacol."},{"issue":"4","key":"10.1016\/j.phrs.2026.109701_bib16","doi-asserted-by":"crossref","first-page":"1340","DOI":"10.1016\/j.ajpath.2012.02.004","article-title":"Recent developments in myofibroblast biology: paradigms for connective tissue remodeling","volume":"180","author":"Hinz","year":"2012","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.phrs.2026.109701_bib17","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms10204","article-title":"Epigenetic switch drives the conversion of fibroblasts into proinvasive cancer-associated fibroblasts","volume":"6","author":"Albrengues","year":"2015","journal-title":"Nat. Commun."},{"issue":"1","key":"10.1016\/j.phrs.2026.109701_bib18","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1111\/imr.12972","article-title":"Fibroblasts as confederates of the immune system","volume":"302","author":"Correa-Gallegos","year":"2021","journal-title":"Immunol. Rev."},{"issue":"1","key":"10.1016\/j.phrs.2026.109701_bib19","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/s13578-022-00789-4","article-title":"TRPM8 deficiency attenuates liver fibrosis through S100A9-HNF4alpha signaling","volume":"12","author":"Liu","year":"2022","journal-title":"Cell. Biosci."},{"key":"10.1016\/j.phrs.2026.109701_bib20","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1016\/j.freeradbiomed.2025.10.252","article-title":"Genetic deletion of TRPM8 channels restores microvascular function and mitigates chronic kidney disease progression","volume":"241","author":"Huang","year":"2025","journal-title":"Free. Radic. Biol. Med."},{"issue":"31","key":"10.1016\/j.phrs.2026.109701_bib21","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.adp2211","article-title":"Mechanisms of sensory adaptation and inhibition of the cold and menthol receptor TRPM8","volume":"10","author":"Yin","year":"2024","journal-title":"Sci. Adv."},{"issue":"1","key":"10.1016\/j.phrs.2026.109701_bib22","doi-asserted-by":"crossref","first-page":"6304","DOI":"10.1038\/s41467-022-33835-3","article-title":"TRPM8 contributes to sex dimorphism by promoting recovery of normal sensitivity in a mouse model of chronic migraine","volume":"13","author":"Alarcon-Alarcon","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.phrs.2026.109701_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.physbeh.2022.113729","article-title":"Role of TRPM8 in cold avoidance behaviors and brain activation during innocuous and nocuous cold stimuli","volume":"248","author":"Kasuga","year":"2022","journal-title":"Physiol. Behav."},{"key":"10.1016\/j.phrs.2026.109701_bib24","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2024.1484387","article-title":"A functional unbalance of TRPM8 and Kv1 channels underlies orofacial cold allodynia induced by peripheral nerve damage","volume":"15","author":"Pina","year":"2024","journal-title":"Front. Pharmacol."},{"issue":"4","key":"10.1016\/j.phrs.2026.109701_bib25","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.joen.2010.12.017","article-title":"Human dental pulp fibroblasts express the \"cold-sensing\" transient receptor potential channels TRPA1 and TRPM8","volume":"37","author":"El Karim","year":"2011","journal-title":"J. Endod."},{"key":"10.1016\/j.phrs.2026.109701_bib26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ejphar.2014.01.001","article-title":"Involvement of transient receptor potential melastatin-8 (TRPM8) in menthol-induced calcium entry, reactive oxygen species production and cell death in rheumatoid arthritis rat synovial fibroblasts","volume":"725","author":"Zhu","year":"2014","journal-title":"Eur. J. Pharmacol."},{"issue":"4","key":"10.1016\/j.phrs.2026.109701_bib27","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1016\/j.archoralbio.2014.12.014","article-title":"Induction of IL-6 and IL-8 by activation of thermosensitive TRP channels in human PDL cells","volume":"60","author":"Son","year":"2015","journal-title":"Arch. Oral. Biol."},{"issue":"1","key":"10.1016\/j.phrs.2026.109701_bib28","doi-asserted-by":"crossref","DOI":"10.1093\/ejo\/cjae079","article-title":"Inflammation alters the expression and activity of the mechanosensitive ion channels in periodontal ligament cells","volume":"47","author":"Ren","year":"2024","journal-title":"Eur. J. Orthod."},{"issue":"12","key":"10.1016\/j.phrs.2026.109701_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.jlr.2025.100935","article-title":"TRPM8 inhibition reduces the size but increases the number of lipid droplets in mature adipocytes in vitro","volume":"66","author":"Mahapatra","year":"2025","journal-title":"J. Lipid Res."},{"issue":"11","key":"10.1016\/j.phrs.2026.109701_bib30","doi-asserted-by":"crossref","DOI":"10.3390\/molecules29112602","article-title":"Signal transduction of transient receptor potential TRPM8 channels: role of PIP5K, Gq-Proteins, and c-Jun","volume":"29","author":"Thiel","year":"2024","journal-title":"Molecules"},{"issue":"1","key":"10.1016\/j.phrs.2026.109701_bib31","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1038\/s41419-024-06480-5","article-title":"TRPM8 inhibits substance P release from primary sensory neurons via PKA\/GSK-3beta to protect colonic epithelium in colitis","volume":"15","author":"Zhang","year":"2024","journal-title":"Cell. Death Dis."},{"issue":"8","key":"10.1016\/j.phrs.2026.109701_bib32","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1111\/nmo.12368","article-title":"TRPM8 has a key role in experimental colitis-induced visceral hyperalgesia in mice","volume":"26","author":"Hosoya","year":"2014","journal-title":"Neurogastroenterol. Motil."},{"issue":"1","key":"10.1016\/j.phrs.2026.109701_bib33","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1007\/BF00266843","article-title":"Are the polarization colors of picrosirius red-stained collagen determined only by the diameter of the fibers?","volume":"93","author":"Dayan","year":"1989","journal-title":"Histochemistry"},{"issue":"10","key":"10.1016\/j.phrs.2026.109701_bib34","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1369\/0022155414545787","article-title":"Picrosirius red staining: a useful tool to appraise collagen networks in normal and pathological tissues","volume":"62","author":"Lattouf","year":"2014","journal-title":"J. Histochem. Cytochem."},{"issue":"10","key":"10.1016\/j.phrs.2026.109701_bib35","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1369\/00221554211046777","article-title":"Picrosirius red staining: revisiting its application to the qualitative and quantitative assessment of collagen type I and type III in Tendon","volume":"69","author":"Lopez De Padilla","year":"2021","journal-title":"J. Histochem. Cytochem."},{"issue":"12","key":"10.1016\/j.phrs.2026.109701_bib36","first-page":"1491","article-title":"Cellular and molecular mediators of intestinal fibrosis","volume":"11","author":"Lawrance","year":"2017","journal-title":"J. Crohns Colitis"},{"issue":"1","key":"10.1016\/j.phrs.2026.109701_bib37","doi-asserted-by":"crossref","first-page":"L23","DOI":"10.1152\/ajplung.00210.2021","article-title":"Combined control of the fibroblast contractile program by YAP and TAZ","volume":"322","author":"Link","year":"2022","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol."},{"issue":"10","key":"10.1016\/j.phrs.2026.109701_bib38","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.2174\/156652412803833607","article-title":"Fibroblast activation protein in remodeling tissues","volume":"12","author":"Jacob","year":"2012","journal-title":"Curr. Mol. Med."},{"issue":"2","key":"10.1016\/j.phrs.2026.109701_bib39","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1093\/ibd\/izx008","article-title":"Inhibition of fibroblast activation protein restores a balanced extracellular matrix and reduces fibrosis in Crohn's Disease Strictures Ex Vivo","volume":"24","author":"Truffi","year":"2018","journal-title":"Inflamm. Bowel Dis."},{"issue":"3","key":"10.1016\/j.phrs.2026.109701_bib40","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1007\/s10555-020-09909-3","article-title":"The role of fibroblast activation protein in health and malignancy","volume":"39","author":"Fitzgerald","year":"2020","journal-title":"Cancer Metastas-.-. Rev."},{"issue":"4","key":"10.1016\/j.phrs.2026.109701_bib41","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1039\/c2ib00149g","article-title":"The mechanical memory of lung myofibroblasts","volume":"4","author":"Balestrini","year":"2012","journal-title":"Integr. Biol. (Camb. )"},{"issue":"1","key":"10.1016\/j.phrs.2026.109701_bib42","doi-asserted-by":"crossref","first-page":"7076","DOI":"10.1038\/s41598-024-57776-7","article-title":"Ketotifen directly modifies the fibrotic response of human skin fibroblasts","volume":"14","author":"Leong","year":"2024","journal-title":"Sci. Rep."},{"key":"10.1016\/j.phrs.2026.109701_bib43","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/s12931-016-0328-5","article-title":"Pirfenidone and nintedanib modulate properties of fibroblasts and myofibroblasts in idiopathic pulmonary fibrosis","volume":"17","author":"Lehtonen","year":"2016","journal-title":"Respir. Res."}],"container-title":["Pharmacological Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1043661826016166?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1043661826016166?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T08:18:06Z","timestamp":1793693886000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1043661826016166"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":43,"alternative-id":["S1043661826016166"],"URL":"https:\/\/doi.org\/10.1016\/j.phrs.2026.109701","relation":{},"ISSN":["1043-6618"],"issn-type":[{"value":"1043-6618","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Pharmacological TRPM8 blockade reprograms patient-derived intestinal fibroblasts and mitigates experimental fibrosis through modulation of myofibroblast activation","name":"articletitle","label":"Article Title"},{"value":"Pharmacological Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.phrs.2026.109701","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"109701"}}