{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T00:08:21Z","timestamp":1792886901266,"version":"4.5.0"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,1,23]],"date-time":"2025-01-23T00:00:00Z","timestamp":1737590400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018571","name":"Specific Research Project of Guangxi for Research Bases and Talents","doi-asserted-by":"publisher","award":["GUIKE AA23026010"],"award-info":[{"award-number":["GUIKE AA23026010"]}],"id":[{"id":"10.13039\/501100018571","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018571","name":"Specific Research Project of Guangxi for Research Bases and Talents","doi-asserted-by":"publisher","award":["GXQH202408"],"award-info":[{"award-number":["GXQH202408"]}],"id":[{"id":"10.13039\/501100018571","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004607","name":"Natural Science Foundation of Guangxi Province","doi-asserted-by":"publisher","award":["2023JJB140603"],"award-info":[{"award-number":["2023JJB140603"]}],"id":[{"id":"10.13039\/501100004607","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011833","name":"Guangxi University of Chinese Medicine","doi-asserted-by":"publisher","award":["2022BS008"],"award-info":[{"award-number":["2022BS008"]}],"id":[{"id":"10.13039\/501100011833","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011833","name":"Guangxi University of Chinese Medicine","doi-asserted-by":"publisher","award":["2023JQ002"],"award-info":[{"award-number":["2023JQ002"]}],"id":[{"id":"10.13039\/501100011833","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82404938"],"award-info":[{"award-number":["82404938"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82204590"],"award-info":[{"award-number":["82204590"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Phytomedicine"],"published-print":{"date-parts":[[2025,3]]},"DOI":"10.1016\/j.phymed.2025.156407","type":"journal-article","created":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T19:37:08Z","timestamp":1737142628000},"page":"156407","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":36,"special_numbering":"C","title":["Anemoside B4 targets NEK7 to inhibit NLRP3 inflammasome activation and alleviate MSU-induced acute gouty arthritis by modulating the NF-\u03baB signaling pathway"],"prefix":"10.1016","volume":"138","author":[{"given":"Xinghua","family":"Ni","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinqin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujie","family":"Ning","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-4480-1686","authenticated-orcid":false,"given":"Qian","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shang","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulin","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shilin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8590-4364","authenticated-orcid":false,"given":"Renyikun","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9179-750X","authenticated-orcid":false,"given":"Hongwei","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.phymed.2025.156407_bib0001","first-page":"1234","article-title":"A comprehensive review on gout: The epidemiological trends, pathophysiology, clinical presentation, diagnosis and treatment. JPMA","volume":"71","author":"Ashiq","year":"2021","journal-title":"The Journal of the Pakistan Medical Association"},{"key":"10.1016\/j.phymed.2025.156407_bib0002","article-title":"Monosodium Urate Crystal-Induced Pyroptotic Cell Death in Neutrophil and Macrophage Facilitates the Pathological Progress of Gout","volume":"20","author":"Chen","year":"2024","journal-title":"Small."},{"key":"10.1016\/j.phymed.2025.156407_bib0003","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1038\/s41419-019-2157-1","article-title":"NEK7 interacts with NLRP3 to modulate the pyroptosis in inflammatory bowel disease via NF-\u03baB signaling","volume":"10","author":"Chen","year":"2019","journal-title":"Cell Death. Dis."},{"key":"10.1016\/j.phymed.2025.156407_bib0004","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1186\/s11658-023-00462-9","article-title":"The NLRP3 inflammasome: contributions to inflammation-related diseases","volume":"28","author":"Chen","year":"2023","journal-title":"Cell Mol. Biol. Lett."},{"key":"10.1016\/j.phymed.2025.156407_bib0005","first-page":"26","article-title":"Loganin Alleviates Gout Inflammation by Suppressing NLRP3 Inflammasome Activation and Mitochondrial Damage","author":"Choi","year":"2021","journal-title":"Molecules."},{"key":"10.1016\/j.phymed.2025.156407_bib0006","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2024.1412022","article-title":"Anemoside B4 attenuates abdominal aortic aneurysm by limiting smooth muscle cell transdifferentiation and its mediated inflammation","volume":"15","author":"Chu","year":"2024","journal-title":"Front. Immunol."},{"key":"10.1016\/j.phymed.2025.156407_bib0007","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2023.1178662","article-title":"Gasdermin D-mediated pyroptosis: mechanisms, diseases, and inhibitors","volume":"14","author":"Dai","year":"2023","journal-title":"Front. Immunol."},{"key":"10.1016\/j.phymed.2025.156407_bib0008","doi-asserted-by":"crossref","DOI":"10.1016\/j.lfs.2023.121766","article-title":"Polydatin ameliorates inflammation and oxidative stress associated with MSU-induced gouty arthritis in mice by regulating PPAR-\u03b3 and ferritin activation","volume":"326","author":"Du","year":"2023","journal-title":"Life Sci."},{"key":"10.1016\/j.phymed.2025.156407_bib0009","series-title":"Gout, StatPearls","author":"Fenando","year":"2024"},{"key":"10.1016\/j.phymed.2025.156407_bib0011","doi-asserted-by":"crossref","DOI":"10.1016\/j.intimp.2021.107375","article-title":"Curcumin analogue AI-44 alleviates MSU-induced gouty arthritis in mice via inhibiting cathepsin B-mediated NLRP3 inflammasome activation","volume":"93","author":"Gu","year":"2021","journal-title":"Int. Immunopharmacol."},{"key":"10.1016\/j.phymed.2025.156407_bib0012","doi-asserted-by":"crossref","DOI":"10.1016\/j.envres.2023.116980","article-title":"The molecular perspective on the melanoma and genome engineering of T-cells in targeting therapy","volume":"237","author":"Hajibabaie","year":"2023","journal-title":"Environ. Res."},{"key":"10.1016\/j.phymed.2025.156407_bib0013","article-title":"Types of Cell Death from a Molecular Perspective","volume":"12","author":"Hajibabaie","year":"2023","journal-title":"Biology. (Basel)"},{"key":"10.1016\/j.phymed.2025.156407_bib0014","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1186\/s13020-020-00350-w","article-title":"Anemoside B4 protects against Klebsiella pneumoniae- and influenza virus FM1-induced pneumonia via the TLR4\/Myd88 signaling pathway in mice","volume":"15","author":"He","year":"2020","journal-title":"Chin. Med."},{"key":"10.1016\/j.phymed.2025.156407_bib0015","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.phymed.2018.10.035","article-title":"Anemoside B4 attenuates nephrotoxicity of cisplatin without reducing anti-tumor activity of cisplatin","volume":"56","author":"He","year":"2019","journal-title":"Phytomedicine: international journal of phytotherapy and phytopharmacology"},{"key":"10.1016\/j.phymed.2025.156407_bib0016","doi-asserted-by":"crossref","DOI":"10.1155\/2020\/8706898","article-title":"HSP60 Regulates Monosodium Urate Crystal-Induced Inflammation by Activating the TLR4-NF-\u03baB-MyD88 Signaling Pathway and Disrupting Mitochondrial Function","volume":"2020","author":"Huang","year":"2020","journal-title":"Oxid. Med. Cell Longev."},{"key":"10.1016\/j.phymed.2025.156407_bib0017","article-title":"Entrectinib inhibits NLRP3 inflammasome and inflammatory diseases by directly targeting NEK7","volume":"4","author":"Jin","year":"2023","journal-title":"Cell Rep. Med."},{"key":"10.1016\/j.phymed.2025.156407_bib0018","doi-asserted-by":"crossref","DOI":"10.1016\/j.phymed.2019.152934","article-title":"Anti-inflammatory and immune-modulatory properties of anemoside B4 isolated from Pulsatilla chinensis in vivo","volume":"64","author":"Kang","year":"2019","journal-title":"Phytomedicine: international journal of phytotherapy and phytopharmacology"},{"key":"10.1016\/j.phymed.2025.156407_bib0019","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1042\/BST20230549","article-title":"New insights into Gasdermin D pore formation","volume":"52","author":"Kappelhoff","year":"2024","journal-title":"Biochem. Soc. Trans."},{"key":"10.1016\/j.phymed.2025.156407_bib0020","doi-asserted-by":"crossref","DOI":"10.1016\/j.envres.2023.117168","article-title":"System biology analysis to develop diagnostic biomarkers, monitoring pathological indexes, and novel therapeutic approaches for immune targeting based on maggot bioactive compounds and polyphenolic cocktails in mice with gastric cancer","volume":"238","author":"Kaviani","year":"2023","journal-title":"Environ. Res."},{"key":"10.1016\/j.phymed.2025.156407_bib0021","doi-asserted-by":"crossref","first-page":"140","DOI":"10.4078\/jrd.2022.29.3.140","article-title":"The Mechanism of the NLRP3 Inflammasome Activation and Pathogenic Implication in the Pathogenesis of Gout","volume":"29","author":"Kim","year":"2022","journal-title":"Journal of rheumatic diseases"},{"key":"10.1016\/j.phymed.2025.156407_bib0022","article-title":"Natural Products as a Novel Therapeutic Strategy for NLRP3 Inflammasome-Mediated Gout","volume":"13","author":"Lee","year":"2022","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.phymed.2025.156407_bib0023","first-page":"1466","article-title":"Pharmacodynamic study of Pulsatilla saponin B4 suppository in treatment of gout in rats","volume":"47","author":"Liang","year":"2024","journal-title":"Drug Evaluation Research"},{"key":"10.1016\/j.phymed.2025.156407_bib0024","doi-asserted-by":"crossref","DOI":"10.3390\/biomedicines10061250","article-title":"NF-\u03baB: A Double-Edged Sword Controlling Inflammation","volume":"10","author":"Liu","year":"2022","journal-title":"Biomedicines."},{"key":"10.1016\/j.phymed.2025.156407_bib0025","doi-asserted-by":"crossref","first-page":"74","DOI":"10.5582\/bst.2020.01029","article-title":"NEK7: a potential therapy target for NLRP3-related diseases","volume":"14","author":"Liu","year":"2020","journal-title":"Biosci. Trends."},{"key":"10.1016\/j.phymed.2025.156407_bib0026","article-title":"The triterpenoid saponin content difference is associated with the two type oxidosqualene cyclase gene copy numbers of Pulsatilla chinensis and Pulsatilla cernua","volume":"14","author":"Liu","year":"2023","journal-title":"Front. Plant Sci."},{"key":"10.1016\/j.phymed.2025.156407_bib0027","article-title":"Role of NLRP3 in the pathogenesis and treatment of gout arthritis","volume":"14","author":"Liu","year":"2023","journal-title":"Front. Immunol."},{"key":"10.1016\/j.phymed.2025.156407_bib0028","doi-asserted-by":"crossref","first-page":"7385","DOI":"10.1021\/acs.jmedchem.4c00222","article-title":"Discovery of a New Anti-Inflammatory Agent from Anemoside B4 Derivatives and Its Therapeutic Effect on Colitis by Targeting Pyruvate Carboxylase","volume":"67","author":"Lv","year":"2024","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.phymed.2025.156407_bib0029","unstructured":"Lv, S., 2020. Study on the Mechanism of Pulchinenoside b4 Against Gouty Arthritis (GA) Based on Metabolomics."},{"key":"10.1016\/j.phymed.2025.156407_bib0030","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1124\/pharmrev.122.000629","article-title":"Pharmacological Inhibition of the NLRP3 Inflammasome: Structure, Molecular Activation, and Inhibitor-NLRP3 Interaction","volume":"75","author":"Ma","year":"2023","journal-title":"Pharmacol. Rev."},{"key":"10.1016\/j.phymed.2025.156407_bib0031","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1016\/j.semnephrol.2020.12.001","volume":"40","author":"Narang","year":"2020","journal-title":"Pathophysiology of Gout. Seminars in nephrology"},{"key":"10.1016\/j.phymed.2025.156407_bib0032","article-title":"A novel NLRP3 inhibitor as a therapeutic agent against monosodium urate-induced gout","volume":"14","author":"Park","year":"2023","journal-title":"Front. Immunol."},{"key":"10.1016\/j.phymed.2025.156407_bib0033","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1016\/j.gendis.2023.01.009","article-title":"Fantastic voyage: The journey of NLRP3 inflammasome activation","volume":"11","author":"Que","year":"2024","journal-title":"Genes. Dis."},{"key":"10.1016\/j.phymed.2025.156407_bib0034","doi-asserted-by":"crossref","DOI":"10.1016\/j.phymed.2023.155302","article-title":"Exploration of the immuno-adjuvant effect and mechanism of Anemoside B4 through network pharmacology and experiment verification","volume":"124","author":"Shen","year":"2024","journal-title":"Phytomedicine: international journal of phytotherapy and phytopharmacology"},{"key":"10.1016\/j.phymed.2025.156407_bib0035","doi-asserted-by":"crossref","DOI":"10.1016\/j.intimp.2023.109977","article-title":"A review on gout: Looking back and looking ahead","volume":"117","author":"Tao","year":"2023","journal-title":"Int. Immunopharmacol."},{"key":"10.1016\/j.phymed.2025.156407_bib0036","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1007\/s10787-022-01026-7","article-title":"NEK7: a new target for the treatment of multiple tumors and chronic inflammatory diseases","volume":"30","author":"Wang","year":"2022","journal-title":"Inflammopharmacology."},{"key":"10.1016\/j.phymed.2025.156407_bib0037","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1093\/jmcb\/mjz020","article-title":"Mitochondrial ROS promote macrophage pyroptosis by inducing GSDMD oxidation","volume":"11","author":"Wang","year":"2019","journal-title":"J. Mol. Cell Biol."},{"key":"10.1016\/j.phymed.2025.156407_bib0038","first-page":"106","article-title":"Anemoside B4 inhibits SARS-CoV-2 replication in vitro and in vivo","volume":"16","author":"Xiao","year":"2024","journal-title":"Chin. Herb. Med."},{"key":"10.1016\/j.phymed.2025.156407_bib0039","first-page":"409","article-title":"Pachymaran Alleviates Acute Gouty Arthritis by Inhibiting NLRP3 Inflammasome Activation and Pyroptosis","volume":"30","author":"Yan","year":"2024","journal-title":"Altern. Ther. Health Med."},{"key":"10.1016\/j.phymed.2025.156407_bib0040","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1038\/s41392-023-01687-y","article-title":"The role of inflammasomes in human diseases and their potential as therapeutic targets","volume":"9","author":"Yao","year":"2024","journal-title":"Signal. Transduct. Target. Ther."},{"key":"10.1016\/j.phymed.2025.156407_bib0041","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2316551121","article-title":"Inhibition of NLRP3 inflammasome activation by A20 through modulation of NEK7","volume":"121","author":"Yu","year":"2024","journal-title":"Proc. Natl. Acad. Sci. u S. a"},{"key":"10.1016\/j.phymed.2025.156407_bib0042","doi-asserted-by":"crossref","DOI":"10.1155\/2020\/7502301","article-title":"Anemoside B4 Protects against Acute Lung Injury by Attenuating Inflammation through Blocking NLRP3 Inflammasome Activation and TLR4 Dimerization","volume":"2020","author":"Yuan","year":"2020","journal-title":"J. Immunol. Res."},{"key":"10.1016\/j.phymed.2025.156407_bib0043","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1021\/acs.jmedchem.0c01743","article-title":"Berberine Directly Targets the NEK7 Protein to Block the NEK7-NLRP3 Interaction and Exert Anti-inflammatory Activity","volume":"64","author":"Zeng","year":"2021","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.phymed.2025.156407_bib0044","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1186\/s13020-019-0274-5","article-title":"Comparative analysis of the complete chloroplast genome sequences of six species of Pulsatilla Miller","volume":"14","author":"Zhang","year":"2019","journal-title":"Ranunculaceae. Chinese medicine"},{"key":"10.1016\/j.phymed.2025.156407_bib0045","doi-asserted-by":"crossref","DOI":"10.3389\/fcvm.2022.1059576","article-title":"The role of mitochondria-associated membranes mediated ROS on NLRP3 inflammasome in cardiovascular diseases","volume":"9","author":"Zhao","year":"2022","journal-title":"Front. Cardiovasc. Med."},{"key":"10.1016\/j.phymed.2025.156407_bib0046","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/s13020-022-00613-8","article-title":"Pharmacological activities and molecular mechanisms of Pulsatilla saponins","volume":"17","author":"Zhong","year":"2022","journal-title":"Chin. Med."}],"updated-by":[{"DOI":"10.1016\/j.phymed.2025.156819","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000}},{"DOI":"10.1016\/j.phymed.2026.157892","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T00:00:00Z","timestamp":1770681600000}},{"DOI":"10.1016\/j.phymed.2026.157892","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000}}],"container-title":["Phytomedicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0944711325000467?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0944711325000467?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T17:47:10Z","timestamp":1776102430000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0944711325000467"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3]]},"references-count":45,"alternative-id":["S0944711325000467"],"URL":"https:\/\/doi.org\/10.1016\/j.phymed.2025.156407","relation":{},"ISSN":["0944-7113"],"issn-type":[{"value":"0944-7113","type":"print"}],"subject":[],"published":{"date-parts":[[2025,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Anemoside B4 targets NEK7 to inhibit NLRP3 inflammasome activation and alleviate MSU-induced acute gouty arthritis by modulating the NF-\u03baB signaling pathway","name":"articletitle","label":"Article Title"},{"value":"Phytomedicine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.phymed.2025.156407","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier GmbH.","name":"copyright","label":"Copyright"}],"article-number":"156407"}}