{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T17:42:09Z","timestamp":1793382129141,"version":"4.5.0"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100018938","name":"Administration of Traditional Chinese Medicine of Jilin Province","doi-asserted-by":"publisher","award":["20241125"],"award-info":[{"award-number":["20241125"]}],"id":[{"id":"10.13039\/100018938","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004000","name":"Guangzhou Municipal Science and Technology Program key projects","doi-asserted-by":"publisher","award":["YN2023MB01"],"award-info":[{"award-number":["YN2023MB01"]}],"id":[{"id":"10.13039\/501100004000","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004000","name":"Guangzhou Municipal Science and Technology Program key projects","doi-asserted-by":"publisher","award":["2025A03J4083"],"award-info":[{"award-number":["2025A03J4083"]}],"id":[{"id":"10.13039\/501100004000","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["BJ2022KY01"],"award-info":[{"award-number":["BJ2022KY01"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2022A1515220131"],"award-info":[{"award-number":["2022A1515220131"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2023A1515012626"],"award-info":[{"award-number":["2023A1515012626"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2024A1515012137"],"award-info":[{"award-number":["2024A1515012137"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Phytomedicine"],"published-print":{"date-parts":[[2025,6]]},"DOI":"10.1016\/j.phymed.2025.156705","type":"journal-article","created":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T09:52:50Z","timestamp":1743501170000},"page":"156705","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Gusongan capsule enhances osteogenic differentiation to mitigate bone loss in ovariectomized rats via the TLR2\/NF-\u03baB pathway"],"prefix":"10.1016","volume":"141","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1311-8641","authenticated-orcid":false,"given":"Lingfeng","family":"Zeng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7079-1336","authenticated-orcid":false,"given":"Jinlong","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuoxu","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanghui","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junzheng","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4596-6111","authenticated-orcid":false,"given":"Jianke","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8657-2269","authenticated-orcid":false,"given":"Weiyi","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1943-3880","authenticated-orcid":false,"given":"Jun","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7599-2628","authenticated-orcid":false,"given":"Guihong","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.phymed.2025.156705_bib0001","doi-asserted-by":"crossref","DOI":"10.1016\/j.intimp.2024.112738","article-title":"Saccharomyces boulardii (cncm i-745) alleviates collagen-induced arthritis by partially maintaining intestinal mucosal integrity through tlr2\/myd88\/nf-kappab pathway inhibition","volume":"139","author":"Ahmad","year":"2024","journal-title":"Int. Immunopharmacol."},{"issue":"3","key":"10.1016\/j.phymed.2025.156705_bib0002","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1111\/iej.14015","article-title":"Tlr2 agonist prevents the progression of periapical lesions in mice by reducing osteoclast activity and regulating the frequency of tregs","volume":"57","author":"Almeida","year":"2024","journal-title":"Int. Endod. J."},{"key":"10.1016\/j.phymed.2025.156705_bib0003","article-title":"Anti-osteoclast effect of exportin-1 inhibitor eltanexor on osteoporosis depends on nuclear accumulation of i\u03bab\u03b1\u2013nf-\u03bab p65 complex","volume":"13","author":"Chen","year":"2022","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.phymed.2025.156705_bib0004","article-title":"Osteoporosis treatment: current drugs and future developments","volume":"15","author":"Chen","year":"2024","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.phymed.2025.156705_bib0005","doi-asserted-by":"crossref","DOI":"10.1016\/j.taap.2024.116922","article-title":"Flavokawain b inhibits nf-kappab inflammatory signaling pathway activation in inflammatory bowel disease by targeting tlr2","volume":"486","author":"Chen","year":"2024","journal-title":"Toxicol. Appl. Pharm."},{"key":"10.1016\/j.phymed.2025.156705_bib0006","article-title":"Cardiovascular safety of romosozumab compared to commonly used anti-osteoporosis medications in postmenopausal osteoporosis: a systematic review and network meta-analysis of randomized controlled trials","author":"Cheng","year":"2024","journal-title":"Drug Saf."},{"issue":"10","key":"10.1016\/j.phymed.2025.156705_bib0007","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.1093\/jbmr\/zjae119","article-title":"Goal-directed osteoporosis treatment: asbmr\/bhof task force position statement 2024","volume":"39","author":"Cosman","year":"2024","journal-title":"J. Bone Miner. Res."},{"issue":"1","key":"10.1016\/j.phymed.2025.156705_bib0008","doi-asserted-by":"crossref","first-page":"ITC1","DOI":"10.7326\/AITC202401160","article-title":"Osteoporosis","volume":"177","author":"Ensrud","year":"2024","journal-title":"Ann. Intern. Med."},{"issue":"4","key":"10.1016\/j.phymed.2025.156705_bib0009","doi-asserted-by":"crossref","first-page":"2373","DOI":"10.1111\/odi.14712","article-title":"Lactobacillus rhamnosus inhibits osteoclast differentiation by suppressing thetlr2 \/nf-\u03bab pathway","volume":"30","author":"Fu","year":"2024","journal-title":"Oral Dis."},{"key":"10.1016\/j.phymed.2025.156705_bib0010","doi-asserted-by":"crossref","DOI":"10.1016\/j.micpath.2023.106165","article-title":"Myricetin exerts anti-biofilm activity and attenuates osteomyelitis by inhibiting the tlr2\/mapk pathway in experimental mice","volume":"182","author":"Gao","year":"2023","journal-title":"Microb. Pathogen."},{"issue":"2","key":"10.1016\/j.phymed.2025.156705_bib0011","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1093\/intimm\/dxz063","article-title":"Igg immune complexes withstaphylococcus aureus protein a enhance osteoclast differentiation and bone resorption by stimulating fc receptors and tlr2","volume":"32","author":"Kamohara","year":"2020","journal-title":"Int. Immunol."},{"key":"10.1016\/j.phymed.2025.156705_bib0012","first-page":"299","article-title":"Low-molecular-weight estrogenic phytoprotein suppresses osteoporosis development through positive modulation of skeletal estrogen receptors","volume":"42","author":"Kubi","year":"2024","journal-title":"Bioact. Mater."},{"issue":"9","key":"10.1016\/j.phymed.2025.156705_bib0013","doi-asserted-by":"crossref","first-page":"3583","DOI":"10.7150\/thno.96174","article-title":"Extracellular vesicles from apoptotic bmscs ameliorate osteoporosis via transporting regenerative signals","volume":"14","author":"Li","year":"2024","journal-title":"Theranostics"},{"key":"10.1016\/j.phymed.2025.156705_bib0014","doi-asserted-by":"crossref","DOI":"10.3389\/fendo.2022.866641","article-title":"Mechanism and experimental verification of luteolin for the treatment of osteoporosis based on network pharmacology","volume":"13","author":"Liang","year":"2022","journal-title":"Front. Endocrinol."},{"issue":"1","key":"10.1016\/j.phymed.2025.156705_bib0015","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1186\/s13018-023-03761-1","article-title":"Network pharmacology identifies fisetin as a treatment for osteoporosis that activates the wnt\/\u03b2-catenin signaling pathway in bmscs","volume":"18","author":"Liang","year":"2023","journal-title":"J. Orthop. Surg. Res."},{"key":"10.1016\/j.phymed.2025.156705_bib0016","doi-asserted-by":"crossref","DOI":"10.3389\/fmed.2023.1256238","article-title":"Longbie capsules reduce bone loss in the subchondral bone of rats with comorbid osteoporosis and osteoarthritis by regulating metabolite alterations","volume":"10","author":"Liang","year":"2023","journal-title":"Front. Med."},{"issue":"W1","key":"10.1016\/j.phymed.2025.156705_bib0017","doi-asserted-by":"crossref","first-page":"W159","DOI":"10.1093\/nar\/gkac394","article-title":"Cb-dock2: improved protein\u2013ligand blind docking by integrating cavity detection, docking and homologous template fitting","volume":"50","author":"Liu","year":"2022","journal-title":"Nucleic Acids Res."},{"issue":"23","key":"10.1016\/j.phymed.2025.156705_bib0018","doi-asserted-by":"crossref","first-page":"e70247","DOI":"10.1111\/jcmm.70247","article-title":"Lipoteichoic acid rescued age-related bone loss by enhancing neuroendocrine and growth hormone secretion through TLR2\/COX2\/PGE2 signalling pathway","volume":"28","author":"Liu","year":"2024","journal-title":"J. Cell Mol. Med."},{"issue":"12","key":"10.1016\/j.phymed.2025.156705_bib0019","doi-asserted-by":"crossref","first-page":"2369","DOI":"10.1007\/s00198-019-05101-8","article-title":"Percutaneous vertebroplasty versus non-operative treatment for osteoporotic vertebral compression fractures: a meta-analysis of randomized controlled trials","volume":"30","author":"Lou","year":"2019","journal-title":"Osteoporosis Int."},{"issue":"39","key":"10.1016\/j.phymed.2025.156705_bib0020","doi-asserted-by":"crossref","first-page":"E1333","DOI":"10.1503\/cmaj.221647","article-title":"Clinical practice guideline for management of osteoporosis and fracture prevention in canada: 2023 update","volume":"195","author":"Morin","year":"2023","journal-title":"Can. Med. Assoc. J."},{"issue":"10","key":"10.1016\/j.phymed.2025.156705_bib0021","doi-asserted-by":"crossref","first-page":"8077","DOI":"10.1007\/s00210-024-03124-5","article-title":"Fisetin mitigates letrozole-induced polycystic ovarian syndrome in rats: crosstalk of ampk\/pi3k\/akt-mediated-nrf2 antioxidant defense mechanism and the inflammasome nlrp3\/nf-\u03bab p65\/il-1\u03b2 signaling pathways","volume":"397","author":"Moustafa","year":"2024","journal-title":"Naunyn Schmiedebergs Arch. Pharmacol."},{"issue":"1","key":"10.1016\/j.phymed.2025.156705_bib0022","first-page":"323","article-title":"Network pharmacology, molecular docking, and in vitro study on aspilia pluriseta against prostate cancer","volume":"24","author":"Okpako","year":"2024","journal-title":"Bmc Complement. Med."},{"issue":"18","key":"10.1016\/j.phymed.2025.156705_bib0023","doi-asserted-by":"crossref","first-page":"3793","DOI":"10.1113\/JP280389","article-title":"The arrive guidelines 2.0: updated guidelines for reporting animal research","volume":"598","author":"Percie Du Sert","year":"2020","journal-title":"J. Physiol."},{"issue":"6","key":"10.1016\/j.phymed.2025.156705_bib0024","article-title":"Xbp1 facilitating nf-\u03bab-p65 nuclear translocation promotes macrophage-originated sterile inflammation via regulating mt2 transcription in the ischemia\/reperfusion liver","volume":"18","author":"Rao","year":"2024","journal-title":"Cell Mol. Gastroenter."},{"issue":"1","key":"10.1016\/j.phymed.2025.156705_bib0025","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1186\/s13018-021-02772-0","article-title":"The global prevalence of osteoporosis in the world: a comprehensive systematic review and meta-analysis","volume":"16","author":"Salari","year":"2021","journal-title":"J. Orthop. Surg. Res."},{"issue":"1","key":"10.1016\/j.phymed.2025.156705_bib0026","doi-asserted-by":"crossref","DOI":"10.1080\/14756366.2024.2398551","article-title":"Novel alloxazine analogues: design, synthesis, and antitumour efficacy enhanced by kinase screening, molecular docking, and adme studies","volume":"39","author":"Samaha","year":"2024","journal-title":"J. Enzym. Inhib. Med. Ch."},{"key":"10.1016\/j.phymed.2025.156705_bib0027","first-page":"1","article-title":"Exercised accelerated the production of muscle-derived kynurenic acid in skeletal muscle and alleviated the postmenopausal osteoporosis through the gpr35\/nf\u03bab p65 pathway","volume":"35","author":"Shi","year":"2022","journal-title":"J. Orthop. Transl."},{"issue":"5","key":"10.1016\/j.phymed.2025.156705_bib0028","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1111\/omi.12307","article-title":"Filifactor alocis -derived extracellular vesicles inhibit osteogenesis through tlr2 signaling","volume":"35","author":"Song","year":"2020","journal-title":"Mol. Oral. Microbiol."},{"key":"10.1016\/j.phymed.2025.156705_bib0029","doi-asserted-by":"crossref","DOI":"10.1007\/s00198-024-07260-9","article-title":"Association between renal function and fracture incidence during treatment with teriparatide or alendronate: an exploratory subgroup analysis of the japanese osteoporosis intervention trial-05","author":"Takeuchi","year":"2024","journal-title":"Osteoporosis Int."},{"issue":"3","key":"10.1016\/j.phymed.2025.156705_bib0030","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1538\/expanim.18-0003","article-title":"The validity of anesthetic protocols for the surgical procedure of castration in rats","volume":"67","author":"Tsukamoto","year":"2018","journal-title":"Exp. Anim."},{"issue":"5","key":"10.1016\/j.phymed.2025.156705_bib0031","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1007\/s00223-020-00790-4","article-title":"An exopolysaccharide produced by bifidobacterium longum 35624\u00ae inhibits osteoclast formation via a TLR2-dependent mechanism","volume":"108","author":"Wallimann","year":"2021","journal-title":"Calcified Tissue Int."},{"key":"10.1016\/j.phymed.2025.156705_bib0032","article-title":"Therapeutic potential of chinese medicinal herbs stimulating osteogenic differentiation of bone marrow-derived mesenchymal stem cells in osteoporosis","volume":"15","author":"Wang","year":"2024","journal-title":"Front. Pharmacol."},{"issue":"1","key":"10.1016\/j.phymed.2025.156705_bib0033","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1186\/s13287-018-0809-1","article-title":"Lrrc15 promotes osteogenic differentiation of mesenchymal stem cells by modulating p65 cytoplasmic\/nuclear translocation","volume":"9","author":"Wang","year":"2018","journal-title":"Stem Cell Res. Ther."},{"key":"10.1016\/j.phymed.2025.156705_bib0034","doi-asserted-by":"crossref","DOI":"10.1016\/j.phymed.2021.153743","article-title":"Shaoyao decoction attenuates dss-induced ulcerative colitis, macrophage and nlrp3 inflammasome activation through the mkp1\/nf-\u03bab pathway","volume":"92","author":"Wei","year":"2021","journal-title":"Phytomedicine"},{"key":"10.1016\/j.phymed.2025.156705_bib0035","article-title":"Celastrol ameliorates osteoarthritis via regulating TLR2\/NF-\u03bab signaling pathway","volume":"13","author":"Yang","year":"2022","journal-title":"Front. Pharmacol."},{"issue":"6","key":"10.1016\/j.phymed.2025.156705_bib0036","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1038\/s41374-018-0036-5","article-title":"Tsg-6 secreted by bone marrow mesenchymal stem cells attenuates intervertebral disc degeneration by inhibiting the tlr2\/nf-\u03bab signaling pathway","volume":"98","author":"Yang","year":"2018","journal-title":"Lab. Invest."},{"issue":"6","key":"10.1016\/j.phymed.2025.156705_bib0037","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1007\/s13770-023-00561-w","article-title":"Reactive oxygen species scavenging hydrogel regulates stem cell behavior and promotes bone healing in osteoporosis","volume":"20","author":"Ye","year":"2023","journal-title":"Tissue Eng. Regen. Med."},{"issue":"1","key":"10.1016\/j.phymed.2025.156705_bib0038","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1002\/ptr.7610","article-title":"Alpinetin alleviates osteoporosis by promoting osteogenic differentiation inbmscs by triggering autophagy viapka \/mtor \/ulk1 signaling","volume":"37","author":"Zeng","year":"2023","journal-title":"Phytother. Res."},{"key":"10.1016\/j.phymed.2025.156705_bib0039","doi-asserted-by":"crossref","DOI":"10.1016\/j.jep.2024.117709","article-title":"Study on anti-inflammatory effect of shangkehuangshui in vitro and in vivo based on tlr4\/tlr2-nf-\u03bab signaling pathway","volume":"323","author":"Zhang","year":"2024","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.phymed.2025.156705_bib0040","first-page":"177","article-title":"Macf1 overexpression in bmscs alleviates senile osteoporosis in mice through tcf4\/mir-335\u20135p signaling pathway","volume":"39","author":"Zhang","year":"2023","journal-title":"J. Orthop. Transl."},{"issue":"12","key":"10.1016\/j.phymed.2025.156705_bib0041","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1016\/j.jsps.2021.10.009","article-title":"Icariin regulates mir-23a-3p-mediated osteogenic differentiation of bmscs via bmp-2\/smad5\/runx2 and wnt\/\u03b2-catenin pathways in osteonecrosis of the femoral head","volume":"29","author":"Zhang","year":"2021","journal-title":"Saudi Pharm. J."},{"key":"10.1016\/j.phymed.2025.156705_bib0042","doi-asserted-by":"crossref","DOI":"10.1016\/j.jep.2023.117253","article-title":"Active components and mechanisms of total flavonoids from rhizoma drynariae in enhancing cranial bone regeneration: an investigation employing serum pharmacochemistry and network pharmacology approaches","volume":"319","author":"Zhao","year":"2024","journal-title":"J. Ethnopharmacol."},{"issue":"1","key":"10.1016\/j.phymed.2025.156705_bib0043","first-page":"3329","article-title":"The use of TLR2 modified bmscs for enhanced bone regeneration in the inflammatory micro-environment","volume":"47","author":"Zhou","year":"2019","journal-title":"Artif. Cell Nanomed. B"}],"container-title":["Phytomedicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0944711325003459?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0944711325003459?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T08:49:04Z","timestamp":1762332544000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0944711325003459"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":43,"alternative-id":["S0944711325003459"],"URL":"https:\/\/doi.org\/10.1016\/j.phymed.2025.156705","relation":{},"ISSN":["0944-7113"],"issn-type":[{"value":"0944-7113","type":"print"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Gusongan capsule enhances osteogenic differentiation to mitigate bone loss in ovariectomized rats via the TLR2\/NF-\u03baB pathway","name":"articletitle","label":"Article Title"},{"value":"Phytomedicine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.phymed.2025.156705","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier GmbH.","name":"copyright","label":"Copyright"}],"article-number":"156705"}}