{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T02:53:24Z","timestamp":1790823204867,"version":"4.1.0"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82370553"],"award-info":[{"award-number":["82370553"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81973538"],"award-info":[{"award-number":["81973538"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003452","name":"Innovation and Technology Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003452","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012479","name":"General Research Fund of Shanghai Normal University","doi-asserted-by":"publisher","award":["12100323"],"award-info":[{"award-number":["12100323"]}],"id":[{"id":"10.13039\/501100012479","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005847","name":"Health and Medical Research Fund","doi-asserted-by":"publisher","award":["17182661"],"award-info":[{"award-number":["17182661"]}],"id":[{"id":"10.13039\/501100005847","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Phytomedicine"],"published-print":{"date-parts":[[2025,9]]},"DOI":"10.1016\/j.phymed.2025.157040","type":"journal-article","created":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T02:59:50Z","timestamp":1751425190000},"page":"157040","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Butyrate-producing commensal bacteria mediates the efficacy of herbal medicine JCM-16021 on abdominal pain in diarrhea-predominant irritable bowel syndrome: a randomized clinical trial"],"prefix":"10.1016","volume":"145","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3907-5910","authenticated-orcid":false,"given":"Lixiang","family":"Zhai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-5426-6827","authenticated-orcid":false,"given":"Cho Wing","family":"Lo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujun","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanting","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica YL","family":"Ching","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziwan","family":"Ning","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gengyu","family":"Bao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuqin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chung Wah","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wai Ching","family":"Lam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kam Leung","family":"Chan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pui Yan","family":"Lam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing Yao","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda L.D.","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei Hua","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Koh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pui Kuan","family":"Cheong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhixiu","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengyuan","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4932-499X","authenticated-orcid":false,"given":"Ling","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier Hoi Leong","family":"Wong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin CY","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoxiang","family":"Bian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.phymed.2025.157040_bib0001","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1038\/s41586-023-05829-8","article-title":"Gut enterochromaffin cells drive visceral pain and anxiety","volume":"616","author":"Bayrer","year":"2023","journal-title":"Nature"},{"key":"10.1016\/j.phymed.2025.157040_bib0002","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.65088","article-title":"Integrating taxonomic, functional, and strain-level profiling of diverse microbial communities with bioBakery 3","volume":"10","author":"Beghini","year":"2021","journal-title":"Elife"},{"key":"10.1016\/j.phymed.2025.157040_bib0003","doi-asserted-by":"crossref","first-page":"483","DOI":"10.14309\/ajg.0000000000000518","article-title":"Efficacy of eluxadoline in irritable bowel syndrome with diarrhea","volume":"115","author":"Black","year":"2020","journal-title":"Am. J. Gastroenterol."},{"key":"10.1016\/j.phymed.2025.157040_bib0004","doi-asserted-by":"crossref","first-page":"26482","DOI":"10.1073\/pnas.2006065117","article-title":"Fecal transplantation and butyrate improve neuropathic pain, modify immune cell profile, and gene expression in the PNS of obese mice","volume":"117","author":"Bonomo","year":"2020","journal-title":"Proc. Natl. Acad. Sci. u S. a"},{"key":"10.1016\/j.phymed.2025.157040_bib0005","doi-asserted-by":"crossref","first-page":"112","DOI":"10.7326\/M16-2977","article-title":"CONSORT extension for Chinese herbal medicine formulas 2017: recommendations, explanation, and elaboration","volume":"167","author":"Cheng","year":"2017","journal-title":"Ann. Intern. Med."},{"key":"10.1016\/j.phymed.2025.157040_bib0006","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1001\/jama.2015.0954","article-title":"Irritable bowel syndrome: a clinical review","volume":"313","author":"Chey","year":"2015","journal-title":"JAMa"},{"key":"10.1016\/j.phymed.2025.157040_bib0007","doi-asserted-by":"crossref","first-page":"797","DOI":"10.3389\/fpsyt.2020.00797","article-title":"Placebo responses and Placebo effects in functional gastrointestinal disorders","volume":"11","author":"Enck","year":"2020","journal-title":"Front. Psychiatry"},{"key":"10.1016\/j.phymed.2025.157040_bib0008","doi-asserted-by":"crossref","DOI":"10.3390\/nu15092211","article-title":"Short-chain fatty-acid-producing bacteria: key components of the Human gut microbiota","volume":"15","author":"Fusco","year":"2023","journal-title":"Nutrients."},{"key":"10.1016\/j.phymed.2025.157040_bib0009","doi-asserted-by":"crossref","first-page":"1404","DOI":"10.1053\/j.gastro.2023.08.052","article-title":"Traditional medicine Pien Tze Huang suppresses colorectal tumorigenesis through restoring gut microbiota and metabolites","volume":"165","author":"Gou","year":"2023","journal-title":"Gastroenterology"},{"key":"10.1016\/j.phymed.2025.157040_bib0010","doi-asserted-by":"crossref","first-page":"G1","DOI":"10.1152\/ajpgi.00048.2011","article-title":"Functional analysis of colonic bacterial metabolism: relevant to health?","volume":"302","author":"Hamer","year":"2012","journal-title":"Am. J. Physiol. Gastrointest. Liver. Physiol."},{"key":"10.1016\/j.phymed.2025.157040_bib0011","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.chom.2024.11.013","article-title":"Roseburia intestinalis-derived butyrate alleviates neuropathic pain","volume":"33","author":"Jiang","year":"2025","journal-title":"Cell Host. Microbe"},{"key":"10.1016\/j.phymed.2025.157040_bib0012","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1053\/j.gastro.2013.04.056","article-title":"Short-chain fatty acids activate GPR41 and GPR43 on intestinal epithelial cells to promote inflammatory responses in mice","volume":"145","author":"Kim","year":"2013","journal-title":"Gastroenterology"},{"key":"10.1016\/j.phymed.2025.157040_bib0013","first-page":"2815","article-title":"ATF2 - at the crossroad of nuclear and cytosolic functions","volume":"125","author":"Lau","year":"2012","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.phymed.2025.157040_bib0014","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1053\/j.gastro.2022.04.017","article-title":"AGA clinical practice guideline on the pharmacological management of irritable bowel syndrome with diarrhea","volume":"163","author":"Lembo","year":"2022","journal-title":"Gastroenterology"},{"key":"10.1016\/j.phymed.2025.157040_bib0015","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1056\/NEJMoa1505180","article-title":"Eluxadoline for irritable bowel syndrome with diarrhea","volume":"374","author":"Lembo","year":"2016","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.phymed.2025.157040_bib0016","doi-asserted-by":"crossref","DOI":"10.1080\/19490976.2021.1968257","article-title":"Microbiota metabolite butyrate constrains neutrophil functions and ameliorates mucosal inflammation in inflammatory bowel disease","volume":"13","author":"Li","year":"2021","journal-title":"Gut. Microbes."},{"key":"10.1016\/j.phymed.2025.157040_bib0017","doi-asserted-by":"crossref","first-page":"8998","DOI":"10.1523\/JNEUROSCI.6684-10.2011","article-title":"Essential roles of enteric neuronal serotonin in gastrointestinal motility and the development\/survival of enteric dopaminergic neurons","volume":"31","author":"Li","year":"2011","journal-title":"J. Neurosci."},{"key":"10.1016\/j.phymed.2025.157040_bib0018","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1007\/s10565-020-09518-4","article-title":"Sodium butyrate modulates gut microbiota and immune response in colorectal cancer liver metastatic mice","volume":"36","author":"Ma","year":"2020","journal-title":"Cell Biol. Toxicol."},{"issue":"Suppl 1","key":"10.1016\/j.phymed.2025.157040_bib0019","first-page":"S63","article-title":"Review article: current and future treatment approaches for IBS with diarrhoea (IBS-D) and IBS mixed pattern (IBS-M)","volume":"54","author":"Nee","year":"2021","journal-title":"Aliment. Pharmacol. Ther."},{"key":"10.1016\/j.phymed.2025.157040_bib0020","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-019-56132-4","article-title":"Butyrate inhibits visceral allodynia and colonic hyperpermeability in rat models of irritable bowel syndrome","volume":"9","author":"Nozu","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.phymed.2025.157040_bib0021","unstructured":"Oksanen, J., Blanchet, F.G., Kindt, R., Legendre, P., Minchin, P.R., O'Hara, R.B., Simpson, G.L., S\u00f3lymos, P., Stevens, M.H.H., Wagner, H., 2012. vegan: community Ecology Package."},{"issue":"Suppl 1","key":"10.1016\/j.phymed.2025.157040_bib0022","first-page":"S75","article-title":"Review article: current and future treatment approaches for pain in IBS","volume":"54","author":"Paine","year":"2021","journal-title":"Aliment. Pharmacol. Ther."},{"key":"10.1016\/j.phymed.2025.157040_bib0023","article-title":"The ARRIVE guidelines 2.0: updated guidelines for reporting animal research","volume":"4","author":"Percie du Sert","year":"2020","journal-title":"BMJ Open. Sci."},{"key":"10.1016\/j.phymed.2025.157040_bib0024","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1136\/gutjnl-2020-321530","article-title":"Effect of resolvins on sensitisation of TRPV1 and visceral hypersensitivity in IBS","volume":"70","author":"Perna","year":"2021","journal-title":"Gut"},{"key":"10.1016\/j.phymed.2025.157040_bib0025","doi-asserted-by":"crossref","DOI":"10.1155\/2012\/239638","article-title":"JCM-16021, a Chinese herbal formula, attenuated visceral hyperalgesia in TNBS-induced postinflammatory irritable bowel syndrome through reducing colonic EC cell hyperplasia and serotonin availability in rats","volume":"2012","author":"Qin","year":"2012","journal-title":"Evid. Based. Complement. Alternat. Med."},{"key":"10.1016\/j.phymed.2025.157040_bib0026","doi-asserted-by":"crossref","first-page":"603","DOI":"10.14309\/ajg.0000000000003042","article-title":"Fecal proteolytic bacteria and staphylococcal superantigens are associated with abdominal pain severity in irritable bowel syndrome","volume":"120","author":"Quan","year":"2025","journal-title":"Am. J. Gastroenterol."},{"key":"10.1016\/j.phymed.2025.157040_bib0027","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.phrs.2015.11.026","article-title":"Sodium butyrate and its synthetic amide derivative modulate nociceptive behaviors in mice","volume":"103","author":"Russo","year":"2016","journal-title":"Pharmacol. Res."},{"key":"10.1016\/j.phymed.2025.157040_bib0028","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1136\/gutjnl-2024-333530","article-title":"Butyrate-producing faecalibacterium prausnitzii suppresses natural killer\/T-cell lymphoma by dampening the JAK-STAT pathway","volume":"74","author":"Shi","year":"2025","journal-title":"Gut"},{"key":"10.1016\/j.phymed.2025.157040_bib0029","first-page":"569","article-title":"The microbial metabolites, short-chain fatty acids, regulate colonic treg cell homeostasis","volume":"341","author":"Smith","year":"2013","journal-title":"Science (1979)"},{"key":"10.1016\/j.phymed.2025.157040_bib0030","doi-asserted-by":"crossref","first-page":"13145","DOI":"10.1073\/pnas.1412008111","article-title":"Commensal bacteria protect against food allergen sensitization","volume":"111","author":"Stefka","year":"2014","journal-title":"Proc. Natl. Acad. Sci. u S. a"},{"key":"10.1016\/j.phymed.2025.157040_bib0031","doi-asserted-by":"crossref","DOI":"10.3390\/ijms232113486","article-title":"A concise review of liquid chromatography-mass spectrometry-based quantification methods for short chain fatty acids as endogenous biomarkers","volume":"23","author":"Trivedi","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.phymed.2025.157040_bib0032","doi-asserted-by":"crossref","first-page":"3214","DOI":"10.2741\/3445","article-title":"Microbial diversity in the human intestine and novel insights from metagenomics","volume":"14","author":"Ventura","year":"2009","journal-title":"Front. Biosci. (Landmark. Ed)"},{"key":"10.1016\/j.phymed.2025.157040_bib0033","doi-asserted-by":"crossref","first-page":"11648","DOI":"10.1073\/pnas.1917597117","article-title":"Microbiota-derived butyrate dynamically regulates intestinal homeostasis through regulation of actin-associated protein synaptopodin","volume":"117","author":"Wang","year":"2020","journal-title":"Proc. Natl. Acad. Sci. u S. a"},{"key":"10.1016\/j.phymed.2025.157040_bib0034","doi-asserted-by":"crossref","DOI":"10.1016\/j.chom.2022.11.006","article-title":"Ruminococcus gnavus plays a pathogenic role in diarrhea-predominant irritable bowel syndrome by increasing serotonin biosynthesis","volume":"31","author":"Zhai","year":"2023","journal-title":"Cell Host. Microbe"},{"key":"10.1016\/j.phymed.2025.157040_bib0035","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1186\/s13020-021-00530-2","article-title":"Efficacy and safety of Chinese medicine JCM-16021 for diarrhea-predominant irritable bowel syndrome: study protocol for a multi-center, randomized, double-blind, placebo controlled clinical trial","volume":"16","author":"Zheng","year":"2021","journal-title":"Chin. Med."}],"container-title":["Phytomedicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0944711325006798?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0944711325006798?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T08:52:57Z","timestamp":1762332777000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0944711325006798"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":35,"alternative-id":["S0944711325006798"],"URL":"https:\/\/doi.org\/10.1016\/j.phymed.2025.157040","relation":{},"ISSN":["0944-7113"],"issn-type":[{"value":"0944-7113","type":"print"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Butyrate-producing commensal bacteria mediates the efficacy of herbal medicine JCM-16021 on abdominal pain in diarrhea-predominant irritable bowel syndrome: a randomized clinical trial","name":"articletitle","label":"Article Title"},{"value":"Phytomedicine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.phymed.2025.157040","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier GmbH.","name":"copyright","label":"Copyright"}],"article-number":"157040"}}