{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T20:49:12Z","timestamp":1788382152222,"version":"build-2803163510"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004775","name":"Natural Science Foundation of Gansu Province","doi-asserted-by":"publisher","award":["24JRRA349"],"award-info":[{"award-number":["24JRRA349"]}],"id":[{"id":"10.13039\/501100004775","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012899","name":"Lanzhou University","doi-asserted-by":"publisher","award":["CY2023-QN-B02"],"award-info":[{"award-number":["CY2023-QN-B02"]}],"id":[{"id":"10.13039\/100012899","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009620","name":"Gansu Province Science and Technology Department","doi-asserted-by":"publisher","award":["25JRRA1271"],"award-info":[{"award-number":["25JRRA1271"]}],"id":[{"id":"10.13039\/501100009620","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Phytomedicine"],"published-print":{"date-parts":[[2025,11]]},"DOI":"10.1016\/j.phymed.2025.157267","type":"journal-article","created":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T22:33:29Z","timestamp":1758062009000},"page":"157267","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"special_numbering":"C","title":["Combined transcriptomics and network pharmacology to elucidate the mechanisms of rutin in treating ischemic stroke rat"],"prefix":"10.1016","volume":"148","author":[{"given":"Ting","family":"Zheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taotao","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufeng","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4224-7047","authenticated-orcid":false,"given":"Jianqing","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4981-4995","authenticated-orcid":false,"given":"Weiyuan","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manxia","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.phymed.2025.157267_bib0001","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbadis.2018.09.012","article-title":"Current advances in ischemic stroke research and therapies","volume":"1866","author":"Barthels","year":"2020","journal-title":"Biochim. Biophys. Acta Mol. Basis. Dis."},{"key":"10.1016\/j.phymed.2025.157267_bib0002","doi-asserted-by":"crossref","DOI":"10.1016\/j.fct.2024.114626","article-title":"RUTIN, a widely consumed flavonoid, that commonly induces hormetic effects","volume":"187","author":"Calabrese","year":"2024","journal-title":"Food Chem. Toxicol."},{"key":"10.1016\/j.phymed.2025.157267_bib0003","doi-asserted-by":"crossref","DOI":"10.1212\/WNL.0000000000208098","article-title":"Inflammatory biomarkers and stroke subtype: an important new frontier","volume":"102","author":"Chaturvedi","year":"2024","journal-title":"Neurology."},{"key":"10.1016\/j.phymed.2025.157267_bib0004","doi-asserted-by":"crossref","DOI":"10.1016\/j.phrs.2020.104877","article-title":"Therapeutic targets of oxidative\/nitrosative stress and neuroinflammation in ischemic stroke: applications for natural product efficacy with omics and systemic biology","volume":"158","author":"Chen","year":"2020","journal-title":"Pharmacol. Res."},{"key":"10.1016\/j.phymed.2025.157267_bib0005","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2023.107866","article-title":"Rutin alleviates ventilator-induced lung injury by inhibiting NLRP3 inflammasome activation","volume":"26","author":"Chen","year":"2023","journal-title":"iScience"},{"key":"10.1016\/j.phymed.2025.157267_bib0006","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2025.1599167","article-title":"Molecular mechanisms of neuroprotective effect of rutin","volume":"16","author":"Chunmei","year":"2025","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.phymed.2025.157267_bib0007","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1038\/s41589-019-0277-7","article-title":"MCC950 directly targets the NLRP3 ATP-hydrolysis motif for inflammasome inhibition","volume":"15","author":"Coll","year":"2019","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.phymed.2025.157267_bib0008","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1038\/nm.3806","article-title":"A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases","volume":"21","author":"Coll","year":"2015","journal-title":"Nat. Med."},{"key":"10.1016\/j.phymed.2025.157267_bib0009","doi-asserted-by":"crossref","first-page":"W357","DOI":"10.1093\/nar\/gkz382","article-title":"SwissTargetPrediction: updated data and new features for efficient prediction of protein targets of small molecules","volume":"47","author":"Daina","year":"2019","journal-title":"Nucleic. Acids. Res."},{"key":"10.1016\/j.phymed.2025.157267_bib0010","doi-asserted-by":"crossref","DOI":"10.3390\/ijms25020891","article-title":"Neuroprotection during thrombectomy for acute ischemic stroke: a review of future therapies","volume":"25","author":"Dammavalam","year":"2024","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.phymed.2025.157267_bib0011","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/bs.apcsb.2017.07.003","article-title":"Analysis of protein interactions by surface plasmon resonance","volume":"110","author":"Drescher","year":"2018","journal-title":"Adv. Protein Chem. Struct. Biol."},{"key":"10.1016\/j.phymed.2025.157267_bib0012","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1080\/10408398.2020.1829541","article-title":"The anticancer potential of the dietary polyphenol rutin: current status, challenges, and perspectives","volume":"62","author":"Farha","year":"2022","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"10.1016\/j.phymed.2025.157267_bib0013","doi-asserted-by":"crossref","first-page":"C487","DOI":"10.1152\/ajpcell.00600.2023","article-title":"Cenicriviroc prevents dysregulation of astrocyte\/endothelial cross talk induced by ischemia and HIV-1 via inhibiting the NLRP3 inflammasome and pyroptosis","volume":"326","author":"Fattakhov","year":"2024","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"10.1016\/j.phymed.2025.157267_bib0014","doi-asserted-by":"crossref","first-page":"W726","DOI":"10.1093\/nar\/gkac297","article-title":"SuperPred 3.0: drug classification and target prediction-a machine learning approach","volume":"50","author":"Gallo","year":"2022","journal-title":"Nucleic. Acids. Res."},{"key":"10.1016\/j.phymed.2025.157267_bib0015","article-title":"Mechanisms of immune response and cell death in ischemic stroke and their regulation by natural compounds","volume":"14","author":"Gong","year":"2023","journal-title":"Front. Immunol."},{"key":"10.1016\/j.phymed.2025.157267_bib0016","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.2174\/1389557523666230125104101","article-title":"An overview of biosynthetic pathway and therapeutic potential of rutin","volume":"23","author":"Goyal","year":"2023","journal-title":"Mini. Rev. Med. Chem."},{"key":"10.1016\/j.phymed.2025.157267_bib0017","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1007\/s11626-024-00893-4","article-title":"Rutin alleviates Sjogren's syndrome via CaR\/NLRP3\/NF-kappaB signal pathway","volume":"60","author":"He","year":"2024","journal-title":"In. Vitro Cell Dev. Biol. Anim."},{"key":"10.1016\/j.phymed.2025.157267_bib0018","doi-asserted-by":"crossref","DOI":"10.3390\/ijms241914771","article-title":"Ursolic acid alleviates neuroinflammation after intracerebral hemorrhage by mediating microglial pyroptosis via the NF-kappaB\/NLRP3\/GSDMD pathway","volume":"24","author":"Lei","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.phymed.2025.157267_bib0019","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1136\/svn-2023-002320","article-title":"STING mediates microglial pyroptosis via interaction with NLRP3 in cerebral ischaemic stroke","volume":"9","author":"Li","year":"2024","journal-title":"Stroke Vasc. Neurol."},{"key":"10.1016\/j.phymed.2025.157267_bib0020","doi-asserted-by":"crossref","first-page":"W159","DOI":"10.1093\/nar\/gkac394","article-title":"CB-Dock2: improved protein-ligand blind docking by integrating cavity detection, docking and homologous template fitting","volume":"50","author":"Liu","year":"2022","journal-title":"Nucleic. Acids. Res."},{"key":"10.1016\/j.phymed.2025.157267_bib0021","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1161\/01.STR.20.1.84","article-title":"Reversible middle cerebral artery occlusion without craniectomy in rats","volume":"20","author":"Longa","year":"1989","journal-title":"Stroke"},{"key":"10.1016\/j.phymed.2025.157267_bib0022","doi-asserted-by":"crossref","DOI":"10.1016\/j.biopha.2023.115847","article-title":"Flavonoids and ischemic stroke-induced neuroinflammation: focus on the glial cells","volume":"170","author":"Lu","year":"2024","journal-title":"Biomed. PharmacOther"},{"key":"10.1016\/j.phymed.2025.157267_bib0023","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1186\/s12974-022-02501-2","article-title":"Intermittent theta-burst stimulation improves motor function by inhibiting neuronal pyroptosis and regulating microglial polarization via TLR4\/NFkappaB\/NLRP3 signaling pathway in cerebral ischemic mice","volume":"19","author":"Luo","year":"2022","journal-title":"J. Neuroinflammation."},{"key":"10.1016\/j.phymed.2025.157267_bib0024","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/S0014-5793(97)00467-5","article-title":"Bioavailability of rutin and quercetin in rats","volume":"409","author":"Manach","year":"1997","journal-title":"FEBS Lett."},{"key":"10.1016\/j.phymed.2025.157267_bib0025","doi-asserted-by":"crossref","first-page":"230S","DOI":"10.1093\/ajcn\/81.1.230S","article-title":"Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies","volume":"81","author":"Manach","year":"2005","journal-title":"Am. J. Clin. Nutr."},{"key":"10.1016\/j.phymed.2025.157267_bib0026","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1111\/j.1365-2990.2012.01252.x","article-title":"A new method for focal transient cerebral ischaemia by distal compression of the middle cerebral artery","volume":"38","author":"Morancho","year":"2012","journal-title":"Neuropathol. Appl. Neurobiol."},{"key":"10.1016\/j.phymed.2025.157267_bib0027","doi-asserted-by":"crossref","first-page":"W26","DOI":"10.1093\/nar\/gku477","article-title":"SuperPred: update on drug classification and target prediction","volume":"42","author":"Nickel","year":"2014","journal-title":"Nucleic. Acids. Res."},{"key":"10.1016\/j.phymed.2025.157267_bib0028","doi-asserted-by":"crossref","DOI":"10.3390\/ijms232314784","article-title":"Enzymatically modified isoquercitrin: production, metabolism, bioavailability, toxicity, pharmacology, and related molecular mechanisms","volume":"23","author":"Owczarek-Januszkiewicz","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.phymed.2025.157267_bib0029","doi-asserted-by":"crossref","DOI":"10.1016\/j.ejphar.2022.174973","article-title":"Rutin protects hemorrhagic stroke development via supressing oxidative stress and inflammatory events in a zebrafish model","volume":"925","author":"Rana","year":"2022","journal-title":"Eur. J. Pharmacol."},{"key":"10.1016\/j.phymed.2025.157267_bib0030","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/1758-2946-6-13","article-title":"TCMSP: a database of systems pharmacology for drug discovery from herbal medicines","volume":"6","author":"Ru","year":"2014","journal-title":"J. Cheminform."},{"key":"10.1016\/j.phymed.2025.157267_bib0031","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1517\/13543784.2013.805744","article-title":"Rutin: therapeutic potential and recent advances in drug delivery","volume":"22","author":"Sharma","year":"2013","journal-title":"Expert. Opin. Investig. Drugs"},{"key":"10.1016\/j.phymed.2025.157267_bib0032","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1007\/s12017-023-08753-2","article-title":"Inflammasome activation mediates apoptotic and pyroptotic death in astrocytes under ischemic conditions","volume":"25","author":"Wong","year":"2023","journal-title":"Neuromolecular. Med."},{"key":"10.1016\/j.phymed.2025.157267_bib0033","doi-asserted-by":"crossref","first-page":"D1110","DOI":"10.1093\/nar\/gky1021","article-title":"SymMap: an integrative database of traditional Chinese medicine enhanced by symptom mapping","volume":"47","author":"Wu","year":"2019","journal-title":"Nucleic. Acids. Res."},{"key":"10.1016\/j.phymed.2025.157267_bib0034","article-title":"Sophorolipid suppresses LPS-induced inflammation in RAW264.7 cells through the NF-kappaB signaling pathway","volume":"27","author":"Xu","year":"2022","journal-title":"Molecules."},{"key":"10.1016\/j.phymed.2025.157267_bib0035","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1186\/s12974-025-03492-6","article-title":"IFP35, a novel DAMP, aggravates neuroinflammation following acute ischemic stroke via TLR4\/NF-kappaB\/NLRP3 signaling","volume":"22","author":"Zhang","year":"2025","journal-title":"J. Neuroinflammation."}],"container-title":["Phytomedicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0944711325009067?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0944711325009067?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T06:15:56Z","timestamp":1773123356000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0944711325009067"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":35,"alternative-id":["S0944711325009067"],"URL":"https:\/\/doi.org\/10.1016\/j.phymed.2025.157267","relation":{},"ISSN":["0944-7113"],"issn-type":[{"value":"0944-7113","type":"print"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Combined transcriptomics and network pharmacology to elucidate the mechanisms of rutin in treating ischemic stroke rat","name":"articletitle","label":"Article Title"},{"value":"Phytomedicine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.phymed.2025.157267","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier GmbH. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"157267"}}