{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T16:51:21Z","timestamp":1777567881839,"version":"3.51.4"},"reference-count":30,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T00:00:00Z","timestamp":1380585600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T00:00:00Z","timestamp":1380585600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["256716"],"award-info":[{"award-number":["256716"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004564","name":"Ministarstvo Prosvete, Nauke i Tehnolo\u0161kog Razvoja","doi-asserted-by":"publisher","award":["172049"],"award-info":[{"award-number":["172049"]}],"id":[{"id":"10.13039\/501100004564","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Phytochemistry"],"published-print":{"date-parts":[[2013,10]]},"DOI":"10.1016\/j.phytochem.2013.06.006","type":"journal-article","created":{"date-parts":[[2013,7,3]],"date-time":"2013-07-03T19:31:47Z","timestamp":1372879907000},"page":"53-59","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":22,"special_numbering":"C","title":["Biochemical and immunological characterization of a recombinantly-produced antifungal cysteine proteinase inhibitor from green kiwifruit (Actinidia deliciosa)"],"prefix":"10.1016","volume":"94","author":[{"given":"Milica","family":"Popovic","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uros","family":"Andjelkovic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lidija","family":"Burazer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Buko","family":"Lindner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arnd","family":"Petersen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marija","family":"Gavrovic-Jankulovic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.phytochem.2013.06.006_b0005","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1111\/j.1432-1033.1992.tb17365.x","article-title":"Corn kernel cysteine proteinase inhibitor as a novel cystatin superfamily member of plant origin. Molecular cloning and expression studies","volume":"209","author":"Abe","year":"1992","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.phytochem.2013.06.006_b0010","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1093\/protein\/6.4.383","article-title":"Evaluation of secondary structure of proteins from uv circular dichroism spectra using an unsupervised learning neural network","volume":"6","author":"Andrade","year":"1993","journal-title":"Protein Eng."},{"key":"10.1016\/j.phytochem.2013.06.006_b0015","series-title":"Finding Protein Sequences Using Prowl. Current Protocols in Bioinformatics","author":"Beavis","year":"2004"},{"key":"10.1016\/j.phytochem.2013.06.006_b0150","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1021\/jf802966n","article-title":"Influence of the natural ripening stage, cold storage, and ethylene treatment on the rotein and IgE-bunding profiles of Green and Gold kiwi fruit extracts","volume":"57","author":"Ciardiello","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.phytochem.2013.06.006_b0020","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1023\/A:1010697204686","article-title":"A constitutive cystatin-encoding gene from barley (icy) responds differentially to abiotic stimuli","volume":"45","author":"Gaddour","year":"2001","journal-title":"Plant Mol. Biol."},{"key":"10.1016\/j.phytochem.2013.06.006_b0025","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.pep.2005.10.026","article-title":"Recombinant expression, purification, and functional analysis of two novel cystatins from sugarcane (Saccharum officinarum)","volume":"47","author":"Gianotti","year":"2006","journal-title":"Prot. Expression Purif."},{"key":"10.1016\/j.phytochem.2013.06.006_b0030","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.abb.2004.07.022","article-title":"Protein proteinase inhibitor genes in combat against insects, pests, and pathogens: natural and engineered phytoprotection","volume":"431","author":"Haq","year":"2004","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.phytochem.2013.06.006_b0035","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/0003-2697(82)90400-6","article-title":"Experimental errors and their effect on analyzing circular dichroism spectra of proteins","volume":"125","author":"Hennessey","year":"1982","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.phytochem.2013.06.006_b0040","doi-asserted-by":"crossref","first-page":"1515","DOI":"10.1021\/jf00008a030","article-title":"Isolation and partial characterization of a soybean cystatin cysteine proteinase inhibitor of coleopteran digestive proteolytic activity","volume":"39","author":"Hines","year":"1991","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.phytochem.2013.06.006_b0045","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1006\/abbi.1998.0875","article-title":"Structural and phylogenetic relationships among plant and animal cystatins","volume":"359","author":"Margis","year":"1998","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.phytochem.2013.06.006_b0050","doi-asserted-by":"crossref","first-page":"1821","DOI":"10.1093\/jxb\/eri172","article-title":"The strawberry gene Cyf1 encodes a phytocystatin with antifungal properties","volume":"56","author":"Martinez","year":"2005","journal-title":"J. Exp. Bot."},{"key":"10.1016\/j.phytochem.2013.06.006_b0055","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1104\/pp.109.146019","article-title":"Characterization of the entire cystatin gene family in barley and their target cathepsin l-like cysteine-proteases, partners in the hordein mobilization during seed germination","volume":"151","author":"Martinez","year":"2009","journal-title":"Plant Physiol."},{"key":"10.1016\/j.phytochem.2013.06.006_b0060","doi-asserted-by":"crossref","first-page":"2914","DOI":"10.1016\/j.febslet.2007.05.042","article-title":"Carboxy terminal extended phytocystatins are bifunctional inhibitors of papain and legumain cysteine proteinases","volume":"581","author":"Martinez","year":"2007","journal-title":"FEBS Lett."},{"key":"10.1016\/j.phytochem.2013.06.006_b0065","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1186\/1471-2148-8-198","article-title":"The origin and evolution of plant cystatins and their target cysteine proteinases indicate a complex functional relationship","volume":"8","author":"Martinez","year":"2008","journal-title":"BMC Evol. Biol."},{"key":"10.1016\/j.phytochem.2013.06.006_b0070","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.bbaexp.2005.05.004","article-title":"Maize cystatins respond to developmental cues, cold stress and drought","volume":"1729","author":"Massonneau","year":"2005","journal-title":"Biochim. Biophys. Acta Gene Struct. Expression"},{"key":"10.1016\/j.phytochem.2013.06.006_b0075","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/0925-2312(94)90021-3","article-title":"Proteinotopic feature maps","volume":"6","author":"Merelo","year":"1994","journal-title":"Neurocomputing"},{"key":"10.1016\/j.phytochem.2013.06.006_b0080","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1111\/j.1432-1033.1996.0609h.x","article-title":"Soyacystatin, a novel cysteine proteinase inhibitor in soybean, is distinct in protein structure and gene organization from other cystatins of animal and plant origin","volume":"240","author":"Misaka","year":"1996","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.phytochem.2013.06.006_b0085","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1105\/tpc.108.064717","article-title":"Characterization of Solanum tuberosum multicystatin and its structural comparison with other cystatins","volume":"21","author":"Nissen","year":"2009","journal-title":"The Plant Cell"},{"key":"10.1016\/j.phytochem.2013.06.006_b0090","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1094\/MPMI.1999.12.7.624","article-title":"Antifungal activity of a plant cystatin","volume":"12","author":"Pernas","year":"1999","journal-title":"Mol. Plant\u2013Microbe Interact."},{"key":"10.1016\/j.phytochem.2013.06.006_b0095","doi-asserted-by":"crossref","first-page":"1235","DOI":"10.1023\/A:1006154829118","article-title":"A chestnut seed cystatin differentially effective against cysteine proteinases from closely related pests","volume":"38","author":"Pernas","year":"1998","journal-title":"Plant Mol. Biol."},{"key":"10.1016\/j.phytochem.2013.06.006_b0100","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1007\/s12088-012-0319-2","article-title":"In vitro antibacterial activity of cysteine protease inhibitor from kiwifruit (Actinidia deliciosa)","volume":"53","author":"Popovic","year":"2013","journal-title":"Indian J. Microbiol."},{"key":"10.1016\/j.phytochem.2013.06.006_b0105","doi-asserted-by":"crossref","first-page":"3072","DOI":"10.1002\/jsfa.5728","article-title":"In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit","volume":"92","author":"Popovic","year":"2012","journal-title":"J. Sci. Food Agric."},{"key":"10.1016\/j.phytochem.2013.06.006_b0110","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1002\/mnfr.200900035","article-title":"Cysteine proteinase inhibitor Act d 4 is a functional allergen contributing to the clinical symptoms of kiwifruit allergy","volume":"54","author":"Popovic","year":"2010","journal-title":"Mol. Nutr. Food Res."},{"key":"10.1016\/j.phytochem.2013.06.006_b0115","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.phytochem.2003.09.019","article-title":"Purification and characterization of phytocystatins from kiwifruit cortex and seeds","volume":"65","author":"Rassam","year":"2004","journal-title":"Phytochemistry"},{"key":"10.1016\/j.phytochem.2013.06.006_b0120","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1094\/PHYTO.2003.93.7.799","article-title":"Expression of Oryzacystatin I and II in Alfalfa increases resistance to the root-lesion nematode","volume":"93","author":"Samac","year":"2003","journal-title":"Phytopathology"},{"key":"10.1016\/j.phytochem.2013.06.006_b0125","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1111\/j.1467-7652.2009.00466.x","article-title":"Genetically pyramiding protease-inhibitor genes for dual broad-spectrum resistance against insect and phytopathogens in transgenic tobacco","volume":"8","author":"Senthilkumar","year":"2010","journal-title":"Plant Biotechnol. J."},{"key":"10.1016\/j.phytochem.2013.06.006_b0130","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1006\/abio.2001.5321","article-title":"Evaluation of the efficiency of in-gel digestion of proteins by peptide isotopic labeling and maldi mass spectrometry","volume":"296","author":"Shevchenko","year":"2001","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.phytochem.2013.06.006_b0135","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1016\/S0006-291X(02)02046-6","article-title":"A sugarcane cystatin: recombinant expression, purification, and antifungal activity","volume":"296","author":"Soares-Costa","year":"2002","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.phytochem.2013.06.006_b0140","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1016\/j.plaphy.2007.07.007","article-title":"Cloning of a cdna encoding a cystatin from grain amaranth (Amaranthus hypochondriacus) showing a tissue-specific expression that is modified by germination and abiotic stress","volume":"45","author":"Vald\u00e9s-Rodr\u00edguez","year":"2007","journal-title":"Plant Physiol. Biochem."},{"key":"10.1016\/j.phytochem.2013.06.006_b0145","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1002\/bip.20853","article-title":"Protein secondary structure analyses from circular dichroism spectroscopy: methods and reference databases","volume":"89","author":"Whitmore","year":"2008","journal-title":"Biopolymers"}],"container-title":["Phytochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0031942213002331?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0031942213002331?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T18:35:09Z","timestamp":1758479709000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0031942213002331"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,10]]},"references-count":30,"alternative-id":["S0031942213002331"],"URL":"https:\/\/doi.org\/10.1016\/j.phytochem.2013.06.006","relation":{},"ISSN":["0031-9422"],"issn-type":[{"value":"0031-9422","type":"print"}],"subject":[],"published":{"date-parts":[[2013,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Biochemical and immunological characterization of a recombinantly-produced antifungal cysteine proteinase inhibitor from green kiwifruit (Actinidia deliciosa)","name":"articletitle","label":"Article Title"},{"value":"Phytochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.phytochem.2013.06.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}