{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T13:15:32Z","timestamp":1784726132289,"version":"3.55.0"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Phytochemistry"],"published-print":{"date-parts":[[2021,4]]},"DOI":"10.1016\/j.phytochem.2021.112657","type":"journal-article","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T08:00:52Z","timestamp":1610697652000},"page":"112657","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Floro-pyrazolo[3,4-d]pyrimidine derivative as a novel plant activator induces two-pathway immune system"],"prefix":"10.1016","volume":"184","author":[{"given":"Qinjie","family":"Shi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanxia","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenjiang","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiping","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufang","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoju","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuhong","family":"Qian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.phytochem.2021.112657_bib1","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1016\/j.ejmech.2009.10.051","article-title":"Molecular iodine promoted synthesis of new pyrazolo[3,4-d]pyrimidine derivatives as potential antibacterial agents","volume":"45","author":"Bakavoli","year":"2010","journal-title":"Eur. J. Med. Chem."},{"key":"10.1016\/j.phytochem.2021.112657_bib2","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.envpol.2008.09.010","article-title":"Pesticide resistance from historical agricultural chemical exposure in Thamnocephalus platyurus (Crustacea: Anostraca)","volume":"157","author":"Brausch","year":"2009","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.phytochem.2021.112657_bib3","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1016\/j.cclet.2017.02.004","article-title":"The discovery of new scaffold of plant activators: from salicylic acid to benzotriazole","volume":"28","author":"Chang","year":"2017","journal-title":"Chin. Chem. Lett."},{"key":"10.1016\/j.phytochem.2021.112657_bib4","doi-asserted-by":"crossref","first-page":"1849","DOI":"10.1039\/C6MD00261G","article-title":"The discovery of new plant activators and scaffolds with potential induced systemic resistance: from jasmonic acid to pyrrolidone","volume":"7","author":"Chang","year":"2016","journal-title":"Med. Chem. Commun."},{"key":"10.1016\/j.phytochem.2021.112657_bib5","doi-asserted-by":"crossref","first-page":"5162","DOI":"10.1021\/jm050136d","article-title":"Novel, highly potent adenosine deaminase inhibitors containing the pyrazolo[3,4-d]pyrimidine ring system. Synthesis, Structure\u2212Activity relationships, and molecular modeling studies","volume":"48","author":"Da Settimo","year":"2005","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.phytochem.2021.112657_bib6","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1016\/j.cclet.2013.07.003","article-title":"Novel plant activators with thieno[2,3-d]-1,2,3-thiadiazole-6-carboxylate scaffold: synthesis and bioactivity","volume":"24","author":"Du","year":"2013","journal-title":"Chin. Chem. Lett."},{"key":"10.1016\/j.phytochem.2021.112657_bib7","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1021\/jf203974p","article-title":"Novel benzo-1,2,3-thiadiazole-7-carboxylate derivatives as plant activators and the development of their agricultural applications","volume":"60","author":"Du","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.phytochem.2021.112657_bib8","doi-asserted-by":"crossref","first-page":"3196","DOI":"10.1016\/j.bmcl.2017.05.015","article-title":"Pyrazolo[3,4-d]pyrimidines-loaded human serum albumin (HSA) nanoparticles: preparation, characterization and cytotoxicity evaluation against neuroblastoma cell line","volume":"27","author":"Fallacara","year":"2017","journal-title":"Bioorg. Med. Chem. Lett"},{"key":"10.1016\/j.phytochem.2021.112657_bib9","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1146\/annurev-arplant-042811-105606","article-title":"Systemic acquired resistance: turning local infection into global defense","volume":"64","author":"Fu","year":"2013","journal-title":"Annu. Rev. Plant Biol."},{"key":"10.1016\/j.phytochem.2021.112657_bib10","doi-asserted-by":"crossref","first-page":"4487","DOI":"10.1021\/jf030025s","article-title":"Systemic acquired resistance in crop Protection: from nature to a chemical approach","volume":"51","author":"Gozzo","year":"2003","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.phytochem.2021.112657_bib11","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1016\/j.pbi.2006.05.013","article-title":"Systemic immunity","volume":"9","author":"Grant","year":"2006","journal-title":"Curr. Opin. Plant Biol."},{"key":"10.1016\/j.phytochem.2021.112657_bib12","series-title":"A Convenient Synthesis of Fluorinated Pyrazolo[3,4-B]pyridine and Pyrazolo[3,4-D]pyrimidine Nucleosides. Synthesis-Stuttgart 2009","first-page":"731","author":"Iaroshenko","year":"2009"},{"key":"10.1016\/j.phytochem.2021.112657_bib13","doi-asserted-by":"crossref","first-page":"2899","DOI":"10.1021\/jo102579g","article-title":"Synthesis of fluorinated purine and 1-deazapurine glycosides as potential inhibitors of adenosine deaminase","volume":"76","author":"Iaroshenko","year":"2011","journal-title":"J. Org. Chem."},{"key":"10.1016\/j.phytochem.2021.112657_bib14","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.fjps.2016.02.002","article-title":"Pyrazolo[3,4-d]pyrimidine based scaffold derivatives targeting kinases as anticancer agents","volume":"2","author":"Ismail","year":"2016","journal-title":"Future J. Pharm. Sci."},{"key":"10.1016\/j.phytochem.2021.112657_bib15","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1093\/pcp\/pcm062","article-title":"Probenazole-induced accumulation of salicylic acid confers resistance to magnaporthe grisea in adult rice plants","volume":"48","author":"Iwai","year":"2007","journal-title":"Plant Cell Physiol."},{"key":"10.1016\/j.phytochem.2021.112657_bib16","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1104\/pp.18.01301","article-title":"Extracellular ATP shapes a defense-related transcriptome both independently and along with other defense signaling pathways","volume":"179","author":"Jewell","year":"2019","journal-title":"Plant Physiol."},{"key":"10.1016\/j.phytochem.2021.112657_bib17","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1046\/j.1365-313X.1996.10010071.x","article-title":"SABP2 resistance in Arabidopsis by activation of the systemic acquired resistance signal transduction pathway","volume":"10","author":"Lawton","year":"1996","journal-title":"Plant J."},{"key":"10.1016\/j.phytochem.2021.112657_bib18","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1080\/00397911.2014.996296","article-title":"A new efficient synthesis of 4-Alkoxy-1,6-diaryl-1H-pyrazolo[3,4-d]pyrimidine derivatives","volume":"45","author":"Liu","year":"2015","journal-title":"Synth. Commun."},{"key":"10.1016\/j.phytochem.2021.112657_bib19","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1002\/ps.636","article-title":"United States Department of Agriculture\u2013Agricultural Research Service research on improving host-plant resistance to pests","volume":"59","author":"Lynch","year":"2003","journal-title":"Pest Manag. Sci."},{"key":"10.1016\/j.phytochem.2021.112657_bib20","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1146\/annurev-phyto-082712-102340","article-title":"Induced systemic resistance by beneficial microbes","volume":"52","author":"Pieterse","year":"2014","journal-title":"Annu. Rev. Phytopathol."},{"key":"10.1016\/j.phytochem.2021.112657_bib21","doi-asserted-by":"crossref","first-page":"4071","DOI":"10.1093\/jxb\/erm263","article-title":"Jasmonates and its mimics differentially elicit systemic defence responses in Nicotiana attenuata","volume":"58","author":"Pluskota","year":"2007","journal-title":"J. Exp. Bot."},{"key":"10.1016\/j.phytochem.2021.112657_bib22","doi-asserted-by":"crossref","first-page":"2974","DOI":"10.2174\/138161212800672804","article-title":"Cyclin-dependent kinase inhibitors inspired by roscovitine: purine bioisosteres","volume":"18","author":"Radek","year":"2012","journal-title":"Curr. Pharmaceut. Des."},{"key":"10.1016\/j.phytochem.2021.112657_bib23","doi-asserted-by":"crossref","first-page":"7102","DOI":"10.1016\/j.bmc.2008.06.054","article-title":"Synthesis and antiviral evaluation of some new pyrazole and fused pyrazolopyrimidine derivatives","volume":"16","author":"Rashad","year":"2008","journal-title":"Bioorg. Med. Chem."},{"key":"10.1016\/j.phytochem.2021.112657_bib24","doi-asserted-by":"crossref","first-page":"5050","DOI":"10.1021\/acs.orglett.6b02499","article-title":"Synthesis of isothiazole via the rhodium-catalyzed transannulation of 1,2,3-thiadiazoles with nitriles","volume":"18","author":"Seo","year":"2016","journal-title":"Org. Lett."},{"key":"10.1016\/j.phytochem.2021.112657_bib25","doi-asserted-by":"crossref","first-page":"36204","DOI":"10.1039\/C9RA06892A","article-title":"3(2H)-pyridazinone derivatives: a new scaffold for novel plant activators","volume":"9","author":"Shi","year":"2019","journal-title":"RSC Adv."},{"key":"10.1016\/j.phytochem.2021.112657_bib26","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1016\/j.phytochem.2010.04.027","article-title":"Plant defense elicitors: analogues of jasmonoyl\u2013isoleucine conjugate","volume":"71","author":"Svoboda","year":"2010","journal-title":"Phytochemistry"},{"key":"10.1016\/j.phytochem.2021.112657_bib27","doi-asserted-by":"crossref","first-page":"3458","DOI":"10.1016\/j.febslet.2010.06.046","article-title":"SABP2, a methyl salicylate esterase is required for the systemic acquired resistance induced by acibenzolar-S-methyl in plants","volume":"584","author":"Tripathi","year":"2010","journal-title":"FEBS Lett."},{"key":"10.1016\/j.phytochem.2021.112657_bib28","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1584\/jpestics.31.161","article-title":"Development of a novel plant activator for rice diseases, tiadinil","volume":"31","author":"Tsubata","year":"2006","journal-title":"J. Pestic. Sci."},{"key":"10.1016\/j.phytochem.2021.112657_bib29","first-page":"645","article-title":"Acquired resistance in Arabidopsis","volume":"4","author":"Uknes","year":"1992","journal-title":"Plant Cell"},{"key":"10.1016\/j.phytochem.2021.112657_bib30","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1006\/pmpp.1999.0213","article-title":"The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins","volume":"55","author":"Van Loon","year":"1999","journal-title":"Physiol. Mol. Plant Pathol."},{"key":"10.1016\/j.phytochem.2021.112657_bib31","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1094\/MPMI-21-7-0919","article-title":"Differential effectiveness of microbially induced resistance against herbivorous insects in Arabidopsis","volume":"21","author":"Van Oosten","year":"2008","journal-title":"Mol. Plant Microbe Interact."},{"key":"10.1016\/j.phytochem.2021.112657_bib32","doi-asserted-by":"crossref","first-page":"4839","DOI":"10.1021\/jf0202436","article-title":"Development of new fungicides against magnaporthe grisea: synthesis and biological activity of pyrazolo[3,4-d][1,3]thiazine, pyrazolo[1,5-c][1,3,5]thiadiazine, and pyrazolo[3,4-d]pyrimidine derivatives","volume":"50","author":"Vicentini","year":"2002","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.phytochem.2021.112657_bib33","doi-asserted-by":"crossref","first-page":"7321","DOI":"10.1021\/jf801359f","article-title":"Synthesis and antifungal activities of novel 5-Amino-6-arylamino- 1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one derivatives","volume":"56","author":"Wang","year":"2008","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.phytochem.2021.112657_bib34","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1584\/jpestics.W12-20","article-title":"Problems of the insecticide resistance management for sustained insect pest control","volume":"37","author":"Yamamoto","year":"2012","journal-title":"J. Pesti. Sci."},{"key":"10.1016\/j.phytochem.2021.112657_bib35","doi-asserted-by":"crossref","first-page":"2165","DOI":"10.1016\/j.bmc.2018.03.017","article-title":"Design, synthesis and biological evaluation of novel 3-substituted pyrazolopyrimidine derivatives as potent Bruton's tyrosine kinase (BTK) inhibitors","volume":"26","author":"Zheng","year":"2018","journal-title":"Bioorg. Med. Chem."}],"container-title":["Phytochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0031942221000042?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0031942221000042?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T18:42:15Z","timestamp":1758480135000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0031942221000042"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4]]},"references-count":35,"alternative-id":["S0031942221000042"],"URL":"https:\/\/doi.org\/10.1016\/j.phytochem.2021.112657","relation":{},"ISSN":["0031-9422"],"issn-type":[{"value":"0031-9422","type":"print"}],"subject":[],"published":{"date-parts":[[2021,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Floro-pyrazolo[3,4-d]pyrimidine derivative as a novel plant activator induces two-pathway immune system","name":"articletitle","label":"Article Title"},{"value":"Phytochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.phytochem.2021.112657","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"112657"}}