{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T03:40:33Z","timestamp":1764906033823,"version":"3.44.0"},"reference-count":52,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T00:00:00Z","timestamp":1700092800000},"content-version":"am","delay-in-days":653,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Phytochemistry"],"published-print":{"date-parts":[[2022,2]]},"DOI":"10.1016\/j.phytochem.2021.113014","type":"journal-article","created":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T16:04:41Z","timestamp":1637078681000},"page":"113014","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Psilostachyins as trypanocidal compounds: Bioguided fractionation of Ambrosia tenuifolia chemically modified extract"],"prefix":"10.1016","volume":"194","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7127-2455","authenticated-orcid":false,"given":"Tonino G.","family":"Adessi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0576-6526","authenticated-orcid":false,"given":"Yamile","family":"Ana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8932-3409","authenticated-orcid":false,"given":"Cinthia C.","family":"Stempin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1943-4891","authenticated-orcid":false,"given":"M\u00f3nica C.","family":"Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabricio R.","family":"Bisogno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viviana E.","family":"Nicotra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6809-4723","authenticated-orcid":false,"given":"Manuela E.","family":"Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.phytochem.2021.113014_bib1","series-title":"Rational Drug Design: Methods and Protocols","first-page":"139","article-title":"Bioguided design of trypanosomicidal compounds: a successful strategy in drug discovery","author":"\u00c1lvarez Touron","year":"2018"},{"key":"10.1016\/j.phytochem.2021.113014_bib2","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1039\/b514294f","article-title":"Natural products to drugs: natural product-derived compounds in clinical trials","volume":"25","author":"Butler","year":"2008","journal-title":"Nat. Prod. Rep."},{"key":"10.1016\/j.phytochem.2021.113014_bib3","series-title":"Optimization in Drug Discovery: in Vitro Methods","first-page":"281","article-title":"Rapidly distinguishing reversible and time-dependent CYP450 inhibition using human liver microsomes, Co-incubation, and continuous fluorometric kinetic analyses","author":"Caldwell","year":"2014"},{"key":"10.1016\/j.phytochem.2021.113014_bib4","series-title":"Sesquiterpene Lactones: Structural Diversity and Their Biological Activities, Opportunity, Challenge and Scope of Natural Products in Medicinal Chemistry","first-page":"313","author":"Chaturvedi","year":"2011"},{"key":"10.1016\/j.phytochem.2021.113014_bib5","series-title":"Bioactive Natural Products, Chemistry and Biology","first-page":"321","article-title":"Sesquiterpene lactones: a versatile class of structurally diverse natural products and their semisynthetic analogs as potential anticancer agent","author":"Chaturvedi","year":"2015"},{"key":"10.1016\/j.phytochem.2021.113014_bib6","doi-asserted-by":"crossref","first-page":"5307","DOI":"10.1128\/AAC.00595-13","article-title":"Activities of psilostachyin A and cynaropicrin against Trypanosoma cruzi in vitro and in vivo","volume":"57","author":"da Silva","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.phytochem.2021.113014_bib7","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1076\/phbi.37.2.165.6077","article-title":"Sesquiterpene lactones from Ambrosia artemisiaefolia (asteraceae)","volume":"37","author":"David","year":"1999","journal-title":"Pharmaceut. Biol."},{"key":"10.1016\/j.phytochem.2021.113014_bib8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cbpa.2018.12.007","article-title":"Natural product derived privileged scaffolds in drug discovery","volume":"52","author":"Davison","year":"2019","journal-title":"Curr. Opin. Chem. Biol."},{"key":"10.1016\/j.phytochem.2021.113014_bib9","unstructured":"Declaration of the Global Chagas Disease Coalition. DNDi (Drugs for Neglegted Diseased Initiative), 2013 Securing Access to Current Treatments while Researching Better Options, PLOS Neglected Tropical Diseases."},{"key":"10.1016\/j.phytochem.2021.113014_bib10","doi-asserted-by":"crossref","first-page":"9295","DOI":"10.1021\/acs.jmedchem.6b00473","article-title":"Underexplored opportunities for natural products in drug discovery","volume":"59","author":"DeCorte","year":"2016","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.phytochem.2021.113014_bib11","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1007\/s00436-017-5731-4","article-title":"Assessment of the antiprotozoal activity of Pulicaria inuloides extracts, an Algerian medicinal plant: leishmanicidal bioguided fractionation","volume":"117","author":"Fadel","year":"2018","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.phytochem.2021.113014_bib12","first-page":"855","article-title":"PG490-88, a derivative of triptolide, causes tumor regression and sensitizes tumors to chemotherapy","volume":"2","author":"Fidler","year":"2003","journal-title":"Mol. Cancer Therapeut."},{"key":"10.1016\/j.phytochem.2021.113014_bib13","doi-asserted-by":"crossref","first-page":"3700","DOI":"10.1128\/AAC.00404-16","article-title":"Clomipramine and benznidazole act synergistically and ameliorate the outcome of experimental Chagas disease","volume":"60","author":"Garc\u00eda","year":"2016","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.phytochem.2021.113014_bib14","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.ejps.2018.04.034","article-title":"Multi-kinetic release of benznidazole-loaded multiparticulate drug delivery systems based on polymethacrylate interpolyelectrolyte complexes","volume":"120","author":"Garc\u00eda","year":"2018","journal-title":"Eur. J. Pharmaceut. Sci."},{"key":"10.1016\/j.phytochem.2021.113014_bib15","doi-asserted-by":"crossref","first-page":"3421","DOI":"10.1021\/ja01595a043","article-title":"Organic peracid oxidation of some enol esters involving Rearrangement1","volume":"78","author":"Gardner","year":"1956","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.phytochem.2021.113014_bib16","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1016\/j.drudis.2010.06.002","article-title":"What made sesquiterpene lactones reach cancer clinical trials?","volume":"15","author":"Ghantous","year":"2010","journal-title":"Drug Discov. Today"},{"key":"10.1016\/j.phytochem.2021.113014_bib17","doi-asserted-by":"crossref","first-page":"112327","DOI":"10.1016\/j.ejmech.2020.112327","article-title":"Epoxide containing molecules: a good or a bad drug design approach","volume":"201","author":"Gomes","year":"2020","journal-title":"Eur. J. Med. Chem."},{"key":"10.1016\/j.phytochem.2021.113014_bib18","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.apsb.2016.06.003","article-title":"The modification of natural products for medical use","volume":"7","author":"Guo","year":"2017","journal-title":"Acta Pharm. Sin. B"},{"key":"10.1016\/j.phytochem.2021.113014_bib19","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1016\/S0031-9422(00)85877-X","article-title":"Sesquiterpene lactones of some Ambrosia species","volume":"8","author":"Herz","year":"1969","journal-title":"Phytochemistry"},{"key":"10.1016\/j.phytochem.2021.113014_bib20","doi-asserted-by":"crossref","first-page":"12882","DOI":"10.1039\/C3RA45702H","article-title":"meta-Chloroperbenzoic acid (mCPBA): a versatile reagent in organic synthesis","volume":"4","author":"Hussain","year":"2014","journal-title":"RSC Adv."},{"year":"2009","series-title":"Tests for in Vitro Cytotoxicity","key":"10.1016\/j.phytochem.2021.113014_bib21"},{"key":"10.1016\/j.phytochem.2021.113014_bib22","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1039\/c0np00069h","article-title":"Natural products and Chagas' disease: a review of plant compounds studied for activity against Trypanosoma cruzi","volume":"28","author":"Izumi","year":"2011","journal-title":"Nat. Prod. Rep."},{"key":"10.1016\/j.phytochem.2021.113014_bib23","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1016\/j.phymed.2014.06.005","article-title":"Natural sesquiterpene lactones induce programmed cell death in Trypanosoma cruzi: a new therapeutic target?","volume":"21","author":"Jimenez","year":"2014","journal-title":"Phytomedicine"},{"key":"10.1016\/j.phytochem.2021.113014_bib24","doi-asserted-by":"crossref","first-page":"4377","DOI":"10.1016\/j.bmc.2011.05.009","article-title":"Generation of \u2018Unnatural Natural Product\u2019 library and identification of a small molecule inhibitor of XIAP","volume":"19","author":"Kawamura","year":"2011","journal-title":"Bioorg. Med. Chem."},{"key":"10.1016\/j.phytochem.2021.113014_bib25","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1021\/acs.jnatprod.7b00747","article-title":"Antiprotozoal sesquiterpene lactones and other constituents from Tarchonanthus camphoratus and Schkuhria pinnata","volume":"81","author":"Kimani","year":"2018","journal-title":"J. Nat. Prod."},{"key":"10.1016\/j.phytochem.2021.113014_bib26","doi-asserted-by":"crossref","DOI":"10.3390\/molecules23020248","article-title":"Sesquiterpene lactones from Vernonia cinerascens sch. Bip. And their in vitro antitrypanosomal activity","volume":"23","author":"Kimani","year":"2018","journal-title":"Molecules"},{"key":"10.1016\/j.phytochem.2021.113014_bib27","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1073\/pnas.0608438104","article-title":"Chemically engineered extracts as an alternative source of bioactive natural product-like compounds","volume":"104","author":"L\u00f3pez","year":"2007","journal-title":"Proc. Natl. Acad. Sci. Unit. States Am."},{"key":"10.1016\/j.phytochem.2021.113014_bib28","doi-asserted-by":"crossref","first-page":"1943","DOI":"10.1016\/S0040-4020(01)82268-7","article-title":"Psilostachyin B, a new sesquiterpene dilactone from Ambrosia psilostachya DC","volume":"22","author":"Mabry","year":"1966","journal-title":"Tetrahedron"},{"key":"10.1016\/j.phytochem.2021.113014_bib29","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1016\/j.tiv.2015.06.011","article-title":"Sesquiterpene lactones from Ambrosia spp. are active against a murine lymphoma cell line by inducing apoptosis and cell cycle arrest","volume":"29","author":"Martino","year":"2015","journal-title":"Toxicol. Vitro"},{"key":"10.1016\/j.phytochem.2021.113014_bib30","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1021\/co100073k","article-title":"Brominated extracts as source of bioactive compounds","volume":"13","author":"M\u00e9ndez","year":"2011","journal-title":"ACS Comb. Sci."},{"key":"10.1016\/j.phytochem.2021.113014_bib31","doi-asserted-by":"crossref","first-page":"2845","DOI":"10.1007\/s00044-017-1983-x","article-title":"Bioguided study of the in vitro parasitocidal effect on adult Hymenolepis nana of the Psidium sartorianum (O. Berg Nied.) fruit methanol extract","volume":"26","author":"Montes-Avila","year":"2017","journal-title":"Med. Chem. Res."},{"key":"10.1016\/j.phytochem.2021.113014_bib32","doi-asserted-by":"crossref","DOI":"10.1177\/1934578X1601101036","article-title":"Natural sesquiterpene lactones as potential trypanocidal therapeutic agents: a review","volume":"11","author":"Muschietti","year":"2016","journal-title":"Natural Product Communications"},{"key":"10.1016\/j.phytochem.2021.113014_bib33","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1021\/acs.jnatprod.9b01285","article-title":"Natural products as sources of new drugs over the nearly four decades from 01\/1981 to 09\/2019","volume":"83","author":"Newman","year":"2020","journal-title":"J. Nat. Prod."},{"key":"10.1016\/j.phytochem.2021.113014_bib34","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1016\/S0031-9422(00)81289-3","article-title":"Ambrosanolides and secoambrosanolides from Ambrosia tenuifolia","volume":"25","author":"Oberti","year":"1986","journal-title":"Phytochemistry"},{"key":"10.1016\/j.phytochem.2021.113014_bib35","doi-asserted-by":"crossref","first-page":"2238","DOI":"10.1080\/14786419.2016.1149709","article-title":"Xanthane sesquiterpenoids from the roots and flowers of Xanthium cavanillesii","volume":"30","author":"Olivaro","year":"2016","journal-title":"Nat. Prod. Res."},{"key":"10.1016\/j.phytochem.2021.113014_bib36","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/S0140-6736(17)31612-4","article-title":"Chagas disease","volume":"391","author":"P\u00e9rez-Molina","year":"2018","journal-title":"Lancet"},{"key":"10.1016\/j.phytochem.2021.113014_bib37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2165\/00126839-200304010-00001","article-title":"Immunosuppressive and anti-inflammatory mechanisms of triptolide, the principal active diterpenoid from the Chinese medicinal herb Tripterygium wilfordii hook","volume":"4","author":"Qiu","year":"2003","journal-title":"f. Drugs in R & D"},{"key":"10.1016\/j.phytochem.2021.113014_bib38","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1021\/ar100106n","article-title":"Chemically engineered extracts: source of bioactive compounds","volume":"44","author":"Ramallo","year":"2011","journal-title":"Accounts Chem. Res."},{"key":"10.1016\/j.phytochem.2021.113014_bib39","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1021\/acscombsci.7b00172","article-title":"A bioactive Trypanosoma cruzi bromodomain inhibitor from chemically engineered extracts","volume":"20","author":"Ramallo","year":"2018","journal-title":"ACS Comb. Sci."},{"key":"10.1016\/j.phytochem.2021.113014_bib40","doi-asserted-by":"crossref","first-page":"2397","DOI":"10.2174\/0929867323666160510123255","article-title":"Development of anticancer agents from plant-derived sesquiterpene lactones","volume":"23","author":"Ren","year":"2016","journal-title":"Curr. Med. Chem."},{"key":"10.1016\/j.phytochem.2021.113014_bib41","first-page":"7","article-title":"Current trends in the pharmacological management of Chagas disease","volume":"12","author":"Ribeiro","year":"2020","journal-title":"Int. J. Parasitol.: Drugs and Drug Resistance"},{"key":"10.1016\/j.phytochem.2021.113014_bib42","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1038\/nchem.2479","article-title":"Counting on natural products for drug design","volume":"8","author":"Rodrigues","year":"2016","journal-title":"Nat. Chem."},{"key":"10.1016\/j.phytochem.2021.113014_bib43","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1016\/0031-9422(92)80029-E","article-title":"Sesquiterpene lactones and other constituents of argentine Ambrosia species","volume":"31","author":"Silva","year":"1992","journal-title":"Phytochemistry"},{"key":"10.1016\/j.phytochem.2021.113014_bib44","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0005307","article-title":"GRAIL and otubain-1 are related to T cell hyporesponsiveness during Trypanosoma cruzi infection","volume":"11","author":"Stempin","year":"2017","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.phytochem.2021.113014_bib45","doi-asserted-by":"crossref","first-page":"2415","DOI":"10.1128\/AAC.01630-07","article-title":"Trypanocidal and leishmanicidal activities of sesquiterpene lactones from Ambrosia tenuifolia Sprengel (Asteraceae)","volume":"52","author":"S\u00fclsen","year":"2008","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.phytochem.2021.113014_bib46","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1016\/j.ijantimicag.2011.02.003","article-title":"Psilostachyin C: a natural compound with trypanocidal activity","volume":"37","author":"S\u00fclsen","year":"2011","journal-title":"Int. J. Antimicrob. Agents"},{"key":"10.1016\/j.phytochem.2021.113014_bib47","doi-asserted-by":"crossref","first-page":"e2494","DOI":"10.1371\/journal.pntd.0002494","article-title":"Natural terpenoids from Ambrosia species are active in vitro and in vivo against human pathogenic trypanosomatids","volume":"7","author":"S\u00fclsen","year":"2013","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.phytochem.2021.113014_bib48","article-title":"Mode of action of the sesquiterpene lactones psilostachyin and psilostachyin C on","volume":"11","author":"S\u00fclsen","year":"2016","journal-title":"Trypanosoma cruzi. PLOS ONE"},{"year":"2018","series-title":"Sesquiterpene Lactones Advances in Their Chemistry and Biological Aspects","author":"S\u00fclsen","key":"10.1016\/j.phytochem.2021.113014_bib49"},{"key":"10.1016\/j.phytochem.2021.113014_bib50","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1016\/j.tetlet.2016.01.054","article-title":"Direct chemical derivatization of natural plant extract: straightforward synthesis of natural plant-like hydantoin","volume":"57","author":"Tomohara","year":"2016","journal-title":"Tetrahedron Lett."},{"year":"2021","series-title":"Chagas Disease (American Trypanosomiasis)","author":"World Health Organization","key":"10.1016\/j.phytochem.2021.113014_bib51"},{"key":"10.1016\/j.phytochem.2021.113014_bib52","doi-asserted-by":"crossref","first-page":"1818","DOI":"10.1021\/jo001593z","article-title":"Enantioselective synthesis and stereoselective rearrangements of enol ester epoxides","volume":"66","author":"Zhu","year":"2001","journal-title":"J. Org. Chem."}],"container-title":["Phytochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0031942221003630?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0031942221003630?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T18:45:15Z","timestamp":1758480315000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0031942221003630"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2]]},"references-count":52,"alternative-id":["S0031942221003630"],"URL":"https:\/\/doi.org\/10.1016\/j.phytochem.2021.113014","relation":{},"ISSN":["0031-9422"],"issn-type":[{"type":"print","value":"0031-9422"}],"subject":[],"published":{"date-parts":[[2022,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Psilostachyins as trypanocidal compounds: Bioguided fractionation of Ambrosia tenuifolia chemically modified extract","name":"articletitle","label":"Article Title"},{"value":"Phytochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.phytochem.2021.113014","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"113014"}}