{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T01:04:37Z","timestamp":1768439077832,"version":"3.49.0"},"reference-count":42,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100016179","name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Biodiversidade e Produtos Naturais","doi-asserted-by":"publisher","award":["465637\/2014-0"],"award-info":[{"award-number":["465637\/2014-0"]}],"id":[{"id":"10.13039\/501100016179","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["406119\/2021-0"],"award-info":[{"award-number":["406119\/2021-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["140562\/2019\\u20132"],"award-info":[{"award-number":["140562\/2019\\u20132"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["310183\/2020-0"],"award-info":[{"award-number":["310183\/2020-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100022851","name":"Instituto Nacional de Ci\u00eancia e Tecnologia de Bioanal\u00edtica","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100022851","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Phytochemistry"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.phytochem.2025.114549","type":"journal-article","created":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T12:00:34Z","timestamp":1748001634000},"page":"114549","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Bioactive withanolides from the leaves of Athenaea velutina (Sendtn.) D'Arcy"],"prefix":"10.1016","volume":"238","author":[{"given":"K\u00e9sya Amanda","family":"Dantas Rocha","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edilberto Rocha","family":"Silveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T\u00e9rcio","family":"de Freitas Paulo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9247-6780","authenticated-orcid":false,"given":"Alejandro Pedro","family":"Ayala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno Marques","family":"Soares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Sant\u2019Anna Maranh\u00e3o","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e1udia do \u00d3","family":"Pessoa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8678-3664","authenticated-orcid":false,"given":"Iury Ara\u00fajo","family":"Paz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Lidia","family":"Barroso Rodrigues","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nilberto Robson","family":"Falc\u00e3o do Nascimento","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kirley Marques","family":"Canuto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1617-7586","authenticated-orcid":false,"given":"Ot\u00edlia Deusdenia","family":"Loiola Pessoa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.phytochem.2025.114549_bib1","first-page":"1","article-title":"Withanolides of Athenaea velutina with potential inhibitory properties against SARS coronavirus main protease (mpro): molecular modeling studies","author":"Abreu Alves","year":"2023","journal-title":"J. Biomol. Struct. Dyn."},{"key":"10.1016\/j.phytochem.2025.114549_bib2","doi-asserted-by":"crossref","first-page":"6304","DOI":"10.1080\/14786419.2022.2039135","article-title":"Withalutin, a new cytotoxic withanolide from Athenaea velutina (Sendtn.) D'Arcy","volume":"36","author":"Almeida","year":"2022","journal-title":"Nat. Prod. Res."},{"key":"10.1016\/j.phytochem.2025.114549_bib3","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1055\/a-1395-9046","article-title":"A Withanolide-rich fraction of Athenaea velutina induces apoptosis and cell cycle arrest in melanoma B16F10 cells","volume":"88","author":"Almeida","year":"2022","journal-title":"Planta Med."},{"key":"10.1016\/j.phytochem.2025.114549_bib4","doi-asserted-by":"crossref","DOI":"10.1016\/j.arabjc.2021.103529","article-title":"Anti-amnesic effects of withaferin A, a steroidal lactone isolated from Withania adpressa, on scopolamine-induced memory impairment in mice","volume":"15","author":"Ben Bakrim","year":"2022","journal-title":"Arab. J. Chem."},{"key":"10.1016\/j.phytochem.2025.114549_bib5","series-title":"SAINT Data Reduction Software","year":"2019"},{"key":"10.1016\/j.phytochem.2025.114549_bib6","series-title":"APEX4 Data Collection Software","year":"2021"},{"key":"10.1016\/j.phytochem.2025.114549_bib7","doi-asserted-by":"crossref","first-page":"1338","DOI":"10.1021\/acs.jnatprod.9b00117","article-title":"Selective antiproliferative withanolides from species in the genera Eriolarynx and Deprea","volume":"82","author":"Castro","year":"2019","journal-title":"J. Nat. Prod."},{"key":"10.1016\/j.phytochem.2025.114549_bib8","doi-asserted-by":"crossref","first-page":"2340","DOI":"10.1021\/acs.jnatprod.2c00521","article-title":"The natural products withaferin A and withanone from the medicinal herb Withania somnifera are covalent inhibitors of the SARS-CoV-2 main protease","volume":"85","author":"Chakraborty","year":"2022","journal-title":"J. Nat. Prod."},{"key":"10.1016\/j.phytochem.2025.114549_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/j.phytochem.2022.113338","article-title":"Anti-inflammatory withajardins from the leaves of Athenaea velutina","volume":"203","author":"Dantas Rocha","year":"2022","journal-title":"Phytochemistry"},{"key":"10.1016\/j.phytochem.2025.114549_bib10","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1107\/S0021889808042726","article-title":"OLEX2: a complete structure solution, refinement and analysis program","volume":"42","author":"Dolomanov","year":"2009","journal-title":"J. Appl. Crystallogr."},{"key":"10.1016\/j.phytochem.2025.114549_bib11","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1039\/C9MD00296K","article-title":"Broad-spectrum antitumor properties of withaferin A: a proteomic perspective","volume":"11","author":"Dom","year":"2020","journal-title":"RSC Med. Chem."},{"key":"10.1016\/j.phytochem.2025.114549_bib12","series-title":"Gaussian 09, Gaussian Inc.","author":"Frisch","year":"2009-2021"},{"key":"10.1016\/j.phytochem.2025.114549_bib13","first-page":"1726","article-title":"Withaferin A prevents myocardial ischemia\/reperfusion injury by upregulating amp-activated protein kinase-dependent B-cell lymphoma2 Signaling","volume":"83","author":"Guo","year":"2019","journal-title":"Circ. J. Off. J. Jpn. Circ. Soc."},{"key":"10.1016\/j.phytochem.2025.114549_bib14","doi-asserted-by":"crossref","first-page":"249","DOI":"10.2337\/db21-0470","article-title":"Withaferin A promotes white adipose browning and prevents obesity through sympathetic nerve\u2013activated Prdm16-FATP1 Axis","volume":"71","author":"Guo","year":"2021","journal-title":"Diabetes"},{"key":"10.1016\/j.phytochem.2025.114549_bib15","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1021\/np500824f","article-title":"Withanolides with antibacterial activity from Nicandra john-tyleriana","volume":"78","author":"Guti\u00e9rrez Nicol\u00e1s","year":"2015","journal-title":"J. Nat. Prod."},{"key":"10.1016\/j.phytochem.2025.114549_bib16","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1021\/acs.jnatprod.8b00665","article-title":"Cytotoxic withanolides from the roots of indian ginseng (Withania somnifera)","volume":"82","author":"Kim","year":"2019","journal-title":"J. Nat. Prod."},{"key":"10.1016\/j.phytochem.2025.114549_bib17","doi-asserted-by":"crossref","first-page":"193","DOI":"10.3724\/SP.J.1009.2013.00193","article-title":"Determination of the absolute configuration of natural products","volume":"11","author":"Kong","year":"2013","journal-title":"Chin. J. Nat. Med."},{"key":"10.1016\/j.phytochem.2025.114549_bib18","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1107\/S1600576714022985","article-title":"Comparison of silver and molybdenum microfocus X-ray sources for single-crystal structure determination","volume":"48","author":"Krause","year":"2015","journal-title":"J. Appl. Crystallogr."},{"key":"10.1016\/j.phytochem.2025.114549_bib19","doi-asserted-by":"crossref","first-page":"1646","DOI":"10.1248\/cpb.47.1646","article-title":"Cell differentiation inducing steroids from Withania somnifera L. (DUN.)","volume":"47","author":"Kuroyanagi","year":"1999","journal-title":"Chem. Pharm. Bull. (Tokyo)"},{"key":"10.1016\/j.phytochem.2025.114549_bib20","doi-asserted-by":"crossref","first-page":"3160","DOI":"10.3390\/molecules23123160","article-title":"Leishmanicidal activity of withanolides from Aureliana fasciculata var. fasciculata","volume":"23","author":"Lima","year":"2018","journal-title":"Molecules"},{"key":"10.1016\/j.phytochem.2025.114549_bib21","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1016\/j.ejmech.2012.05.032","article-title":"Withaferin A-related steroids from Withania aristata exhibit potent antiproliferative activity by inducing apoptosis in human tumor cells","volume":"54","author":"Llanos","year":"2012","journal-title":"Eur. J. Med. Chem."},{"key":"10.1016\/j.phytochem.2025.114549_bib22","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.ejmech.2017.09.004","article-title":"Structure-based design, synthesis, and biological evaluation of withaferin A-analogues as potent apoptotic inducers","volume":"140","author":"Llanos","year":"2017","journal-title":"Eur. J. Med. Chem."},{"key":"10.1016\/j.phytochem.2025.114549_bib23","series-title":"Progress in the Chemistry of Organic Natural Products Vol. 94, Fortschritte Der Chemie Organischer Naturstoffe\/Progress in the Chemistry of Organic Natural Products","first-page":"127","article-title":"Withanolides and related steroids","author":"Misico","year":"2011"},{"key":"10.1016\/j.phytochem.2025.114549_bib24","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.phytochem.2025.114549_bib25","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1111\/j.1476-5381.1992.tb09041.x","article-title":"Positive inotropic and negative chronotropic effects of (-)-cis-diltiazem in rat isolated atria","volume":"105","author":"Nasa","year":"1992","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.phytochem.2025.114549_bib27","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1002\/jcc.20823","article-title":"cclib: a library for package-independent computational chemistry algorithms","volume":"29","author":"O\u2019boyle","year":"2008","journal-title":"J. Comput. Chem."},{"key":"10.1016\/j.phytochem.2025.114549_bib28","series-title":"Assay Guiadance Manual. Bethesda: Eli Lilly & Company and the National Center for Advancing Translational Sciences","article-title":"Cell viability assays","author":"Riss","year":"2016"},{"key":"10.1016\/j.phytochem.2025.114549_bib29","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1002\/tax.12089","article-title":"The nomenclatural re-establishment of Athenaea Sendtn. (Solanaceae) with a nomenclatural synopsis of the genus","volume":"68","author":"Rodrigues","year":"2019","journal-title":"Taxon"},{"key":"10.1016\/j.phytochem.2025.114549_bib30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3897\/phytokeys.178.64609","article-title":"Two new species of Athenaea Sendtn. (Solanaceae) from the Atlantic forests of south-eastern Brazil","volume":"178","author":"Rodrigues","year":"2021","journal-title":"PhytoKeys"},{"key":"10.1016\/j.phytochem.2025.114549_bib31","doi-asserted-by":"crossref","first-page":"5904","DOI":"10.1080\/14786419.2020.1802271","article-title":"Antibacterial screening of plants from the Brazilian Atlantic Forest led to the identification of active compounds in Miconia latecrenata (DC.) Naudin","volume":"35","author":"Rodrigues","year":"2021","journal-title":"Nat. Prod. Res."},{"key":"10.1016\/j.phytochem.2025.114549_bib32","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1107\/S2053273314026370","article-title":"SHELXT \u2013 integrated space-group and crystal-structure determination","volume":"71","author":"Sheldrick","year":"2015","journal-title":"Acta Crystallogr. Sect. Found. Adv."},{"key":"10.1016\/j.phytochem.2025.114549_bib33","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1107\/S2053229614024218","article-title":"Crystal structure refinement with SHELXL","volume":"71","author":"Sheldrick","year":"2015","journal-title":"Acta Crystallogr., Sect. C: Struct. Chem."},{"key":"10.1016\/j.phytochem.2025.114549_bib34","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1186\/s13048-020-00684-x","article-title":"Withaferin A: a potential therapeutic agent against COVID-19 infection","volume":"13","author":"Straughn","year":"2020","journal-title":"J. Ovarian Res."},{"key":"10.1016\/j.phytochem.2025.114549_bib35","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1021\/acs.jnatprod.7b00918","article-title":"Withaferin A and withanolide D analogues with dual heat-shock-inducing and cytotoxic activities: semisynthesis and biological evaluation","volume":"81","author":"Wijeratne","year":"2018","journal-title":"J. Nat. Prod."},{"key":"10.1016\/j.phytochem.2025.114549_bib36","doi-asserted-by":"crossref","first-page":"1833","DOI":"10.21873\/anticanres.15659","article-title":"N-Trans-p-coumaroyltyramine enhances indomethacin- and diclofenac-induced apoptosis through endoplasmic reticulum stress-dependent mechanism in MCF-7 cells","volume":"42","author":"Wongsakul","year":"2022","journal-title":"Anticancer Res."},{"key":"10.1016\/j.phytochem.2025.114549_bib37","doi-asserted-by":"crossref","first-page":"3039","DOI":"10.1021\/acs.jmedchem.7b00069","article-title":"17\u03b2-Hydroxywithanolides as sensitizers of renal carcinoma cells to tumor necrosis factor-\u03b1 related apoptosis inducing ligand (TRAIL) mediated apoptosis: structure\u2013activity relationships","volume":"60","author":"Xu","year":"2017","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.phytochem.2025.114549_bib38","doi-asserted-by":"crossref","first-page":"6984","DOI":"10.1021\/acs.jmedchem.5b00867","article-title":"Discovery of Potent 17\u03b2-Hydroxywithanolides for castration-resistant prostate cancer by high-throughput screening of a natural products library for androgen-induced gene expression inhibitors","volume":"58","author":"Xu","year":"2015","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.phytochem.2025.114549_bib39","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1007\/s11101-015-9420-6","article-title":"Phytochemistry and biosynthesis of \u03b4-lactone withanolides","volume":"15","author":"Yang","year":"2016","journal-title":"Phytochem. Rev."},{"key":"10.1016\/j.phytochem.2025.114549_bib40","doi-asserted-by":"crossref","first-page":"3541","DOI":"10.1016\/j.bmcl.2015.06.081","article-title":"Hedgehog inhibitors from Withania somnifera","volume":"25","author":"Yoneyama","year":"2015","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"10.1016\/j.phytochem.2025.114549_bib41","doi-asserted-by":"crossref","first-page":"2532","DOI":"10.1021\/np200635r","article-title":"Cytotoxic withanolide constituents of Physalis longifolia","volume":"74","author":"Zhang","year":"2011","journal-title":"J. Nat. Prod."},{"key":"10.1016\/j.phytochem.2025.114549_bib42","doi-asserted-by":"crossref","first-page":"22819","DOI":"10.1039\/D0RA04106H","article-title":"New withanolides from Physalis minima and their cytotoxicity against A375 human melanoma cells","volume":"10","author":"Zhang","year":"2020","journal-title":"RSC Adv."},{"key":"10.1016\/j.phytochem.2025.114549_bib43","doi-asserted-by":"crossref","DOI":"10.1016\/j.bmcl.2021.128235","article-title":"Vomifoliol isolated from mangrove plant Ceriops tagal inhibits the NFAT signaling pathway with CN as the target enzyme in vitro","volume":"48","author":"Zhang","year":"2021","journal-title":"Bioorg. Med. Chem. Lett."}],"container-title":["Phytochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0031942225001724?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0031942225001724?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:48:05Z","timestamp":1762217285000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0031942225001724"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":42,"alternative-id":["S0031942225001724"],"URL":"https:\/\/doi.org\/10.1016\/j.phytochem.2025.114549","relation":{},"ISSN":["0031-9422"],"issn-type":[{"value":"0031-9422","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Bioactive withanolides from the leaves of Athenaea velutina (Sendtn.) D'Arcy","name":"articletitle","label":"Article Title"},{"value":"Phytochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.phytochem.2025.114549","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"114549"}}