{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T08:32:20Z","timestamp":1786696340263,"version":"build-2736575974"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002784","name":"Canada Excellence Research Chairs","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002784","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Phytochemistry"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.phytochem.2026.115017","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T15:42:55Z","timestamp":1783352575000},"page":"115017","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Molecular cloning and functional characterization of tyrosine decarboxylase genes from galanthamine-producing Narcissus pseudonarcissus \u2018King Alfred.\u2019"],"prefix":"10.1016","volume":"251","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-8963-2261","authenticated-orcid":false,"given":"Arghavan","family":"Arjmandi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bharat Bhusan","family":"Majhi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6235-6465","authenticated-orcid":false,"given":"Natacha","family":"M\u00e9rindol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah-Eve","family":"G\u00e9linas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3270-3133","authenticated-orcid":false,"given":"Karen Cristine Gon\u00e7alves","family":"dos Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0980-9061","authenticated-orcid":false,"given":"Isabella","family":"Burgos-Holguin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6500-3092","authenticated-orcid":false,"given":"Yasmin Moreto","family":"Guaitolini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5236-4979","authenticated-orcid":false,"given":"Patrick","family":"Lag\u00fce","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4355-5503","authenticated-orcid":false,"given":"Isabel","family":"Desgagn\u00e9-Penix","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.phytochem.2026.115017_bib1","doi-asserted-by":"crossref","first-page":"W530","DOI":"10.1093\/nar\/gkab294","article-title":"Plip 2021: expanding the scope of the protein\u2013ligand interaction profiler to DNA and RNA","volume":"49","author":"Adasme","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.phytochem.2026.115017_bib2","first-page":"1","article-title":"An update of the angiosperm phylogeny group classification for the orders and families of flowering plants","volume":"181","author":"Byng","year":"2016","journal-title":"Bot. J. Linn. Soc."},{"key":"10.1016\/j.phytochem.2026.115017_bib3","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.jpba.2014.09.022","article-title":"Alkaloid metabolite profiles by GC\/MS and acetylcholinesterase inhibitory activities with binding-mode predictions of five Amaryllidaceae plants","volume":"102","author":"Cortes","year":"2015","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.phytochem.2026.115017_bib4","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1007\/s11101-020-09678-5","article-title":"Biosynthesis of alkaloids in Amaryllidaceae plants: a review","volume":"20","author":"Desgagn\u00e9-Penix","year":"2021","journal-title":"Phytochem. Rev."},{"key":"10.1016\/j.phytochem.2026.115017_bib5","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/BF01569997","article-title":"Review: optimizing inducer and culture conditions for expression of foreign proteins under the control of the lac promoter","volume":"16","author":"Donovan","year":"1996","journal-title":"J. Ind. Microbiol."},{"key":"10.1016\/j.phytochem.2026.115017_bib7","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1016\/0031-9422(94)00814-A","article-title":"Expression in Escherichia coli and partial characterization of two tyrosine\/dopa decarboxylases from opium poppy","volume":"38","author":"Facchini","year":"1994","journal-title":"Phytochemistry"},{"key":"10.1016\/j.phytochem.2026.115017_bib6","doi-asserted-by":"crossref","first-page":"26684","DOI":"10.1016\/S0021-9258(18)47073-1","article-title":"Differential and tissue-specific expression of a gene family for tyrosine\/dopa decarboxylase in opium poppy","volume":"269","author":"Facchini","year":"1994","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.phytochem.2026.115017_bib8","doi-asserted-by":"crossref","DOI":"10.3390\/molecules25204670","article-title":"Recent progress in Amaryllidaceae biotechnology","volume":"25","author":"Georgiev","year":"2020","journal-title":"Molecules"},{"key":"10.1016\/j.phytochem.2026.115017_bib9","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/0147-619X(80)90110-9","article-title":"The functional organization of the Nopaline A. tumerfaciens plasmid pTiC58","volume":"3","author":"Holsters","year":"1980","journal-title":"Plasmid"},{"key":"10.1016\/j.phytochem.2026.115017_bib10","doi-asserted-by":"crossref","DOI":"10.3390\/genes10080594","article-title":"Developmental regulation of the expression of Amaryllidaceae alkaloid biosynthetic genes in Narcissus papyraceus","volume":"10","author":"Hotchandani","year":"2019","journal-title":"Genes"},{"key":"10.1016\/j.phytochem.2026.115017_bib11","doi-asserted-by":"crossref","DOI":"10.1007\/s11738-021-03258-6","article-title":"Molecular cloning and functional characterization of tyrosine decarboxylases from galanthamine-producing Lycoris radiata","volume":"43","author":"Hu","year":"2021","journal-title":"Acta Physiol. Plant."},{"key":"10.1016\/j.phytochem.2026.115017_bib12","series-title":"Gt: Easily Create Presentation-Ready Display Tables","author":"Iannone","year":"2025"},{"key":"10.1016\/j.phytochem.2026.115017_bib13","doi-asserted-by":"crossref","first-page":"6065","DOI":"10.1021\/acs.jcim.0c00675","article-title":"ZINC20-A free ultralarge-scale chemical database for ligand discovery","volume":"60","author":"Irwin","year":"2020","journal-title":"J. Chem. Inf. Model."},{"key":"10.1016\/j.phytochem.2026.115017_bib14","doi-asserted-by":"crossref","DOI":"10.1128\/AAC.00398-21","article-title":"Amaryllidaceae alkaloid cherylline inhibits the replication of dengue and zika viruses","volume":"65","author":"Ka","year":"2021","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.phytochem.2026.115017_bib15","doi-asserted-by":"crossref","first-page":"2189","DOI":"10.1016\/S0021-9258(18)53980-6","article-title":"A pathogen-responsive gene of parsley encodes tyrosine decarboxylase","volume":"268","author":"Kawalleck","year":"1993","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.phytochem.2026.115017_bib16","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0103223","article-title":"Cloning and characterization of a norbelladine 4\u2032-O-Methyltransferase involved in the biosynthesis of the Alzheimer's drug galanthamine in Narcissus sp. aff. pseudonarcissus","volume":"9","author":"Kilgore","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.phytochem.2026.115017_bib17","doi-asserted-by":"crossref","first-page":"16740","DOI":"10.1074\/jbc.M116.717827","article-title":"Identification of a noroxomaritidine reductase with Amaryllidaceae alkaloid biosynthesis related activities","volume":"291","author":"Kilgore","year":"2016","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.phytochem.2026.115017_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2024.107649","article-title":"Kinetic and in silico structural characterization of norbelladine O-methyltransferase of Amaryllidaceae alkaloids biosynthesis","volume":"300","author":"Koirala","year":"2024","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.phytochem.2026.115017_bib19","doi-asserted-by":"crossref","first-page":"6623","DOI":"10.1074\/jbc.M109.060640","article-title":"Identification and biochemical characterization of molybdenum cofactor-binding proteins from Arabidopsis thaliana","volume":"285","author":"Kruse","year":"2010","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.phytochem.2026.115017_bib20","doi-asserted-by":"crossref","first-page":"1988","DOI":"10.1111\/pbi.70026","article-title":"Elucidating the enzyme network driving Amaryllidaceae alkaloids biosynthesis in Leucojum aestivum","volume":"23","author":"Lamichhane","year":"2025","journal-title":"Plant Biotechnol. J."},{"key":"10.1016\/j.phytochem.2026.115017_bib21","doi-asserted-by":"crossref","first-page":"1895","DOI":"10.1016\/j.febslet.2009.05.017","article-title":"Gene expression and characterization of a stress-induced tyrosine decarboxylase from Arabidopsis thaliana","volume":"583","author":"Lehmann","year":"2009","journal-title":"FEBS Lett."},{"key":"10.1016\/j.phytochem.2026.115017_bib22","doi-asserted-by":"crossref","first-page":"W78","DOI":"10.1093\/nar\/gkae268","article-title":"Interactive tree of life (iTOL) v6: recent updates to the phylogenetic tree display and annotation tool","volume":"52","author":"Letunic","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.phytochem.2026.115017_bib24","doi-asserted-by":"crossref","DOI":"10.1134\/S1021443725602915","article-title":"Physiological and molecular regulation of Amaryllidaceae alkaloid biosynthesis in lycoris species","volume":"72","author":"Li","year":"2025","journal-title":"Russ. J. Plant Physiol."},{"key":"10.1016\/j.phytochem.2026.115017_bib23","article-title":"Identification and functional characterization of tyrosine decarboxylase from Rehmannia glutinosa","volume":"27","author":"Li","year":"2022","journal-title":"Molecules"},{"key":"10.1016\/j.phytochem.2026.115017_bib25","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/0005-2744(78)90211-5","article-title":"Specificity and reactivity of human granulocyte elastase and Cathepsin G, porcine pancreatic elastase, bovine chymotrypsin and trypsin toward inhibition with sulfonyl fluorides","volume":"525","author":"Lively","year":"1978","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.phytochem.2026.115017_bib26","doi-asserted-by":"crossref","DOI":"10.3390\/ijms252010892","article-title":"Functional and physiological characterization of tyrosine decarboxylases from Olea europaea L. involved in the synthesis of the main phenolics in olive fruit and virgin olive oil","volume":"25","author":"Luaces","year":"2024","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.phytochem.2026.115017_bib27","doi-asserted-by":"crossref","DOI":"10.3389\/fpls.2023.1231809","article-title":"Characterization of norbelladine synthase and noroxomaritidine\/norcraugsodine reductase reveals a novel catalytic route for the biosynthesis of Amaryllidaceae alkaloids including the Alzheimer's drug galanthamine","volume":"14","author":"Majhi","year":"2023","journal-title":"Front. Plant Sci."},{"key":"10.1016\/j.phytochem.2026.115017_bib28","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1111\/j.1574-6968.1999.tb08777.x","article-title":"Tyrosine decarboxylase activity of Lactobacillus brevis IOEB 9809 isolated from wine and L. brevis ATCC 367","volume":"180","author":"Moreno-Arribas","year":"1999","journal-title":"FEMS (Fed. Eur. Microbiol. Soc.) Microbiol. Lett."},{"key":"10.1016\/j.phytochem.2026.115017_bib29","doi-asserted-by":"crossref","DOI":"10.3390\/molecules31020258","article-title":"Amaryllidaceae alkaloids and phenolic acids identification in Leucojum aestivum L. plant cultures exposed to different temperature conditions","volume":"31","author":"Ptak","year":"2026","journal-title":"Molecules"},{"key":"10.1016\/j.phytochem.2026.115017_bib30","series-title":"R: a Language and Environment for Statistical Computing","year":"2024"},{"key":"10.1016\/j.phytochem.2026.115017_bib31","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1111\/j.1432-1033.1994.tb18816.x","article-title":"Multiple evolutionary origin of pyridoxal-5'-phosphate-dependent amino acid decarboxylases","volume":"221","author":"Sandmeier","year":"1994","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.phytochem.2026.115017_bib33","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1186\/s12870-018-1570-4","article-title":"Cloning and characterization of norbelladine synthase catalyzing the first committed reaction in Amaryllidaceae alkaloid biosynthesis","volume":"18","author":"Singh","year":"2018","journal-title":"BMC Plant Biol."},{"key":"10.1016\/j.phytochem.2026.115017_bib32","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-017-17724-0","article-title":"Transcriptome and metabolome profiling of Narcissus pseudonarcissus \u2018King Alfred\u2019 reveal components of Amaryllidaceae alkaloid metabolism","volume":"7","author":"Singh","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.phytochem.2026.115017_bib36","doi-asserted-by":"crossref","first-page":"10806","DOI":"10.1073\/pnas.1920097117","article-title":"Structural basis for divergent and convergent evolution of catalytic machineries in plant aromatic amino acid decarboxylase proteins","volume":"117","author":"Torrens-Spence","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.phytochem.2026.115017_bib34","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.bbrc.2011.12.124","article-title":"Biochemical evaluation of a parsley tyrosine decarboxylase results in a novel 4-hydroxyphenylacetaldehyde synthase enzyme","volume":"418","author":"Torrens-Spence","year":"2012","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.phytochem.2026.115017_bib35","doi-asserted-by":"crossref","first-page":"2376","DOI":"10.1074\/jbc.M112.401752","article-title":"Biochemical evaluation of the decarboxylation and decarboxylation-deamination activities of plant aromatic amino acid decarboxylases","volume":"288","author":"Torrens-Spence","year":"2013","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.phytochem.2026.115017_bib37","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1007\/s00425-021-03741-x","article-title":"Transcriptome analysis of Leucojum aestivum and identification of genes involved in norbelladine biosynthesis","volume":"255","author":"Tousignant","year":"2022","journal-title":"Planta"},{"key":"10.1016\/j.phytochem.2026.115017_bib38","article-title":"Cloning and characterization of a tyrosine decarboxylase involved in the biosynthesis of galanthamine in Lycoris aurea","volume":"7","author":"Wang","year":"2019","journal-title":"PeerJ"},{"key":"10.1016\/j.phytochem.2026.115017_bib39","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.pep.2013.10.018","article-title":"Tyrosine decarboxylase from Lactobacillus brevis: soluble expression and characterization","volume":"94","author":"Zhang","year":"2014","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.phytochem.2026.115017_bib40","article-title":"Crystal structure of tyrosine decarboxylase and identification of key residues involved in conformational swing and substrate binding","volume":"6","author":"Zhu","year":"2016","journal-title":"Sci. Rep."}],"container-title":["Phytochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0031942226002505?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0031942226002505?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T08:16:14Z","timestamp":1786695374000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0031942226002505"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":40,"alternative-id":["S0031942226002505"],"URL":"https:\/\/doi.org\/10.1016\/j.phytochem.2026.115017","relation":{},"ISSN":["0031-9422"],"issn-type":[{"value":"0031-9422","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular cloning and functional characterization of tyrosine decarboxylase genes from galanthamine-producing Narcissus pseudonarcissus \u2018King Alfred.\u2019","name":"articletitle","label":"Article Title"},{"value":"Phytochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.phytochem.2026.115017","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"115017"}}