{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T05:01:00Z","timestamp":1789016460760,"version":"build-2803163510"},"reference-count":34,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,11,18]],"date-time":"2020-11-18T00:00:00Z","timestamp":1605657600000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100011912","name":"Washington University School of Medicine in St. Louis","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011912","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Practical Laboratory Medicine"],"published-print":{"date-parts":[[2020,11]]},"DOI":"10.1016\/j.plabm.2020.e00191","type":"journal-article","created":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T21:18:21Z","timestamp":1606252701000},"page":"e00191","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":19,"special_numbering":"C","title":["Validation and interpretation of IGH and TCR clonality testing by Ion Torrent S5 NGS for diagnosis and disease monitoring in B and T cell cancers"],"prefix":"10.1016","volume":"22","author":[{"given":"Lindsay","family":"Lay","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brooke","family":"Stroup","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline E.","family":"Payton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.plabm.2020.e00191_bib1","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1038\/sj.leu.2404467","article-title":"Improved reliability of lymphoma diagnostics via PCR-based clonality testing: \u2014 report of the BIOMED-2 Concerted Action BHM4-CT98-3936","volume":"21","author":"van Krieken","year":"2006","journal-title":"Leukemia"},{"issue":"2","key":"10.1016\/j.plabm.2020.e00191_bib2","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1038\/sj.leu.2404481","article-title":"Powerful strategy for polymerase chain reaction-based clonality assessment in T-cell malignancies Report of the BIOMED-2 Concerted Action BHM4 CT98-3936","volume":"21","author":"Br\u00fcggemann","year":"2007","journal-title":"Leukemia"},{"issue":"2","key":"10.1016\/j.plabm.2020.e00191_bib3","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1038\/sj.leu.2404479","article-title":"Significantly improved PCR-based clonality testing in B-cell malignancies by use of multiple immunoglobulin gene targets. Report of the BIOMED-2 Concerted Action BHM4-CT98-3936","volume":"21","author":"Evans","year":"2007","journal-title":"Leukemia"},{"issue":"12","key":"10.1016\/j.plabm.2020.e00191_bib4","doi-asserted-by":"crossref","first-page":"2257","DOI":"10.1038\/sj.leu.2403202","article-title":"Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98-3936","volume":"17","author":"Dongen JM van","year":"2003","journal-title":"Leukemia"},{"issue":"2","key":"10.1016\/j.plabm.2020.e00191_bib5","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.jmoldx.2018.10.008","article-title":"Establishment of immunoglobulin heavy (IGH) chain clonality testing by next-generation sequencing for routine characterization of B-cell and plasma cell neoplasms","volume":"21","author":"Arcila","year":"2019","journal-title":"J.\u00a0Mol. Diagn. JMD."},{"issue":"9","key":"10.1016\/j.plabm.2020.e00191_bib6","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1136\/jclinpath-2018-205004","article-title":"Role of high-throughput sequencing in the diagnosis of cutaneous T-cell lymphoma","volume":"71","author":"Rea","year":"2018","journal-title":"J.\u00a0Clin. Pathol."},{"issue":"2","key":"10.1016\/j.plabm.2020.e00191_bib7","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.jaad.2015.04.030","article-title":"T-cell clonality assessment by next-generation sequencing improves detection sensitivity in mycosis fungoides","volume":"73","author":"Sufficool","year":"2015","journal-title":"J.\u00a0Am. Acad. Dermatol."},{"issue":"4","key":"10.1016\/j.plabm.2020.e00191_bib8","doi-asserted-by":"crossref","first-page":"331","DOI":"10.3343\/alm.2017.37.4.331","article-title":"Detection of immunoglobulin heavy chain gene clonality by next-generation sequencing for minimal residual disease monitoring in B-lymphoblastic leukemia","volume":"37","author":"Shin","year":"2017","journal-title":"Ann. Lab. Med."},{"key":"10.1016\/j.plabm.2020.e00191_bib9","doi-asserted-by":"crossref","first-page":"449","DOI":"10.3389\/fonc.2019.00449","article-title":"Standardized minimal residual disease detection by next-generation sequencing in multiple myeloma","volume":"9","author":"Yao","year":"2019","journal-title":"Front. Oncol."},{"issue":"3","key":"10.1016\/j.plabm.2020.e00191_bib10","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0211600","article-title":"Baseline identification of clonal V(D)J sequences for DNA-based minimal residual disease detection in multiple myeloma","volume":"14","author":"Rustad","year":"2019","journal-title":"PloS One"},{"issue":"26","key":"10.1016\/j.plabm.2020.e00191_bib11","doi-asserted-by":"crossref","first-page":"5173","DOI":"10.1182\/blood-2012-07-444042","article-title":"Deep-sequencing approach for minimal residual disease detection in acute lymphoblastic leukemia","volume":"120","author":"Faham","year":"2012","journal-title":"Blood"},{"issue":"3","key":"10.1016\/j.plabm.2020.e00191_bib12","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1309\/AJCP5TYGBVW4ZITR","article-title":"A\u00a0comparison of deep sequencing of TCRG rearrangements vs traditional capillary electrophoresis for assessment of clonality in T-Cell lymphoproliferative disorders","volume":"141","author":"Schumacher","year":"2014","journal-title":"Am. J. Clin. Pathol."},{"issue":"9","key":"10.1016\/j.plabm.2020.e00191_bib13","doi-asserted-by":"crossref","first-page":"2227","DOI":"10.1038\/s41375-019-0508-7","article-title":"Next-generation sequencing of immunoglobulin gene rearrangements for clonality assessment: a technical feasibility study by EuroClonality-NGS","volume":"33","author":"Scheijen","year":"2019","journal-title":"Leukemia"},{"issue":"9","key":"10.1016\/j.plabm.2020.e00191_bib14","doi-asserted-by":"crossref","first-page":"2254","DOI":"10.1038\/s41375-019-0499-4","article-title":"Quality control and quantification in IG\/TR next-generation sequencing marker identification: protocols and bioinformatic functionalities by EuroClonality-NGS","volume":"33","author":"Knecht","year":"2019","journal-title":"Leukemia"},{"issue":"17","key":"10.1016\/j.plabm.2020.e00191_bib15","doi-asserted-by":"crossref","first-page":"4540","DOI":"10.1158\/1078-0432.CCR-13-3231","article-title":"Detection of minimal residual disease in B lymphoblastic leukemia by high-throughput sequencing of IGH","volume":"20","author":"Wu","year":"2014","journal-title":"Clin. Cancer Res. Off. J. Am. Assoc. Cancer Res."},{"issue":"3","key":"10.1016\/j.plabm.2020.e00191_bib16","doi-asserted-by":"crossref","first-page":"178","DOI":"10.18632\/oncotarget.235","article-title":"IgH gene rearrangements as plasma biomarkers in Non- Hodgkin\u2019s lymphoma patients","volume":"2","author":"He","year":"2011","journal-title":"Oncotarget"},{"issue":"8","key":"10.1016\/j.plabm.2020.e00191_bib17","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1182\/blood-2015-03-633685","article-title":"Prognostic significance of minimal residual disease in high risk B-ALL: a report from Children\u2019s Oncology Group study AALL0232","volume":"126","author":"Borowitz","year":"2015","journal-title":"Blood"},{"issue":"12","key":"10.1016\/j.plabm.2020.e00191_bib18","doi-asserted-by":"crossref","first-page":"1350","DOI":"10.1182\/blood-2017-09-806521","article-title":"Measurable residual disease detection by high-throughput sequencing improves risk stratification for pediatric B-ALL","volume":"131","author":"Wood","year":"2018","journal-title":"Blood"},{"issue":"9","key":"10.1016\/j.plabm.2020.e00191_bib19","doi-asserted-by":"crossref","first-page":"2241","DOI":"10.1038\/s41375-019-0496-7","article-title":"Standardized next-generation sequencing of immunoglobulin and T-cell receptor gene recombinations for MRD marker identification in acute lymphoblastic leukaemia; a EuroClonality-NGS validation study","volume":"33","author":"Br\u00fcggemann","year":"2019","journal-title":"Leukemia"},{"issue":"1","key":"10.1016\/j.plabm.2020.e00191_bib20","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1053\/j.seminhematol.2018.05.008","article-title":"Molecular assessment of clonality in lymphoid neoplasms","volume":"56","author":"Wang","year":"2019","journal-title":"Semin. Hematol."},{"issue":"10","key":"10.1016\/j.plabm.2020.e00191_bib21","doi-asserted-by":"crossref","first-page":"3432","DOI":"10.1172\/JCI64101","article-title":"Molecular pathogenesis of chronic lymphocytic leukemia","volume":"122","author":"Gaidano","year":"2012","journal-title":"J.\u00a0Clin. Invest."},{"issue":"23","key":"10.1016\/j.plabm.2020.e00191_bib22","doi-asserted-by":"crossref","first-page":"3506","DOI":"10.1182\/bloodadvances.2017014639","article-title":"CapTCR-seq: hybrid capture for T-cell receptor repertoire profiling","volume":"2","author":"Mulder","year":"2018","journal-title":"Blood Adv"},{"issue":"4","key":"10.1016\/j.plabm.2020.e00191_bib23","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.cmi.2017.10.013","article-title":"Next-generation sequencing technologies and their application to the study and control of bacterial infections","volume":"24","author":"Besser","year":"2018","journal-title":"Clin. Microbiol. Infect."},{"issue":"1","key":"10.1016\/j.plabm.2020.e00191_bib24","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1038\/s41598-017-00510-3","article-title":"Comparison of two massively parallel sequencing platforms using 83 single nucleotide polymorphisms for human identification","volume":"7","author":"Apaga","year":"2017","journal-title":"Sci. Rep."},{"issue":"10","key":"10.1016\/j.plabm.2020.e00191_bib25","doi-asserted-by":"crossref","first-page":"2159","DOI":"10.1038\/leu.2012.246","article-title":"EuroClonality\/BIOMED-2 guidelines for interpretation and reporting of Ig\/TCR clonality testing in suspected lymphoproliferations","volume":"26","author":"Langerak","year":"2012","journal-title":"Leukemia"},{"key":"10.1016\/j.plabm.2020.e00191_bib26","unstructured":"Ion 520TM & Ion 530TM Kit \u2013 OT2 Quick Reference. Pub. No. MAN0010849 Rev. D.0."},{"key":"10.1016\/j.plabm.2020.e00191_bib27","article-title":"Instructions for use LymphoTrack\u00ae TRG assay \u2013 PGM","year":"2017","journal-title":"280330 Rev. H \u2013 Mar"},{"key":"10.1016\/j.plabm.2020.e00191_bib28","article-title":"Instructions for use LymphoTrack\u00ae IGH (FR1, FR2, & FR3) assays \u2013 PGM","year":"2017","journal-title":"280376 Rev. B \u2013 Mar."},{"key":"10.1016\/j.plabm.2020.e00191_bib29","series-title":"Agilent High Sensitivity DNA Kit Guide, G2938-90321","year":"2013"},{"issue":"Suppl 1","key":"10.1016\/j.plabm.2020.e00191_bib30","article-title":"Next generation sequencing (NGS) based IGH and TCR clonality assays provide excellent specificity and sensitivity for routine clonal characterization and monitoring of lymphoproliferative disorders","volume":"130","author":"Arcila","year":"2017","journal-title":"Blood"},{"issue":"6","key":"10.1016\/j.plabm.2020.e00191_bib31","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1056\/NEJMoa1714678","article-title":"Daratumumab plus bortezomib, melphalan, and prednisone for untreated myeloma","volume":"378","author":"Mateos","year":"2018","journal-title":"N.\u00a0Engl. J. Med."},{"issue":"20","key":"10.1016\/j.plabm.2020.e00191_bib32","doi-asserted-by":"crossref","first-page":"3073","DOI":"10.1182\/blood-2014-01-550020","article-title":"Prognostic value of deep sequencing method for minimal residual disease detection in multiple myeloma","volume":"123","author":"Martinez-Lopez","year":"2014","journal-title":"Blood"},{"issue":"5","key":"10.1016\/j.plabm.2020.e00191_bib33","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.1016\/j.jaad.2020.05.110","article-title":"Evaluation of the PCR-based T cell receptor-\u03b2 clonality test in the diagnosis of early mycosis fungoides","volume":"83","author":"Schachter","year":"2020","journal-title":"J.\u00a0Am. Acad. Dermatol."},{"issue":"308","key":"10.1016\/j.plabm.2020.e00191_bib34","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.aaa9122","article-title":"TCR sequencing facilitates diagnosis and identifies mature T cells as the cell of origin in CTCL","volume":"7","author":"Kirsch","year":"2015","journal-title":"Sci. Transl. Med."}],"container-title":["Practical Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352551720301542?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2352551720301542?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T01:15:55Z","timestamp":1762478155000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2352551720301542"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11]]},"references-count":34,"alternative-id":["S2352551720301542"],"URL":"https:\/\/doi.org\/10.1016\/j.plabm.2020.e00191","relation":{},"ISSN":["2352-5517"],"issn-type":[{"value":"2352-5517","type":"print"}],"subject":[],"published":{"date-parts":[[2020,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Validation and interpretation of IGH and TCR clonality testing by Ion Torrent S5 NGS for diagnosis and disease monitoring in B and T cell cancers","name":"articletitle","label":"Article Title"},{"value":"Practical Laboratory Medicine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.plabm.2020.e00191","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"e00191"}}