{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T13:29:45Z","timestamp":1792762185046,"version":"4.5.0"},"reference-count":116,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T00:00:00Z","timestamp":1768694400000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001081","name":"The University of Newcastle Hunter Medical Research Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001081","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Placenta"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1016\/j.placenta.2026.01.009","type":"journal-article","created":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T00:27:29Z","timestamp":1768609649000},"update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"title":["Exploring placental ultrastructure: A review of electron microscopy techniques and emerging methods for resolving 3D organelle architecture"],"prefix":"10.1016","author":[{"given":"Siddharth","family":"Acharya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Hanssen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James C.","family":"Bouwer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John E.","family":"Schjenken","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsty G.","family":"Pringle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0505-0598","authenticated-orcid":false,"given":"Joshua J.","family":"Fisher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.placenta.2026.01.009_bib1","doi-asserted-by":"crossref","DOI":"10.3389\/fphys.2018.01091","article-title":"The role of placental hormones in mediating maternal adaptations to support pregnancy and lactation","volume":"9","author":"Napso","year":"2018","journal-title":"Front. Physiol."},{"issue":"2\u20133","key":"10.1016\/j.placenta.2026.01.009_bib2","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1387\/ijdb.082759ja","article-title":"Developmental cell biology of human villous trophoblast: current research problems","volume":"54","author":"Aplin","year":"2010","journal-title":"Int. J. Dev. Biol."},{"issue":"18","key":"10.1016\/j.placenta.2026.01.009_bib3","doi-asserted-by":"crossref","first-page":"3479","DOI":"10.1007\/s00018-019-03104-6","article-title":"Human placenta and trophoblast development: key molecular mechanisms and model systems","volume":"76","author":"Kn\u00f6fler","year":"2019","journal-title":"Cell. Mol. Life Sci."},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib4","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/0892-0354(91)90019-9","article-title":"Ultrastructure of the normal human placenta","volume":"4","author":"Jones","year":"1991","journal-title":"Electron. Microsc. Rev."},{"issue":"Pt 3","key":"10.1016\/j.placenta.2026.01.009_bib5","first-page":"535","article-title":"Electron microscopic observations on the cytotrophoblast contribution to the syncytium in the human placenta","volume":"100","author":"Boyd","year":"1966","journal-title":"J. Anat."},{"key":"10.1016\/j.placenta.2026.01.009_bib6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.placenta.2019.05.004","article-title":"Mitochondrial isolation, cryopreservation and preliminary biochemical characterisation from placental cytotrophoblast and syncytiotrophoblast","volume":"82","author":"Fisher","year":"2019","journal-title":"Placenta"},{"key":"10.1016\/j.placenta.2026.01.009_bib7","article-title":"Electron tomography reveals mitochondrial network and cristae remodelling during cell differentiation in the human placenta","volume":"2025","author":"Acharya","year":"2025","journal-title":"bioRxiv"},{"issue":"3","key":"10.1016\/j.placenta.2026.01.009_bib8","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1128\/jb.41.3.397-413.1941","article-title":"The electron microscope: a new tool for bacteriological research","volume":"41","author":"Marton","year":"1941","journal-title":"J. Bacteriol."},{"issue":"6297","key":"10.1016\/j.placenta.2026.01.009_bib9","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1126\/science.aaf8589","article-title":"Electron microscopy of electromagnetic waveforms","volume":"353","author":"Ryabov","year":"2016","journal-title":"Science"},{"issue":"4","key":"10.1016\/j.placenta.2026.01.009_bib10","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1063\/1.1683435","article-title":"Electron Gun using a field emission source","volume":"39","author":"Crewe","year":"1968","journal-title":"Rev. Sci. Instrum."},{"key":"10.1016\/j.placenta.2026.01.009_bib11","series-title":"Thermionic Emission, Electron-Emission Gas Discharges I\/Elektronen-Emission Gasentladungen I","first-page":"1","author":"Nottingham","year":"1956"},{"issue":"4","key":"10.1016\/j.placenta.2026.01.009_bib12","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1038\/nmat2380","article-title":"Structure and bonding at the atomic scale by scanning transmission electron microscopy","volume":"8","author":"Muller","year":"2009","journal-title":"Nat. Mater."},{"issue":"2","key":"10.1016\/j.placenta.2026.01.009_bib13","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1111\/jmi.12120","article-title":"Backscattered electron SEM imaging of cells and determination of the information depth","volume":"254","author":"Seiter","year":"2014","journal-title":"J. Microsc."},{"issue":"8","key":"10.1016\/j.placenta.2026.01.009_bib14","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1016\/j.semcdb.2009.07.008","article-title":"Electron tomography in life science","volume":"20","author":"B\u00e1rcena","year":"2009","journal-title":"Semin. Cell Dev. Biol."},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib15","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1038\/s43586-022-00131-9","article-title":"Volume electron microscopy","volume":"2","author":"Peddie","year":"2022","journal-title":"Nat. Rev. Methods Primers"},{"issue":"15","key":"10.1016\/j.placenta.2026.01.009_bib16","doi-asserted-by":"crossref","first-page":"2649","DOI":"10.1016\/j.cell.2022.06.034","article-title":"Cryo-electron tomography: a long journey to the inner space of cells","volume":"185","author":"Baumeister","year":"2022","journal-title":"Cell"},{"issue":"17","key":"10.1016\/j.placenta.2026.01.009_bib17","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2421538122","article-title":"Reducing the effects of radiation damage in cryo-EM using liquid helium temperatures","volume":"122","author":"Dickerson","year":"2025","journal-title":"Proc. Natl. Acad. Sci."},{"issue":"3","key":"10.1016\/j.placenta.2026.01.009_bib18","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1038\/nmeth1014","article-title":"Focused-ion-beam thinning of frozen-hydrated biological specimens for cryo-electron microscopy","volume":"4","author":"Marko","year":"2007","journal-title":"Nat. Methods"},{"issue":"3","key":"10.1016\/j.placenta.2026.01.009_bib19","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1002\/aja.1000930303","article-title":"Electron microscopy of the visceral yolksac epithelium of the Guinea pig","volume":"93","author":"Dempsey","year":"1953","journal-title":"Am. J. Anat."},{"key":"10.1016\/j.placenta.2026.01.009_bib20","first-page":"609","article-title":"Electron microscopy of human placental villi","volume":"117","author":"Dempsey","year":"1953","journal-title":"Anat. Rec."},{"issue":"2","key":"10.1016\/j.placenta.2026.01.009_bib21","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1002\/ar.1091230202","article-title":"Electron microscopy of the human placenta","volume":"123","author":"Wislocki","year":"1955","journal-title":"Anat. Rec."},{"issue":"3","key":"10.1016\/j.placenta.2026.01.009_bib22","first-page":"356","article-title":"Observations on human chorionic villi using the electron microscope","volume":"88","author":"Boyd","year":"1954","journal-title":"J. Anat."},{"issue":"6678","key":"10.1016\/j.placenta.2026.01.009_bib23","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1038\/33823","article-title":"Electron microscopy image enhanced","volume":"392","author":"Haider","year":"1998","journal-title":"Nature"},{"issue":"5","key":"10.1016\/j.placenta.2026.01.009_bib24","first-page":"294","article-title":"Development of an ultrahigh vacuum high resolution scanning transmission electron microscope","volume":"42","author":"Harada","year":"1993","journal-title":"J. Electron. Microsc."},{"issue":"4","key":"10.1016\/j.placenta.2026.01.009_bib25","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1063\/1.1699652","article-title":"A lapping technique: to improve the image quality of electron microscope lenses","volume":"21","author":"Hamm","year":"1950","journal-title":"J. Appl. Phys."},{"issue":"3","key":"10.1016\/j.placenta.2026.01.009_bib26","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/j.jsb.2011.05.012","article-title":"Practical performance evaluation of a 10k\u00d710k CCD for electron cryo-microscopy","volume":"175","author":"Bammes","year":"2011","journal-title":"J. Struct. Biol."},{"issue":"2","key":"10.1016\/j.placenta.2026.01.009_bib27","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1364\/JOSA.57.000232","article-title":"Image recording in Electron Microscopy","volume":"57","author":"Hamilton","year":"1967","journal-title":"J. Opt. Soc. Am."},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib28","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/0304-3991(92)90027-H","article-title":"The photographic emulsion as analog recorder for electrons","volume":"46","author":"Zeitler","year":"1992","journal-title":"Ultramicroscopy"},{"issue":"2","key":"10.1016\/j.placenta.2026.01.009_bib29","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1515\/mir-2012-0008","article-title":"The role of microfilm in digital preservation","volume":"41","author":"Brown","year":"2012","journal-title":"Microform & Digitization Rev."},{"issue":"3","key":"10.1016\/j.placenta.2026.01.009_bib30","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1016\/j.jsb.2006.08.019","article-title":"Assessing the capabilities of a 4kx4k CCD camera for electron cryo-microscopy at 300kV","volume":"156","author":"Booth","year":"2006","journal-title":"J. Struct. Biol."},{"issue":"Pt 3","key":"10.1016\/j.placenta.2026.01.009_bib31","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1107\/S2059798324000986","article-title":"A service-based approach to cryoEM facility processing pipelines at eBIC","volume":"80","author":"Horstmann","year":"2024","journal-title":"Acta Crystallogr D Struct Biol"},{"issue":"4","key":"10.1016\/j.placenta.2026.01.009_bib32","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1083\/jcb.2.4.305","article-title":"Variations in the structure of mitochondria","volume":"2","author":"Dempsey","year":"1956","journal-title":"J. Biophys. Biochem. Cytol."},{"issue":"3","key":"10.1016\/j.placenta.2026.01.009_bib33","article-title":"Electronmicroscopic observation on the chorionic villi of the normal human placenta and chorioepithelioma maligunum, 1","volume":"8","author":"Wakitani","year":"1961","journal-title":"J. Jpn. Obstet. Gynecol. Soc."},{"issue":"Pt 3","key":"10.1016\/j.placenta.2026.01.009_bib34","first-page":"297","article-title":"Development of the human placenta in the first three months of gestation","volume":"94","author":"Hamilton","year":"1960","journal-title":"J. Anat."},{"issue":"3","key":"10.1016\/j.placenta.2026.01.009_bib35","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/S0143-4004(82)80004-0","article-title":"A three-dimensional study of the normal human placental villous core: II. Stromal architecture","volume":"3","author":"Castellucci","year":"1982","journal-title":"Placenta"},{"issue":"4","key":"10.1016\/j.placenta.2026.01.009_bib36","first-page":"1811","article-title":"The fine structure of the human placental villus as revealed by scanning electron microscopy","volume":"1","author":"Burton","year":"1987","journal-title":"Scanning Microsc."},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib37","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1002\/ar.1091750104","article-title":"Multivesicular bodies and related structures of the syncytiotrophoblast of human term placenta","volume":"175","author":"Martin","year":"1973","journal-title":"Anat. Rec."},{"issue":"5","key":"10.1016\/j.placenta.2026.01.009_bib38","doi-asserted-by":"crossref","first-page":"2172","DOI":"10.1210\/endo.138.5.5133","article-title":"Structural and functional changes in Mitochondria associated with trophoblast differentiation: methods to isolate enriched preparations of Syncytiotrophoblast Mitochondria","volume":"138","author":"Mart\u00ednez","year":"1997","journal-title":"Endocrinology"},{"issue":"9","key":"10.1016\/j.placenta.2026.01.009_bib39","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1111\/cmi.12132","article-title":"Electron tomography of Plasmodium falciparum merozoites reveals core cellular events that underpin erythrocyte invasion","volume":"15","author":"Hanssen","year":"2013","journal-title":"Cell. Microbiol."},{"issue":"3","key":"10.1016\/j.placenta.2026.01.009_bib40","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/S0022-5320(63)80007-6","article-title":"The ultrastructure of normal human first trimester placenta","volume":"9","author":"Terzakis","year":"1963","journal-title":"J. Ultra. Res."},{"issue":"2","key":"10.1016\/j.placenta.2026.01.009_bib41","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1002\/path.1700930217","article-title":"The trophoblast of the human chorionic villus","volume":"93","author":"Tighe","year":"1967","journal-title":"J. Pathol. Bacteriol."},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib42","doi-asserted-by":"crossref","DOI":"10.1038\/srep42941","article-title":"Cytotrophoblast, not syncytiotrophoblast, dominates glycolysis and oxidative phosphorylation in human term placenta","volume":"7","author":"Kolahi","year":"2017","journal-title":"Sci. Rep."},{"issue":"4","key":"10.1016\/j.placenta.2026.01.009_bib43","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.tibs.2024.01.011","article-title":"Mitochondria in disease: changes in shapes and dynamics","volume":"49","author":"Jenkins","year":"2024","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.placenta.2026.01.009_bib44","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.placenta.2023.07.015","article-title":"Volume electron microscopy reveals placental ultrastructure in 3D","volume":"141","author":"Lewis","year":"2023","journal-title":"Placenta"},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib45","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-022-19723-2","article-title":"A 3D analysis revealed complexe mitochondria morphologies in porcine cumulus cells","volume":"12","author":"Lounas","year":"2022","journal-title":"Sci. Rep."},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib46","doi-asserted-by":"crossref","DOI":"10.1038\/srep30610","article-title":"The spectrum of mitochondrial ultrastructural defects in mitochondrial myopathy","volume":"6","author":"Vincent","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.placenta.2026.01.009_bib47","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.placenta.2016.12.012","article-title":"Review: placental mitochondrial function and structure in gestational disorders","volume":"54","author":"Holland","year":"2017","journal-title":"Placenta"},{"issue":"3","key":"10.1016\/j.placenta.2026.01.009_bib48","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1042\/EBC20170104","article-title":"Mitochondrial dynamics: overview of molecular mechanisms","volume":"62","author":"Tilokani","year":"2018","journal-title":"Essays Biochem."},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib49","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1038\/s41392-023-01547-9","article-title":"Mitochondrial dynamics in health and disease: mechanisms and potential targets","volume":"8","author":"Chen","year":"2023","journal-title":"Signal Transduct. Targeted Ther."},{"issue":"2","key":"10.1016\/j.placenta.2026.01.009_bib50","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1083\/jcb.30.2.424","article-title":"A new staining method (OTO) for enhancing contrast of lipid--containing membranes and droplets in osmium tetroxide--fixed tissue with osmiophilic thiocarbohydrazide(TCH)","volume":"30","author":"Seligman","year":"1966","journal-title":"J. Cell Biol."},{"issue":"4","key":"10.1016\/j.placenta.2026.01.009_bib51","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1177\/32.4.6323574","article-title":"The use of osmium-thiocarbohydrazide-osmium (OTO) and ferrocyanide-reduced osmium methods to enhance membrane contrast and preservation in cultured cells","volume":"32","author":"Willingham","year":"1984","journal-title":"J. Histochem. Cytochem."},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib52","doi-asserted-by":"crossref","first-page":"5204","DOI":"10.1038\/s41467-025-60545-3","article-title":"Reduction of SEM charging artefacts in native cryogenic biological samples","volume":"16","author":"Velazco","year":"2025","journal-title":"Nat. Commun."},{"key":"10.1016\/j.placenta.2026.01.009_bib53","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/bs.mcb.2019.04.004","article-title":"Serial-section electron microscopy using automated tape-collecting ultramicrotome (ATUM)","volume":"152","author":"Baena","year":"2019","journal-title":"Methods Cell Biol."},{"issue":"5","key":"10.1016\/j.placenta.2026.01.009_bib54","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1063\/1.1144104","article-title":"High\u2010resolution focused ion beams","volume":"64","author":"Orloff","year":"1993","journal-title":"Rev. Sci. Instrum."},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib55","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.jsb.2006.03.006","article-title":"Site-specific 3D imaging of cells and tissues with a dual beam microscope","volume":"155","author":"Heymann","year":"2006","journal-title":"J. Struct. Biol."},{"key":"10.1016\/j.placenta.2026.01.009_bib56","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2025.1586029","article-title":"A comprehensive overview of focused ion beam-scanning electron microscopy (FIB-SEM) applications for the evaluation of outer retina","volume":"13","author":"Ch","year":"2025","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.placenta.2026.01.009_bib57","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.25916","article-title":"Enhanced FIB-SEM systems for large-volume 3D imaging","volume":"6","author":"Xu","year":"2017","journal-title":"eLife"},{"issue":"12","key":"10.1016\/j.placenta.2026.01.009_bib58","doi-asserted-by":"crossref","first-page":"2959","DOI":"10.1523\/JNEUROSCI.3189-07.2008","article-title":"Serial section scanning electron microscopy of adult brain tissue using focused ion beam milling","volume":"28","author":"Knott","year":"2008","journal-title":"J. Neurosci."},{"issue":"4","key":"10.1016\/j.placenta.2026.01.009_bib59","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1002\/jmor.20970","article-title":"Evolutionary implications of fetal and maternal microvillous surfaces in epitheliochorial placentae","volume":"280","author":"Kazemian","year":"2019","journal-title":"J. Morphol."},{"issue":"4","key":"10.1016\/j.placenta.2026.01.009_bib60","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1002\/jcsm.12742","article-title":"Superiority of focused ion beam-scanning electron microscope tomography of cardiomyocytes over standard 2D analyses highlighted by unmasking mitochondrial heterogeneity","volume":"12","author":"Heinen-Weiler","year":"2021","journal-title":"J Cachexia Sarcopenia Muscle"},{"issue":"11","key":"10.1016\/j.placenta.2026.01.009_bib61","doi-asserted-by":"crossref","first-page":"2535","DOI":"10.1002\/cne.24213","article-title":"Three-dimensional analysis of somatic mitochondrial dynamics in fission-deficient injured motor neurons using FIB\/SEM","volume":"525","author":"Tamada","year":"2017","journal-title":"J. Comp. Neurol."},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib62","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1096\/fj.12-211920","article-title":"Drp1-mediated mitochondrial dynamics and survival of developing chick motoneurons during the period of normal programmed cell death","volume":"27","author":"Choi","year":"2013","journal-title":"Faseb j"},{"issue":"2","key":"10.1016\/j.placenta.2026.01.009_bib63","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1093\/jmicro\/dfaa071","article-title":"Correlation of organelle dynamics between light microscopic live imaging and electron microscopic 3D architecture using FIB-SEM","volume":"70","author":"Ohta","year":"2020","journal-title":"Microscopy"},{"issue":"9","key":"10.1016\/j.placenta.2026.01.009_bib64","article-title":"High-precision targeting workflow for volume electron microscopy","volume":"220","author":"Ronchi","year":"2021","journal-title":"JCB (J. Cell Biol.)"},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib65","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1038\/s42003-024-06045-4","article-title":"A semi-automatic method for extracting mitochondrial cristae characteristics from 3D focused ion beam scanning electron microscopy data","volume":"7","author":"Wang","year":"2024","journal-title":"Commun. Biol."},{"issue":"10","key":"10.1016\/j.placenta.2026.01.009_bib66","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1038\/s41419-020-03152-y","article-title":"OPA1 and MICOS Regulate mitochondrial crista dynamics and formation","volume":"11","author":"Hu","year":"2020","journal-title":"Cell Death Dis."},{"issue":"14","key":"10.1016\/j.placenta.2026.01.009_bib67","doi-asserted-by":"crossref","DOI":"10.15252\/embj.2019104105","article-title":"MICOS assembly controls mitochondrial inner membrane remodeling and crista junction redistribution to mediate cristae formation","volume":"39","author":"Stephan","year":"2020","journal-title":"EMBO J."},{"key":"10.1016\/j.placenta.2026.01.009_bib68","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.83623","article-title":"Cryo-plasma FIB\/SEM volume imaging of biological specimens","volume":"12","author":"Dumoux","year":"2023","journal-title":"eLife"},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib69","doi-asserted-by":"crossref","first-page":"2660","DOI":"10.1038\/s41467-024-47066-1","article-title":"Ultrastructure of human brain tissue vitrified from autopsy revealed by cryo-ET with cryo-plasma FIB milling","volume":"15","author":"Creekmore","year":"2024","journal-title":"Nat. Commun."},{"issue":"11","key":"10.1016\/j.placenta.2026.01.009_bib70","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.0020329","article-title":"Serial block-face scanning electron microscopy to reconstruct three-dimensional tissue nanostructure","volume":"2","author":"Denk","year":"2004","journal-title":"PLoS Biol."},{"key":"10.1016\/j.placenta.2026.01.009_bib71","article-title":"Serial block-face scanning electron microscopy (SBF-SEM) of biological tissue samples","volume":"169","author":"Courson","year":"2021","journal-title":"J. Vis. Exp."},{"issue":"7","key":"10.1016\/j.placenta.2026.01.009_bib72","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.1602231","article-title":"Three-dimensional positioning and structure of chromosomes in a human prophase nucleus","volume":"3","author":"Chen","year":"2017","journal-title":"Sci. Adv."},{"issue":"2","key":"10.1016\/j.placenta.2026.01.009_bib73","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1111\/joa.13191","article-title":"Serial block-face scanning electron microscopy reveals novel intercellular connections in human term placental microvasculature","volume":"237","author":"Palaiologou","year":"2020","journal-title":"J. Anat."},{"key":"10.1016\/j.placenta.2026.01.009_bib74","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.placenta.2021.11.002","article-title":"Folding of the syncytiotrophoblast basal plasma membrane increases the surface area available for exchange in human placenta","volume":"117","author":"Tashev","year":"2022","journal-title":"Placenta"},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib75","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1111\/joa.13082","article-title":"Human placental villi contain stromal macrovesicles associated with networks of stellate cells","volume":"236","author":"Palaiologou","year":"2020","journal-title":"J. Anat."},{"issue":"12","key":"10.1016\/j.placenta.2026.01.009_bib76","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2022.105453","article-title":"3D visualization of trans-syncytial nanopores provides a pathway for paracellular diffusion across the human placental syncytiotrophoblast","volume":"25","author":"Lewis","year":"2022","journal-title":"iScience"},{"issue":"2","key":"10.1016\/j.placenta.2026.01.009_bib77","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1002\/cne.25227","article-title":"The complex synaptic pathways onto a looming-detector neuron revealed using serial block-face scanning electron microscopy","volume":"530","author":"Wernitznig","year":"2022","journal-title":"J. Comp. Neurol."},{"issue":"4","key":"10.1016\/j.placenta.2026.01.009_bib78","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1016\/j.celrep.2019.01.010","article-title":"Quantitative 3D mapping of the human skeletal muscle mitochondrial network","volume":"26","author":"Vincent","year":"2019","journal-title":"Cell Rep."},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib79","doi-asserted-by":"crossref","first-page":"5111","DOI":"10.1038\/s41467-018-07676-y","article-title":"Subcellular connectomic analyses of energy networks in striated muscle","volume":"9","author":"Bleck","year":"2018","journal-title":"Nat. Commun."},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib80","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.neuron.2007.06.014","article-title":"Array tomography: a new tool for imaging the molecular architecture and ultrastructure of neural circuits","volume":"55","author":"Micheva","year":"2007","journal-title":"Neuron"},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib81","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1111\/jmi.13217","article-title":"Work smart, not hard: how array tomography can help increase the ultrastructure data output","volume":"295","author":"Kolotuev","year":"2024","journal-title":"J. Microsc."},{"issue":"8","key":"10.1016\/j.placenta.2026.01.009_bib82","doi-asserted-by":"crossref","DOI":"10.3390\/antiox11081505","article-title":"Mitochondria targeted antioxidant significantly alleviates Preeclampsia caused by 11\u03b2-HSD2 dysfunction via OPA1 and MtDNA maintenance","volume":"11","author":"Long","year":"2022","journal-title":"Antioxidants"},{"issue":"11","key":"10.1016\/j.placenta.2026.01.009_bib83","doi-asserted-by":"crossref","DOI":"10.3390\/jcm14113838","article-title":"The role of placental mitochondrial dysfunction in adverse perinatal outcomes: a systematic review","volume":"14","author":"Voros","year":"2025","journal-title":"J. Clin. Med."},{"issue":"4","key":"10.1016\/j.placenta.2026.01.009_bib84","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.1113\/JP280593","article-title":"Mitochondrial dysfunction in placental trophoblast cells experiencing gestational diabetes mellitus","volume":"599","author":"Fisher","year":"2021","journal-title":"J. Physiol."},{"issue":"7","key":"10.1016\/j.placenta.2026.01.009_bib85","doi-asserted-by":"crossref","first-page":"1366","DOI":"10.1038\/nprot.2013.078","article-title":"Studying synapses in human brain with array tomography and electron microscopy","volume":"8","author":"Kay","year":"2013","journal-title":"Nat. Protoc."},{"issue":"6696","key":"10.1016\/j.placenta.2026.01.009_bib86","doi-asserted-by":"crossref","DOI":"10.1126\/science.adk4858","article-title":"A petavoxel fragment of human cerebral cortex reconstructed at nanoscale resolution","volume":"384","author":"Shapson-Coe","year":"2024","journal-title":"Science"},{"issue":"3","key":"10.1016\/j.placenta.2026.01.009_bib87","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1016\/j.cell.2015.03.050","article-title":"A primer to single-particle cryo-electron microscopy","volume":"161","author":"Cheng","year":"2015","journal-title":"Cell"},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib88","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1038\/s41467-024-55767-w","article-title":"MPicker: visualizing and picking membrane proteins for cryo-electron tomography","volume":"16","author":"Yan","year":"2025","journal-title":"Nat. Commun."},{"issue":"10","key":"10.1016\/j.placenta.2026.01.009_bib89","doi-asserted-by":"crossref","first-page":"4250","DOI":"10.1073\/pnas.1816556116","article-title":"Dimers of mitochondrial ATP synthase induce membrane curvature and self-assemble into rows","volume":"116","author":"Blum","year":"2019","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"3","key":"10.1016\/j.placenta.2026.01.009_bib90","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1042\/BCJ20230450","article-title":"The consequence of ATP synthase dimer angle on mitochondrial morphology studied by cryo-electron tomography","volume":"481","author":"Buzzard","year":"2024","journal-title":"Biochem. J."},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib91","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1038\/s41467-022-29332-2","article-title":"Effects of cryo-EM cooling on structural ensembles","volume":"13","author":"Bock","year":"2022","journal-title":"Nat. Commun."},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib92","doi-asserted-by":"crossref","first-page":"1857","DOI":"10.1038\/s41467-022-29501-3","article-title":"Waffle Method: a general and flexible approach for improving throughput in FIB-milling","volume":"13","author":"Kelley","year":"2022","journal-title":"Nat. Commun."},{"issue":"2","key":"10.1016\/j.placenta.2026.01.009_bib93","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.jsb.2016.02.013","article-title":"An improved cryo-FIB method for fabrication of frozen hydrated lamella","volume":"194","author":"Zhang","year":"2016","journal-title":"J. Struct. Biol."},{"issue":"7","key":"10.1016\/j.placenta.2026.01.009_bib94","doi-asserted-by":"crossref","first-page":"1154","DOI":"10.1038\/emboj.2008.35","article-title":"Dimer ribbons of ATP synthase shape the inner mitochondrial membrane","volume":"27","author":"Strauss","year":"2008","journal-title":"EMBO J."},{"issue":"10","key":"10.1016\/j.placenta.2026.01.009_bib95","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1016\/j.tibs.2019.04.008","article-title":"Cryo-Electron microscopy methodology: current aspects and future directions","volume":"44","author":"Danev","year":"2019","journal-title":"Trends Biochem. Sci."},{"issue":"2","key":"10.1016\/j.placenta.2026.01.009_bib96","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.sbi.2013.02.003","article-title":"Structural biology in situ\u2014the potential of subtomogram averaging","volume":"23","author":"Briggs","year":"2013","journal-title":"Curr. Opin. Struct. Biol."},{"key":"10.1016\/j.placenta.2026.01.009_bib97","article-title":"Visualization of ATP synthase dimers in mitochondria by electron cryo-tomography","volume":"91","author":"Davies","year":"2014","journal-title":"J. Vis. Exp."},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib98","doi-asserted-by":"crossref","first-page":"5342","DOI":"10.1038\/s41467-020-18993-6","article-title":"Type III ATP synthase is a symmetry-deviated dimer that induces membrane curvature through tetramerization","volume":"11","author":"Flygaard","year":"2020","journal-title":"Nat. Commun."},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib99","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1038\/s41467-020-20381-z","article-title":"ATP synthase hexamer assemblies shape cristae of Toxoplasma mitochondria","volume":"12","author":"M\u00fchleip","year":"2021","journal-title":"Nat. Commun."},{"issue":"6713","key":"10.1016\/j.placenta.2026.01.009_bib100","doi-asserted-by":"crossref","first-page":"1086","DOI":"10.1126\/science.adp4640","article-title":"In situ structure and rotary states of mitochondrial ATP synthase in whole Polytomella cells","volume":"385","author":"Dietrich","year":"2024","journal-title":"Science"},{"issue":"7954","key":"10.1016\/j.placenta.2026.01.009_bib101","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1038\/s41586-023-05817-y","article-title":"Structural basis of mitochondrial membrane bending by the I\u2013II\u2013III2\u2013IV2 supercomplex","volume":"615","author":"M\u00fchleip","year":"2023","journal-title":"Nature"},{"issue":"8019","key":"10.1016\/j.placenta.2026.01.009_bib102","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1038\/s41586-024-07488-9","article-title":"High-resolution in situ structures of mammalian respiratory supercomplexes","volume":"631","author":"Zheng","year":"2024","journal-title":"Nature"},{"key":"10.1016\/j.placenta.2026.01.009_bib103","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.07739","article-title":"MICOS coordinates with respiratory complexes and lipids to establish mitochondrial inner membrane architecture","volume":"4","author":"Friedman","year":"2015","journal-title":"eLife"},{"issue":"6740","key":"10.1016\/j.placenta.2026.01.009_bib104","doi-asserted-by":"crossref","first-page":"1296","DOI":"10.1126\/science.ads8738","article-title":"In-cell architecture of the mitochondrial respiratory chain","volume":"387","author":"Waltz","year":"2025","journal-title":"Science"},{"key":"10.1016\/j.placenta.2026.01.009_bib105","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.isci.2018.07.014","article-title":"Three-Dimensional analysis of mitochondrial crista ultrastructure in a patient with leigh syndrome by <em>In Situ<\/em> Cryoelectron Tomography","volume":"6","author":"Siegmund","year":"2018","journal-title":"iScience"},{"issue":"3","key":"10.1016\/j.placenta.2026.01.009_bib106","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1038\/s44318-024-00027-2","article-title":"In situ architecture of Opa1-dependent mitochondrial cristae remodeling","volume":"43","author":"Fry","year":"2024","journal-title":"EMBO J."},{"key":"10.1016\/j.placenta.2026.01.009_bib107","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.55853","article-title":"Cryo-EM structures and functional properties of CALHM channels of the human placenta","volume":"9","author":"Dro\u017cd\u017cyk","year":"2020","journal-title":"eLife"},{"issue":"4","key":"10.1016\/j.placenta.2026.01.009_bib108","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1007\/s12551-019-00571-w","article-title":"Cryo-electron microscopy: an introduction to the technique, and considerations when working to establish a national facility","volume":"11","author":"Bhella","year":"2019","journal-title":"Biophysical Reviews"},{"issue":"2","key":"10.1016\/j.placenta.2026.01.009_bib109","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.tibs.2021.10.007","article-title":"Broadening access to cryoEM through centralized facilities","volume":"47","author":"Zimanyi","year":"2022","journal-title":"Trends Biochem. Sci."},{"issue":"9","key":"10.1016\/j.placenta.2026.01.009_bib110","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2021.102959","article-title":"Challenges and triumphs in cryo-electron tomography","volume":"24","author":"Hylton","year":"2021","journal-title":"iScience"},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib111","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.cels.2022.12.006","article-title":"Instance segmentation of mitochondria in electron microscopy images with a generalist deep learning model trained on a diverse dataset","volume":"14","author":"Conrad","year":"2023","journal-title":"Cell Syst."},{"key":"10.1016\/j.placenta.2026.01.009_bib112","article-title":"MemBrain v2: an end-to-end tool for the analysis of membranes in cryo-electron tomography","author":"Lamm","year":"2025","journal-title":"bioRxiv"},{"issue":"11","key":"10.1016\/j.placenta.2026.01.009_bib113","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1038\/s41592-019-0591-8","article-title":"A complete data processing workflow for cryo-ET and subtomogram averaging","volume":"16","author":"Chen","year":"2019","journal-title":"Nat. Methods"},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib114","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.1038\/s42003-025-08961-5","article-title":"cryoTIGER: deep-learning based tilt interpolation generator for enhanced reconstruction in cryo electron tomography","volume":"8","author":"Majtner","year":"2025","journal-title":"Commun. Biol."},{"issue":"1","key":"10.1016\/j.placenta.2026.01.009_bib115","doi-asserted-by":"crossref","first-page":"8833","DOI":"10.1038\/s41467-025-63895-0","article-title":"Template Learning: deep learning with domain randomization for particle picking in cryo-electron tomography","volume":"16","author":"Harastani","year":"2025","journal-title":"Nat. Commun."},{"issue":"3","key":"10.1016\/j.placenta.2026.01.009_bib116","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1016\/j.cell.2023.12.027","article-title":"Time resolution in cryo-EM using a PDMS-based microfluidic chip assembly and its application to the study of HflX-mediated ribosome recycling","volume":"187","author":"Bhattacharjee","year":"2024","journal-title":"Cell"}],"container-title":["Placenta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0143400426000214?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0143400426000214?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T12:14:20Z","timestamp":1769688860000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0143400426000214"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":116,"alternative-id":["S0143400426000214"],"URL":"https:\/\/doi.org\/10.1016\/j.placenta.2026.01.009","relation":{},"ISSN":["0143-4004"],"issn-type":[{"value":"0143-4004","type":"print"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Exploring placental ultrastructure: A review of electron microscopy techniques and emerging methods for resolving 3D organelle architecture","name":"articletitle","label":"Article Title"},{"value":"Placenta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.placenta.2026.01.009","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}