{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T14:08:46Z","timestamp":1788876526257,"version":"build-2803163510"},"reference-count":28,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Plant Science"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.plantsci.2026.113370","type":"journal-article","created":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T14:02:09Z","timestamp":1786284129000},"page":"113370","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Multiplexed CRISPR\/Cas9 mediated knockdown of BCH gene in potato enhances beta-carotene to combat vitamin A deficiency"],"prefix":"10.1016","volume":"372","author":[{"given":"Ujala","family":"Nasir","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aneela","family":"Yasmeen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Awais","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayesha","family":"Latif","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allah","family":"Bakhsh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadeem","family":"Ahmad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naila","family":"Shahid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saira","family":"Azam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul Qayyum","family":"Rao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad Ali","family":"Shahid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"26","key":"10.1016\/j.plantsci.2026.113370_bib1","doi-asserted-by":"crossref","first-page":"10839","DOI":"10.1021\/jf0726583","article-title":"Antioxidant profiling of native Andean potato tubers (Solanum tuberosum L.) reveals cultivars with high levels of \u03b2-carotene, \u03b1-tocopherol, chlorogenic acid, and petanin","volume":"55","author":"Andre","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.plantsci.2026.113370_bib2","doi-asserted-by":"crossref","first-page":"57","DOI":"10.17113\/ftb.58.01.20.6187","article-title":"Development of efficient, reproducible and stable Agrobacterium-mediated genetic transformation of five potato cultivars","volume":"58","author":"Bakhsh","year":"2020","journal-title":"Food Technol. Biotechnol."},{"key":"10.1016\/j.plantsci.2026.113370_bib3","series-title":"The potato crop, Its agricultural, nutritional and social contribution to humankin","first-page":"37","article-title":"The potato and its contribution to the human diet and health","author":"Burgos","year":"2020"},{"key":"10.1016\/j.plantsci.2026.113370_bib4","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1080\/10408390903041996","article-title":"Potatoes and human health","volume":"49","author":"Camire","year":"2009","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"10.1016\/j.plantsci.2026.113370_bib5","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0187102","article-title":"Potential of golden potatoes to improve vitamin A and vitamin E status in developing countries","volume":"12","author":"Chitchumroonchokchai","year":"2017","journal-title":"PLoS. One"},{"key":"10.1016\/j.plantsci.2026.113370_bib6","doi-asserted-by":"crossref","first-page":"63","DOI":"10.3390\/nu3010063","article-title":"Vitamin A metabolism: an update","volume":"3","author":"D\u2019Ambrosio","year":"2011","journal-title":"Nutrients"},{"key":"10.1016\/j.plantsci.2026.113370_bib7","series-title":"Advances in Root Vegetables Research","article-title":"Transgenic Approaches for Nutritional Enhancement of Potato","author":"Datir","year":"2022"},{"key":"10.1016\/j.plantsci.2026.113370_bib8","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1038\/nbt.4147","article-title":"High-throughput creation and functional profiling of DNA sequence variant libraries using CRISPR\u2013Cas9 in yeast","volume":"36","author":"Guo","year":"2018","journal-title":"Nat. Biotech."},{"key":"10.1016\/j.plantsci.2026.113370_bib9","doi-asserted-by":"crossref","DOI":"10.1155\/2022\/9363692","article-title":"Development of analytical methods for determination of \u03b2-carotene in pumpkin (Cucurbita maxima) flesh, peel, and seed powder samples","author":"Hagos","year":"2022","journal-title":"Int. J. Anal. Chem."},{"key":"10.1016\/j.plantsci.2026.113370_bib10","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1186\/s13568-021-01242-4","article-title":"CRISPR\/Cas9-induced \u03b2-carotene hydroxylase mutation in Dunaliella salina CCAP19\/18","volume":"11","author":"Hu","year":"2021","journal-title":"AMB Express"},{"key":"10.1016\/j.plantsci.2026.113370_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.algal.2021.102245","article-title":"Cloning and identification of a novel \u03b2-carotene hydroxylase gene from Haematococcus pluvialis and its function in Escherichia coli","author":"Huang","year":"2021","journal-title":"Algal Res. 55102245."},{"key":"10.1016\/j.plantsci.2026.113370_bib12","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.gendis.2018.03.004","article-title":"silico sgRNA tool design for CRISPR control of quorum sensing in Acinetobacter species","volume":"5","author":"Karlapudi","year":"2018","journal-title":"Genes. Dis."},{"key":"10.1016\/j.plantsci.2026.113370_bib13","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.phytochem.2011.11.003","article-title":"Down-regulation of \u03b2-carotene hydroxylase increases \u03b2-carotene and total carotenoids enhancing salt stress tolerance in transgenic cultured cells of sweetpotato","volume":"74","author":"Kim","year":"2012","journal-title":"Phytochem"},{"key":"10.1016\/j.plantsci.2026.113370_bib14","first-page":"2184","article-title":"Extraction and analysis of beta-carotene recovery in CPO and oil palm waste by using HPLC","volume":"11","author":"Kupan","year":"2016","journal-title":"ARPN J. Eng. Appl. Sci."},{"key":"10.1016\/j.plantsci.2026.113370_bib15","first-page":"59","article-title":"The influence of flesh colour and growing locality on polyphenolic content and antioxidant activity in potatoes","volume":"143","author":"Lachman","year":"2012","journal-title":"Sci. Hortic."},{"key":"10.1016\/j.plantsci.2026.113370_bib16","article-title":"CRISPR\/Cas9-Based Knock-Out of the PMR4 Gene Reduces Susceptibility to Late Blight in Two Tomato Cultivars","author":"Li","year":"2022","journal-title":"Int. J. Mol. Sci. 2314542."},{"key":"10.1016\/j.plantsci.2026.113370_bib17","doi-asserted-by":"crossref","DOI":"10.26444\/jpccr\/112376","article-title":"Role of vitamin A in health and illness","volume":"13","author":"Marzeda","year":"2019","journal-title":"J. Pre-Clin. Clin. Res."},{"key":"10.1016\/j.plantsci.2026.113370_bib18","first-page":"119","article-title":"Determination of carbohydrate and \u03b2-carotene content of some vegetables consumed in Kano metropolis, Nigeria","author":"Mustapha","year":"2009","journal-title":"Bayero J. Pure Appl. Sci."},{"issue":"9","key":"10.1016\/j.plantsci.2026.113370_bib19","doi-asserted-by":"crossref","first-page":"2845S","DOI":"10.1093\/jn\/132.9.2845S","article-title":"Assessment and control of vitamin A deficiency: The Annecy Accords","volume":"132","author":"Sommer","year":"2002","journal-title":"J. Nutr."},{"issue":"9","key":"10.1016\/j.plantsci.2026.113370_bib20","doi-asserted-by":"crossref","first-page":"e528","DOI":"10.1016\/S2214-109X(15)00039-X","article-title":"Trends and mortality effects of vitamin A deficiency in children in 138 low-income and middle-income countries between 1991 and 2013: a pooled analysis of population-based surveys","volume":"3","author":"Stevens","year":"2015","journal-title":"Lancet Glob. Health"},{"key":"10.1016\/j.plantsci.2026.113370_bib21","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/BF02986245","article-title":"Enhancing beta-carotene content in potato by RNAi-mediated silencing of the beta-carotene hydroxylase gene","volume":"84","author":"Van Eck","year":"2007","journal-title":"Am. J. Potato Res."},{"key":"10.1016\/j.plantsci.2026.113370_bib22","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1146\/annurev-nutr-080508-141027","article-title":"Colors with functions: elucidating the biochemical and molecular basis of carotenoid metabolism","volume":"30","author":"von Lintig","year":"2010","journal-title":"Annu. Rev. Nutr."},{"key":"10.1016\/j.plantsci.2026.113370_bib23","series-title":"WHO Global Database on Vitamin A Deficiency","article-title":"Global prevalence of vitamin A deficiency in populations at risk 1995\u20132005","author":"WHO (World Health Organization)","year":"2009"},{"issue":"17","key":"10.1016\/j.plantsci.2026.113370_bib24","doi-asserted-by":"crossref","first-page":"3703","DOI":"10.1080\/10408398.2016.1160362","article-title":"The vicious cycle of vitamin A deficiency: A review","volume":"57","author":"Wiseman","year":"2017","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"10.1016\/j.plantsci.2026.113370_bib25","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1093\/pcp\/pch115","article-title":"Overexpression of the Arabidopsis 14-3-3 protein GF14\u03bb in cotton leads to a \u201cstay-green\u201d phenotype and improves stress tolerance under moderate drought conditions","volume":"45","author":"Yan","year":"2004","journal-title":"Plant. Cell. Physiol."},{"key":"10.1016\/j.plantsci.2026.113370_bib26","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/s00425-022-04022-x","article-title":"CRISPR\/Cas-mediated knockdown of vacuolar invertase gene expression lowers the cold-induced sweetening in potatoes","volume":"256","author":"Yasmeen","year":"2022","journal-title":"Planta"},{"key":"10.1016\/j.plantsci.2026.113370_bib27","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1080\/10408398.2012.724479","article-title":"Potato production, usage, and nutrition\u2014a review","volume":"56","author":"Zaheer","year":"2016","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"10.1016\/j.plantsci.2026.113370_bib28","doi-asserted-by":"crossref","first-page":"2924","DOI":"10.1016\/S2095-3119(17)61736-2","article-title":"Progress of potato staple food research and industry development in China","volume":"16","author":"Zhang","year":"2017","journal-title":"J. Integr. Agric."}],"container-title":["Plant Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168945226003985?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168945226003985?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T13:25:43Z","timestamp":1788873943000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168945226003985"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":28,"alternative-id":["S0168945226003985"],"URL":"https:\/\/doi.org\/10.1016\/j.plantsci.2026.113370","relation":{},"ISSN":["0168-9452"],"issn-type":[{"value":"0168-9452","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Multiplexed CRISPR\/Cas9 mediated knockdown of BCH gene in potato enhances beta-carotene to combat vitamin A deficiency","name":"articletitle","label":"Article Title"},{"value":"Plant Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.plantsci.2026.113370","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"113370"}}