{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T04:08:31Z","timestamp":1791259711314,"version":"4.1.0"},"reference-count":47,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32060401"],"award-info":[{"award-number":["32060401"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004775","name":"Natural Science Foundation of Gansu Province","doi-asserted-by":"publisher","award":["23JRRA1426"],"award-info":[{"award-number":["23JRRA1426"]}],"id":[{"id":"10.13039\/501100004775","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Plant Physiology and Biochemistry"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.plaphy.2025.110083","type":"journal-article","created":{"date-parts":[[2025,5,31]],"date-time":"2025-05-31T19:45:12Z","timestamp":1748720712000},"page":"110083","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"special_numbering":"C","title":["The study on the function of exogenous NO-mediated miR166e-5p in regulating the drought resistance of alfalfa"],"prefix":"10.1016","volume":"227","author":[{"given":"Xiaolin","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bochuang","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoqiang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7869-6042","authenticated-orcid":false,"given":"Xiaohong","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.plaphy.2025.110083_bib1","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1007\/s11240-023-02517-3","article-title":"Target cleavage mapping and tissue-specific expression analysis of PGPR responsive miR166 under abiotic stress in chickpea (Cicer arietinum L","volume":"154","author":"Ankita","year":"2023","journal-title":"Plant Cell Tissue Organ Cult."},{"key":"10.1016\/j.plaphy.2025.110083_bib2","doi-asserted-by":"crossref","first-page":"1034","DOI":"10.1134\/S1021443711060033","article-title":"Stress responses of wheat leaves to dehydration: participation of endogenous NO and effect of sodium nitroprusside","volume":"58","author":"Boyarshinov","year":"2011","journal-title":"Russ. J. Plant Physiol."},{"issue":"5","key":"10.1016\/j.plaphy.2025.110083_bib3","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.3390\/ijms19051431","article-title":"Genome-wide identification of microRNAs in response to salt\/alkali stress in Medicago truncatula through high-throughput sequencing","volume":"19","author":"Cao","year":"2018","journal-title":"Int. J. Mol. Sci."},{"issue":"1","key":"10.1016\/j.plaphy.2025.110083_bib4","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s12870-019-1721-2","article-title":"Nitric oxide synthase-mediated early nitric oxide burst alleviates water stress-induced oxidative damage in ammonium-supplied rice roots","volume":"19","author":"Cao","year":"2019","journal-title":"BMC Plant Biol."},{"issue":"1","key":"10.1016\/j.plaphy.2025.110083_bib5","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1016\/j.bbrc.2017.11.149","article-title":"Functional FRIGIDA allele enhances drought tolerance by regulating the P5CS1 pathway in Arabidopsis thaliana","volume":"495","author":"Chen","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"issue":"1","key":"10.1016\/j.plaphy.2025.110083_bib6","doi-asserted-by":"crossref","first-page":"2494","DOI":"10.1038\/s41467-020-16338-x","article-title":"Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa","volume":"11","author":"Chen","year":"2020","journal-title":"Nat. Commun."},{"issue":"1","key":"10.1016\/j.plaphy.2025.110083_bib7","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.cj.2023.12.005","volume":"12","author":"Chen","year":"2024","journal-title":"Crop J."},{"issue":"7","key":"10.1016\/j.plaphy.2025.110083_bib8","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1016\/j.molp.2021.05.005","article-title":"The miR166\u2013SlHB15A regulatory module controls ovule development and parthenocarpic fruit set under adverse temperatures in tomato","volume":"14","author":"Clepet","year":"2021","journal-title":"Mol. Plant"},{"issue":"3","key":"10.1016\/j.plaphy.2025.110083_bib9","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1111\/ppl.12983","article-title":"Improving water\u2010use efficiency by changing hydraulic and stomatal characteristics in soybean exposed to drought: the involvement of nitric oxide","volume":"168","author":"de Sousa","year":"2019","journal-title":"Physiol. Plantarum"},{"key":"10.1016\/j.plaphy.2025.110083_bib10","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.indcrop.2019.05.042","article-title":"Determination of antioxidant enzyme activity and phenolic contents of some species of the asteraceae family from medicanal plants","volume":"137","author":"G\u00fcne\u015f","year":"2019","journal-title":"Ind. Crop. Prod."},{"issue":"2","key":"10.1016\/j.plaphy.2025.110083_bib11","doi-asserted-by":"crossref","first-page":"285","DOI":"10.3390\/plants9020285","article-title":"Use of nitric oxide and hydrogen peroxide for better yield of wheat (Triticum aestivum L.) under water deficit conditions: growth, osmoregulation, and antioxidative defense mechanism","volume":"9","author":"Habib","year":"2020","journal-title":"Plants"},{"key":"10.1016\/j.plaphy.2025.110083_bib12","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1007\/s00344-018-9873-0","article-title":"L-Arginine pretreatment enhances drought resistance of sunflower (Helianthus annuus L.) plants by increase in polyamines content","volume":"38","author":"Hassan","year":"2019","journal-title":"J. Plant Growth Regul."},{"issue":"5","key":"10.1016\/j.plaphy.2025.110083_bib13","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1111\/j.1365-313X.2011.04738.x","article-title":"System responses to long\u2010term drought and re\u2010watering of two contrasting alfalfa varieties","volume":"68","author":"Kang","year":"2011","journal-title":"Plant J."},{"issue":"1","key":"10.1016\/j.plaphy.2025.110083_bib14","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1186\/s12870-017-0983-9","article-title":"Conservation and diversification of the miR166 family in soybean and potential roles of newly identified miR166s","volume":"17","author":"Li","year":"2017","journal-title":"BMC Plant Biol."},{"issue":"24","key":"10.1016\/j.plaphy.2025.110083_bib15","doi-asserted-by":"crossref","first-page":"9506","DOI":"10.3390\/ijms21249506","article-title":"Maize microRNA166 inactivation confers plant development and abiotic stress resistance","volume":"21","author":"Li","year":"2020","journal-title":"Int. J. Mol. Sci."},{"issue":"5","key":"10.1016\/j.plaphy.2025.110083_bib16","first-page":"1543","article-title":"Effects of exogenous abscisic acid (ABA) on growth and physiological characteristics of Machilus yunnanensis seedlings under drought stress","volume":"31","author":"Li","year":"2020","journal-title":"Yingyong Shengtai Xuebao"},{"key":"10.1016\/j.plaphy.2025.110083_bib17","article-title":"Conservation and diversity of miR166 family members from highbush blueberry (Vaccinium corymbosum) and their potential functions in abiotic stress","volume":"13","author":"Li","year":"2022","journal-title":"Front. Genet."},{"key":"10.1016\/j.plaphy.2025.110083_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodchem.2019.124964","article-title":"Chlorophyll degradation and carotenoid biosynthetic pathways: gene expression and pigment content in broccoli during yellowing","volume":"297","author":"Luo","year":"2019","journal-title":"Food Chem."},{"key":"10.1016\/j.plaphy.2025.110083_bib19","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.pbi.2016.11.016","article-title":"The shady side of leaf development: the role of the REVOLUTA\/KANADI1 module in leaf patterning and auxin-mediated growth promotion","volume":"35","author":"Merelo","year":"2016","journal-title":"Curr. Opin. Plant Biol."},{"issue":"9","key":"10.1016\/j.plaphy.2025.110083_bib20","article-title":"Osmotic stress in banana is relieved by exogenous nitric oxide","volume":"9","author":"Muhammad","year":"2021","journal-title":"PeerJ"},{"key":"10.1016\/j.plaphy.2025.110083_bib21","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.foodchem.2003.07.013","article-title":"Purifcation and characterization of membrane-bound peroxidases from Metroxylon sagu","volume":"85","author":"Onsa","year":"2004","journal-title":"Food Chem."},{"issue":"13","key":"10.1016\/j.plaphy.2025.110083_bib22","article-title":"Intraspecific variation for leaf physiological and root morphological adaptation to drought stress in alfalfa (Medicago sativa L.)","volume":"3","author":"Prince","year":"2022","journal-title":"Front. Plant Sci."},{"issue":"10","key":"10.1016\/j.plaphy.2025.110083_bib23","doi-asserted-by":"crossref","first-page":"1556","DOI":"10.3390\/antiox10101556","article-title":"Nitric oxide prevents Fe deficiency-induced photosynthetic disturbance, and oxidative stress in alfalfa by regulating Fe acquisition and antioxidant defense","volume":"10","author":"Rahman","year":"2021","journal-title":"Antioxidants"},{"key":"10.1016\/j.plaphy.2025.110083_bib24","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.1007\/s42729-020-00198-x","article-title":"Nitric oxide stimulates antioxidant system and osmotic adjustment in soybean under drought stress","volume":"20","author":"Rezayian","year":"2020","journal-title":"J. Soil Sci. Plant Nutr."},{"issue":"12","key":"10.1016\/j.plaphy.2025.110083_bib25","article-title":"Molecular mechanisms of alfalfa (Medicago sativa L.) in response to combined drought and cold stresses","volume":"14","author":"Reziya","year":"2023","journal-title":"Agronomy"},{"issue":"15","key":"10.1016\/j.plaphy.2025.110083_bib26","doi-asserted-by":"crossref","first-page":"3384","DOI":"10.1021\/jf404627n","article-title":"Validation and application of an improved method for the rapid determination of proline in grape berries","volume":"62","author":"Rienth","year":"2014","journal-title":"J. Agric. Food Chem."},{"issue":"9","key":"10.1016\/j.plaphy.2025.110083_bib27","doi-asserted-by":"crossref","first-page":"1250","DOI":"10.1016\/j.molp.2020.07.003","article-title":"The chromosome-level genome sequence of the autotetraploid alfalfa and resequencing of core germplasms provide genomic resources for alfalfa research","volume":"13","author":"Shen","year":"2020","journal-title":"Mol. Plant"},{"issue":"3","key":"10.1016\/j.plaphy.2025.110083_bib28","first-page":"496","article-title":"Phytochemical analysis, antioxidant, acetylcholinesterase, and \u03b1-amylase inhibitors from extract","volume":"44","author":"Sher","year":"2024","journal-title":"J. Tradit. Chin. Med."},{"issue":"1","key":"10.1016\/j.plaphy.2025.110083_bib29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/agronomy7010001","article-title":"The current status, problems, and prospects of alfalfa (Medicago sativa L.) breeding in China","volume":"7","author":"Shi","year":"2017","journal-title":"Agronomy"},{"issue":"3","key":"10.1016\/j.plaphy.2025.110083_bib30","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1111\/tpj.13670","article-title":"Model for the role of auxin polar transport in patterning of the leaf adaxial\u2013abaxial axis","volume":"92","author":"Shi","year":"2017","journal-title":"Plant J."},{"issue":"4","key":"10.1016\/j.plaphy.2025.110083_bib31","doi-asserted-by":"crossref","first-page":"628","DOI":"10.3390\/f13040628","article-title":"Genome-wide investigation of the MiR166 family provides new insights into its involvement in the drought stress responses of tea plants (Camellia sinensis (L.) O. Kuntze)","volume":"13","author":"Tian","year":"2022","journal-title":"Forests"},{"issue":"4","key":"10.1016\/j.plaphy.2025.110083_bib32","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.1104\/pp.111.186395","article-title":"YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development","volume":"157","author":"Wang","year":"2011","journal-title":"Plant Physiol."},{"issue":"1","key":"10.1016\/j.plaphy.2025.110083_bib33","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/s00299-019-02473-3","article-title":"Nitric oxide and hydrogen peroxide increase glucose-6-phosphate dehydrogenase activities and expression upon drought stress in soybean roots","volume":"39","author":"Wang","year":"2019","journal-title":"Plant Cell Rep."},{"issue":"3","key":"10.1016\/j.plaphy.2025.110083_bib34","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0001829","article-title":"Highly specific gene silencing by artificial miRNAs in rice","volume":"3","author":"Warthmann","year":"2008","journal-title":"PLoS One"},{"key":"10.1016\/j.plaphy.2025.110083_bib35","article-title":"Leaf transcriptome analysis of Medicago ruthenica revealed its response and adaptive strategy to drought and drought recovery","volume":"562","author":"Wu","year":"2022","journal-title":"BMC Plant Biol."},{"issue":"1","key":"10.1016\/j.plaphy.2025.110083_bib36","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1111\/tpj.15785","article-title":"Citrus FRIGIDA cooperates with its interaction partner dehydrin to regulate drought tolerance","volume":"111","author":"Xu","year":"2022","journal-title":"Plant J."},{"issue":"11","key":"10.1016\/j.plaphy.2025.110083_bib37","doi-asserted-by":"crossref","first-page":"2471","DOI":"10.1007\/s12298-021-01096-x","article-title":"microRNA 166: an evolutionarily conserved stress biomarker in land plants targeting HD-ZIP family","volume":"27","author":"Yadav","year":"2021","journal-title":"Physiol. Mol. Biol. Plants"},{"issue":"1","key":"10.1016\/j.plaphy.2025.110083_bib38","doi-asserted-by":"crossref","first-page":"2832","DOI":"10.1038\/s41598-019-39397-7","article-title":"The interaction between miR160 and miR165\/166 in the control of leaf development and drought tolerance in arabidopsis","volume":"9","author":"Yang","year":"2019","journal-title":"Sci. Rep."},{"issue":"2","key":"10.1016\/j.plaphy.2025.110083_bib39","doi-asserted-by":"crossref","first-page":"555","DOI":"10.2134\/agronj2017.07.0396","article-title":"Protective effects of nitric oxide on photosynthetic stability and performance of Silybum marianum under water deficit conditions","volume":"110","author":"Zangani","year":"2018","journal-title":"Agron. J."},{"issue":"6","key":"10.1016\/j.plaphy.2025.110083_bib40","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.3390\/agronomy11061117","article-title":"Water stress regime of irrigated crops based on remote sensing and ground-based data","volume":"11","author":"Zeyliger","year":"2021","journal-title":"Agronomy"},{"key":"10.1016\/j.plaphy.2025.110083_bib41","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.plaphy.2019.12.014","article-title":"Analyses of microRNA166 gene structure, expression, and function during the early stage of somatic embryogenesis in Dimocarpus longan lour","volume":"147","author":"Zhang","year":"2019","journal-title":"Plant Physiol. Biochem."},{"key":"10.1016\/j.plaphy.2025.110083_bib42","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.jplph.2018.10.023","article-title":"Drought tolerance in alfalfa (Medicago sativa L.) varieties is associated with enhanced antioxidative protection and declined lipid peroxidation","volume":"232","author":"Zhang","year":"2019","journal-title":"J. Plant Physiol."},{"key":"10.1016\/j.plaphy.2025.110083_bib43","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1016\/j.plaphy.2019.06.023","article-title":"Endogenous NO-mediated transcripts involved in photosynthesis and carbohydrate metabolism in alfalfa (Medicago sativa L.) seedlings under drought stress","volume":"141","author":"Zhao","year":"2019","journal-title":"Plant Physiol. Biochem."},{"issue":"1","key":"10.1016\/j.plaphy.2025.110083_bib44","doi-asserted-by":"crossref","first-page":"30","DOI":"10.3390\/genes11010030","article-title":"Identification of exogenous nitric oxide-responsive miRNAs from alfalfa (Medicago sativa L.) under drought stress by high-throughput sequencing","volume":"11","author":"Zhao","year":"2019","journal-title":"Genes"},{"key":"10.1016\/j.plaphy.2025.110083_bib45","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1016\/j.plaphy.2019.06.023","article-title":"Endogenous NO-mediated transcripts involved in photosynthesis and carbohydrate metabolism in alfalfa (Medicago sativa L.) seedlings under drought stress","volume":"141","author":"Zhao","year":"2019","journal-title":"Plant Physiol. Biochem."},{"issue":"5","key":"10.1016\/j.plaphy.2025.110083_bib46","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1007\/s00709-020-01508-x","article-title":"Transcriptional analysis reveals sodium nitroprusside affects alfalfa in response to PEG-Induced osmotic stress at germination stage","volume":"257","author":"Zhao","year":"2020","journal-title":"Protoplasma"},{"issue":"5","key":"10.1016\/j.plaphy.2025.110083_bib47","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1111\/jipb.13253","article-title":"The miR166 mediated regulatory module controls plant height by regulating gibberellic acid biosynthesis and catabolism in soybean","volume":"64","author":"Zhao","year":"2022","journal-title":"J. Integr. Plant Biol."}],"container-title":["Plant Physiology and Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0981942825006114?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0981942825006114?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T15:36:52Z","timestamp":1757605012000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0981942825006114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":47,"alternative-id":["S0981942825006114"],"URL":"https:\/\/doi.org\/10.1016\/j.plaphy.2025.110083","relation":{},"ISSN":["0981-9428"],"issn-type":[{"value":"0981-9428","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The study on the function of exogenous NO-mediated miR166e-5p in regulating the drought resistance of alfalfa","name":"articletitle","label":"Article Title"},{"value":"Plant Physiology and Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.plaphy.2025.110083","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Published by Elsevier Masson SAS.","name":"copyright","label":"Copyright"}],"article-number":"110083"}}