{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,12]],"date-time":"2026-11-12T21:28:09Z","timestamp":1794518889324,"version":"4.9.1"},"reference-count":54,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Beijing Natural Science Foundation","doi-asserted-by":"publisher","award":["6244055"],"award-info":[{"award-number":["6244055"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003352","name":"Beijing University of Agriculture","doi-asserted-by":"publisher","award":["KFKT-2025010"],"award-info":[{"award-number":["KFKT-2025010"]}],"id":[{"id":"10.13039\/501100003352","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005196","name":"Chinese Academy of Agricultural Sciences","doi-asserted-by":"publisher","award":["CAAS-ZRC-ZYZX20230204"],"award-info":[{"award-number":["CAAS-ZRC-ZYZX20230204"]}],"id":[{"id":"10.13039\/501100005196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Plant Physiology and Biochemistry"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.plaphy.2026.111762","type":"journal-article","created":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T15:23:29Z","timestamp":1789831409000},"page":"111762","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Cloning and functional analysis of downy mildew resistance gene RPF1 in spinach (Spinacia oleracea L.)"],"prefix":"10.1016","volume":"239","author":[{"given":"Hao","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaosheng","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiyu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0737-6045","authenticated-orcid":false,"given":"Hongbing","family":"She","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8919-2747","authenticated-orcid":false,"given":"Wei","family":"Qian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.plaphy.2026.111762_bib1","doi-asserted-by":"crossref","DOI":"10.3389\/fpls.2020.563187","article-title":"Genome wide association studies in multiple spinach breeding populations refine downy mildew race 13 resistance genes","volume":"11","author":"Bhattarai","year":"2020","journal-title":"Front. Plant Sci."},{"key":"10.1016\/j.plaphy.2026.111762_bib2","doi-asserted-by":"crossref","DOI":"10.1093\/hr\/uhad076","article-title":"Skim resequencing finely maps the downy mildew resistance loci RPF2 and RPF3 in spinach cultivars Whale and Lazio","volume":"10","author":"Bhattarai","year":"2023","journal-title":"Hortic. Res."},{"key":"10.1016\/j.plaphy.2026.111762_bib3","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/j.molp.2014.12.022","article-title":"Signaling mechanisms in pattern-triggered immunity (PTI)","volume":"8","author":"Bigeard","year":"2015","journal-title":"Mol. Plant"},{"key":"10.1016\/j.plaphy.2026.111762_bib4","doi-asserted-by":"crossref","first-page":"5825","DOI":"10.1093\/molbev\/msab293","article-title":"eggNOG-mapper v2: functional annotation, orthology assignments, and domain prediction at the metagenomic scale","volume":"38","author":"Cantalapiedra","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.plaphy.2026.111762_bib5","doi-asserted-by":"crossref","DOI":"10.1002\/imt2.107","article-title":"Ultrafast one-pass FASTQ data preprocessing, quality control, and deduplication using fastp","volume":"2","author":"Chen","year":"2023","journal-title":"iMeta"},{"key":"10.1016\/j.plaphy.2026.111762_bib6","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: an ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"10.1016\/j.plaphy.2026.111762_bib7","doi-asserted-by":"crossref","first-page":"7762","DOI":"10.1093\/nar\/gkv784","article-title":"High speed BLASTN: an accelerated MegaBLAST search tool","volume":"43","author":"Chen","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.plaphy.2026.111762_bib8","doi-asserted-by":"crossref","first-page":"198","DOI":"10.3389\/fpls.2012.00198","article-title":"Syntenic gene analysis between Brassica rapa and other Brassicaceae species","volume":"3","author":"Cheng","year":"2012","journal-title":"Front. Plant Sci."},{"key":"10.1016\/j.plaphy.2026.111762_bib9","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1111\/j.1467-985X.2009.00624_12.x","article-title":"Lattice: multivariate data visualization with R","author":"Cheshire","year":"2010","journal-title":"J. R. Stat. Soc. Ser. A Stat. Soc"},{"key":"10.1016\/j.plaphy.2026.111762_bib10","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1007\/s10658-010-9713-y","article-title":"Spinach: better management of downy mildew and white rust through genomics","volume":"129","author":"Correll","year":"2011","journal-title":"Eur. J. Plant Pathol."},{"key":"10.1016\/j.plaphy.2026.111762_bib11","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1016\/j.tplants.2020.02.013","article-title":"Virus-induced gene silencing: Hush genes to make them talk","volume":"25","author":"Courdavault","year":"2020","journal-title":"Trends Plant Sci."},{"key":"10.1016\/j.plaphy.2026.111762_bib12","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1146\/annurev-arplant-050213-040012","article-title":"Effector-triggered immunity: from pathogen perception to robust defense","volume":"66","author":"Cui","year":"2015","journal-title":"Annu. Rev. Plant Biol."},{"key":"10.1016\/j.plaphy.2026.111762_bib13","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1186\/1471-2105-7-191","article-title":"STEM: a tool for the analysis of short time series gene expression data","volume":"7","author":"Ernst","year":"2006","journal-title":"BMC Bioinf."},{"key":"10.1016\/j.plaphy.2026.111762_bib14","doi-asserted-by":"crossref","first-page":"i159","DOI":"10.1093\/bioinformatics\/bti1022","article-title":"Clustering short time series gene expression data","volume":"1","author":"Ernst","year":"2005","journal-title":"Bioinformatics"},{"key":"10.1016\/j.plaphy.2026.111762_bib15","doi-asserted-by":"crossref","DOI":"10.1007\/s10681-018-2258-4","article-title":"Development of molecular markers linked to three spinach downy mildew resistance loci","volume":"214","author":"Feng","year":"2018","journal-title":"Euphytica"},{"key":"10.1016\/j.plaphy.2026.111762_bib16","doi-asserted-by":"crossref","first-page":"1996","DOI":"10.1007\/s11105-015-0891-9","article-title":"Construction of a spinach bacterial artificial chromosome (BAC) library as a resource for gene identification and marker development","volume":"33","author":"Feng","year":"2015","journal-title":"Plant Mol. Biol. Rep."},{"key":"10.1016\/j.plaphy.2026.111762_bib17","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1094\/PDIS-01-25-0064-RE","article-title":"Phenotypic and genotypic characterization of novel strains of spinach downy mildew pathogen","volume":"110","author":"Feng","year":"2026","journal-title":"Plant Dis."},{"key":"10.1016\/j.plaphy.2026.111762_bib18","doi-asserted-by":"crossref","DOI":"10.3390\/ijms232314872","article-title":"Fine mapping and identification of a candidate gene of downy mildew resistance, RPF2, in spinach (Spinacia oleracea L.)","volume":"23","author":"Gao","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.plaphy.2026.111762_bib19","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1094\/PHYTO-98-8-0894","article-title":"Characterization of a resistance locus (Pfs-1) to the spinach downy mildew pathogen (Peronospora farinosa f. sp spinaciae) and development of a molecular marker linked to Pfs-1","volume":"98","author":"Irish","year":"2008","journal-title":"Phytopathology"},{"key":"10.1016\/j.plaphy.2026.111762_bib20","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1094\/PDIS-10-18-1720-FE","article-title":"Spinach downy mildew: Advances in our understanding of the disease cycle and prospects for disease management","volume":"103","author":"Kandel","year":"2019","journal-title":"Plant Dis."},{"key":"10.1016\/j.plaphy.2026.111762_bib21","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: a fast spliced aligner with low memory requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nat. Methods"},{"key":"10.1016\/j.plaphy.2026.111762_bib22","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.3001124","article-title":"RefPlantNLR is a comprehensive collection of experimentally validated plant disease resistance proteins from the NLR family","volume":"19","author":"Kourelis","year":"2021","journal-title":"PLoS Biol."},{"key":"10.1016\/j.plaphy.2026.111762_bib23","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1094\/PDIS-06-15-0672-RE","article-title":"Plasmolysis and vital staining reveal viable oospores of Peronospora effusa in spinach seed lots","volume":"100","author":"Kunjeti","year":"2016","journal-title":"Plant Dis."},{"key":"10.1016\/j.plaphy.2026.111762_bib24","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1186\/1471-2105-9-559","article-title":"WGCNA: an R package for weighted correlation network analysis","volume":"9","author":"Langfelder","year":"2008","journal-title":"BMC Bioinf."},{"key":"10.1016\/j.plaphy.2026.111762_bib25","doi-asserted-by":"crossref","first-page":"11","DOI":"10.18637\/jss.v046.i11","article-title":"Fast R functions for robust correlations and hierarchical clustering","volume":"46","author":"Langfelder","year":"2012","journal-title":"J. Stat. Software"},{"key":"10.1016\/j.plaphy.2026.111762_bib26","series-title":"Aligning Sequence Reads, Clone Sequences and Assembly Contigs with BWA-MEM","author":"Li","year":"2013"},{"key":"10.1016\/j.plaphy.2026.111762_bib27","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","article-title":"The sequence alignment\/map format and SAMtools","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"},{"key":"10.1016\/j.plaphy.2026.111762_bib28","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1093\/bioinformatics\/btt656","article-title":"featureCounts: an efficient general purpose program for assigning sequence reads to genomic features","volume":"30","author":"Liao","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.plaphy.2026.111762_bib29","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"10.1016\/j.plaphy.2026.111762_bib30","author":"Maechler"},{"key":"10.1016\/j.plaphy.2026.111762_bib31","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1101\/gr.107524.110","article-title":"The genome analysis toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data","volume":"20","author":"McKenna","year":"2010","journal-title":"Genome Res."},{"key":"10.1016\/j.plaphy.2026.111762_bib32","doi-asserted-by":"crossref","first-page":"12273","DOI":"10.1073\/pnas.0900992106","article-title":"Genomewide SNP variation reveals relationships among landraces and modern varieties of rice","volume":"106","author":"McNally","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.plaphy.2026.111762_bib33","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1038\/s41477-026-02237-4","article-title":"High-efficiency, transgene-free plant genome editing by viral delivery of an engineered TnpB","volume":"12","author":"Nagalakshmi","year":"2026","journal-title":"Nat. Plants"},{"key":"10.1016\/j.plaphy.2026.111762_bib34","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.tplants.2021.08.012","article-title":"Plant immune networks","volume":"27","author":"Ngou","year":"2022","journal-title":"Trends Plant Sci."},{"key":"10.1016\/j.plaphy.2026.111762_bib35","article-title":"Scaling accurate genetic variant discovery to tens of thousands of samples","author":"Poplin","year":"2017","journal-title":"bioRxiv"},{"key":"10.1016\/j.plaphy.2026.111762_bib36","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1094\/PDIS-08-15-0847-PDN","article-title":"First report of race diversity of the spinach downy mildew pathogen, Peronospora effusa, in China","volume":"100","author":"Qian","year":"2016","journal-title":"Plant Dis."},{"key":"10.1016\/j.plaphy.2026.111762_bib37","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1093\/plphys\/kiad389","article-title":"Evolution of the spinach sex-linked region within a rarely recombining pericentromeric region","volume":"193","author":"She","year":"2023","journal-title":"Plant Physiol."},{"key":"10.1016\/j.plaphy.2026.111762_bib38","doi-asserted-by":"crossref","first-page":"3102","DOI":"10.1111\/pbi.14433","article-title":"Pan-genome analysis of 13 Spinacia accessions reveals structural variations associated with sex chromosome evolution and domestication traits in spinach","volume":"22","author":"She","year":"2024","journal-title":"Plant Biotechnol. J."},{"key":"10.1016\/j.plaphy.2026.111762_bib39","doi-asserted-by":"crossref","DOI":"10.1016\/j.xplc.2025.101410","article-title":"A complete telomere-to-telomere assembly of the Spinacia oleracea genome reveals Y chromosome evolution and centromere landscape","volume":"6","author":"She","year":"2025","journal-title":"Plant Commun."},{"key":"10.1016\/j.plaphy.2026.111762_bib40","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1111\/nph.19799","article-title":"Insights into spinach domestication from genome sequences of two wild spinach progenitors, Spinacia turkestanica and Spinacia tetrandra","volume":"243","author":"She","year":"2024","journal-title":"New Phytol."},{"key":"10.1016\/j.plaphy.2026.111762_bib41","doi-asserted-by":"crossref","first-page":"2529","DOI":"10.1007\/s00122-018-3169-4","article-title":"Fine mapping and candidate gene screening of the downy mildew resistance gene RPF1 in spinach","volume":"131","author":"She","year":"2018","journal-title":"Theor. Appl. Genet."},{"key":"10.1016\/j.plaphy.2026.111762_bib42","doi-asserted-by":"crossref","DOI":"10.3389\/fpls.2026.1766206","article-title":"Pan-NLRome of Spinacia facilitates the rapid discovery of downy mildew resistance genes","volume":"17","author":"She","year":"2026","journal-title":"Front. Plant Sci."},{"key":"10.1016\/j.plaphy.2026.111762_bib43","doi-asserted-by":"crossref","first-page":"1614","DOI":"10.1111\/jipb.13888","article-title":"Wheat MAPK cascade mediates SGT1 nuclear entry targeted by a stripe rust effector","volume":"67","author":"Shu","year":"2025","journal-title":"J. Integr. Plant Biol."},{"key":"10.1016\/j.plaphy.2026.111762_bib44","doi-asserted-by":"crossref","first-page":"1622","DOI":"10.1111\/1462-2920.15944","article-title":"Sexual reproduction contributes to the evolution of resistance-breaking isolates of the spinach pathogen Peronospora effusa","volume":"24","author":"Skiadas","year":"2022","journal-title":"Environ. Microbiol."},{"key":"10.1016\/j.plaphy.2026.111762_bib45","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1105\/tpc.16.00891","article-title":"Receptor kinases in plant-pathogen interactions: more than pattern recognition","volume":"29","author":"Tang","year":"2017","journal-title":"Plant Cell"},{"key":"10.1016\/j.plaphy.2026.111762_bib46","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1094\/PHYTO-05-15-0127-RVW","article-title":"Evolution, diversity, and taxonomy of the Peronosporaceae, with focus on the genus Peronospora","volume":"106","author":"Thines","year":"2016","journal-title":"Phytopathology"},{"key":"10.1016\/j.plaphy.2026.111762_bib47","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1007\/s10722-019-00792-8","article-title":"Acquisition and regeneration of Spinacia turkestanica Iljin and S. tetrandra Steven ex M. Bieb. to improve a spinach gene bank collection","volume":"67","author":"Van Treuren","year":"2020","journal-title":"Genet. Resour. Crop Evol."},{"key":"10.1016\/j.plaphy.2026.111762_bib48","series-title":"ggplot2: Elegant Graphics for Data Analysis","author":"Wickham","year":"2016"},{"key":"10.1016\/j.plaphy.2026.111762_bib49","first-page":"11","article-title":"clusterProfiler 4.0: a universal enrichment tool for interpreting omics data","volume":"2","author":"Wu","year":"2021","journal-title":"Innovation"},{"key":"10.1016\/j.plaphy.2026.111762_bib50","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1111\/jipb.12123","article-title":"Receptor-like kinases in plant innate immunity","volume":"55","author":"Wu","year":"2012","journal-title":"J. Integr. Plant Biol."},{"key":"10.1016\/j.plaphy.2026.111762_bib51","article-title":"Draft genome of spinach and transcriptome diversity of 120 Spinacia accessions","volume":"8","author":"Xu","year":"2017","journal-title":"Nat. Commun."},{"key":"10.1016\/j.plaphy.2026.111762_bib52","doi-asserted-by":"crossref","DOI":"10.53388\/2025825002","article-title":"Abnormal activation of the Ras\/MAPK signaling pathway in oncogenesis and progression","volume":"8","author":"Xu","year":"2025","journal-title":"Cancer Adv."},{"key":"10.1016\/j.plaphy.2026.111762_bib53","doi-asserted-by":"crossref","DOI":"10.1093\/hr\/uhad083","article-title":"RcSPL1-RcTAF15b regulates the flowering time of rose (Rosa chinensis)","volume":"10","author":"Yu","year":"2023","journal-title":"Hortic. Res."},{"key":"10.1016\/j.plaphy.2026.111762_bib54","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1111\/nph.19139","article-title":"PIF3 is phosphorylated by MAPK to modulate plant immunity","volume":"240","author":"Zhao","year":"2023","journal-title":"New Phytol."}],"container-title":["Plant Physiology and Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0981942826007485?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0981942826007485?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,11,12]],"date-time":"2026-11-12T20:45:09Z","timestamp":1794516309000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0981942826007485"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":54,"alternative-id":["S0981942826007485"],"URL":"https:\/\/doi.org\/10.1016\/j.plaphy.2026.111762","relation":{},"ISSN":["0981-9428"],"issn-type":[{"value":"0981-9428","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Cloning and functional analysis of downy mildew resistance gene RPF1 in spinach (Spinacia oleracea L.)","name":"articletitle","label":"Article Title"},{"value":"Plant Physiology and Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.plaphy.2026.111762","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Masson SAS.","name":"copyright","label":"Copyright"}],"article-number":"111762"}}